##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937961_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 125659 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35343270279088 33.0 31.0 34.0 31.0 34.0 2 32.548699257514386 34.0 31.0 34.0 31.0 34.0 3 32.533531223390284 34.0 31.0 34.0 30.0 34.0 4 36.06033789859859 37.0 35.0 37.0 35.0 37.0 5 36.054616064110014 37.0 35.0 37.0 35.0 37.0 6 36.15226923658472 37.0 36.0 37.0 35.0 37.0 7 36.10890584836741 37.0 36.0 37.0 35.0 37.0 8 36.149539627086 37.0 36.0 37.0 35.0 37.0 9 37.742644776737045 39.0 38.0 39.0 35.0 39.0 10-11 37.78691538210553 39.0 38.0 39.0 35.0 39.0 12-13 37.72798207848224 39.0 38.0 39.0 35.0 39.0 14-15 38.831671428230365 40.0 38.0 41.0 34.5 41.0 16-17 38.741017356496556 40.0 38.0 41.0 34.5 41.0 18-19 38.824306257410925 40.0 38.0 41.0 35.0 41.0 20-21 38.85594744506959 40.0 38.0 41.0 35.0 41.0 22-23 38.67503322483865 40.0 38.0 41.0 35.0 41.0 24-25 38.5283783891325 40.0 38.0 41.0 35.0 41.0 26-27 38.26934401833533 40.0 37.0 41.0 34.0 41.0 28-29 38.062371179143554 40.0 36.5 41.0 34.0 41.0 30-31 37.55818126835324 39.5 35.5 41.0 33.5 41.0 32-33 37.008109248044306 39.0 35.0 41.0 33.0 41.0 34-35 36.59140610700388 38.0 35.0 41.0 33.0 41.0 36-37 36.259647140276456 38.0 35.0 41.0 32.5 41.0 38-39 35.842558034044515 38.0 35.0 41.0 31.0 41.0 40-41 35.627225268385075 38.0 35.0 41.0 30.5 41.0 42-43 35.20569159391687 38.0 35.0 41.0 28.5 41.0 44-45 34.630822304809044 38.0 35.0 41.0 24.0 41.0 46-47 33.80342832586604 38.0 35.0 41.0 11.5 41.0 48-49 33.01558185247376 38.0 34.0 41.0 2.0 41.0 50-51 32.374378277719856 37.5 33.0 40.0 2.0 41.0 52-53 31.812763112868954 37.0 33.0 40.0 2.0 41.0 54-55 31.364967889287676 36.5 33.0 40.0 2.0 41.0 56-57 30.861498181586676 36.0 31.5 40.0 2.0 41.0 58-59 30.25256845908371 35.5 30.5 40.0 2.0 41.0 60-61 29.501273287229722 35.0 28.5 40.0 2.0 41.0 62-63 28.886140268504445 35.0 26.5 39.0 2.0 41.0 64-65 28.213713303464136 35.0 23.5 39.0 2.0 41.0 66-67 27.44948630818326 35.0 13.5 38.5 2.0 40.0 68-69 26.71243603721182 35.0 2.0 37.5 2.0 40.0 70-71 26.090220358271196 35.0 2.0 37.0 2.0 39.5 72-73 25.676338344249118 34.0 2.0 36.0 2.0 39.0 74-75 25.29659634407404 34.0 2.0 36.0 2.0 39.0 76-77 24.15520575525828 32.0 2.0 35.0 2.0 36.5 78-79 24.652185677110275 33.5 2.0 35.0 2.0 37.0 80-81 24.47698135430013 34.0 2.0 35.0 2.0 37.0 82-83 24.152850173883287 33.5 2.0 35.0 2.0 36.0 84-85 23.837321640312275 33.0 2.0 35.0 2.0 36.0 86-87 23.507333338638695 33.0 2.0 35.0 2.0 35.5 88-89 23.15929619048377 33.0 2.0 35.0 2.0 35.0 90-91 22.89045352899514 33.0 2.0 35.0 2.0 35.0 92-93 22.599913257307477 32.0 2.0 35.0 2.0 35.0 94-95 22.34202484501707 32.0 2.0 35.0 2.0 35.0 96-97 21.931310132978936 32.0 2.0 35.0 2.0 35.0 98-99 21.488142512673186 31.0 2.0 35.0 2.0 35.0 100-101 19.875384970435864 28.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09916408203081772 1101 2 -0.0961216622626182 1101 3 0.0814575932882704 1101 4 0.00535290468775429 1101 5 -0.03404138082294139 1101 6 0.15818755428551867 1101 7 0.1204351609424279 1101 8 0.0441451145713998 1101 9 -0.30511227886341885 1101 10-11 -0.23183874274508298 1101 12-13 -0.1350801155274226 1101 14-15 -0.22930070559196736 1101 16-17 -0.4608868978870859 1101 18-19 -0.34356958699311946 1101 20-21 -0.5418745970486469 1101 22-23 -0.3702050476777927 1101 24-25 -0.5775916095052764 1101 26-27 -0.323695101977556 1101 28-29 -0.2713709667434969 1101 30-31 -0.20117513816097699 1101 32-33 -0.6844373583126497 1101 34-35 -0.6115721170131039 1101 36-37 -0.347311149356365 1101 38-39 -0.0950266210238837 1101 40-41 -0.8682171888825465 1101 42-43 -0.624340794628722 1101 44-45 -0.19850366888251614 1101 46-47 0.4719649454886792 1101 48-49 0.4278683664866918 1101 50-51 0.3562593378526273 1101 52-53 0.26640162826363323 1101 54-55 0.8896875912402784 1101 56-57 0.9106629937612531 1101 58-59 0.8803384916965236 1101 60-61 1.1131405693503673 1101 62-63 1.4399399389510705 1101 64-65 1.4137138329873515 1101 66-67 0.9089835490229028 1101 68-69 1.6995400170812367 1101 70-71 2.004325487170348 1101 72-73 1.8035685047366137 1101 74-75 1.8654828544009874 1101 76-77 1.7264676296746018 1101 78-79 1.5253257102998 1101 80-81 1.1038388341269574 1101 82-83 1.3585075515569507 1101 84-85 1.4724808394510163 1101 86-87 1.2648901930535708 1101 88-89 1.7040388087601066 1101 90-91 1.7519908377430369 1101 92-93 2.3179044079940816 1101 94-95 2.0081662191384844 1101 96-97 1.4622595706550001 1101 98-99 1.6715805406101083 1101 100-101 1.3798377968065019 1102 1 -0.013537170715828495 1102 2 0.03365172439150399 1102 3 0.003985625908050849 1102 4 -0.1718603000483867 1102 5 -0.12076607305222353 1102 6 0.06848317100315171 1102 7 0.12976629871393186 1102 8 -0.06429995717642356 1102 9 -0.20764501625854592 1102 10-11 -0.2283101612348446 1102 12-13 0.22950076740208658 1102 14-15 -0.016488034063698365 1102 16-17 0.0516591697048554 1102 18-19 0.10577566510441727 1102 20-21 0.12451762438944769 1102 22-23 -0.4401101680416275 1102 24-25 0.013092935307710718 1102 26-27 -0.040075561477287636 1102 28-29 0.19083400463578215 1102 30-31 0.3326208314148076 1102 32-33 0.2425210044255266 1102 34-35 0.820051814610828 1102 36-37 0.8365671537879962 1102 38-39 0.764731867174973 1102 40-41 0.31695492748222875 1102 42-43 0.6157987803732325 1102 44-45 0.3824968800079418 1102 46-47 -0.12001341254472919 1102 48-49 -0.3672778736136735 1102 50-51 -0.7138026083561044 1102 52-53 -0.9521743930646842 1102 54-55 -0.3208903119981805 1102 56-57 -1.1406582639820222 1102 58-59 -0.994592896996334 1102 60-61 -0.6611086269171729 1102 62-63 -1.3061198116958366 1102 64-65 -0.3627309251087816 1102 66-67 -1.2824616011378431 1102 68-69 -1.007352478802087 1102 70-71 -1.5342536706748646 1102 72-73 -1.4039512945263084 1102 74-75 -1.0682582261290854 1102 76-77 -1.1005926698627633 1102 78-79 -1.5098424854188544 1102 80-81 -1.0898410843236057 1102 82-83 -0.9627501917191417 1102 84-85 -1.1830119857665764 1102 86-87 -1.2257002562524733 1102 88-89 -1.5310039262826294 1102 90-91 -1.497597494414105 1102 92-93 -1.1776260554262912 1102 94-95 -1.7567910458187797 1102 96-97 -1.0084532028100668 1102 98-99 -0.8632740034187485 1102 100-101 -1.0319476654376771 1103 1 0.22223518700774747 1103 2 0.1585214346270689 1103 3 0.15574714352322871 1103 4 0.0030406797306383737 1103 5 0.11834170264091881 1103 6 -0.03991791300768455 1103 7 0.18271605898076615 1103 8 0.1908595174952481 1103 9 0.18030578632640726 1103 10-11 0.17444155859004695 1103 12-13 0.03583806154426128 1103 14-15 0.15340981876098425 1103 16-17 0.34861560080762644 1103 18-19 0.25503561924242746 1103 20-21 0.2188474138411891 1103 22-23 0.0754203718791544 1103 24-25 0.0947064437504821 1103 26-27 0.04883417377391197 1103 28-29 0.16571413805250046 1103 30-31 -0.1916651806760825 1103 32-33 -0.19931764473019342 