##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937964_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1663119 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.916451558788037 33.0 31.0 34.0 30.0 34.0 2 32.02978740547129 33.0 31.0 34.0 30.0 34.0 3 32.044167615185685 34.0 31.0 34.0 30.0 34.0 4 35.57141731890502 37.0 35.0 37.0 33.0 37.0 5 35.58140277394462 37.0 35.0 37.0 33.0 37.0 6 35.61526745831176 37.0 35.0 37.0 33.0 37.0 7 35.54576912415768 37.0 35.0 37.0 33.0 37.0 8 35.538508068274126 37.0 35.0 37.0 33.0 37.0 9 37.16063733262623 39.0 37.0 39.0 34.0 39.0 10-11 37.06336287421405 39.0 37.0 39.0 33.0 39.0 12-13 36.903008143133476 39.0 37.0 39.0 33.0 39.0 14-15 38.04792892150231 40.0 38.0 41.0 33.0 41.0 16-17 37.86086804371786 40.0 38.0 41.0 33.0 41.0 18-19 38.063487339150115 40.0 38.0 41.0 33.0 41.0 20-21 38.07333359789648 40.0 38.0 41.0 33.0 41.0 22-23 37.95194751548145 40.0 38.0 41.0 33.0 41.0 24-25 37.81972426507063 40.0 38.0 41.0 32.5 41.0 26-27 37.62446072710371 40.0 37.5 41.0 32.0 41.0 28-29 37.43025784685281 40.0 37.0 41.0 32.0 41.0 30-31 37.17145044942665 40.0 37.0 41.0 31.0 41.0 32-33 36.83980881704797 39.5 36.0 41.0 30.5 41.0 34-35 36.58128462244734 39.0 35.0 41.0 30.0 41.0 36-37 36.296273748300635 39.0 35.0 41.0 30.0 41.0 38-39 35.978023520866515 39.0 35.0 40.0 29.0 41.0 40-41 35.819489765915726 39.0 35.0 40.0 28.5 41.0 42-43 35.56207914166094 39.0 35.0 40.0 27.0 41.0 44-45 35.187772191887646 38.0 35.0 40.0 25.5 41.0 46-47 34.74127227215851 38.0 34.0 40.0 24.0 41.0 48-49 34.27518806531583 38.0 33.5 40.0 22.5 41.0 50-51 33.52301248437424 37.5 33.0 39.5 20.5 40.5 52-53 33.57862967111794 38.0 33.0 39.5 20.5 40.5 54-55 33.64396173695328 38.0 33.0 40.0 19.5 41.0 56-57 33.3055511962764 37.5 33.0 40.0 16.5 41.0 58-59 32.87829824564568 37.0 32.5 40.0 11.5 41.0 60-61 32.35770140320687 36.5 31.5 39.5 8.0 41.0 62-63 31.789332573315562 36.0 31.0 39.0 4.5 41.0 64-65 31.23167133560497 35.0 30.5 39.0 2.0 40.0 66-67 30.632031742767655 35.0 29.5 38.5 2.0 40.0 68-69 29.926956519647725 35.0 28.5 38.0 2.0 40.0 70-71 29.317302910976302 34.0 28.0 37.0 2.0 39.5 72-73 28.59805642290179 34.0 26.5 36.5 2.0 39.0 74-75 27.89788794427819 34.0 26.0 36.0 2.0 39.0 76-77 27.12488072110294 33.0 24.5 35.0 2.0 37.5 78-79 26.335286891677626 32.5 22.5 35.0 2.0 37.0 80-81 25.589376947771022 32.0 19.5 35.0 2.0 36.5 82-83 24.826319102842312 31.0 16.5 35.0 2.0 36.0 84-85 24.070990109547182 31.0 8.5 34.0 2.0 35.5 86-87 23.29816327033724 30.5 3.5 34.0 2.0 35.0 88-89 22.378792798350567 29.5 2.0 34.0 2.0 35.0 90-91 21.445628063896812 29.0 2.0 34.0 2.0 35.0 92-93 20.47510250318829 28.0 2.0 34.0 2.0 35.0 94-95 19.371376311616906 26.0 2.0 33.5 2.0 35.0 96-97 18.088395358359804 24.5 2.0 33.0 2.0 34.5 98-99 16.52240188465167 10.5 2.0 32.0 2.0 34.0 100-101 12.730835857205648 2.0 2.0 28.