FastQCFastQC Report
Thu 26 May 2016
SRR937959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2343452
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98490.4202774368751739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79350.33860305224941667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52090.22227892869152002No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33520.1430368533257775No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32590.13906834874364826No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30810.1314727163176374No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26960.11504396078946784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47800.048.0587161
GTATCAA81400.040.2575871
ATCAACG100650.032.266943
TCAACGC101900.031.8704454
TATCAAC103450.031.630852
CAACGCA103800.031.3322125
AACGCAG106400.030.4427386
ACGCAGA124150.025.8989947
CGCAGAG124350.025.780958
TGGTATC23400.024.593622
ATACCGT2402.3101165E-1023.747126
GTACATG77900.023.6890281
TAAGGTG14050.023.3509795
GCAGAGT135750.023.336039
GTAAGGT12500.023.2039224
TACATGG78350.022.8852312
GAGTACT85600.022.41550412-13
ACCGTCG1553.912308E-521.449018
GTACTTT88950.021.43781514-15
GTGGTAT28200.021.2507551
Produced by FastQC (version 0.11.5)