##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937963_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 124210 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32847596811851 33.0 31.0 34.0 31.0 34.0 2 32.52799291522422 34.0 31.0 34.0 31.0 34.0 3 32.50952419289912 34.0 31.0 34.0 30.0 34.0 4 36.04585782143145 37.0 35.0 37.0 35.0 37.0 5 36.00772884630867 37.0 35.0 37.0 35.0 37.0 6 36.118839062877385 37.0 36.0 37.0 35.0 37.0 7 36.07072699460591 37.0 35.0 37.0 35.0 37.0 8 36.12376620239916 37.0 36.0 37.0 35.0 37.0 9 37.71292166492231 39.0 37.0 39.0 35.0 39.0 10-11 37.73937686176636 39.0 38.0 39.0 35.0 39.0 12-13 37.66511553015055 39.0 37.0 39.0 35.0 39.0 14-15 38.720272119797116 40.0 38.0 41.0 34.5 41.0 16-17 38.60520086949521 40.0 38.0 41.0 34.5 41.0 18-19 38.83241687464778 40.0 38.0 41.0 35.0 41.0 20-21 38.866186297399565 40.0 38.0 41.0 35.0 41.0 22-23 38.702648740037034 40.0 38.0 41.0 35.0 41.0 24-25 38.51160937122614 40.0 38.0 41.0 35.0 41.0 26-27 38.216697528379356 40.0 37.0 41.0 34.0 41.0 28-29 37.96608163593913 40.0 36.0 41.0 34.0 41.0 30-31 37.4601239835762 39.0 35.5 41.0 33.0 41.0 32-33 36.89264551968441 38.5 35.0 41.0 33.0 41.0 34-35 36.46302632638274 38.0 35.0 41.0 33.0 41.0 36-37 36.17414861927381 38.0 35.0 41.0 31.5 41.0 38-39 35.741763948152325 38.0 35.0 41.0 31.0 41.0 40-41 35.415791804202556 38.0 35.0 40.5 30.0 41.0 42-43 34.911138394654216 38.0 35.0 40.0 27.5 41.0 44-45 34.28013042428145 38.0 35.0 40.0 23.0 41.0 46-47 33.459822075517266 38.0 34.0 40.0 8.5 41.0 48-49 32.644895741083644 37.0 33.0 40.0 2.0 41.0 50-51 31.957837533209887 37.0 33.0 40.0 2.0 41.0 52-53 31.339392963529505 36.0 32.0 40.0 2.0 41.0 54-55 30.818170839706948 35.5 31.0 40.0 2.0 41.0 56-57 30.261086063923997 35.0 30.5 39.5 2.0 41.0 58-59 29.509862329925127 35.0 28.5 39.0 2.0 41.0 60-61 28.702399162708318 35.0 26.0 39.0 2.0 40.0 62-63 27.950937927703084 35.0 22.5 38.5 2.0 40.0 64-65 27.26202801706787 35.0 19.0 38.0 2.0 40.0 66-67 26.49187263505354 34.0 5.0 37.5 2.0 40.0 68-69 25.796642782384673 34.0 2.0 37.0 2.0 39.0 70-71 25.21174221077208 33.5 2.0 36.0 2.0 39.0 72-73 24.676531680218986 33.0 2.0 35.5 2.0 38.5 74-75 24.163738829401822 33.0 2.0 35.0 2.0 37.5 76-77 23.009004911037756 30.5 2.0 34.5 2.0 36.0 78-79 23.538809274615573 32.0 2.0 35.0 2.0 36.0 80-81 23.3244062474841 32.0 2.0 35.0 2.0 36.0 82-83 22.94584574510909 32.0 2.0 35.0 2.0 36.0 84-85 22.490564366798164 31.0 2.0 35.0 2.0 35.0 86-87 22.009504065695193 31.0 2.0 35.0 2.0 35.0 88-89 21.467881007970373 30.0 2.0 34.0 2.0 35.0 90-91 21.025867482489332 30.0 2.0 34.0 2.0 35.0 92-93 20.3679293132598 29.0 2.0 34.0 2.0 35.0 94-95 19.769189276225745 28.5 2.0 34.0 2.0 35.0 96-97 19.048148297238548 26.5 2.0 34.0 2.0 35.0 98-99 18.051420980597374 24.5 2.0 34.0 2.0 35.0 100-101 14.974060059576523 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5062832231974639 1101 2 0.47004940297138376 1101 3 0.5837030867388151 1101 4 0.2700585847959971 1101 5 0.03929361768633299 1101 6 0.23915265762493476 1101 7 0.4537096387580064 1101 8 0.4740741219936453 1101 9 0.4438750172239594 1101 10-11 0.6440892016640163 1101 12-13 0.5443308600040169 1101 14-15 0.5971100942831384 1101 16-17 0.7621350366602044 1101 18-19 0.8582345625911998 1101 20-21 0.739595822628786 1101 22-23 0.8233371196315673 1101 24-25 0.8106496457562926 1101 26-27 0.6929014624227037 1101 28-29 0.7923017319779646 1101 30-31 1.13923211636385 1101 32-33 1.1894169972294009 1101 34-35 1.169726155379891 1101 36-37 1.5405199606805482 1101 