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15806567088255008 1101 2 0.16675725461218605 1101 3 0.11690823168462572 1101 4 0.0580191255425504 1101 5 0.09816096059523005 1101 6 0.15874488256157093 1101 7 0.1595959124487294 1101 8 0.24552286090797537 1101 9 0.30102739916495125 1101 10-11 0.28627577419592143 1101 12-13 0.2156502325149816 1101 14-15 0.47725587810871417 1101 16-17 0.4232709663922307 1101 18-19 0.45840335876604854 1101 20-21 0.5289447221589754 1101 22-23 0.3470614612864722 1101 24-25 0.3337312579099674 1101 26-27 0.42656048719753414 1101 28-29 0.4154722587586832 1101 30-31 0.28118713243961224 1101 32-33 0.24504716381696312 1101 34-35 0.15744467812744745 1101 36-37 0.13700471007921777 1101 38-39 0.1128037398697117 1101 40-41 0.42313582016550555 1101 42-43 0.3266522372367078 1101 44-45 0.21316495037610395 1101 46-47 0.22948410698766253 1101 48-49 0.21144395976627806 1101 50-51 0.31138808686059605 1101 52-53 0.18454976056511185 1101 54-55 0.15810364156187973 1101 56-57 0.13382444039829267 1101 58-59 0.1855939612171369 1101 60-61 0.25892292597978894 1101 62-63 0.10069650486927628 1101 64-65 0.03484863354477241 1101 66-67 -0.012838707211376033 1101 68-69 -0.09759830547067239 1101 70-71 -0.25721758741792655 1101 72-73 -0.22778290895006492 1101 74-75 -0.25663391413903014 1101 76-77 -0.5158493675901532 1101 78-79 -0.46079105547319443 1101 80-81 -0.37561497596448135 1101 82-83 -0.5552100629649495 1101 84-85 -0.6922218929366473 1101 86-87 -0.6112843055978523 1101 88-89 -0.33320291586374395 1101 90-91 -0.5034613041286278 1101 92-93 -0.6248508447332952 1101 94-95 -0.8625003439479038 1101 96-97 -1.106413924047974 1101 98-99 -1.2728281562511246 1101 100-101 -1.3576511621240925 1102 1 0.10140361008507881 1102 2 0.059548770242230376 1102 3 0.037734237498789014 1102 4 0.06529310896141993 1102 5 0.09694160158866083 1102 6 0.03711045697925641 1102 7 0.02831518173056935 1102 8 0.22396111235254068 1102 9 0.031854122776906024 1102 10-11 0.14619663698888274 1102 12-13 0.09580222619835865 1102 14-15 0.11910752724914886 1102 16-17 0.1520878561743828 1102 18-19 0.08883080735378002 1102 20-21 0.0724630080578379 1102 22-23 0.16615622854052958 1102 24-25 -0.016506961233694994 1102 26-27 0.08193454957416435 1102 28-29 -0.008825645271748783 1102 30-31 0.21742874317786232 1102 32-33 0.15936812710160098 1102 34-35 -0.010164247688152273 1102 36-37 -0.1684142146596841 1102 38-39 -0.3751045076269719 1102 40-41 -0.21343777205355963 1102 42-43 0.11211772910765205 1102 44-45 -0.09160571322795619 1102 46-47 -0.06734502124691488 1102 48-49 -0.32484924268858606 1102 50-51 -0.35461521500926807 1102 52-53 -0.5196542914034481 1102 54-55 -0.6466410018720694 1102 56-57 -0.44481255131115205 1102 58-59 -0.413022932154 1102 60-61 -0.36904382203729114 1102 62-63 -0.563071305488549 1102 64-65 -0.5911849952819885 1102 66-67 -0.5394227972232208 1102 68-69 -0.6915670094867501 1102 70-71 -1.0025702953101145 1102 72-73 -0.7725604813579174 1102 74-75 -0.5911519129187717 1102 76-77 -0.7176819950889843 1102 78-79 -0.7464947664878032 1102 80-81 -0.8013991700570067 1102 82-83 -0.826700928987993 1102 84-85 -0.914337243042521 1102 86-87 -0.8763867712332853 1102 88-89 -0.9348224471417836 1102 90-91 -0.787331490397154 1102 92-93 -0.80536756942195 1102 94-95 -0.6895855465463292 1102 96-97 -0.9641665888722919 1102 98-99 -1.2845740199413225 1102 100-101 -1.4939021939584247 1103 1 0.3141243394092541 1103 2 0.344215985139968 1103 3 0.36277159679351456 1103 4 0.2430826766546943 1103 5 0.250770818767748 1103 6 0.21442960704424507 1103 7 0.257024804954348 1103 8 0.24033995770717098 1103 9 0.32820131220842796 1103 10-11 0.27845782488181214 1103 12-13 0.2904874796668011 1103 14-15 0.35781175658658526 1103 16-17 0.3579857614011459 1103 18-19 0.3258372888598231 1103 20-21 0.32796433000211067 