##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937959_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2343452 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48363354572656 33.0 31.0 34.0 31.0 34.0 2 32.70466004850964 34.0 31.0 34.0 31.0 34.0 3 32.77510399188889 34.0 31.0 34.0 31.0 34.0 4 36.22962450265676 37.0 35.0 37.0 35.0 37.0 5 36.15482160505101 37.0 35.0 37.0 35.0 37.0 6 36.21147179460044 37.0 37.0 37.0 35.0 37.0 7 36.18187656499899 37.0 37.0 37.0 35.0 37.0 8 36.1741486490869 37.0 37.0 37.0 35.0 37.0 9 37.86914773590413 39.0 38.0 39.0 35.0 39.0 10-11 37.83994402274935 39.0 38.0 39.0 35.0 39.0 12-13 37.7602641317168 39.0 38.0 39.0 35.0 39.0 14-15 39.015691595134015 40.0 38.0 41.0 36.0 41.0 16-17 38.91870326339093 40.0 38.0 41.0 35.5 41.0 18-19 39.16550349655124 40.0 39.0 41.0 36.0 41.0 20-21 39.13406995321432 40.0 39.0 41.0 36.0 41.0 22-23 39.02689237927638 40.0 38.5 41.0 35.0 41.0 24-25 38.91476590943616 40.0 38.0 41.0 35.0 41.0 26-27 38.75052657361874 40.0 38.0 41.0 35.0 41.0 28-29 38.62893458026876 40.0 38.0 41.0 34.5 41.0 30-31 38.39331742233253 40.0 38.0 41.0 34.0 41.0 32-33 38.16471256932081 40.0 38.0 41.0 34.0 41.0 34-35 38.070942353417095 40.0 38.0 41.0 33.5 41.0 36-37 38.01813201209156 40.0 38.0 41.0 33.0 41.0 38-39 37.82499193497456 40.0 37.0 41.0 33.0 41.0 40-41 37.61009122439888 40.0 37.0 41.0 33.0 41.0 42-43 37.34915927443788 40.0 37.0 41.0 32.0 41.0 44-45 37.09134196049247 40.0 36.0 41.0 31.5 41.0 46-47 36.73267555725485 39.0 36.0 41.0 31.0 41.0 48-49 36.31793567779498 39.0 35.0 41.0 30.0 41.0 50-51 35.92855411589399 39.0 35.0 40.0 29.0 41.0 52-53 35.38973317994139 38.0 35.0 40.0 27.5 41.0 54-55 35.00468966294168 38.0 34.0 40.0 26.5 41.0 56-57 34.61277743260797 38.0 34.0 40.0 26.0 41.0 58-59 34.08841422824108 37.0 33.0 40.0 24.5 41.0 60-61 33.592875168768124 37.0 33.0 39.5 23.0 41.0 62-63 33.020199261602116 36.0 32.0 39.0 21.5 40.0 64-65 32.43839003316475 35.5 31.0 39.0 19.5 40.0 66-67 31.85629319482541 35.0 31.0 38.0 17.5 40.0 68-69 31.205985870416804 35.0 30.0 38.0 10.5 40.0 70-71 30.568595815062565 34.5 29.5 37.0 7.5 39.0 72-73 29.88544036745792 34.0 29.0 36.5 6.0 39.0 74-75 29.22429603849364 34.0 28.0 36.0 2.0 38.5 76-77 27.912063912552934 31.5 26.5 34.5 2.0 36.5 78-79 28.557402498536348 33.5 28.0 35.0 2.0 37.0 80-81 28.27834685754178 33.5 27.0 35.0 2.0 37.0 82-83 27.704400388828105 33.0 26.0 35.0 2.0 36.0 84-85 27.039369912419797 33.0 25.5 35.0 2.0 36.0 86-87 26.3097550109838 32.0 24.5 35.0 2.0 35.0 88-89 25.495022513795888 32.0 21.0 34.0 2.0 35.0 90-91 24.81130272777083 31.0 18.5 34.0 2.0 35.0 92-93 23.846205298849732 31.0 8.5 34.0 2.0 35.0 94-95 22.923150122127527 30.5 2.0 34.0 2.0 35.0 96-97 21.85789638533241 30.0 2.0 34.0 2.0 35.0 98-99 20.412727676948364 29.0 2.0 34.0 2.0 35.0 100-101 16.54029653690368 14.