38-39 0.9777669456362119 1101 40-41 1.4197569226666786 1101 42-43 2.1201991489373526 1101 44-45 2.179506243688408 1101 46-47 1.935022468472404 1101 48-49 2.116790951760599 1101 50-51 1.495902262072164 1101 52-53 2.356439962328537 1101 54-55 2.2728579311719663 1101 56-57 2.1060899346867465 1101 58-59 1.83876964028574 1101 60-61 1.892099884377334 1101 62-63 1.8736069621384566 1101 64-65 2.4556500613571153 1101 66-67 2.561874539309052 1101 68-69 2.6510964896327174 1101 70-71 2.79207593732842 1101 72-73 2.9953114897778548 1101 74-75 3.3498728546239676 1101 76-77 2.986111697971989 1101 78-79 3.049147133079437 1101 80-81 2.542271537779552 1101 82-83 2.4098150428537153 1101 84-85 2.468194413677743 1101 86-87 2.68266246777349 1101 88-89 3.1797231087860887 1101 90-91 3.290253835480712 1101 92-93 2.8244056114981433 1101 94-95 2.9059319018308116 1101 96-97 3.561386522375571 1101 98-99 3.5208009503869775 1101 100-101 3.133540760570403 1102 1 0.29411612602100945 1102 2 0.33893690631154527 1102 3 0.3204092415441906 1102 4 0.2713055786501002 1102 5 0.3750021774084331 1102 6 0.3650990368892053 1102 7 0.10214644289089847 1102 8 0.23046496549591922 1102 9 0.5341039296671752 1102 10-11 0.2635779341838358 1102 12-13 0.22336354905718991 1102 14-15 0.3854774230441578 1102 16-17 0.4140010738918747 1102 18-19 0.5493809062270714 1102 20-21 0.3265291084575921 1102 22-23 0.16902163018647798 1102 24-25 0.37803184937257583 1102 26-27 0.48429720482940297 1102 28-29 0.5447289164439795 1102 30-31 0.4881677180383903 1102 32-33 1.075317059579092 1102 34-35 1.1913704058475147 1102 36-37 1.2481444373884827 1102 38-39 1.6425037408620042 1102 40-41 2.3900072121473883 1102 42-43 2.5133584969377125 1102 44-45 2.398576342557206 1102 46-47 2.7779457783503787 1102 48-49 3.209781064880751 1102 50-51 3.3038206730457134 1102 52-53 2.4362030334962554 1102 54-55 2.1617864071673374 1102 56-57 2.477337819250746 1102 58-59 2.877292843278525 1102 60-61 3.6377131381405867 1102 62-63 3.165002704545156 1102 64-65 3.470391310132392 1102 66-67 3.7122709052126766 1102 68-69 2.9088679334734557 1102 70-71 2.2268759729568153 1102 72-73 2.4051271128294296 1102 74-75 2.428210789958431 1102 76-77 2.6054668240074363 1102 78-79 2.379600504416395 1102 80-81 2.474043159762271 1102 82-83 1.8386027866855486 1102 84-85 2.220309849680234 1102 86-87 2.490224594788721 1102 88-89 2.676115733708155 1102 90-91 2.7492811802745116 1102 92-93 2.4722189187039874 1102 94-95 3.095831251612587 1102 96-97 3.586905360889869 1102 98-99 4.259733879931826 1102 100-101 4.084329038828175 1103 1 0.27260394633103857 1103 2 0.3180574377889229 1103 3 0.19133616340436532 1103 4 0.3300686283179246 1103 5 0.22995314229296326 1103 6 0.42947739816728614 1103 7 0.14169089085040554 1103 8 0.1663238709056003 1103 9 0.2065968116665431 1103 10-11 0.44267473899244436 1103 12-13 0.35710287205623814 1103 14-15 0.6015878311428651 1103 16-17 0.4491956325758366 1103 18-19 0.37957704668895786 1103 20-21 0.40221718185208744 1103 22-23 0.2958011303446568 1103 24-25 0.4040125516819728 1103 26-27 0.3615907828110565 1103 28-29 0.48869443368536025 1103 30-31 0.4150104893133033 1103 32-33 0.5303945666970904 1103 34-35 0.3732748664612444 1103 36-37 -0.01091124119424336 1103 38-39 0.5308302198243595 1103 40-41 0.5114877562789957 1103 42-43 0.9321342008288909 1103 44-45 0.587440626011805 1103 46-47 0.30847409217511057 1103 48-49 0.5949044435583914 1103 50-51 0.3886039694708927 1103 52-53 0.6330552954386803 1103 54-55 0.9590815669269084 1103 