1103 34-35 -0.22539325522448195 1103 36-37 -0.3969121040815011 1103 38-39 -0.1831522270505772 1103 40-41 -0.1870058813566331 1103 42-43 -0.2926856890868521 1103 44-45 -0.9843990966441325 1103 46-47 -0.8539095976604258 1103 48-49 -0.9252307608348538 1103 50-51 -0.5886202026858953 1103 52-53 -0.45821983813451794 1103 54-55 -0.675278471264388 1103 56-57 -0.10261365276668144 1103 58-59 0.053979127185446174 1103 60-61 -0.48057912931450986 1103 62-63 -0.6810416378371755 1103 64-65 -0.8828476647377137 1103 66-67 -0.7216944550048403 1103 68-69 -0.995470052285512 1103 70-71 -0.5842849402183283 1103 72-73 -0.6241931123290989 1103 74-75 -0.5872284158309817 1103 76-77 -0.19804941366096784 1103 78-79 -0.22122459923999216 1103 80-81 0.1432212462997029 1103 82-83 0.48836508866443396 1103 84-85 0.4595771324322975 1103 86-87 0.5976268231721669 1103 88-89 0.6024127924999441 1103 90-91 -0.19397022737220482 1103 92-93 0.31518194957683576 1103 94-95 0.3618247557238483 1103 96-97 0.5791015188909938 1103 98-99 0.2629507203669519 1103 100-101 -0.28022784054712346 1104 1 0.1296919959508358 1104 2 0.18462661755389576 1104 3 0.4249925703524937 1104 4 0.28860978542169846 1104 5 0.2444849953238446 1104 6 -0.05998905121906972 1104 7 0.2725280508506813 1104 8 0.04407344025947424 1104 9 -0.12184106326709099 1104 10-11 0.0736730118420752 1104 12-13 0.09925904731661461 1104 14-15 0.4065221155089489 1104 16-17 0.36322434064502573 1104 18-19 0.31013597493348044 1104 20-21 0.4033303914834647 1104 22-23 0.49805756700110493 1104 24-25 0.3541386083846305 1104 26-27 0.30470942783895083 1104 28-29 0.5547829896785217 1104 30-31 0.42199716688489275 1104 32-33 0.62232869673322 1104 34-35 1.1032716329868961 1104 36-37 1.127065284129877 1104 38-39 1.1469100188843768 1104 40-41 1.089925012952314 1104 42-43 1.266606739774943 1104 44-45 1.5017543586404187 1104 46-47 1.7830314592501466 1104 48-49 1.6308858550188035 1104 50-51 2.0308221245498785 1104 52-53 2.0853523163370262 1104 54-55 2.2513920063778876 1104 56-57 2.3514958492658415 1104 58-59 1.7954044320634956 1104 60-61 2.0749023239375255 1104 62-63 1.9975660857400612 1104 64-65 2.3707092458313888 1104 66-67 2.4723794779219865 1104 68-69 2.6502997647876576 1104 70-71 2.60807405368411 1104 72-73 2.7568726905164276 1104 74-75 2.604050801648693 1104 76-77 2.6228982489406576 1104 78-79 2.9133345979144813 1104 80-81 3.0681450267875086 1104 82-83 2.390581806144109 1104 84-85 2.116367020643679 1104 86-87 2.0704736219526545 1104 88-89 1.8915388087601066 1104 90-91 2.3556677299448694 1104 92-93 2.7074901304711467 1104 94-95 2.2737912191384844 1104 96-97 2.214804008728393 1104 98-99 2.0758953341880897 1104 100-101 1.9629441614853995 1105 1 0.1140669959508358 1105 2 -0.06537338244610424 1105 3 0.06561757035249371 1105 4 0.06204728542169846 1105 5 0.0413599953238446 1105 6 0.09626094878093028 1105 7 -0.032159449149318675 1105 8 -0.47936405974052576 1105 9 -0.129653563267091 1105 10-11 0.0971105118420752 1105 12-13 0.1305090473166146 1105 14-15 0.3440221155089489 1105 16-17 0.37103684064502573 1105 18-19 0.17732347493348044 1105 20-21 0.17676789148346472 1105 22-23 0.28712006700110493 1105 24-25 0.11976360838463052 1105 26-27 0.35158442783895083 1105 28-29 -0.1444357603214783 1105 30-31 0.23840341688489275 1105 32-33 0.15357869673322 1105 34-35 0.04077163298689612 1105 36-37 0.31847153412987694 1105 38-39 0.5062850188843768 1105 