##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937964_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1663119 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.547206784361194 34.0 31.0 34.0 31.0 34.0 2 32.76049037982249 34.0 31.0 34.0 31.0 34.0 3 32.804891291603305 34.0 31.0 34.0 31.0 34.0 4 36.25234273674945 37.0 35.0 37.0 35.0 37.0 5 36.23109651203552 37.0 37.0 37.0 35.0 37.0 6 36.26839630838202 37.0 37.0 37.0 35.0 37.0 7 36.2287172475331 37.0 37.0 37.0 35.0 37.0 8 36.21521791284929 37.0 37.0 37.0 35.0 37.0 9 37.909082272525296 39.0 38.0 39.0 35.0 39.0 10-11 37.89325658596889 39.0 38.0 39.0 35.0 39.0 12-13 37.81369192463077 39.0 38.0 39.0 35.0 39.0 14-15 39.09211277124487 40.0 38.5 41.0 36.0 41.0 16-17 38.980112968464674 40.0 38.0 41.0 36.0 41.0 18-19 39.241218457608866 40.5 39.0 41.0 36.0 41.0 20-21 39.23106103652234 41.0 39.0 41.0 36.0 41.0 22-23 39.13812120479653 40.5 39.0 41.0 35.5 41.0 24-25 39.03603470346981 40.0 39.0 41.0 35.0 41.0 26-27 38.86133403562823 40.0 38.0 41.0 35.0 41.0 28-29 38.741338112305854 40.0 38.0 41.0 35.0 41.0 30-31 38.48551606950555 40.0 38.0 41.0 34.0 41.0 32-33 38.24749191128235 40.0 38.0 41.0 34.0 41.0 34-35 38.07647378209256 40.0 37.5 41.0 34.0 41.0 36-37 38.04952351575564 40.0 38.0 41.0 34.0 41.0 38-39 37.89130362890448 40.0 37.0 41.0 33.0 41.0 40-41 37.70654294731766 40.0 37.0 41.0 33.0 41.0 42-43 37.462244734141095 40.0 37.0 41.0 33.0 41.0 44-45 37.16184770903345 40.0 36.0 41.0 32.0 41.0 46-47 36.71817560860047 40.0 36.0 41.0 31.0 41.0 48-49 36.28108992802079 39.0 35.0 41.0 30.0 41.0 50-51 35.89031843181396 39.0 35.0 41.0 29.0 41.0 52-53 35.38094898801589 38.5 35.0 40.0 27.5 41.0 54-55 35.070041891169545 38.0 34.5 40.0 26.5 41.0 56-57 34.724446957794356 38.0 34.0 40.0 26.0 41.0 58-59 34.28426017621108 37.5 34.0 40.0 24.5 41.0 60-61 33.81445615136379 37.0 33.0 40.0 23.0 41.0 62-63 33.357589264508434 36.5 33.0 39.0 22.0 41.0 64-65 32.8780267677779 36.0 32.0 39.0 21.0 40.0 66-67 32.33448478431188 35.0 31.5 39.0 18.5 40.0 68-69 31.75967564557918 35.0 31.0 38.0 16.5 40.0 70-71 31.15514524216247 35.0 30.5 37.5 10.5 39.5 72-73 30.587707794812037 34.5 30.0 37.0 7.5 39.0 74-75 29.994121887850476 34.0 29.0 36.0 6.5 39.0 76-77 28.635483089303893 32.5 27.0 35.0 2.0 37.0 78-79 29.308805623650503 34.0 29.0 35.0 2.0 37.0 80-81 28.97808875973397 34.0 29.0 35.0 2.0 37.0 82-83 28.42317627301474 34.0 27.5 35.0 2.0 36.5 84-85 27.84434427121571 33.0 26.5 35.0 2.0 36.0 86-87 27.212418954987584 33.0 25.5 35.0 2.0 35.5 88-89 26.47992657170052 32.0 24.5 35.0 2.0 35.0 90-91 25.94495914002546 32.0 23.5 34.5 2.0 35.0 92-93 25.142894164518594 31.5 19.5 34.0 2.0 35.0 94-95 24.331718295563938 31.0 14.0 34.0 2.0 35.0 96-97 23.30535758415363 31.0 2.0 34.0 2.0 35.0 98-99 21.87499782036042 30.0 2.0 34.0 2.0 35.0 100-101 17.50493229889142 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1345719668567682 1101 2 0.10812965261509788 1101 3 0.10731680534219379 1101 4 0.0808752477592165 1101 5 0.11822506583191483 1101 6 0.07620020001276373 1101 7 0.10476520450945515 1101 8 0.10117434744532261 1101 9 0.0876048240322902 1101 10-11 0.06485014638766273 1101 12-13 0.20319826332892887 1101 14-15 0.2124602837731473 1101 16-17 0.2521599001947905 1101 18-19 0.2298025680852973 1101 20-21 0.26704390667335787 1101 22-23 0.23919574454678383 1101 24-25 0.26161473800652857 1101 26-27 0.26471445980089214 1101 28-29 0.29957219312977657 1101 30-31 0.21268365527149768 1101 