56-57 1.123415010013165 1103 58-59 1.2138951843098482 1103 60-61 1.559533764524641 1103 62-63 1.6802273170095674 1103 64-65 2.036667804912522 1103 66-67 2.3860292095467486 1103 68-69 2.134231742394686 1103 70-71 1.892092676531636 1103 72-73 2.0983412855595738 1103 74-75 2.3448510936597913 1103 76-77 2.0926148784205942 1103 78-79 2.33973970087321 1103 80-81 1.8315249693162095 1103 82-83 1.7238843431550208 1103 84-85 2.2508777079529345 1103 86-87 2.59762731544674 1103 88-89 2.5193615419966697 1103 90-91 2.2944804842922295 1103 92-93 2.292726669415785 1103 94-95 2.3801953768894037 1103 96-97 3.0249787553852805 1103 98-99 3.2048066417160683 1103 100-101 3.223723217885434 1104 1 0.1561877954321318 1104 2 0.045437337582619364 1104 3 0.17659994177159177 1104 4 0.0875841013506431 1104 5 -0.4132829319775979 1104 6 -0.15658213616811167 1104 7 0.019969700163699144 1104 8 -0.13525290480910712 1104 9 0.30098231122445895 1104 10-11 0.026339637312510433 1104 12-13 0.2796640273760147 1104 14-15 -0.04669539903691344 1104 16-17 0.12359881559970631 1104 18-19 0.15123929248913015 1104 20-21 0.1735847032196105 1104 22-23 0.3084005998406738 1104 24-25 0.19501608837516216 1104 26-27 0.07847112469139716 1104 28-29 0.023361395076456404 1104 30-31 -0.023907872018853027 1104 32-33 0.18497753162488095 1104 34-35 0.32361379955879954 1104 36-37 0.3337321422422477 1104 38-39 0.5239041956569608 1104 40-41 0.09297906190823113 1104 42-43 0.019004227354081138 1104 44-45 0.69133098852285 1104 46-47 0.036590803050444265 1104 48-49 0.0411540751528463 1104 50-51 -0.5897337847289279 1104 52-53 -0.4188898858168493 1104 54-55 -0.3047704814078962 1104 56-57 0.09828975212928626 1104 58-59 0.00228304166321891 1104 60-61 0.18954407987978428 1104 62-63 0.5241338893644141 1104 64-65 0.3794323200908316 1104 66-67 0.8816820241650802 1104 68-69 0.34249139461985223 1104 70-71 0.22726803756906477 1104 72-73 0.8249106931634635 1104 74-75 0.9802769704649776 1104 76-77 0.7826016158211253 1104 78-79 0.9583662850170356 1104 80-81 0.6874047217476864 1104 82-83 0.8020231583628039 1104 84-85 1.0076932104580933 1104 86-87 0.9153402538493367 1104 88-89 1.3599156239300605 1104 90-91 1.4647206847048402 1104 92-93 1.5075439402675421 1104 94-95 1.4882487220117113 1104 96-97 1.6296796100861357 1104 98-99 2.081501307203837 1104 100-101 2.5579038839626502 1105 1 0.23666005387048727 1105 2 0.2689633407696377 1105 3 -0.25314075068096287 1105 4 -0.1084176370239831 1105 5 -0.0201929710910278 1105 6 0.23339323685810598 1105 7 0.010481072031005567 1105 8 0.2292987394035535 1105 9 0.2835985650271553 1105 10-11 0.23146784244071483 1105 12-13 0.4323512648090144 1105 14-15 0.47702617129481695 1105 16-17 0.35013075823334816 1105 18-19 0.20219539852998025 1105 20-21 0.44035277507242654 1105 22-23 0.04112550205710619 1105 24-25 -0.10789568911288683 1105 26-27 0.2577772234890219 1105 28-29 -0.1798473547424564 1105 30-31 -0.213861515362332 1105 32-33 -0.41729684183593463 1105 34-35 -0.04300216451478889 1105 36-37 -0.43339809098968374 1105 38-39 -0.843073929795743 1105 40-41 -0.9328430444223486 1105 42-43 -0.9210218482652124 1105 44-45 -1.1514547568052649 1105 46-47 -1.7561080235466875 1105 48-49 -1.8082954395509034 1105 50-51 -1.5726759837728252 1105 52-53 -1.974191928407027 1105 54-55 -2.4751674102794006 1105 56-57 -2.023867281044698 1105 58-59 -2.053200081616513 1105 60-61 -2.547055949093128 1105 62-63 -2.375076598829125 1105 64-65 -2.1522568005813376 1105 66-67 -1.7454728360406264 1105 68-69 -1.4993889472604884 