40-41 0.5118000129523139 1105 42-43 0.747075489774943 1105 44-45 0.8220668586404187 1105 46-47 0.8103752092501466 1105 48-49 1.3769796050188035 1105 50-51 0.6558221245498785 1105 52-53 0.038477316337026224 1105 54-55 0.4701420063778876 1105 56-57 0.23430834926584154 1105 58-59 0.5414981820634956 1105 60-61 0.6803710739375255 1105 62-63 0.5327223357400612 1105 64-65 0.25742799583138876 1105 66-67 -0.07449552207801347 1105 68-69 -0.3731377352123424 1105 70-71 -0.3567696963158902 1105 72-73 -0.09859605948357242 1105 74-75 0.3071758016486932 1105 76-77 0.017429498940657595 1105 78-79 -0.01635290208551865 1105 80-81 -0.13888622321249144 1105 82-83 -0.21879319385589113 1105 84-85 -0.1648829793563209 1105 86-87 0.0665673719526545 1105 88-89 0.3173200587601066 1105 90-91 0.3087927299448694 1105 92-93 -0.0034473695288532724 1105 94-95 -0.29652128086151563 1105 96-97 -0.8750397412716069 1105 98-99 -0.5217609158119103 1105 100-101 0.002006661485399519 1106 1 -0.0421830040491642 1106 2 0.059626617553895755 1106 3 -0.06719492964750629 1106 4 0.21048478542169846 1106 5 0.1194849953238446 1106 6 -0.013114051219069722 1106 7 0.2803405508506813 1106 8 0.04407344025947424 1106 9 0.143783936732909 1106 10-11 0.1830480118420752 1106 12-13 -0.3382409526833854 1106 14-15 -0.3395716344910511 1106 16-17 0.12884934064502573 1106 18-19 -0.11564527506651956 1106 20-21 0.4267678914834647 1106 22-23 -0.05272368299889507 1106 24-25 -0.10289264161536948 1106 26-27 0.03517817783895083 1106 28-29 0.2735329896785217 1106 30-31 -0.12097158311510725 1106 32-33 -0.40892130326678 1106 34-35 -0.5803221170131039 1106 36-37 -0.5604347158701231 1106 38-39 -0.2944962311156232 1106 40-41 -0.5741374870476861 1106 42-43 -0.432612010225057 1106 44-45 -0.46699564135958127 1106 46-47 -0.4396247907498534 1106 48-49 -0.24020789498119655 1106 50-51 0.10504087454987854 1106 52-53 0.16347731633702622 1106 54-55 -0.5845454936221124 1106 56-57 -0.19537915073415846 1106 58-59 -0.46631431793650435 1106 60-61 -1.1868164260624745 1106 62-63 -0.6899339142599388 1106 64-65 -0.48475950416861124 1106 66-67 -0.43777677207801347 1106 68-69 -1.0372002352123424 1106 70-71 -1.0872384463158902 1106 72-73 -0.8681273094835724 1106 74-75 -0.5365741983513068 1106 76-77 -0.7521017510593424 1106 78-79 -0.43041540208551865 1106 80-81 -0.7951362232124914 1106 82-83 -0.8242619438558911 1106 84-85 -0.2117579793563209 1106 86-87 -0.2967138780473455 1106 88-89 -0.7022111912398934 1106 90-91 -1.3357385200551306 1106 92-93 -1.4292286195288533 1106 94-95 -1.0504275308615156 1106 96-97 -0.9375397412716069 1106 98-99 -0.8264484158119103 1106 100-101 0.09966291148539952 1107 1 0.18298024221949305 1107 2 0.15069471456882155 1107 3 0.09768380169577995 1107 4 0.24196799437692107 1107 5 0.14910253263727924 1107 6 0.13730572490033666 1107 7 0.30727618517904176 1107 8 0.22865832831917743 1107 9 0.21549569046425177 1107 10-11 0.1350652693047607 1107 12-13 0.14747499880915171 1107 14-15 -0.031094488968662404 1107 16-17 -0.0012812563699000634 1107 18-19 0.14694800851557233 1107 20-21 0.10056686536405834 1107 22-23 0.4553803281951332 1107 24-25 0.43990120166821356 1107 26-27 0.4716287375404491 1107 28-29 0.7803543516188185 1107 30-31 0.7203297228550412 1107 32-33 0.36020183106158044 1107 34-35 0.7988756441809315 1107 36-37 0.8027299296522656 1107 38-39 0.4910681345560164 1107 40-41 0.5330220278776849 1107 42-43 