32-33 0.3879941657701309 1101 34-35 0.36776485312852714 1101 36-37 0.4280381954660655 1101 38-39 0.4586747303435317 1101 40-41 0.4999920368026807 1101 42-43 0.5353444197781059 1101 44-45 0.45531036317537144 1101 46-47 0.563044779784029 1101 48-49 0.7182885477886884 1101 50-51 0.6868675706451626 1101 52-53 0.6274955118272345 1101 54-55 0.5512325300456169 1101 56-57 0.6450771540630313 1101 58-59 0.826082394049557 1101 60-61 0.5814950972260462 1101 62-63 0.4486660418465718 1101 64-65 0.6330865350747175 1101 66-67 0.386655539260623 1101 68-69 0.4776412245522934 1101 70-71 0.6522764176835558 1101 72-73 0.4163709958666324 1101 74-75 0.3756473988064464 1101 76-77 0.1423603481958331 1101 78-79 0.07740722332225758 1101 80-81 0.014009547121951016 1101 82-83 0.11506510397887837 1101 84-85 0.1799993479624824 1101 86-87 -0.21383339446090588 1101 88-89 -0.37865864549318573 1101 90-91 -0.22116306614500658 1101 92-93 -0.22032001464065587 1101 94-95 -0.27550820977639745 1101 96-97 -0.410078427855165 1101 98-99 -0.5054706157436115 1101 100-101 -0.5468352109073393 1102 1 0.10917538357427503 1102 2 0.1257444105377914 1102 3 0.07812666070594076 1102 4 0.007074867326387846 1102 5 0.04918727377793175 1102 6 -0.010289948212481193 1102 7 0.060497717160636455 1102 8 0.06221946103682541 1102 9 0.0929874102577557 1102 10-11 0.1365182187416778 1102 12-13 0.09727643150895204 1102 14-15 0.14497248633568205 1102 16-17 0.1259719448418153 1102 18-19 0.17536612908016025 1102 20-21 0.09493932353442602 1102 22-23 0.11289249292276082 1102 24-25 0.19475097602827418 1102 26-27 0.11594009953817164 1102 28-29 0.07092308858253205 1102 30-31 0.058457620745421934 1102 32-33 0.04664138609815893 1102 34-35 0.1910986649937243 1102 36-37 0.3262903578319225 1102 38-39 0.1842718483853787 1102 40-41 0.25147608374665253 1102 42-43 0.20989086129552703 1102 44-45 0.11490507659460292 1102 46-47 0.12223304020107406 1102 48-49 0.13106848892926592 1102 50-51 0.07443217244683353 1102 52-53 0.10806876309235491 1102 54-55 0.27024744229483844 1102 56-57 0.16136578055694883 1102 58-59 0.02959242527375494 1102 60-61 0.08787542238844281 1102 62-63 0.157075043030936 1102 64-65 0.17652317147424412 1102 66-67 0.0030258152538706895 1102 68-69 0.04075808203354114 1102 70-71 0.0049726456561351995 1102 72-73 0.12792843763528694 1102 74-75 -0.016706708791442537 1102 76-77 -0.10769662700425542 1102 78-79 -0.2964097561846195 1102 80-81 -0.379870778040452 1102 82-83 -0.37857431029812005 1102 84-85 -0.4374830659915112 1102 86-87 -0.7203361938723098 1102 88-89 -0.749444723804924 1102 90-91 -0.8330444141692723 1102 92-93 -0.5286796672265197 1102 94-95 -0.5977227057028927 1102 96-97 -0.5591403558600483 1102 98-99 -0.5538842826136516 1102 100-101 -0.8384840821703037 1103 1 0.15417012232448712 1103 2 0.16133433369701322 1103 3 0.12563618843140034 1103 4 0.14380634905349154 1103 5 0.06629915712851187 1103 6 0.07764366277336165 1103 7 0.024735366487284693 1103 8 0.012125289086796442 1103 9 0.15738510768725433 1103 10-11 0.15463062379601666 1103 12-13 0.2149590941434667 1103 14-15 0.2601381493857886 1103 16-17 0.20510688926709264 1103 18-19 0.16847477609893957 1103 20-21 0.2500904849884691 1103 22-23 0.2487720058813494 1103 24-25 0.19384948961467785 1103 26-27 0.2175936351864607 1103 28-29 0.35283500022890735 1103 30-31 0.3197905296418 1103 32-33 0.14244564992110043 1103 34-35 0.1842028907941824 1103 36-37 0.1980529207237609 1103 38-39 0.1386183481068457 1103 