FastQCFastQC Report
Thu 26 May 2016
SRR937959_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937959_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343452
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99180.42322181124256014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76960.32840442219426724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50690.21630483577218562No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34430.14692001372334487No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31820.13578259763801434No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA28750.12268226530775966No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27610.11781764678773024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.048.2075961
GTATCAA79550.042.623571
ATCAACG99900.033.3506933
TATCAAC100850.033.130792
TCAACGC101600.032.745884
CAACGCA103300.032.1609765
AACGCAG106250.031.2613586
GTACATG78000.027.2073211
TACATGG76250.026.802852
ACGCAGA123750.026.7947257
CGCAGAG123800.026.6304138
GCAGAGT133000.024.1795549
ACATGGG81250.023.9835153
TATACCG1201.7046547E-423.7641545
TGGTATC20900.023.650452
ATACCGA1754.038804E-621.722596
GGACCGA6400.021.5316666
GAGTACT86500.021.50057612-13
AGTACTT88550.021.2978812-13
AGAGTAC119100.021.1992910-11
Produced by FastQC (version 0.11.5)