1.075200489774943 1107 44-45 0.24172637356578974 1107 46-47 0.07640832492179328 1107 48-49 0.6934441572576162 1107 50-51 0.6534317327588326 1107 52-53 0.11170492827732303 1107 54-55 0.027336502646544858 1107 56-57 -0.2997984604356496 1107 58-59 -0.15060769480217573 1107 60-61 -0.049806164868446245 1107 62-63 -0.25196749634949 1107 64-65 -0.2626862765566713 1107 66-67 -0.3825062496899534 1107 68-69 -0.010031391928759348 1107 70-71 0.30577694547515577 1107 72-73 0.502733231561205 1107 74-75 0.4781762680666013 1107 76-77 0.13164358476155513 1107 78-79 0.31602816134731526 1107 80-81 0.640673011862134 1107 82-83 0.5612324591291831 1107 84-85 0.006234091539198516 1107 86-87 0.29429591672877464 1107 88-89 0.26000895801383805 1107 90-91 0.42825401725830403 1107 92-93 0.06051018644129513 1107 94-95 7.035325713182772E-4 1107 96-97 0.020249437832870143 1107 98-99 0.5601537297104784 1107 100-101 0.7278112323809189 1108 1 0.3122204050417423 1108 2 0.18438987512965355 1108 3 -0.11217599025356861 1108 4 0.20148857330048742 1108 5 0.14457969229354006 1108 6 0.27050337302335237 1108 7 -0.10294543399780309 1108 8 0.045257152380685284 1108 9 0.4271646185510889 1108 10-11 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1.7410300011608015 2316 94-95 1.3116144950005513 2316 96-97 1.6289473276939077 2316 98-99 1.5157121445329196 2316 100-101 1.1380788597612614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 13.0 6 27.0 7 68.0 8 98.0 9 183.0 10 355.0 11 601.0 12 877.0 13 1295.0 14 1560.0 15 2200.0 16 2767.0 17 3694.0 18 3779.0 19 2704.0 20 2280.0 21 2413.0 22 2462.0 23 2587.0 24 2462.0 25 2684.0 26 2228.0 27 1556.0 28 1607.0 29 1892.0 30 2210.0 31 2511.0 32 3072.0 33 3860.0 34 5775.0 35 12142.0 36 12013.0 37 19958.0 38 19403.0 39 2319.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.02582385662785 22.85630157808036 15.432241224265672 16.68563334102611 2 15.497273848847854 24.961993075178096 38.78457436223982 20.75615871373423 3 19.970235252920766 31.673988476108615 32.9895266291026 15.366249641868016 4 11.103064643201044 23.82399987267128 34.02700960536054 31.04592587876714 5 9.021240022600848 42.230958387381726 33.60682481955132 15.140976770466102 6 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36.72964184937695 15.120809669549438 28-29 14.645588457651263 31.562005109065012 37.144175904630785 16.64823052865294 30-31 16.101114922130527 31.066218893990882 37.56953341981076 15.263132764067835 32-33 14.74506402247352 32.557954464065446 37.02281571554764 15.6741657979134 34-35 14.954888983519654 32.24552173168994 37.53427601494813 15.26531326984228 36-37 16.01938656946854 31.406879207983827 36.7302274499817 15.843506772565934 38-39 15.370043141187894 30.220720505595615 37.43612397917761 16.973112374038877 40-41 14.976579817652889 30.730383358736702 37.76957087540145 16.523465948208962 42-43 15.786750924219934 30.33510682748823 37.31779237782191 16.560349870469928 44-45 15.074451067622737 30.799105473009003 36.81726659928533 17.309176860082925 46-47 16.536022091533436 30.229032540446767 35.42205492642787 17.81289044159193 48-49 16.109072967316308 29.45670425516676 35.827517328643395 18.60670544887354 50-51 14.976643137379734 30.158603840552605 36.46973157513588 18.395021446931775 52-53 15.454125848526568 30.90188526090451 35.930176111539964 17.71381277902896 54-55 15.284221583810154 30.17173461510915 34.81923300360499 19.72481079747571 