FastQCFastQC Report
Thu 26 May 2016
SRR937964_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937964_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663119
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110680.6654965760116984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93970.565022707334833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54970.3305235524337104No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.14130077282503536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18460.11099626665319799No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17770.10684743545110122No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17290.10396129200616433No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16870.10143591649184455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.056.431751
GTATCAA76500.043.3353081
ATCAACG98050.033.520353
TATCAAC99450.033.2367742
TCAACGC99100.033.0692024
CAACGCA100750.032.527625
AACGCAG104050.031.5350466
TGGTATC18900.030.9515322
GTGGTAT19550.029.9676321
ACGCAGA120000.027.1445527
CGCAGAG120650.026.995878
GCAGAGT129300.024.7110829
GAGTACT78550.024.21150412-13
GTACATG79800.023.8157421
TACATGG78300.023.6279832
GTACTTT83350.022.50007214-15
CAGAGTA124950.022.14496610-11
TAAGGTG9700.022.0657625
ACATGGG80650.021.8800183
AGTACTT80000.021.63312712-13
Produced by FastQC (version 0.11.5)