40-41 0.329740932408356 1103 42-43 0.09610063926194101 1103 44-45 0.026509890417095505 1103 46-47 0.005601743280891469 1103 48-49 0.08775204464988207 1103 50-51 0.08395061334840648 1103 52-53 0.013069603123831541 1103 54-55 0.07260837287286392 1103 56-57 0.10467957210535417 1103 58-59 0.18861436335901516 1103 60-61 0.002317774122893468 1103 62-63 0.09165604417160722 1103 64-65 0.10290944447562822 1103 66-67 0.00549418143685898 1103 68-69 0.12904981170256846 1103 70-71 -0.1605674945849067 1103 72-73 -0.23962954664285974 1103 74-75 -0.1413302420522662 1103 76-77 0.005323418799566326 1103 78-79 0.013037503102150794 1103 80-81 -0.019543696309810343 1103 82-83 -0.13180577876156718 1103 84-85 -0.29572819601954237 1103 86-87 -0.3054932797588954 1103 88-89 -0.49642680018589047 1103 90-91 -0.8378219826760471 1103 92-93 -0.670031322088537 1103 94-95 -0.8936251205723345 1103 96-97 -1.0666166360238094 1103 98-99 -0.9484877435433461 1103 100-101 -1.7096316535950855 1104 1 0.1558547698396353 1104 2 0.050955271506133215 1104 3 0.15572265655386275 1104 4 0.11575515292900462 1104 5 -0.027621591838382642 1104 6 0.11136012554158015 1104 7 0.058200204626650986 1104 8 0.09369374377332917 1104 9 0.07612231504987932 1104 10-11 0.11595344334485702 1104 12-13 0.15656671152223112 1104 14-15 0.24395043576267028 1104 16-17 0.15954096897682746 1104 18-19 0.07943100743849385 1104 20-21 0.1680581747361316 1104 22-23 0.10872990619345302 1104 24-25 0.14763305778392066 1104 26-27 0.10948105090252369 1104 28-29 0.08055776433782569 1104 30-31 0.08603743692761157 1104 32-33 0.12444582647084701 1104 34-35 0.14103709821372945 1104 36-37 0.10196139370875557 1104 38-39 0.07982790357274894 1104 40-41 0.13719364141560675 1104 42-43 0.1826524990979479 1104 44-45 0.22055682294787005 1104 46-47 0.19801229072533744 1104 48-49 0.20939130898197078 1104 50-51 0.2216622977236753 1104 52-53 0.12410701434365734 1104 54-55 0.16550364835233466 1104 56-57 0.11050447601361668 1104 58-59 0.18407398255607887 1104 60-61 -0.023198613240438704 1104 62-63 0.07693043464188065 1104 64-65 0.27822539838459903 1104 66-67 0.08112821362666267 1104 68-69 0.1792738813720618 1104 70-71 -0.09534989703086438 1104 72-73 -0.2327919567283061 1104 74-75 -0.2334770373857431 1104 76-77 -0.43042464472204145 1104 78-79 -0.35922216031484666 1104 80-81 -0.32317996094631596 1104 82-83 -0.28351604248893025 1104 84-85 -0.44247732956456787 1104 86-87 -0.7205536737948144 1104 88-89 -0.7013369041934929 1104 90-91 -0.6749802166176515 1104 92-93 -0.872632254502161 1104 94-95 -0.9615800640887926 1104 96-97 -1.1400586464468425 1104 98-99 -1.4188735872242262 1104 100-101 -1.89997248534236 1105 1 0.20678423734489826 1105 2 0.19500499836468066 1105 3 0.12611241135889628 1105 4 0.06150009958082592 1105 5 0.08219137335805726 1105 6 0.10733741979095868 1105 7 0.08349008003960279 1105 8 0.09763123741134194 1105 9 0.1298290805060418 1105 10-11 0.16896193216779665 1105 12-13 0.1787126069974292 1105 14-15 0.2728331006114786 1105 16-17 0.21363022500182893 1105 18-19 0.2149336894993752 1105 20-21 0.17322064068984133 1105 22-23 0.18270520162113968 1105 24-25 0.20468295275324522 1105 26-27 0.16242032898241376 1105 28-29 0.017137589194163638 1105 30-31 0.08911933926295745 1105 32-33 0.08296564966148878 1105 34-35 0.18486002975717497 1105 36-37 0.19387846574345247 1105 38-39 0.08697087638815049 1105 40-41 0.08260313766935923 1105 42-43 0.17784264163737618 1105 44-45 0.08801728606992043 