1105 70-71 -1.8288307600551583 1105 72-73 -1.96930921122593 1105 74-75 -1.8654060658960248 1105 76-77 -1.9035131169037776 1105 78-79 -1.4631533441297115 1105 80-81 -1.3822389114552642 1105 82-83 -1.718620040246499 1105 84-85 -1.138149176909721 1105 86-87 -0.47148431517862477 1105 88-89 -0.6061498548472812 1105 90-91 -0.35521267760140063 1105 92-93 -0.4168729799120925 1105 94-95 -0.028410701427375784 1105 96-97 0.11396180623273722 1105 98-99 0.4772350461579187 1105 100-101 0.05167470824195064 1106 1 -0.12774672579053004 1106 2 -0.4513756422812065 1106 3 -0.3548356659352052 1106 4 -0.22706170482059207 1106 5 0.14929855433270234 1106 6 0.04695255889200922 1106 7 -0.1081629957656034 1106 8 -0.31307414195237726 1106 9 -0.18250312988809725 1106 10-11 -0.323616903321998 1106 12-13 -0.17781822671641123 1106 14-15 -0.4594145066712798 1106 16-17 0.03233414806385326 1106 18-19 -0.0011944319784973345 1106 20-21 -0.19524044526655615 1106 22-23 -0.18768805726492843 1106 24-25 -0.47230246877390414 1106 26-27 -0.5388329460025005 1106 28-29 -0.43408464287805515 1106 30-31 -0.061319142480975586 1106 32-33 -0.5444154859037269 1106 34-35 -0.24215470688767482 1106 36-37 -0.7045845316676491 1106 38-39 -1.449006133185577 1106 40-41 -1.9116566037443832 1106 42-43 -1.793903204197413 1106 44-45 -1.4692513669747527 1106 46-47 -1.014582599817878 1106 48-49 -1.1599903548051422 1106 50-51 -1.4921675091965554 1106 52-53 -2.04622582671211 1106 54-55 -2.089574189940418 1106 56-57 -1.5323418573158811 1106 58-59 -1.006589912124987 1106 60-61 -1.4623101863812629 1106 62-63 -1.1335511751003118 1106 64-65 -1.1098839192254069 1106 66-67 -0.9446253784135124 1106 68-69 -1.1307448794638795 1106 70-71 -2.1805256753093936 1106 72-73 -2.1642244654632172 1106 74-75 -2.293372167590938 1106 76-77 -2.013682608429196 1106 78-79 -2.3317974119263205 1106 80-81 -2.577154165692555 1106 82-83 -1.7779420741448035 1106 84-85 -1.1212000243673472 1106 86-87 -1.5308063490769293 1106 88-89 -1.076488837898129 1106 90-91 -1.211144880991231 1106 92-93 -1.4804323019459886 1106 94-95 -0.44790222685110237 1106 96-97 -0.58095344800455 1106 98-99 -0.1456463097742855 1106 100-101 0.0601492845131375 1107 1 0.34682954539591293 1107 2 0.133370120430655 1107 3 -0.058225496443675695 1107 4 0.010226430772632966 1107 5 -0.4608709371927162 1107 6 -0.0038948987351119513 1107 7 -0.15901045339272457 1107 8 0.05980721397982336 1107 9 0.3598697514678335 1107 10-11 0.2611288593898635 1107 12-13 0.2331987224361356 1107 14-15 0.5321109170575298 1107 16-17 0.5323341480638533 1107 18-19 0.5369411612418489 1107 20-21 0.32594599541140923 1107 22-23 0.4436678749384626 1107 24-25 0.4853246498701651 1107 26-27 0.36794671501444753 1107 28-29 0.591339085935509 1107 30-31 0.2395283151461456 1107 32-33 0.32422858189288206 1107 34-35 0.4569978354852111 1107 36-37 0.13863580731540281 1107 38-39 -0.05917562471099558 1107 40-41 0.06291966744205979 1107 42-43 0.1298256093619088 1107 44-45 0.051935073703219814 1107 46-47 -0.023057176089061215 1107 48-49 -0.299820863279713 1107 50-51 -1.2209810685185865 1107 52-53 -1.0801241317968575 1107 54-55 -1.2378792746861826 1107 56-57 -1.5196299929091026 1107 58-59 -1.349810251108039 1107 60-61 -1.2589203558727924 1107 62-63 -0.7649071073037028 1107 64-65 -0.6988669700728671 1107 66-67 0.33079835040004824 1107 68-69 -0.07566013370116664 1107 70-71 0.1584573755380667 1107 72-73 0.12391112775712187 1107 74-75 0.7701871544429579 1107 76-77 0.5541140017402917 1107 78-79 0.4944737745143577 