56-57 16.761234770291026 31.100836390549023 32.87548046697809 19.262448372181858 58-59 15.925640025784066 32.0518227902498 33.78627873849068 18.236258445475453 60-61 16.321950676035936 33.10029524347639 32.357809627643064 18.2199444528446 62-63 14.88114659515037 33.76996474585982 32.33194598078928 19.016942678200525 64-65 14.76575493995655 33.76757733230409 31.98059828583707 19.48606944190229 66-67 15.1338145297989 32.750539157561334 31.55484286839781 20.560803444241955 68-69 15.214709767782395 32.04093651021026 31.906842381702717 20.837511340304637 70-71 15.760908490438407 31.129485353217834 31.994126962652892 21.115479193690863 72-73 15.458900675638038 30.03127511758012 32.779188120230145 21.730636086551698 74-75 15.377728614743075 30.55929141565666 31.871573066791875 22.191406902808396 76-77 15.389267780262456 29.34608742708441 33.03503927295299 22.22960551970014 78-79 15.546041270422334 29.30788881019266 32.8281300981227 22.317939821262307 80-81 15.847253280704127 28.84791379845455 32.34308724404937 22.961745676791953 82-83 15.415131427116243 28.739684383927933 32.5376614488417 23.307522740114116 84-85 15.662602661233846 28.659196536576047 32.0732794295537 23.604921372636404 86-87 15.554857013254178 28.824154354858607 31.682297655948467 23.938690975938755 88-89 15.50227202190054 28.503728344169538 31.31132668571292 24.682672948217 90-91 15.813829490923851 27.917618316236798 31.55802608647212 24.71052610636723 92-93 15.717935046435194 27.92597426368187 31.66705130551731 24.689039384365625 94-95 15.417916742931267 27.943879865349874 31.464121153279905 25.17408223843895 96-97 15.058213100533985 27.805011976858008 31.581502319770173 25.555272602837835 98-99 15.441392976229318 28.025847730763413 30.720043928409424 25.81271536459784 100-101 15.402398554818994 27.68404969003414 31.138637105181484 25.774914649965382 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3778.0 1 2140.0 2 573.5 3 636.5 4 464.0 5 411.5 6 790.5 7 1269.5 8 1632.5 9 1740.5 10 1294.0 11 769.5 12 633.5 13 611.5 14 599.5 15 593.0 16 575.0 17 589.5 18 641.5 19 675.5 20 709.5 21 790.5 22 895.5 23 1021.0 24 1169.0 25 1276.5 26 1487.0 27 1810.5 28 1916.5 29 2070.5 30 2335.0 31 2521.5 32 2734.0 33 2882.0 34 2980.5 35 3003.0 36 2995.0 37 3029.0 38 3003.5 39 2832.0 40 2773.5 41 2847.5 42 2924.5 43 3027.0 44 3034.0 45 3112.5 46 3446.0 47 4287.0 48 6092.5 49 6393.5 50 5551.0 51 4868.5 52 3533.0 53 2611.0 54 2037.0 55 1630.0 56 1352.0 57 1171.0 58 1057.0 59 867.5 60 715.0 61 623.5 62 465.0 63 343.5 64 265.5 65 196.5 66 154.0 67 101.5 68 57.0 69 36.5 70 30.5 71 21.5 72 11.5 73 10.5 74 6.5 75 4.0 76 3.0 77 2.5 78 2.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.019099308445873354 3 0.005570631630046396 4 0.0 5 0.0 6 0.0 7 0.0 8 7.958045185780565E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005172729370757367 26-27 0.021884624260896553 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.019497210705162384 36-37 0.0039790225928902825 38-39 0.01989511296445141 40-41 0.015518188112272102 42-43 0.009947556482225705 44-45 0.004774827111468339 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.958045185780565E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0023874135557341693 86-87 3.9790225928902824E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 125659.