1105 46-47 0.09748802416642377 1105 48-49 0.1195210627382437 1105 50-51 0.11540607068467068 1105 52-53 -0.01853594150644966 1105 54-55 0.08925345942049745 1105 56-57 0.10756003122644131 1105 58-59 0.008303950383684366 1105 60-61 -0.10352703074740788 1105 62-63 -0.07214314389669596 1105 64-65 0.05075532672930905 1105 66-67 -0.1701116033330905 1105 68-69 -0.09436688903797119 1105 70-71 -0.06849786381587819 1105 72-73 -0.09853410410438812 1105 74-75 -0.17055791717574564 1105 76-77 -0.15034631127046438 1105 78-79 -0.21237373608345322 1105 80-81 -0.021103792884897388 1105 82-83 -0.2424975919458845 1105 84-85 -0.15443763999885007 1105 86-87 -0.2681012614823217 1105 88-89 -0.5140936972568362 1105 90-91 -0.6819583560478009 1105 92-93 -0.513079359758013 1105 94-95 -0.6829642172577586 1105 96-97 -0.9749295250338506 1105 98-99 -0.7352063972035303 1105 100-101 -1.4085683053990543 1106 1 0.1772658548896402 1106 2 0.050182282603920214 1106 3 0.1436321018768325 1106 4 0.133958422477491 1106 5 0.1347852338128206 1106 6 0.07153616182430511 1106 7 0.1359934893518968 1106 8 0.10053611064058288 1106 9 0.09273715431417173 1106 10-11 0.05470822367096417 1106 12-13 -0.004885817761135058 1106 14-15 0.10858252311572159 1106 16-17 0.16192869985641067 1106 18-19 0.18780356509427065 1106 20-21 0.2574080415414741 1106 22-23 0.3732179204433308 1106 24-25 0.26689246543031686 1106 26-27 0.21458454651045855 1106 28-29 0.22730603705255703 1106 30-31 0.2822760145572758 1106 32-33 0.30910394250054196 1106 34-35 0.31967049982480233 1106 36-37 0.3656156147234455 1106 38-39 0.27755143356719714 1106 40-41 0.3419056897831325 1106 42-43 0.2976956237419657 1106 44-45 0.3339226324282194 1106 46-47 0.4310109570099243 1106 48-49 0.2946031645444762 1106 50-51 0.22520332096994622 1106 52-53 0.11458726686515064 1106 54-55 0.02517056442732013 1106 56-57 -0.02034136311270629 1106 58-59 0.14157153269422196 1106 60-61 -0.02378565143705913 1106 62-63 0.2845126686328783 1106 64-65 0.20825685744095068 1106 66-67 0.00957654895879756 1106 68-69 -0.004287987810617011 1106 70-71 -0.5571879921173455 1106 72-73 -0.34673551792532464 1106 74-75 -0.2748480844407801 1106 76-77 -0.4229399629918156 1106 78-79 -0.43883382625628187 1106 80-81 -0.481698404779916 1106 82-83 -0.46871763870216654 1106 84-85 -0.5533932771923595 1106 86-87 -0.40758735983750327 1106 88-89 -0.5836610624846337 1106 90-91 -0.8334814141313522 1106 92-93 -0.7273198484724936 1106 94-95 -1.0230326818148257 1106 96-97 -1.3082872105978502 1106 98-99 -1.3072489927338538 1106 100-101 -1.7630011071579812 1107 1 0.10071218257325398 1107 2 0.03718793232143014 1107 3 0.05281289283728796 1107 4 0.07011661456788687 1107 5 0.10385303042298943 1107 6 0.05345706577910647 1107 7 0.02554151195076315 1107 8 0.08627057391741744 1107 9 -0.006697873934413678 1107 10-11 0.09665737621333648 1107 12-13 0.07519892800156924 1107 14-15 0.03633676040386291 1107 16-17 0.08120836087335448 1107 18-19 0.035473056619693466 1107 20-21 0.033184877699667936 1107 22-23 0.03670662100829958 1107 24-25 0.10170884961110715 1107 26-27 0.14368059170819691 1107 28-29 -0.008499047693206307 1107 30-31 0.029098048455580283 1107 32-33 0.16235253007116768 1107 34-35 0.13365355067225693 1107 36-37 0.12310149042965435 1107 38-39 0.1065768572960053 1107 40-41 -0.06685137236375738 1107 42-43 0.14020974803575115 1107 44-45 -0.00336550316499995 1107 46-47 -0.11136192434600645 1107 48-49 -0.08449288065325788 1107 50-51 