FastQCFastQC Report
Thu 26 May 2016
SRR937965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584342
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106650.6731501153160113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91770.5792309993675607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58330.36816545922534405No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.14902085534562612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.11443236371944947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17570.11089777333429274No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17270.10900424277081588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43100.056.2135351
GTATCAA74100.041.3513341
ATCAACG95800.031.831913
TATCAAC96600.031.6706332
TCAACGC97150.031.485374
CAACGCA98500.030.9574075
GTGGTAT18650.030.566831
AACGCAG101550.030.167936
TGGTATC18450.030.1257232
TAAGGTG9050.029.390385
ACGCAGA114550.026.4954667
CGCAGAG114500.026.4240688
GTAAGGT9550.025.3648664
GAGTACT78700.024.20111712-13
GCAGAGT124450.024.044269
GTACTTT82000.023.16924714-15
GGTAAGG9750.022.4102333
TACATGG68550.022.3149762
GTACATG69800.022.2556511
CAGAGTA121000.021.98206110-11
Produced by FastQC (version 0.11.5)