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0775859260804097 1101 2 0.09875386706291778 1101 3 0.05537500002798623 1101 4 0.00928918313312721 1101 5 -0.11598899658800121 1101 6 -0.006126460847092119 1101 7 -0.0019198840556811092 1101 8 0.020099169027844255 1101 9 -0.045323069490564194 1101 10-11 0.06561573033911827 1101 12-13 0.11420291215777212 1101 14-15 0.23484324386959088 1101 16-17 0.3014186202103417 1101 18-19 0.11698866811970987 1101 20-21 0.10580391875467399 1101 22-23 0.12812715217526005 1101 24-25 0.22674857449223396 1101 26-27 0.281107543533075 1101 28-29 0.26479329268578056 1101 30-31 0.2577646807219125 1101 32-33 0.19099495356958585 1101 34-35 0.17714190626975324 1101 36-37 0.26034135047834184 1101 38-39 0.3822488249954077 1101 40-41 0.5034914215653075 1101 42-43 0.6338950063683981 1101 44-45 0.41670318522690053 1101 46-47 0.5041642926735079 1101 48-49 0.5953310705403609 1101 50-51 0.6020517725970365 1101 52-53 0.6378686752728058 1101 54-55 0.8588866942343927 1101 56-57 0.8590552135413603 1101 58-59 1.1446475377657777 1101 60-61 1.0017630987064408 1101 62-63 1.0443046482275875 1101 64-65 1.3359240715253478 1101 66-67 1.452630724688401 1101 68-69 1.7962405952892233 1101 70-71 1.8656885629376916 1101 72-73 1.8735478287025344 1101 74-75 1.976719713806144 1101 76-77 1.8955495885856877 1101 78-79 2.017018475155009 1101 80-81 2.0637023985680933 1101 82-83 2.233863299160827 1101 84-85 2.3632662946869942 1101 86-87 2.701304545431462 1101 88-89 2.973186392411172 1101 90-91 3.2083988283061373 1101 92-93 3.318075264307943 1101 94-95 3.6611559026070886 1101 96-97 4.068723342029092 1101 98-99 4.424494592729449 1101 100-101 4.407936639338121 1102 1 0.09016869318686815 1102 2 0.08823038513506987 1102 3 0.14698457293652467 1102 4 0.06416537635498543 1102 5 0.005457953323997344 1102 6 0.017850797748067748 1102 7 0.09723011613274224 1102 8 0.09063586919705813 1102 9 0.11852753705995411 1102 10-11 0.16206932234673843 1102 12-13 0.21851396196689876 1102 14-15 0.3548190402615603 1102 16-17 0.3440077609021728 1102 18-19 0.246808427082307 1102 20-21 0.26069228915184084 1102 22-23 0.25930006146966633 1102 24-25 0.314381133762339 1102 26-27 0.3468234248308164 1102 28-29 0.24304054728625601 1102 30-31 0.3031900197985351 1102 32-33 0.22969717287745794 1102 34-35 0.16194493280421796 1102 36-37 0.2430596540211667 1102 38-39 0.2489345464683126 1102 40-41 0.3409839550961138 1102 42-43 0.3351487372476427 1102 44-45 0.3993659024375589 1102 46-47 0.42160916799053894 1102 48-49 0.48041739894064506 1102 50-51 0.461807338767386 1102 52-53 0.6003316491227366 1102 54-55 0.713589085954105 1102 56-57 0.7814682350636346 1102 58-59 0.9830362831351493 1102 60-61 0.8953730965206717 1102 62-63 0.8820270642168495 1102 64-65 0.9568720398920121 1102 66-67 1.0722569866865719 1102 68-69 1.3505493839594145 1102 70-71 1.4509335008129014 1102 72-73 1.5761704907790914 1102 74-75 1.8079399421434914 1102 76-77 1.811671815392998 1102 78-79 1.8823827257947237 1102 80-81 1.9712956564170696 1102 82-83 2.0573023831357595 1102 84-85 2.2453607358653507 1102 86-87 2.329690129917033 1102 88-89 2.3064620195945835 1102 90-91 2.7149237451150654 1102 92-93 2.8541368973024817 1102 94-95 3.165273059752476 1102 96-97 3.5556666778873662 1102 98-99 3.5714091508840546 1102 100-101 3.4852417998224574 1103 1 0.2630440204382438 1103 2 0.22368921162438227 1103 3 0.21843567301164057 1103 4 0.1277711031629778 1103 5 0.05179683990359507 1103 6 0.06965268577886974 1103 7 0.1719509953177365 