1107 80-81 0.6050492241379537 1107 82-83 0.7390070783975666 1107 84-85 0.3999864163106217 1107 86-87 0.9691936509230707 1107 88-89 1.2158840434578018 1107 90-91 1.42021105121216 1107 92-93 2.1000761726302812 1107 94-95 1.6283689595895758 1107 96-97 1.7919279079276542 1107 98-99 1.8246926732765587 1107 100-101 1.8228611489199178 1108 1 0.29598208776879176 1108 2 0.2181158831425165 1108 3 0.4248253510139506 1108 4 0.28141287145059835 1108 5 0.03912906280728379 1108 6 0.07237628770556626 1108 7 0.02743022457337929 1108 8 0.05980721397982336 1108 9 0.40224263282376427 1108 10-11 0.19333224922037573 1108 12-13 0.2416732987073189 1108 14-15 0.6507549848541458 1108 16-17 0.12131719891131354 1108 18-19 -0.06051646587680182 1108 20-21 -0.2376133266224869 1108 22-23 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2.138875112364129 2316 96-97 2.4290883444263756 2316 98-99 2.805479764059008 2316 100-101 3.309162518782932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 1.0 5 8.0 6 26.0 7 82.0 8 116.0 9 222.0 10 357.0 11 608.0 12 899.0 13 1326.0 14 1626.0 15 2228.0 16 2812.0 17 3808.0 18 3715.0 19 2881.0 20 2450.0 21 2548.0 22 2739.0 23 2552.0 24 2691.0 25 2711.0 26 2280.0 27 1935.0 28 2260.0 29 2550.0 30 2986.0 31 3612.0 32 4324.0 33 5460.0 34 8373.0 35 13897.0 36 13276.0 37 17134.0 38 9289.0 39 426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.863537557362534 22.936961597294903 15.46332823444167 16.736172610900894 2 15.49450018520606 25.018117984313854 38.77651265037927 20.710869180100815 3 19.95926188329254 31.674288290609297 32.81592167976298 15.55052814633518 4 11.382519644467346 23.955783846451116 33.83034909184593 30.831347417235605 5 9.078378901212277 42.399890379159146 33.76080087696673 14.760929842661852 6 23.519845423073825 39.801143225183154 16.166170195636422 20.512841156106592 7 19.323725947991306 42.311408099186856 19.95974559214234 18.405120360679494 8 24.75485065614685 33.95298285162225 19.930762418484825 21.361404073746076 9 23.988406730536994 19.428387408421223 21.03373319378472 35.549472667257064 10-11 22.57346429434023 30.318009822075513 24.40503985186378 22.703486031720473 12-13 26.55382014330569 30.099830931487 25.597375412607683 17.74897351259963 14-15 23.033262351108412 30.369255169693137 28.052202126246385 18.54528035295207 16-17 16.485790194026244 34.76491425811126 28.827389099106355 19.92190644875614 18-19 15.748329442073908 30.710892842766285 34.903791965220194 18.63698574993962 20-21 17.869334192094033 28.547218420417035 35.31639964576121 18.26704774172772 22-23 17.18924228213045 30.529714149997787 35.448459646481524 16.832583921390242 24-25 17.38748893003784 31.554625231462847 35.71894372433781 15.3389421141615 26-27 16.877344970290334 31.172203346161897 36.621793529894845 15.328658153652922 28-29 14.460993478785927 31.67860880766444 37.106513163191366 16.753884550358265 30-31 16.060301102970776 31.075195233878112 37.32469205377989 15.539811609371226 32-33 14.802350857418887 32.58473552854038 36.9080589324531 15.704854681587635 34-35 15.066347810683286 32.24632429908047 37.3512408006828 15.336087089553441 36-37 16.054923998454207 31.427927347674867 36.63532139636738 15.881827257503542 38-39 15.392357364852897 30.411520314336787 37.18980981980386 17.006312501006455 40-41 15.05149077674984 30.971360016747585 37.41153167950917 16.565617526993407 42-43 15.856024862723627 30.57801001594177 36.90157968470717 16.66438543662743 44-45 15.2691730170807 30.747678224218927 36.6142128505811 17.36893590811927 