1103 22-23 0.4658661966193378 1103 24-25 0.35270455869272865 1103 26-27 0.4352164199263555 1103 28-29 0.3878575567513991 1103 30-31 0.3879065000285422 1103 32-33 0.24302244088431735 1103 34-35 0.1848075007268406 1103 36-37 0.2580666724135554 1103 38-39 0.2888722898193308 1103 40-41 0.30103243335624796 1103 42-43 0.3072672104987433 1103 44-45 0.3369443040147928 1103 46-47 0.2888888965714784 1103 48-49 0.2216208566119704 1103 50-51 0.06222122644054906 1103 52-53 0.21241634198028692 1103 54-55 0.14992724847500227 1103 56-57 0.20120702584353722 1103 58-59 0.2321093064856754 1103 60-61 0.03305006695475754 1103 62-63 -0.15124587287233737 1103 64-65 -0.24519282968083544 1103 66-67 -0.06033250710287419 1103 68-69 0.14244277024815233 1103 70-71 -0.20345586533779425 1103 72-73 -0.3830520324097222 1103 74-75 -0.29204234628659265 1103 76-77 -0.017268611176916693 1103 78-79 -0.2613655342765746 1103 80-81 -0.33815275787566534 1103 82-83 -0.4478900598648963 1103 84-85 -0.761178724681205 1103 86-87 -1.0551733236556693 1103 88-89 -0.9669218184445398 1103 90-91 -1.008702333346637 1103 92-93 -1.145345109632931 1103 94-95 -1.1086737144818848 1103 96-97 -1.3264257430048048 1103 98-99 -1.6438174610639589 1103 100-101 -1.5888425517234115 1104 1 0.27224575118017214 1104 2 0.24239958531915562 1104 3 0.2438018222739302 1104 4 0.16283422002116765 1104 5 0.13484446475911938 1104 6 0.25692529436310707 1104 7 0.27548374022741484 1104 8 0.3481974111174253 1104 9 0.3170873411015336 1104 10-11 0.4162600528735325 1104 12-13 0.2556655185291916 1104 14-15 0.30503142818756857 1104 16-17 0.34900111215350194 1104 18-19 0.3743888797462276 1104 20-21 0.33534559411474874 1104 22-23 0.3894251093699381 1104 24-25 0.31853084034896995 1104 26-27 0.36736171376204396 1104 28-29 0.15019273230701202 1104 30-31 0.23954655635945699 1104 32-33 0.1698372113325206 1104 34-35 0.14105637150975525 1104 36-37 0.2555075261883033 1104 38-39 0.3447294511800223 1104 40-41 0.23668830326383272 1104 42-43 0.5653078548887933 1104 44-45 0.4807434481610571 1104 46-47 0.18297142779974251 1104 48-49 0.08268838028737946 1104 50-51 0.10181147429492654 1104 52-53 0.2320521380174867 1104 54-55 0.11964834938638091 1104 56-57 0.21299172096110652 1104 58-59 -0.07981448342673758 1104 60-61 0.0858605078093575 1104 62-63 0.0751713306622861 1104 64-65 -0.01630970478344196 1104 66-67 -0.03625347386945066 1104 68-69 0.2662975688123268 1104 70-71 0.23460343227474567 1104 72-73 0.1229869586827661 1104 74-75 -0.018623724579061474 1104 76-77 -0.21833401460544266 1104 78-79 -0.15656944510080706 1104 80-81 -0.16618444277893119 1104 82-83 -0.2813987054396456 1104 84-85 -0.05371854943496146 1104 86-87 -0.11574370306436066 1104 88-89 -0.19343843433252772 1104 90-91 -0.4596161432487662 1104 92-93 -0.8214734810935802 1104 94-95 -0.9161893296463539 1104 96-97 -1.1649404811955648 1104 98-99 -0.9416201593049784 1104 100-101 -0.8593817214281358 1105 1 0.2903099479037081 1105 2 0.3226277532818713 1105 3 0.2794763937456892 1105 4 0.13567777520930946 1105 5 0.2605743585562976 1105 6 0.3030508808229442 1105 7 0.324195764928902 1105 8 0.32867728255124007 1105 9 0.4675634493216876 1105 10-11 0.39207350081007775 1105 12-13 0.3546497978583574 1105 14-15 0.44359962563907374 1105 16-17 0.36641985604214256 1105 18-19 0.43612062121962225 1105 20-21 