1103 8 0.12926155925079996 1103 9 0.2706255921475744 1103 10-11 0.21035667640538946 1103 12-13 0.2893515796497681 1103 14-15 0.34611679708552856 1103 16-17 0.40008647807675857 1103 18-19 0.33113399753888473 1103 20-21 0.2876734361907225 1103 22-23 0.2904454595313197 1103 24-25 0.40097102853142275 1103 26-27 0.47862756442403764 1103 28-29 0.3896462029922816 1103 30-31 0.41075454027954805 1103 32-33 0.4890607555416935 1103 34-35 0.4013192901517897 1103 36-37 0.49487366584961734 1103 38-39 0.49731492560589885 1103 40-41 0.5589837381439438 1103 42-43 0.6430168540298951 1103 44-45 0.5842474356148273 1103 46-47 0.6788269296275615 1103 48-49 0.7365052773307497 1103 50-51 0.8125039101938754 1103 52-53 0.9515111214367238 1103 54-55 1.109108157690521 1103 56-57 1.1549746378872925 1103 58-59 1.274298899177218 1103 60-61 1.2817564765260698 1103 62-63 1.4174114615273723 1103 64-65 1.566499656991489 1103 66-67 1.5711426000231121 1103 68-69 1.5869233292801717 1103 70-71 1.7193871970215255 1103 72-73 1.850009933412565 1103 74-75 2.0377034551642694 1103 76-77 1.8363480735332658 1103 78-79 1.8861365452467602 1103 80-81 2.1752332015394735 1103 82-83 2.2436335305392596 1103 84-85 2.3554623661059324 1103 86-87 2.5505292061018814 1103 88-89 2.8766793181572297 1103 90-91 3.0121384165510356 1103 92-93 3.230839242573655 1103 94-95 3.3575911298626657 1103 96-97 3.375639221587729 1103 98-99 3.7924211962872256 1103 100-101 3.825890660003168 1104 1 0.19695181486378743 1104 2 0.1839501488821753 1104 3 0.19680520160708426 1104 4 0.13130536692457184 1104 5 -0.0351700046754857 1104 6 0.05446643287564257 1104 7 0.09692309997788584 1104 8 0.20237083596190075 1104 9 0.18879904842395234 1104 10-11 0.21591151076493276 1104 12-13 0.26905726619967396 1104 14-15 0.344238638976492 1104 16-17 0.3230762307829167 1104 18-19 0.26982606159760536 1104 20-21 0.36555886907984814 1104 22-23 0.38288813903830743 1104 24-25 0.41240747733188954 1104 26-27 0.4597007951890646 1104 28-29 0.4568327642068013 1104 30-31 0.41146115816869866 1104 32-33 0.5350181411131558 1104 34-35 0.46291995101405803 1104 36-37 0.4772056564773024 1104 38-39 0.5696583348541324 1104 40-41 0.7115745565310831 1104 42-43 0.8069125978394354 1104 44-45 0.770345222392379 1104 46-47 0.8716183168960114 1104 48-49 0.9780529786821504 1104 50-51 1.0815395705373092 1104 52-53 1.2586418022965304 1104 54-55 1.2229965139856418 1104 56-57 1.2835172819940013 1104 58-59 1.5318090762256205 1104 60-61 1.4919071348475512 1104 62-63 1.6388914923926663 1104 64-65 1.7286641389324302 1104 66-67 1.7204250503207312 1104 68-69 1.8048734733794127 1104 70-71 2.1073428369190594 1104 72-73 2.2647431779533775 1104 74-75 2.2514248877954763 1104 76-77 2.202983368173385 1104 78-79 2.20538659296669 1104 80-81 2.3161362675287656 1104 82-83 2.4683764514428965 1104 84-85 2.6054274568976297 1104 86-87 2.743437064153998 1104 88-89 3.0639788570994675 1104 90-91 3.365635358418981 1104 92-93 3.733317903119076 1104 94-95 4.039547745082906 1104 96-97 4.397395451546963 1104 98-99 4.369233624734022 1104 100-101 3.818152985942863 1105 1 0.16424205424236504 1105 2 0.16057303619518137 1105 3 0.1574304377139768 1105 