46-47 16.605748329442076 30.339747202318655 35.166653248530714 17.88785121970856 48-49 16.148860800257626 29.42114161500684 35.75597777956686 18.674019805168665 50-51 15.088157153208275 30.068029949279445 36.333225988245715 18.510586909266564 52-53 15.714515739473473 30.636422188229613 35.864664680782546 17.78439739151437 54-55 15.274535061589244 29.994766926978507 34.65300700426697 20.077691007165285 56-57 17.015538201433056 30.855406166975285 32.73770227839948 19.391353353192176 58-59 16.060301102970776 31.9012156831173 33.6820706867402 18.356412527171724 60-61 16.201996618629742 33.038805249174786 32.35085741888737 18.408340713308107 62-63 14.989131309878431 33.67361726109009 32.16890749537074 19.168343933660736 64-65 14.983495692778359 33.813702600434745 31.685049512921665 19.517752193865228 66-67 15.281378310925046 32.659206183077046 31.323564930359876 20.735850575638032 68-69 15.309338732856448 31.906431524412582 31.64638490924534 21.13784483348563 70-71 15.88640206102568 31.09653007004267 31.468883342726027 21.54818452620562 72-73 15.626358586265196 30.014491586828758 32.43498913130988 21.924160695596168 74-75 15.51082843571371 30.415022945012478 31.457209564447307 22.616939054826503 76-77 15.382014330569197 29.26133161581193 32.82465179937203 22.53200225424684 78-79 15.580468561307464 29.265759600676272 32.45471379116013 22.69905804685613 80-81 15.81434667096047 28.933258191771998 32.06907656388375 23.183318573383787 82-83 15.400376787324488 28.73566920005153 32.56030207394049 23.303651938683497 84-85 15.609086480981269 28.605588274365672 31.823213760954538 23.962111483698518 86-87 15.462431817724372 28.807036612120847 31.576193064025926 24.154338506128855 88-89 15.615892440222204 28.651477336768377 30.654939215844134 25.07769100716529 90-91 15.71851827996812 27.820090655116058 31.498627291537513 24.962763773378313 92-93 15.729409870380806 28.224378069398597 31.305047902745354 24.741164157475243 94-95 15.584091458014655 27.896707189437244 31.36905241123903 25.150148941309077 96-97 15.176716850495128 27.966749859109573 31.240640850173097 25.615892440222204 98-99 15.625553498108044 27.966347315030998 30.4834554383705 25.92464374849046 100-101 15.774494807181386 27.255051928186134 30.707269946059096 26.263183318573386 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3765.0 1 2142.0 2 593.5 3 638.5 4 449.5 5 395.0 6 738.5 7 1225.5 8 1567.5 9 1715.0 10 1275.0 11 692.5 12 588.5 13 571.0 14 583.5 15 576.5 16 551.5 17 581.0 18 621.5 19 668.0 20 722.0 21 779.0 22 824.5 23 925.0 24 1067.5 25 1211.5 26 1438.0 27 1708.5 28 1883.5 29 2052.0 30 2260.0 31 2451.0 32 2679.5 33 2889.5 34 2998.0 35 2959.0 36 2962.0 37 3030.5 38 2946.5 39 2827.5 40 2807.5 41 2808.0 42 2872.0 43 2939.0 44 3046.0 45 3175.5 46 3478.0 47 4301.0 48 5928.0 49 6366.5 50 5607.0 51 4809.5 52 3504.5 53 2606.0 54 2050.5 55 1619.5 56 1376.5 57 1212.5 58 1072.0 59 874.0 60 714.5 61 590.0 62 435.5 63 352.5 64 291.5 65 208.0 66 152.0 67 108.0 68 75.0 69 59.5 70 39.0 71 20.0 72 9.0 73 6.0 74 6.0 75 5.0 76 3.5 77 3.5 78 2.5 79 1.5 80 1.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.019322115771677 3 0.0016101763143064165 4 0.0016101763143064165 5 0.1175428709443684 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.025440785766041E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012076322357298126 24-25 0.0 26-27 0.006440705257225666 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012881410514451332 36-37 0.0016101763143064165 38-39 0.0096610578858385 40-41 0.010466146042991707 42-43 0.006440705257225666 44-45 0.0044279848643426455 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0012076322357298126 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0016101763143064165 84-85 0.002817808550036229 86-87 0.0020127203928830208 88-89 0.0 90-91 0.002415264471459625 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 124210.