0.3931886172091552 1105 22-23 0.5104693009113532 1105 24-25 0.3177658592113062 1105 26-27 0.5088735848503845 1105 28-29 0.5176988860867269 1105 30-31 0.3803250634740891 1105 32-33 0.332865912005893 1105 34-35 0.24974499950991458 1105 36-37 0.441550823195314 1105 38-39 0.250309996290774 1105 40-41 0.3666205808409373 1105 42-43 0.5008128889582153 1105 44-45 0.20473524620603456 1105 46-47 0.5858317400847852 1105 48-49 0.5731885792441602 1105 50-51 0.40304959472393165 1105 52-53 0.7660149485211676 1105 54-55 0.6551170237172457 1105 56-57 0.5503522584220448 1105 58-59 0.458604419318867 1105 60-61 0.5071596030957792 1105 62-63 0.6458316435642502 1105 64-65 0.5498459334051553 1105 66-67 0.30388108415344206 1105 68-69 0.2182587583579938 1105 70-71 0.18106940449482423 1105 72-73 0.22305643932843466 1105 74-75 0.011559099743639223 1105 76-77 0.0536825204001552 1105 78-79 -0.11089247535149127 1105 80-81 -0.3774046416740262 1105 82-83 -0.5329668396568188 1105 84-85 -0.3437161897149217 1105 86-87 -0.29023111943310553 1105 88-89 -0.5879363923264265 1105 90-91 -0.6573281016313253 1105 92-93 -0.9454430314512408 1105 94-95 -1.1426019613973892 1105 96-97 -0.9495391051548978 1105 98-99 -1.0682744695726907 1105 100-101 -1.0329593731828108 1106 1 0.2517429723115967 1106 2 0.1955647714555937 1106 3 0.16051226806034435 1106 4 0.22057313019525537 1106 5 0.23359556587031705 1106 6 0.13298881742365154 1106 7 0.15857624418080718 1106 8 -0.027094891894222428 1106 9 0.251656572251747 1106 10-11 0.221606146714727 1106 12-13 0.3105322531198311 1106 14-15 0.11411046586061246 1106 16-17 -0.25764084363223816 1106 18-19 0.16425927586324462 1106 20-21 0.2911127634895294 1106 22-23 0.25985527680959564 1106 24-25 0.16125059384156515 1106 26-27 0.07540561847037708 1106 28-29 0.07727661840701217 1106 30-31 -0.07898235908581341 1106 32-33 0.12546840010809035 1106 34-35 -0.10032179517990869 1106 36-37 -0.03283236959085656 1106 38-39 -0.20813118263550479 1106 40-41 -0.06292493695706014 1106 42-43 -0.20377541918795572 1106 44-45 0.1450548866279533 1106 46-47 -0.007807805154698144 1106 48-49 0.16281522475917853 1106 50-51 0.11958093256590274 1106 52-53 0.14310402202315942 1106 54-55 0.11778331466378233 1106 56-57 -0.18262205345349258 1106 58-59 -0.08584361997866807 1106 60-61 0.22341958751034952 1106 62-63 -0.020198386215046327 1106 64-65 -0.18869286317718093 1106 66-67 -0.2056145463605965 1106 68-69 -0.2906385030597214 1106 70-71 -0.43636574899380776 1106 72-73 -0.5508157045027993 1106 74-75 -0.5882659120845872 1106 76-77 -1.0589813353233168 1106 78-79 -0.8380135546423553 1106 80-81 -0.7627943469424565 1106 82-83 -0.7476195768475442 1106 84-85 -0.5409918708816015 1106 86-87 -0.6122511021778116 1106 88-89 -0.869578637184631 1106 90-91 -1.012209680212493 1106 92-93 -1.0059235357511476 1106 94-95 -1.3284320941518324 1106 96-97 -1.3902083740313564 1106 98-99 -1.8312211590608776 1106 100-101 -1.7391392735473996 1107 1 0.11388986496696418 1107 2 0.037655166935820716 1107 3 0.03212243755186961 1107 4 0.147126802511643 1107 5 0.16989500089856335 1107 6 0.09570068183042935 1107 7 0.06097737412430604 1107 8 0.043526577032331204 1107 9 0.10448143100880714 1107 10-11 0.22061744614975964 1107 12-13 0.043159371763898946 1107 14-15 0.10245792348772653 1107 16-17 0.19899757444686372 1107 18-19 