0.10634738876655092 1107 52-53 0.05519461149791738 1107 54-55 0.10631463222392767 1107 56-57 0.11842982332796481 1107 58-59 -0.03879569894419177 1107 60-61 -0.028093561041579562 1107 62-63 -0.045783941536619466 1107 64-65 -0.25678551544040573 1107 66-67 -0.17015508945927849 1107 68-69 -0.2788642589970607 1107 70-71 -0.43084618420774135 1107 72-73 -0.45316207159763877 1107 74-75 -0.44087915788710674 1107 76-77 -0.5751292285285388 1107 78-79 -0.4925061426404618 1107 80-81 -0.3545797607121237 1107 82-83 -0.3811470172332392 1107 84-85 -0.6079130512036564 1107 86-87 -0.6656382072951281 1107 88-89 -0.7054830963829382 1107 90-91 -0.9404023180861536 1107 92-93 -0.8946221083594992 1107 94-95 -0.7916767496114332 1107 96-97 -0.6862533122927594 1107 98-99 -1.1536472978186012 1107 100-101 -1.3051621241071345 1108 1 -0.10960763403015505 1108 2 -0.13427285162384806 1108 3 -0.13862358855372037 1108 4 -0.013685858279650631 1108 5 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154.0 8 420.0 9 737.0 10 1323.0 11 2260.0 12 3416.0 13 5369.0 14 7246.0 15 10000.0 16 13404.0 17 19058.0 18 19602.0 19 14998.0 20 13389.0 21 13622.0 22 14825.0 23 15600.0 24 17836.0 25 20151.0 26 23245.0 27 27426.0 28 32482.0 29 39032.0 30 47441.0 31 57498.0 32 71657.0 33 92785.0 34 125775.0 35 182586.0 36 255258.0 37 321846.0 38 182685.0 39 9893.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.600711018746026 18.988902960132677 14.938309021351007 22.47207699977029 2 16.079336481262764 20.096494540836083 39.627287881594235 24.196881096306917 3 19.528478203873103 24.076824642383805 29.843663933381283 26.551033220361813 4 13.032982005496901 16.922240681514673 36.333419316356796 33.711357996631634 5 13.579747328647732 37.40265971743283 33.951412685518505 15.066180268400927 6 32.20140557467657 35.98045087102818 16.90760342783201 14.910540126463237 7 28.717728557006446 33.20826711738607 19.81698242879794 18.25702189680955 8 26.877511470916993 34.5097374270873 19.165435546103435 19.44731555589227 9 26.55967492404332 16.60704976613219 20.0410794416996 36.792195868124885 10-11 25.207787296038347 26.747905591842798 26.31549516300397 21.728811949114885 12-13 26.29339799935001 24.052956224469888 27.153353402166243 22.50029237401386 14-15 22.9428561636299 25.354259075868896 26.014704900851953 25.688179859649253 16-17 21.997894857734536 28.627108712468896 26.842427906694272 22.5325685231023 18-19 21.84161205542117 26.575939544915308 29.91141944743581 21.67102895222771 20-21 22.45455676953964 25.60935206680941 30.4200421016175 21.516049062033442 22-23 22.288212689530937 25.557070780864148 30.280966064364605 21.87375046524031 24-25 22.035581440935914 25.836771778254274 30.35790092708733 21.76974585372248 26-27 21.475003548240434 26.33476986954503 30.193691829905774 21.99653475230876 28-29 21.62514176034174 26.554944083587788 30.13193483173363 21.687979324336837 30-31 21.63923238117572 26.10691704774017 30.169897147736258 22.083953423347854 32-33 21.370238690075695 26.14536301972378 30.10289702661084 22.381501263589676 34-35 22.200654313426412 26.343556943037573 29.779409001214397 21.67637974232162 36-37 22.29772368209416 25.890947791852874 29.708301202120737 22.103027323932228 38-39 21.675157502626924 26.200259460304526 29.826931258051765 22.297651779016785 40-41 22.176336782558135 26.037879138633823 29.799275921348293 21.986508157459745 42-43 21.86469423299463 26.05397113926745 29.627093022451746 22.454241605286175 44-45 