FastQCFastQC Report
Thu 26 May 2016
SRR937964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663119
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106710.641625764602533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94470.5680291067566422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57720.3470587492536613No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.14827561948363288No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.1138222821096987No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18820.11316087423690066No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17780.10690756343953739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45200.056.034871
GTATCAA79150.042.3860931
GTGGTAT19200.035.391941
TGGTATC19250.033.073362
ATCAACG101800.032.7568553
TATCAAC102800.032.676132
TCAACGC102850.032.414644
CAACGCA104650.031.8571035
AACGCAG107650.030.9251886
TAGCGCG1401.7102138E-830.5297244
ACGCAGA123600.026.8968267
CGCAGAG123750.026.8258468
GCAGAGT132700.024.7660439
GGACCGA4950.023.9851916
GAGTACT85650.023.64972912-13
TACATGG73250.023.4155242
GTACATG76850.022.940331
GGTCGTG1050.002198753622.615298
GTACTTT89900.022.2140514-15
CAGAGTA128300.022.0251210-11
Produced by FastQC (version 0.11.5)