0.13925927586324605 1107 20-21 0.20777943015620082 1107 22-23 0.33118296042542283 1107 24-25 0.09627884242913609 1107 26-27 0.12251013824439383 1107 28-29 0.24747435852000166 1107 30-31 0.25237357311758046 1107 32-33 0.05986105547532361 1107 34-35 0.26366125566755017 1107 36-37 0.17083994679331482 1107 38-39 -0.10071592839820909 1107 40-41 0.07450444157401392 1107 42-43 -0.048125701673832566 1107 44-45 -0.1504253393607442 1107 46-47 0.0016554716814596304 1107 48-49 -0.007100029478110059 1107 50-51 -0.10598403918550048 1107 52-53 -0.21974908532147452 1107 54-55 -0.11053589437575795 1107 56-57 -0.34618137548739014 1107 58-59 -0.3239792131990171 1107 60-61 -0.26577532774388857 1107 62-63 -0.15967578734499455 1107 64-65 -0.2275346710867865 1107 66-67 -0.1568857328012747 1107 68-69 -0.30779952000887434 1107 70-71 -0.2680747885418313 1107 72-73 -0.30462926382483246 1107 74-75 -0.3589580024800689 1107 76-77 -0.3576395274137134 1107 78-79 -0.21188361113953036 1107 80-81 -0.10982824524754164 1107 82-83 -0.28328341865545426 1107 84-85 -0.3739720968703004 1107 86-87 -0.502364096528094 1107 88-89 -0.5457085806874531 1107 90-91 -0.8336079852972418 1107 92-93 -1.0134800329262923 1107 94-95 -1.3241948060162407 1107 96-97 -1.2554626113194907 1107 98-99 -1.146899125162573 1107 100-101 -1.301921759423104 1108 1 -0.18696824068651097 1108 2 -0.2271712702345603 1108 3 -0.11475017447162728 1108 4 -0.029026002930123695 1108 5 -0.04249053281809978 1108 6 -0.11929737647064798 1108 7 -0.10727835388751572 1108 8 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-0.44120984079609826 2316 54-55 -0.3058608698625278 2316 56-57 -0.27901889941697533 2316 58-59 -0.16037407475759125 2316 60-61 -0.26509566906153026 2316 62-63 -0.032552765913418824 2316 64-65 0.10093395196966526 2316 66-67 -0.12152246997268179 2316 68-69 -0.13729507260727658 2316 70-71 -0.19512777215297916 2316 72-73 -0.022189204526146256 2316 74-75 -0.12569383149440227 2316 76-77 -0.13392180317595503 2316 78-79 -0.024551993732799815 2316 80-81 -0.07894506833200765 2316 82-83 -0.13050174988765306 2316 84-85 -0.31930320066635076 2316 86-87 -0.09241580778550684 2316 88-89 -0.07478653278158376 2316 90-91 -0.03270595653504316 2316 92-93 -0.08776710627923379 2316 94-95 -0.14210960500769687 2316 96-97 -0.4734381097553104 2316 98-99 -0.7344682875102393 2316 100-101 -0.4653322510886042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5467.0 3 1805.0 4 1723.0 5 1843.0 6 2279.0 7 2753.0 8 3507.0 9 4548.0 10 5685.0 11 7027.0 12 8998.0 13 11165.0 14 13115.0 15 15980.0 16 19014.0 17 23872.0 18 23498.0 19 19877.0 20 19763.0 21 21130.0 22 23021.0 23 25168.0 24 28285.0 25 31488.0 26 35029.0 27 40873.0 28 46035.0 29 53244.0 30 61235.0 31 72888.0 32 87863.0 33 107733.0 34 136292.0 35 176787.0 36 209733.0 37 211216.0 38 97946.0 39 5234.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36138849950142 18.464853967657557 14.622563116898132 22.551194415942888 2 15.767699506340094 19.82321849895144 40.18816347205462 24.220918522653854 3 19.356105198187485 24.348954491396572 29.530341577972813 26.764598732443133 4 12.768177297432674 16.706702114594094 36.6078115480293 33.91730903994394 5 13.63388274416251 37.30800742186558 33.80123617436565 15.256873659606255 6 31.9661306938328 35.890018133513266 17.180871252055834 14.962979920598105 7 