21.898957259797562 26.47448948300722 29.624356179176704 22.00219707801852 46-47 22.299269743824087 26.73738737249078 28.81301052542969 22.150332358255444 48-49 22.171063094687277 26.580659204148372 28.893462645168412 22.354815055995942 50-51 22.368963808708326 26.9974721175285 28.540869437636346 22.09269463612683 52-53 22.58270810294969 26.772638631570782 28.492225134652283 22.15242813082724 54-55 22.082993462759255 26.97899381874017 28.64173136609781 22.296281352402765 56-57 22.23199788316515 27.196967874625642 28.355513993962212 22.215520248246996 58-59 22.219412287227613 26.75199112795602 28.4145972547904 22.613999330025962 60-61 22.051722852387414 27.003991447070934 28.6024643788303 22.341821321711354 62-63 21.959171154592116 27.074534142813754 28.683241169236133 22.28305353335799 64-65 21.798628624833 26.93721100792737 28.571639061850206 22.692521305389427 66-67 21.737458412699368 27.0752758329863 28.45435807849758 22.732907675816747 68-69 22.05296025665161 26.85053255569596 28.55198019057609 22.544526997076343 70-71 21.967247521072796 26.982292324729617 28.244390033715877 22.80607012048171 72-73 22.020609878009363 27.071247641739888 28.13147628962065 22.776666190630095 74-75 21.93628718575377 27.434378435329805 27.896411501093294 22.732922877823132 76-77 21.972279580931108 27.068048327456136 28.267033281281996 22.692638810330763 78-79 22.09907702245091 27.15947066141462 28.22191876967601 22.51953354645846 80-81 22.10985396302321 27.41457122497271 27.92893288071993 22.546641931284146 82-83 22.049785453158776 27.495678387849203 27.867902421185462 22.586633737806558 84-85 22.139676590585427 27.429118875331977 27.821112062661175 22.61009247142142 86-87 22.17746082592595 27.4459399883198 27.633893746282272 22.742705439471973 88-89 22.123381012865295 27.577719802161216 27.454371265263706 22.844527919709783 90-91 22.038548190952294 27.530946690258844 27.619026425724936 22.811478693063926 92-93 22.02248569534974 27.186563672382913 27.756524448111737 23.034426184155606 94-95 21.877558629544268 27.144934007465977 27.77754224228784 23.199965120701915 96-97 22.098466713954487 26.953336302102425 27.676394948121214 23.271802035821878 98-99 21.805281274279405 27.09169707480522 27.409178487154012 23.693843163761358 100-101 22.272310706421013 26.533964422176066 27.488355003710286 23.70536986769264 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1846.0 1 1199.0 2 903.5 3 1210.5 4 893.5 5 762.5 6 1441.0 7 2942.5 8 4585.0 9 5249.5 10 4150.0 11 2669.0 12 2321.0 13 2457.0 14 2607.5 15 2715.0 16 2819.0 17 2943.5 18 3091.5 19 3276.0 20 3597.0 21 4103.0 22 4772.0 23 5651.0 24 6905.0 25 8498.0 26 10500.0 27 12922.5 28 14945.0 29 17068.0 30 19586.5 31 22743.5 32 27366.5 33 29642.0 34 30322.0 35 32196.5 36 35155.5 37 38301.0 38 40126.5 39 41844.5 40 44164.5 41 47374.5 42 49589.0 43 51511.5 44 54109.0 45 56816.5 46 58084.0 47 59627.5 48 61003.5 49 59865.0 50 59191.5 51 57787.5 52 56374.0 53 57306.0 54 58725.0 55 55598.5 56 49844.0 57 45446.0 58 42200.5 59 37753.5 60 31019.5 61 25677.5 62 21718.5 63 16692.5 64 12093.5 65 9278.0 66 7005.5 67 5475.5 68 4218.0 69 3151.5 70 2408.0 71 1706.5 72 1294.5 73 1060.5 74 814.0 75 652.5 76 466.0 77 240.0 78 139.0 79 94.5 80 58.0 81 34.5 82 18.0 83 8.0 84 5.5 85 3.0 86 2.5 87 1.5 88 1.5 89 1.