4 0.13795574033670732 1105 5 0.05182134466852517 1105 6 0.0062812869928947634 1105 7 0.10525670887364669 1105 8 0.08280820863953409 1105 9 0.12566599385958455 1105 10-11 0.05358069356702799 1105 12-13 0.15778445839895028 1105 14-15 0.30920774091354986 1105 16-17 0.21028201779421352 1105 18-19 0.22516781411449216 1105 20-21 0.22885255148563743 1105 22-23 0.24141028273358955 1105 24-25 0.20137546098652592 1105 26-27 0.1697304657466745 1105 28-29 0.27644799890081373 1105 30-31 0.360454253075396 1105 32-33 0.42595724782996314 1105 34-35 0.409829192776499 1105 36-37 0.3928081010667057 1105 38-39 0.3194030638460035 1105 40-41 0.5442416300280897 1105 42-43 0.43485001811421853 1105 44-45 0.5381409935613277 1105 46-47 0.76121849085105 1105 48-49 0.7449798764805635 1105 50-51 0.8769733508829987 1105 52-53 0.944422079843001 1105 54-55 1.0463034835104423 1105 56-57 0.944642037620234 1105 58-59 1.0963567179624008 1105 60-61 1.205578638386271 1105 62-63 1.40480832865547 1105 64-65 1.4543670986732593 1105 66-67 1.6769126414943152 1105 68-69 1.826446503934772 1105 70-71 1.9766740419084385 1105 72-73 2.094540594480513 1105 74-75 2.1048382043746123 1105 76-77 2.121835645671762 1105 78-79 2.2649691008653114 1105 80-81 2.34337295761323 1105 82-83 2.5095037923456793 1105 84-85 2.498253915700044 1105 86-87 2.6201927224525825 1105 88-89 2.6020874367104554 1105 90-91 2.800749827049458 1105 92-93 3.1491501166669273 1105 94-95 3.3601495934554357 1105 96-97 3.721490128783202 1105 98-99 3.763452826124059 1105 100-101 3.5972165546866712 1106 1 0.07761317441393345 1106 2 0.029320139335183626 1106 3 8.943359644675297E-4 1106 4 0.058953163604286374 1106 5 -0.03863119396282144 1106 6 0.014680020623181633 1106 7 0.06793965294755111 1106 8 0.08792200122828575 1106 9 0.11989309197181086 1106 10-11 0.16896606432582928 1106 12-13 0.10409356416281668 1106 14-15 0.025980658513105936 1106 16-17 0.031156889153983514 1106 18-19 -0.044039251474345065 1106 20-21 -0.08876821320684058 1106 22-23 -0.063757126314826 1106 24-25 -0.04737712163780827 1106 26-27 -0.19971492795132662 1106 28-29 0.08171282100414601 1106 30-31 0.16862251448804244 1106 32-33 0.2477338773382698 1106 34-35 0.2096462487025832 1106 36-37 0.2907264862096426 1106 38-39 0.18415603533697578 1106 40-41 0.3593732554906026 1106 42-43 0.28933799074429345 1106 44-45 0.3087168500499047 1106 46-47 0.502975205674332 1106 48-49 0.5989706693756958 1106 50-51 0.7396520724152111 1106 52-53 0.753082414661101 1106 54-55 0.9716660329043592 1106 56-57 1.0143901777379156 1106 58-59 1.1863013732066037 1106 60-61 0.9618551029208504 1106 62-63 0.9047699329856442 1106 64-65 0.916445924688837 1106 66-67 1.2285612985296588 1106 68-69 1.2136800910133978 1106 70-71 1.1023497467783088 1106 72-73 1.3604705749193435 1106 74-75 1.6153803198090841 1106 76-77 1.564326198962199 1106 78-79 1.7513446281920295 1106 80-81 1.7347779248934394 1106 82-83 2.0168417842226845 1106 84-85 2.298052706678341 1106 86-87 2.4833443686546524 1106 88-89 2.6016339553721224 1106 90-91 2.6467744513414466 1106 92-93 2.5648254680216738 1106 94-95 2.8856463803958015 1106 96-97 3.2788624901856345 1106 98-99 3.508997946437038 