28.644138536617092 33.25660543491288 19.953409181932447 18.145846846537577 8 26.81197441069013 34.253199867143536 19.335489041748698 19.599336680417633 9 26.76523351433353 16.64766365267226 20.081269489627605 36.505833343366604 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26.256881534542707 29.81736004850739 21.676152990862736 36-37 22.250178883179203 25.866056367609296 29.685520662887804 22.198244086323697 38-39 21.74483643380423 26.207408944817185 29.784132627662245 22.263621993716338 40-41 22.181652021385688 26.00994080220102 29.80240226527316 22.00600491114013 42-43 21.897641800752 26.06972708230489 29.6078833062741 22.424747810669007 44-45 21.912617084397006 26.44101328380079 29.65690933282646 21.989460298975743 46-47 22.289994501481715 26.732935643272498 28.81575511981837 22.161314735427425 48-49 22.16930015444515 26.64926213717797 28.79648580894218 22.384951899434704 50-51 22.382165015000652 26.990376360872332 28.485779309025478 22.14167931510154 52-53 22.52305108874876 26.77141012428537 28.49547909666737 22.2100596902985 54-55 22.070895746856763 27.008361908199486 28.653552307044457 22.267190037899294 56-57 22.213758547292564 27.223799680151277 28.371327648871297 22.191114123684862 58-59 22.249968717514342 26.723484483887113 28.41704005120702 22.60950674739152 60-61 22.02931206482808 26.92997459532827 28.71194073706932 22.328772602774333 62-63 21.96546448902688 26.982341819021844 28.792827240028657 22.25936645192262 64-65 21.82533977750175 26.905235235205126 28.645521613728846 22.62390337356427 66-67 21.771978145915654 27.042654923575064 28.56140912287118 22.623957807638103 68-69 22.136141555883725 26.769696730856644 28.66025920064209 22.433902512617536 70-71 22.073400031101965 26.877630619972813 28.331572192198102 22.717397156727124 72-73 22.07328675670172 26.968856301756894 28.267284934504055 22.69057200703733 74-75 21.99621029836381 27.33553897978826 28.07865134744947 22.58959937439846 76-77 22.242947431473997 27.01783116415268 28.117566965346235 22.62165443902709 78-79 22.33643153921262 27.122277041491543 28.035530584671715 22.505760834624127 80-81 22.37019568643619 27.32053055132796 27.796026368185682 22.51324739405017 82-83 22.386237954421205 27.41427288697559 27.702049680438456 22.49743947816475 84-85 22.428697551564373 27.312518342819477 27.70171688790312 22.55706721771303 86-87 22.5731001889677 27.27552116474351 27.470382831003214 22.680995815285574 88-89 22.568583017240627 27.330655382089375 27.36064771403981 22.74011388663019 90-91 22.481350161902295 27.37808572168937 27.363609468613166 22.77695464779517 92-93 22.596106255823813 26.953318335715764 27.50852303755011 22.942052370910318 94-95 22.44363345347767 26.96487132120658 27.586633291012042 23.004861934303715 96-97 22.821691107343938 26.677516933485958 27.425433246039827 23.075358713130274 98-99 22.597715276818967 26.786795566998123 27.082605764779455 23.532883391403455 100-101 23.51836053512954 25.63936613362826 27.28053481647292 23.561738514769285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1833.0 1 1216.0 2 923.5 3 1178.0 4 864.5 5 736.0 6 1403.0 7 2894.0 8 4628.0 9 5288.0 10 4056.5 11 2705.0 12 2430.5 13 2505.5 14 2699.0 15 2796.0 16 2863.0 17 2996.5 18 3171.0 19 3412.0 20 3743.5 21 4272.0 22 4983.5 23 5897.0 24 7126.5 25 8707.5 26 10740.0 27 13127.5 28 15066.5 