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.191860511383986 #Duplication Level Percentage of deduplicated Percentage of total 1 79.24580192189472 38.9824843425461 2 11.874332675445693 11.682410332725869 3 3.9926230303814667 5.89213665555193 4 1.7617368233733457 3.4665244829260136 5 0.9140324198401656 2.2481477649830097 6 0.5273886174652991 1.5565936383386785 7 0.3170802730772964 1.0918437994890935 8 0.22972142233151066 0.9040339331046721 9 0.1650111625198175 0.7305485480546559 >10 0.821820299608503 7.330155420622478 >50 0.06471025981169314 2.264859659873149 >100 0.06471025981169314 6.340174599511377 >500 0.009706538971753971 3.4251426479599547 >1k 0.0113242954670463 14.084944174313021 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3955 3.147406870976213 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3512 2.7948654692461345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3260 2.594322730564464 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2248 1.788968557763471 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2069 1.646519548937999 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1336 1.0631948368202835 TruSeq Adapter, Index 11 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1319 1.0496661600044566 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 948 0.7544226836119976 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 821 0.6533555097525844 RNA PCR Primer, Index 11 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 717 0.5705918398204666 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 711 0.5658170127089981 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 596 0.47429949307252167 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 511 0.40665610899338683 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 500 0.3979022592890282 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 473 0.3764155372874207 TruSeq Adapter, Index 11 (96% over 28bp) CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 397 0.31593439387548844 TruSeq Adapter, Index 11 (96% over 26bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 368 0.2928560628367248 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 366 0.29126445379956867 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 264 0.2100923929046069 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 258 0.20531756579313856 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 257 0.20452176127456054 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 245 0.19497210705162385 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 238 0.18940147542157745 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 237 0.1886056709029994 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 237 0.1886056709029994 TruSeq Adapter, Index 11 (95% over 24bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 229 0.18223923475437492 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 214 0.1703021669757041 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 211 0.16791475341996992 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 203 0.16154831727134547 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 188 0.14961124949267463 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 184 0.1464280314183624 No Hit TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT 173 0.13767418171400378 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 166 0.13210355008395738 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 