1106 100-101 3.4942704971658323 1107 1 0.24343864383757108 1107 2 0.2069108423995374 1107 3 0.1953089180165719 1107 4 0.14918293099908908 1107 5 0.0935878198088389 1107 6 0.0983273043136208 1107 7 0.1604363052855433 1107 8 0.1550882745018498 1107 9 0.1736529076962583 1107 10-11 0.2513466116824077 1107 12-13 0.2646321385567987 1107 14-15 0.3844103244108936 1107 16-17 0.31730827936252837 1107 18-19 0.27497614065663356 1107 20-21 0.26276791703815405 1107 22-23 0.28254941834465086 1107 24-25 0.2728537247554925 1107 26-27 0.34194972959114267 1107 28-29 0.2848702551852398 1107 30-31 0.32199217146928305 1107 32-33 0.37443646187562507 1107 34-35 0.43514715300609197 1107 36-37 0.3893400324231848 1107 38-39 0.3866804965886743 1107 40-41 0.5644110322952187 1107 42-43 0.5938628875477647 1107 44-45 0.6730892088750124 1107 46-47 0.7622786034524722 1107 48-49 0.7718384813880661 1107 50-51 0.6243202744479959 1107 52-53 0.9105458433310005 1107 54-55 0.9267621072469652 1107 56-57 0.9624018166931663 1107 58-59 0.9943821233990846 1107 60-61 1.3135445853335739 1107 62-63 1.3105963865507633 1107 64-65 1.4058930822194569 1107 66-67 1.5890951103112094 1107 68-69 1.7721311787861787 1107 70-71 1.9685277483308 1107 72-73 1.9231356854040484 1107 74-75 1.8174177495951334 1107 76-77 1.8192658778079824 1107 78-79 1.890578910780551 1107 80-81 1.9346081506057011 1107 82-83 2.2383809261777827 1107 84-85 2.3819073912708717 1107 86-87 2.384470262089124 1107 88-89 2.6074374258739645 1107 90-91 2.7049515406856806 1107 92-93 2.9431406217453677 1107 94-95 3.022428708060378 1107 96-97 3.5751270210940014 1107 98-99 3.885079711599534 1107 100-101 3.8264632078788274 1108 1 0.15630580756035073 1108 2 0.17761302366636755 1108 3 0.08118461357027229 1108 4 0.05868923466931619 1108 5 0.08354458552204136 1108 6 0.07626290275126735 1108 7 0.1507309235533043 1108 8 0.14550675196519336 1108 9 0.08819146795455879 1108 10-11 0.14827749323266204 1108 12-13 0.12427256949045073 1108 14-15 0.27362620336051435 1108 16-17 0.2946096263764417 1108 18-19 0.27278140388954597 1108 20-21 0.2527329132651701 1108 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2.538638560962191 1108 90-91 2.5252227140337524 1108 92-93 2.7012828582730677 1108 94-95 3.0478100066869054 1108 96-97 3.1476377473605623 1108 98-99 3.2920071544009772 1108 100-101 3.155469064190516 1109 1 0.057323197330141795 1109 2 0.07023720149432933 1109 3 0.04397067290979351 1109 4 0.049246237087409384 1109 5 0.039255734665886166 1109 6 0.03544301907925984 1109 7 0.025123727466613843 1109 8 0.0432889031451964 1109 9 0.05417308637832008 1109 10-11 0.07653069631092535 1109 12-13 0.0738333952735033 1109 14-15 0.1908421624214185 1109 16-17 0.11732204852579997 1109 18-19 0.09088420895880489 1109 20-21 0.13586771016226606 1109 22-23 0.16296886660197885 1109 24-25 0.06403029174148855 1109 26-27 0.1248824331173708 1109 28-29 0.1910574545303021 1109 30-31 0.13104353490145115 1109 32-33 0.15732347600495444 1109 34-35 0.1743246441113513 1109 36-37 0.14649456447292408 1109 38-39 0.15122504366157585 1109 40-41 0.29493176316881886 1109 42-43 0.39972872922839287 1109 44-45 0.4518919649876807 