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009199582230736345 2 0.013649053375014054 3 0.009800862115098199 4 0.0 5 6.012798843618527E-4 6 0.0016234556877770022 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.7121589014376E-4 14-15 0.0 16-17 0.002916207439154985 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0036678072946073016 26-27 0.01942134026488784 28-29 0.006072926832054712 30-31 0.0032469113755540045 32-33 0.0 34-35 0.009289774213390624 36-37 9.921118091970567E-4 38-39 0.003096591404463541 40-41 0.0024051195374474106 42-43 0.0038181272656977643 44-45 6.0127988436185266E-5 46-47 2.4051195374474106E-4 48-49 4.5095991327138947E-4 50-51 0.004028575225224413 52-53 0.007936894473576454 54-55 0.006523886745326101 56-57 0.015573149004971984 58-59 0.022127099744516175 60-61 0.0037880632714796716 62-63 0.019481468253324027 64-65 0.020172940120340155 66-67 0.01211578966989133 68-69 0.028620922495624184 70-71 0.01518231708013678 72-73 0.004900431057549099 74-75 0.015092125097482501 76-77 0.02029319609721253 78-79 0.02420151534556457 80-81 0.011063549872258088 82-83 0.03276975369772096 84-85 0.020142876126122065 86-87 0.028620922495624184 88-89 0.021104923941101028 90-91 0.025313883131633998 92-93 0.022427739686697104 94-95 0.014520909207338742 96-97 0.01518231708013678 98-99 0.011243933837566646 100-101 0.010402141999460051 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1663119.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.82333853962594 #Duplication Level Percentage of deduplicated Percentage of total 1 78.87274531916029 44.81812708820341 2 12.413580007894854 14.107621185546835 3 3.795531712970067 6.470243503919533 4 1.584917352628296 3.6024118114290142 5 0.8256272810359057 2.345744924892707 6 0.5018433389313137 1.7109848365170168 7 0.34372804248821004 1.3672242446709342 8 0.2477401270877585 1.1261936889086124 9 0.17753555289191775 0.9079346542317398 >10 1.1065584316091663 11.756472587373231 >50 0.08496542030604153 3.323323196119022 >100 0.039732862027055754 4.136066472283086 >500 0.0035925910182574377 1.4265994271167992 >1k 0.0015849666257018106 1.346563356563487 >5k 2.113288834269081E-4 0.9137801633539799 >10k+ 1.0566444171345405E-4 0.640708858870512 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10671 0.641625764602533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9447 0.5680291067566422 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5772 0.3470587492536613 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2466 0.14827561948363288 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1893 0.1138222821096987 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1882 0.11316087423690066 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1778 0.10690756343953739 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 6.0127988436185266E-5 0.0 6.0127988436185266E-5 0.0 5 0.0 6.0127988436185266E-5 0.0 6.0127988436185266E-5 0.0 6 6.0127988436185266E-5 6.0127988436185266E-5 0.0 6.0127988436185266E-5 0.0 7 6.0127988436185266E-5 6.0127988436185266E-5 0.0 6.0127988436185266E-5 0.0 8 6.0127988436185266E-5 6.0127988436185266E-5 0.0 6.0127988436185266E-5 0.0 9 6.0127988436185266E-5 6.0127988436185266E-5 0.0 1.2025597687237053E-4 0.0 10-11 6.0127988436185266E-5 6.0127988436185266E-5 0.0 1.2025597687237053E-4 0.0 12-13 6.0127988436185266E-5 1.2025597687237053E-4 0.0 1.2025597687237053E-4 0.0 14-15 6.0127988436185266E-5 1.2025597687237053E-4 0.0 1.2025597687237053E-4 0.0 16-17 6.0127988436185266E-5 1.2025597687237053E-4 0.0 1.2025597687237053E-4 0.0 18-19 6.0127988436185266E-5 1.2025597687237053E-4 