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.43643828999276 #Duplication Level Percentage of deduplicated Percentage of total 1 79.55378226528784 39.328556476934224 2 11.497435062291345 11.3678447790033 3 3.9312759547268135 5.830448434103534 4 1.8988681703444346 3.754931164962563 5 0.9478055532937056 2.3428065373158358 6 0.5032163504600603 1.492633443362048 7 0.3273349075808159 1.132759037114564 8 0.21333767608500936 0.8437323886965622 9 0.1628531878511522 0.7245793414378875 >10 0.8093803436202264 7.095241928991225 >50 0.07165540265450697 2.6165365107479266 >100 0.06188421138343783 6.029305208920377 >500 0.009771191271069131 3.3918364060864667 >1k 0.011399723149580654 14.048788342323485 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4001 3.2211577167699867 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3342 2.690604621206022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3214 2.5875533370904114 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2169 1.7462362128653086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2007 1.6158119314064892 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1390 1.1190725384429594 TruSeq Adapter, Index 11 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1327 1.0683519845423075 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 846 0.6811045809516142 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 798 0.6424603494082602 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 729 0.5869092665646889 RNA PCR Primer, Index 11 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 722 0.5812736494646165 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.4556798969487159 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 552 0.444408662748571 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 477 0.38402705096208034 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 357 0.28741647210369536 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 355 0.28580629578938893 TruSeq Adapter, Index 11 (96% over 26bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.2729248852749376 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 319 0.2568231221318734 TruSeq Adapter, Index 11 (96% over 28bp) TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 269 0.216568714274213 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 265 0.2133483616456002 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 262 0.21093309717414055 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 239 0.19241606955961676 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 238 0.19161098140246358 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 236 0.19000080508815714 TruSeq Adapter, Index 11 (95% over 24bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 235 0.18919571693100393 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 226 0.18194992351662506 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 220 0.17711939457370582 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 194 0.1561871024877224 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 192 0.154576926173416 