29 17106.5 30 19730.0 31 22955.0 32 27245.0 33 29407.0 34 30164.5 35 32275.5 36 35281.5 37 38355.0 38 40139.5 39 41697.5 40 43998.0 41 47106.0 42 49349.0 43 50849.0 44 53026.5 45 55918.0 46 57429.0 47 58367.5 48 59688.0 49 59315.0 50 58424.5 51 57161.5 52 56109.5 53 56351.5 54 57177.0 55 54685.0 56 49851.5 57 45654.5 58 42251.0 59 37835.0 60 31567.5 61 26555.0 62 22388.5 63 17601.0 64 13350.0 65 10404.0 66 7952.5 67 6238.5 68 4829.5 69 3520.0 70 2689.0 71 1986.5 72 1508.5 73 1206.0 74 888.5 75 680.0 76 521.5 77 338.5 78 206.5 79 142.5 80 99.0 81 61.0 82 36.5 83 24.5 84 15.0 85 10.0 86 8.0 87 6.0 88 4.5 89 5.0 90 6.5 91 4.5 92 4.0 93 3.5 94 2.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14376602035091895 2 0.05056763827483181 3 0.1469528037380368 4 0.1257276238200634 5 0.18994431546990925 6 0.1929507148917185 7 0.16270633670831733 8 0.07065038641251768 9 0.11899328911521065 10-11 0.10170649243980738 12-13 0.1469227397438187 14-15 0.13486707806236356 16-17 0.13149991070993716 18-19 0.12987645502216016 20-21 0.13035747892964966 22-23 0.19036521138896254 24-25 0.20879443984465332 26-27 0.14295429250703046 28-29 0.12479563999930252 30-31 0.0963250374747688 32-33 0.12013572089549815 34-35 0.09118409446347496 36-37 0.08538174357938307 38-39 0.052401541922135454 40-41 0.05889536467324347 42-43 0.0616011241528718 44-45 0.038662296564467125 46-47 0.051289174136066026 48-49 0.04308170371452674 50-51 0.051589814078246955 52-53 0.027268042755810018 54-55 0.04924482252923573 56-57 0.027538618703772853 58-59 0.05092840620544892 60-61 0.037850568720578626 62-63 0.04990623040203376 64-65 0.038121144668541454 66-67 0.038361656622286196 68-69 0.06298406788690407 70-71 0.050778086234358456 72-73 0.05053757428061371 74-75 0.0432320236856172 76-77 0.021405563883281954 78-79 0.022998955576840865 80-81 0.026065482987086313 82-83 0.02236761169826092 84-85 0.020623900033611545 86-87 0.02411132336291029 88-89 0.06178150811818035 90-91 0.10687749944531932 92-93 0.07813632097282275 94-95 0.07389729778807169 96-97 0.06623097926245806 98-99 0.05438576554052957 100-101 0.12855363927656407 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1663119.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.04760990228268 #Duplication Level Percentage of deduplicated Percentage of total 1 80.56492871544202 47.57166482594633 2 11.58824748506824 13.685166338988358 3 3.3846479248543235 5.995661109701061 4 1.4105172675811934 3.33150693506272 5 0.7739958036444654 2.285130113980109 6 0.4560027258320398 1.6155522641584712 7 0.30539266513005864 1.2622894870332706 8 0.22156686156380062 1.04663948871139 9 0.16647071845322498 0.8846728239040884 >10 1.0110571430661546 11.141581284231412 >50 0.07617172750872088 3.1034287423127354 >100 0.036105659966005885 3.9142417466759483 >500 0.0032635345931869664 1.3942730635288603 >1k 0.0013258109284822051 1.2047608046023572 >5k 2.039709120741854E-4 0.8969162962984858 >10k+ 1.019854560370927E-4 0.6665146748644851 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11068 0.6654965760116984 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9397 0.565022707334833 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5497 0.3305235524337104 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2350 0.14130077282503536 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1846 