0.0 1.5031997109046316E-4 0.0 20-21 6.0127988436185266E-5 1.2025597687237053E-4 0.0 4.2089591905329686E-4 0.0 22-23 6.0127988436185266E-5 1.2025597687237053E-4 0.0 8.718558323246863E-4 0.0 24-25 6.0127988436185266E-5 1.2025597687237053E-4 0.0 0.0014130077282503537 0.0 26-27 6.0127988436185266E-5 1.2025597687237053E-4 0.0 0.0023149275547931325 0.0 28-29 6.0127988436185266E-5 1.2025597687237053E-4 0.0 0.0054415829534747665 0.0 30-31 1.2025597687237053E-4 1.2025597687237053E-4 0.0 0.014039885299849258 0.0 32-33 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.031206425998380152 0.0 34-35 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.060067860447749075 0.0 36-37 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.10387110002351005 0.0 38-39 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.16754663977743023 0.0 40-41 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.23437889892425018 0.0 42-43 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.2996778943659474 0.0 44-45 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.383616566222862 0.0 46-47 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.47931025981905084 0.0 48-49 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.5778900968601766 0.0 50-51 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.6899686673052259 0.0 52-53 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.7901719600341286 0.0 54-55 1.2025597687237053E-4 1.803839653085558E-4 0.0 0.8973200354274108 0.0 56-57 1.2025597687237053E-4 1.803839653085558E-4 0.0 1.025933802692411 0.0 58-59 1.2025597687237053E-4 2.4051195374474106E-4 0.0 1.1603799848357212 0.0 60-61 1.2025597687237053E-4 2.4051195374474106E-4 0.0 1.3110005958683653 0.0 62-63 1.2025597687237053E-4 2.4051195374474106E-4 0.0 1.4532934805025979 0.0 64-65 1.2025597687237053E-4 2.4051195374474106E-4 0.0 1.5980215486684957 0.0 66-67 1.2025597687237053E-4 2.4051195374474106E-4 0.0 1.7660191483591974 0.0 68-69 1.2025597687237053E-4 2.4051195374474106E-4 0.0 1.9498304090086158 0.0 70-71 1.5031997109046316E-4 2.4051195374474106E-4 0.0 2.14978603455315 0.0 72-73 1.803839653085558E-4 2.4051195374474106E-4 0.0 2.3477273724850716 0.0 74-75 1.803839653085558E-4 2.4051195374474106E-4 0.0 2.546480438260882 0.0 76-77 1.803839653085558E-4 2.4051195374474106E-4 0.0 2.7559663499725513 0.0 78-79 1.803839653085558E-4 2.4051195374474106E-4 0.0 2.9936222242665735 0.0 80-81 1.803839653085558E-4 2.4051195374474106E-4 0.0 3.2466107356118235 0.0 82-83 1.803839653085558E-4 2.4051195374474106E-4 0.0 3.50122270264485 0.0 84-85 2.1044795952664843E-4 2.4051195374474106E-4 0.0 3.7578489572904887 0.0 86-87 2.4051195374474106E-4 2.4051195374474106E-4 0.0 4.02878567318394 0.0 88-89 2.4051195374474106E-4 2.4051195374474106E-4 0.0 4.3156262420187605 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4520 0.0 56.03487 1 GTATCAA 7915 0.0 42.386093 1 GTGGTAT 1920 0.0 35.39194 1 TGGTATC 1925 0.0 33.07336 2 ATCAACG 10180 0.0 32.756855 3 TATCAAC 10280 0.0 32.67613 2 TCAACGC 10285 0.0 32.41464 4 CAACGCA 10465 0.0 31.857103 5 AACGCAG 10765 0.0 30.925188 6 TAGCGCG 140 1.7102138E-8 30.529724 4 ACGCAGA 12360 0.0 26.896826 7 CGCAGAG 12375 0.0 26.825846 8 GCAGAGT 13270 0.0 24.766043 9 GGACCGA 495 0.0 23.985191 6 GAGTACT 8565 0.0 23.649729 12-13 TACATGG 7325 0.0 23.415524 2 GTACATG 7685 0.0 22.94033 1 GGTCGTG 105 0.0021987536 22.61529 8 GTACTTT 8990 0.0 22.21405 14-15 CAGAGTA 12830 0.0 22.02512 10-11 >>END_MODULE