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 186 0.14974639723049674 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 184 0.14813622091619033 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 179 0.14411078013042428 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 178 0.1433056919732711 No Hit TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT 171 0.13767007487319863 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 168 0.13525481040173898 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.12317848804444087 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 149 0.11995813541582803 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 142 0.11432251831575559 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 141 0.11351743015860236 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 137 0.11029707752998952 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 134 0.10788181305852991 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.10466146042991707 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.10305128411561065 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.050881571532082E-4 0.0 12-13 0.0 0.0 0.0 0.0016101763143064165 0.0 14-15 0.0 0.0 0.0 0.0016101763143064165 0.0 16-17 0.0 0.0 0.0 0.0016101763143064165 0.0 18-19 0.0 0.0 0.0 0.0016101763143064165 0.0 20-21 0.0 0.0 0.0 0.0044279848643426455 0.0 22-23 0.0 0.0 0.0 0.014491586828757749 0.0 24-25 0.0 0.0 0.0 0.03703405522904758 0.0 26-27 0.0 0.0 0.0 0.07326302230094195 0.0 28-29 0.0 0.0 0.0 0.18235246759520168 0.0 30-31 0.0 0.0 0.0 0.3993237259479913 0.0 32-33 0.0 0.0 0.0 0.7020368730375977 0.0 34-35 0.0 0.0 0.0 1.1307463167216811 0.0 36-37 0.0 0.0 0.0 1.6158119314064892 0.0 38-39 0.0 0.0 0.0 2.157233717092022 0.0 40-41 0.0 0.0 0.0 2.6648418001771192 0.0 42-43 0.0 0.0 0.0 3.1120682714757266 0.0 44-45 0.0 0.0 0.0 3.5709685210530555 0.0 46-47 0.0 0.0 0.0 4.071733354802351 0.0 48-49 0.0 0.0 0.0 4.520167458336688 0.0 50-51 0.0 0.0 0.0 4.958135415828033 0.0 52-53 0.0 0.0 0.0 5.339344658240078 0.0 54-55 0.0 0.0 0.0 5.75154979470252 0.0 56-57 0.0 0.0 0.0 6.238628129780212 0.0 58-59 0.0 0.0 0.0 6.6488205458497704 0.0 60-61 0.0 0.0 0.0 7.086788503341117 0.0 62-63 0.0 0.0 0.0 7.490942758232027 0.0 64-65 0.0 0.0 0.0 7.902745350615893 0.0 66-67 0.0 0.0 0.0 8.377747363336287 0.0 68-69 0.0 0.0 0.0 8.866838418806859 0.0 70-71 0.0 0.0 0.0 9.378069398599147 0.0 72-73 0.0 0.0 0.0 9.84824088237662 0.0 74-75 0.0 0.0 0.0 10.34739553981161 0.0 76-77 0.0 0.0 0.0 10.820787376217696 0.0 78-79 0.0 0.0 0.0 11.384349086224942 0.0 80-81 0.0 0.0 0.0 11.930601400853394 0.0 82-83 0.0 0.0 0.0 12.441027292488528 0.0 84-85 0.0 0.0 0.0 12.996538120924242 0.0 86-87 0.0 0.0 0.0 13.54198534739554 0.0 88-89 0.0 0.0 0.0 14.092263102809758 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGTG 25 0.0050130347 56.993477 9 GAAGAGT 45 1.5091031E-5 52.771736 6 TTTCGAG 40 5.3512445E-4 47.532837 1 CGAGACA 40 5.3512445E-4 47.532837 4 TCGAGAC 40 5.3512445E-4 47.532837 3 TTCGAGA 45 9.5593015E-4 42.251408 2 ATGGGGA 235 0.0 30.340109 5 TGGGGAA 205 1.8189894E-12 30.118505 6 TTAATCC 95 3.449763E-5 30.020739 4 CATGGGG 400 0.0 26.14306 4 CTTTAAT 165 8.229108E-8 25.927002 2 GGTATCA 2660 0.0 25.374672 1 TAATCCC 115 1.266814E-4 24.799742 5 CCTTTAA 165 2.3866087E-6 23.046225 1 TTTAATC 150 2.9891902E-5 22.18199 3 GTACTTT 1900 0.0 21.247568 14-15 GAGTACT 1990 0.0 20.525288 12-13 CATGGGC 210 8.425268E-7 20.371218 4 GTACATG 1175 0.0 20.22674 1 GTATCAA 3320 0.0 20.18714 2 >>END_MODULE