164 0.13051194104680128 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 162 0.12892033200964514 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.12175809134244264 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 152 0.12096228682386459 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 150 0.11937067778670847 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 143 0.11380004615666207 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 135 0.10743361000803762 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 132 0.10504619645230345 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.10186297837799123 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.958045185780565E-4 0.0 3 0.0 0.0 0.0 7.958045185780565E-4 0.0 4 0.0 0.0 0.0 7.958045185780565E-4 0.0 5 0.0 0.0 0.0 7.958045185780565E-4 0.0 6 0.0 0.0 0.0 7.958045185780565E-4 0.0 7 0.0 0.0 0.0 7.958045185780565E-4 0.0 8 0.0 0.0 0.0 7.958045185780565E-4 0.0 9 0.0 0.0 0.0 7.958045185780565E-4 0.0 10-11 0.0 0.0 0.0 7.958045185780565E-4 0.0 12-13 0.0 0.0 0.0 7.958045185780565E-4 0.0 14-15 0.0 0.0 0.0 7.958045185780565E-4 0.0 16-17 0.0 0.0 0.0 7.958045185780565E-4 0.0 18-19 0.0 0.0 0.0 0.001591609037156113 0.0 20-21 0.0 0.0 0.0 0.0043769248521793105 0.0 22-23 0.0 0.0 0.0 0.017109797149428216 0.0 24-25 0.0 0.0 0.0 0.04337134626250408 0.0 26-27 0.0 0.0 0.0 0.10345458741514735 0.0 28-29 0.0 0.0 0.0 0.1989511296445141 0.0 30-31 0.0 0.0 0.0 0.4106351315862772 0.0 32-33 0.0 0.0 0.0 0.6959310514965104 0.0 34-35 0.0 0.0 0.0 1.0950270175634058 0.0 36-37 0.0 0.0 0.0 1.607525127527674 0.0 38-39 0.0 0.0 0.0 2.1653840950508916 0.0 40-41 0.0 0.0 0.0 2.7001647315353456 0.0 42-43 0.0 0.0 0.0 3.1346739986789647 0.0 44-45 0.0 0.0 0.0 3.575549701971208 0.0 46-47 0.0 0.0 0.0 4.068948503489603 0.0 48-49 0.0 0.0 0.0 4.552797650785061 0.0 50-51 0.0 0.0 0.0 4.96701390270494 0.0 52-53 0.0 0.0 0.0 5.3271154473615105 0.0 54-55 0.0 0.0 0.0 5.737750578947788 0.0 56-57 0.0 0.0 0.0 6.200510906500927 0.0 58-59 0.0 0.0 0.0 6.6234810081251645 0.0 60-61 0.0 0.0 0.0 7.050032230083003 0.0 62-63 0.0 0.0 0.0 7.453903023261367 0.0 64-65 0.0 0.0 0.0 7.8533968915875505 0.0 66-67 0.0 0.0 0.0 8.281141820323256 0.0 68-69 0.0 0.0 0.0 8.777723839915964 0.0 70-71 0.0 0.0 0.0 9.285845025028053 0.0 72-73 0.0 0.0 0.0 9.75815500680413 0.0 74-75 0.0 0.0 0.0 10.24439156765532 0.0 76-77 0.0 0.0 0.0 10.727047008172912 0.0 78-79 0.0 0.0 0.0 11.266602471768834 0.0 80-81 0.0 0.0 0.0 11.82963416866281 0.0 82-83 0.0 0.0 0.0 12.384707820371005 0.0 84-85 0.0 0.0 0.0 12.938587765301332 0.0 86-87 0.0 0.0 0.0 13.46302294304427 0.0 88-89 0.0 0.0 0.0 14.02366722638251 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTCA 25 0.0050114826 56.99833 3 ACCTACC 35 2.778775E-4 54.284122 7 TAGCACA 40 5.370541E-4 47.49861 4 TAGACAG 60 8.250061E-5 39.582172 5 GTAAACC 60 0.003938254 31.665737 3 ATGGGCA 80 4.4719133E-4 29.686632 5 CCTACCT 65 0.0058253757 29.229914 8 TGGGGTG 65 0.0058253757 29.229914 6 CTTCGGT 35 0.008380515 27.142061 48-49 CCTATAG 70 0.008364847 27.142061 1 GTACATA 95 0.0012206914 24.999268 1 CCACGAC 50 0.0016503466 23.749304 90-91 GGTATCA 2770 0.0 23.320618 1 GAGTACT 2050 0.0 22.1274 12-13 AGTACTT 1875 0.0 21.912691 12-13 GTACTTT 2015 0.0 21.68671 14-15 GTATCAA 3560 0.0 21.347689 1 ATGGGGG 140 4.8196904E-4 20.356546 5 ACTTTTT 2180 0.0 20.154226 16-17 CATGGGG 455 0.0 19.834583 4 >>END_MODULE