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58-59 0.34955760096460864 2316 60-61 0.4568629212202069 2316 62-63 0.526376308494612 2316 64-65 0.802446440294986 2316 66-67 0.9896028650655246 2316 68-69 1.2548918237739564 2316 70-71 1.217939367678234 2316 72-73 1.2559957159013528 2316 74-75 1.6472746250816073 2316 76-77 1.8115946522862423 2316 78-79 1.8200582167460908 2316 80-81 1.846609159096527 2316 82-83 1.9836685846014888 2316 84-85 2.2420753512456244 2316 86-87 2.4058972960150733 2316 88-89 2.823481114421078 2316 90-91 3.0738897806492496 2316 92-93 3.317115008730596 2316 94-95 3.647764177617489 2316 96-97 4.105684244619784 2316 98-99 4.426722329037535 2316 100-101 4.545068957990775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 14.0 5 33.0 6 80.0 7 212.0 8 562.0 9 1066.0 10 1963.0 11 3184.0 12 4667.0 13 7299.0 14 9750.0 15 13498.0 16 17490.0 17 23896.0 18 24932.0 19 21580.0 20 21374.0 21 22696.0 22 24719.0 23 27664.0 24 30825.0 25 34787.0 26 39519.0 27 45794.0 28 55205.0 29 66378.0 30 79467.0 31 95100.0 32 114335.0 33 142806.0 34 185415.0 35 252861.0 36 344464.0 37 414163.0 38 207069.0 39 8573.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80140920317548 18.430546049161663 13.84265604757426 23.925388700088586 2 16.224485397455272 19.687433715487774 39.17056709454364 24.91751379251331 3 19.89366614121348 22.840101494682454 29.139053347865087 28.12717901623898 4 13.253119501852648 15.929697111423003 35.535543822381385 35.28163956434297 5 13.922725152135019 37.02016162796858 34.005658175131714 15.051455044764692 6 34.09636723943994 35.09007225238665 16.28682814924308 14.52673235893033 7 29.9310162956186 32.066242449173274 19.494062605080025 18.5086786501281 8 27.172866647777123 34.618434033953385 18.928305641942984 19.280393676326508 9 26.43642797036167 16.394148461329696 19.705630838609025 37.463792729699605 10-11 25.51025154344958 26.085428675304634 26.46713054075782 21.937189240487964 12-13 26.253407366568638 23.28001597643135 27.197122023408205 23.269454633591813 14-15 22.85448987220562 24.861955781471096 25.358210878652514 26.92534346767077 16-17 22.595923150105733 28.109542698024686 26.37697003757065 22.91756411429893 18-19 22.659542418620052 26.484882131146705 28.571718132054762 22.283857318178484 20-21 22.791740560506465 25.58987766764585 29.281077658087302 22.337304113760386 22-23 22.682949830655154 25.578134394563897 29.037126143029724 22.701789631751232 24-25 22.600217969977496 25.857819395572832 29.00114447039867 22.540818164051 26-27 22.09320685664344 26.302007763397324 28.88280820584952 22.72197717410972 28-29 22.310185999115834 26.659261636252847 28.735365605952246 22.29518675867908 30-31 22.293010481972747 26.181248858521535 28.701270604219758 22.82447005528596 32-33 22.04295202120632 26.04964812592705 28.866496945531633 23.040902907334992 34-35 22.856538064889758 26.205093527433444 28.58569484030207 22.352673567374726 36-37 22.939687079210383 25.75730129662144 28.553408637272653 22.74960298689553 38-39 22.378453348127366 26.136250886841673 28.629670921728557 22.85562484330241 40-41 22.9087039499627 25.999760158278466 28.54588061174149 22.545655280017343 42-43 