0.11099626665319799 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1777 0.10684743545110122 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1729 0.10396129200616433 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1687 0.10143591649184455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2025597687237053E-4 0.0 10-11 0.0 0.0 0.0 1.2025597687237053E-4 0.0 12-13 0.0 0.0 0.0 1.2025597687237053E-4 0.0 14-15 0.0 0.0 0.0 1.2025597687237053E-4 0.0 16-17 0.0 0.0 0.0 1.2025597687237053E-4 0.0 18-19 0.0 0.0 0.0 1.803839653085558E-4 0.0 20-21 0.0 6.0127988436185266E-5 0.0 3.0063994218092633E-4 0.0 22-23 0.0 6.0127988436185266E-5 0.0 7.515998554523158E-4 0.0 24-25 0.0 6.0127988436185266E-5 0.0 0.0012927517513779832 0.0 26-27 0.0 6.0127988436185266E-5 0.0 0.002194671577920762 0.0 28-29 0.0 6.0127988436185266E-5 0.0 0.005261198988166211 0.0 30-31 0.0 6.0127988436185266E-5 0.0 0.013799373346104517 0.0 32-33 0.0 6.0127988436185266E-5 0.0 0.030605146114018298 0.0 34-35 0.0 6.0127988436185266E-5 0.0 0.05922606860964248 0.0 36-37 0.0 6.0127988436185266E-5 0.0 0.10299924419118536 0.0 38-39 0.0 6.0127988436185266E-5 0.0 0.16646433598557892 0.0 40-41 0.0 6.0127988436185266E-5 0.0 0.23233454731741987 0.0 42-43 0.0 6.0127988436185266E-5 0.0 0.29721264684006377 0.0 44-45 0.0 6.0127988436185266E-5 0.0 0.3807304227779251 0.0 46-47 0.0 6.0127988436185266E-5 0.0 0.47564245252444354 0.0 48-49 0.0 6.0127988436185266E-5 0.0 0.5730798577852818 0.0 50-51 0.0 6.0127988436185266E-5 0.0 0.6845872123401873 0.0 52-53 0.0 9.01919826542779E-5 0.0 0.7838585212483292 0.0 54-55 0.0 1.2025597687237053E-4 0.0 0.8903752527630313 0.0 56-57 0.0 1.2025597687237053E-4 0.0 1.0178165242535262 0.0 58-59 0.0 1.2025597687237053E-4 0.0 1.1513607865702935 0.0 60-61 0.0 1.2025597687237053E-4 0.0 1.3006886458515596 0.0 62-63 0.0 1.2025597687237053E-4 0.0 1.441358074798015 0.0 64-65 0.0 1.2025597687237053E-4 0.0 1.5849136471894074 0.0 66-67 0.0 1.2025597687237053E-4 0.0 1.7507165753021883 0.0 68-69 0.0 1.2025597687237053E-4 0.0 1.932663868310085 0.0 70-71 0.0 1.2025597687237053E-4 0.0 2.1294327104675013 0.0 72-73 0.0 1.2025597687237053E-4 0.0 2.326081296648045 0.0 74-75 0.0 1.2025597687237053E-4 0.0 2.522068474955791 0.0 76-77 0.0 1.2025597687237053E-4 0.0 2.727375491471146 0.0 78-79 0.0 1.2025597687237053E-4 0.0 2.9610328545341615 0.0 80-81 0.0 1.2025597687237053E-4 0.0 3.208579782925936 0.0 82-83 0.0 1.2025597687237053E-4 0.0 3.4560064553408383 0.0 84-85 0.0 1.2025597687237053E-4 0.0 3.7046657515186827 0.0 86-87 0.0 1.2025597687237053E-4 0.0 3.9673949969905946 0.0 88-89 0.0 1.2025597687237053E-4 0.0 4.2441941917565735 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4330 0.0 56.43175 1 GTATCAA 7650 0.0 43.335308 1 ATCAACG 9805 0.0 33.52035 3 TATCAAC 9945 0.0 33.236774 2 TCAACGC 9910 0.0 33.069202 4 CAACGCA 10075 0.0 32.52762 5 AACGCAG 10405 0.0 31.535046 6 TGGTATC 1890 0.0 30.951532 2 GTGGTAT 1955 0.0 29.967632 1 ACGCAGA 12000 0.0 27.144552 7 CGCAGAG 12065 0.0 26.99587 8 GCAGAGT 12930 0.0 24.711082 9 GAGTACT 7855 0.0 24.211504 12-13 GTACATG 7980 0.0 23.815742 1 TACATGG 7830 0.0 23.627983 2 GTACTTT 8335 0.0 22.500072 14-15 CAGAGTA 12495 0.0 22.144966 10-11 TAAGGTG 970 0.0 22.065762 5 ACATGGG 8065 0.0 21.880018 3 AGTACTT 8000 0.0 21.633127 12-13 >>END_MODULE