22.442024513958415 25.990347874253516 28.43000821911495 23.137619392673127 44-45 22.48143376602982 26.29515564570125 28.756656554436415 22.466754033832515 46-47 22.8928520831662 26.53869163951299 27.906353533163898 22.66210274415691 48-49 22.6811771694065 26.50664489820999 27.946251939446594 22.865925992936916 50-51 22.83547945509445 26.855318564237713 27.81985720211039 22.489344778557445 52-53 23.080075888048913 26.5684767599251 27.758366717133526 22.593080634892456 54-55 22.553480933255727 26.744307116168798 27.986705082928943 22.715506867646532 56-57 22.75374591708 26.90055672157073 27.690716022926015 22.654981338423262 58-59 22.649130428103497 26.483943345116522 27.723759650293665 23.143166576486312 60-61 22.420770726261942 26.80225581748634 27.913479772574817 22.8634936836769 62-63 22.399071967337072 26.819559350906268 28.14036728723268 22.641001394523975 64-65 22.14466095315799 26.745096549875996 27.973391390137287 23.13685110682873 66-67 22.184540353867238 26.84211885086493 27.819502831189663 23.153837964078168 68-69 22.56583298839735 26.518137943100502 27.987351873109073 22.928677195393078 70-71 22.415714083326648 26.61759233814049 27.696108134495606 23.27058544403726 72-73 22.383864142270493 26.76008016380966 27.653356598163008 23.202699095756834 74-75 22.36341943423633 27.182293471340568 27.287224146259447 23.16706294816365 76-77 22.510595480513363 26.768139479707713 27.679658042921297 23.041606996857627 78-79 22.52028204546114 26.825192067087357 27.69083813109891 22.963687756352595 80-81 22.585955093937457 27.02757369217787 27.442027184308586 22.944444029576083 82-83 22.47898303515249 27.09355128403995 27.381380380553168 23.046085300254397 84-85 22.488518613033094 27.112844046876322 27.374327780902448 23.02430955918814 86-87 22.61321457619799 27.103028870239683 27.175872307630595 23.107884245931736 88-89 22.468478125432053 27.27339838836042 26.95000793700916 23.308115549198362 90-91 22.52093862030207 27.06852488076071 27.14907034531494 23.261466153622283 92-93 22.483029308899862 26.758452914759935 27.282124831231876 23.476392945108326 94-95 22.27784530637822 26.68448653620525 27.44012410753967 23.59754404987686 96-97 22.49034543088182 26.429900360579488 27.268493033774988 23.811261174763704 98-99 22.26806864403453 26.673663467397667 26.98312148061919 24.075146407948615 100-101 22.6029592242555 25.928203351295437 27.33045097574006 24.138386448709 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1177.0 1 779.5 2 591.0 3 795.0 4 618.5 5 566.0 6 1195.0 7 2365.5 8 3539.0 9 3934.5 10 3169.5 11 2429.0 12 2328.5 13 2416.0 14 2543.5 15 2676.5 16 2798.0 17 2896.0 18 3096.5 19 3431.0 20 3946.0 21 4570.0 22 5386.0 23 6620.5 24 8274.0 25 10440.0 26 13268.5 27 16215.5 28 18767.5 29 21445.5 30 24552.0 31 28762.0 32 35563.5 33 38530.5 34 38708.0 35 41524.5 36 46097.5 37 51050.0 38 54047.5 39 56653.0 40 60053.0 41 64853.0 42 68065.5 43 71106.0 44 74618.0 45 78455.0 46 82431.5 47 86544.5 48 88817.0 49 88363.0 50 88725.5 51 87161.0 52 86600.0 53 89308.0 54 91220.0 55 86142.5 56 77660.0 57 71140.0 58 65412.5 59 57661.0 60 46715.5 61 38072.5 62 32141.5 63 24729.5 64 17752.0 65 13360.0 66 9999.0 67 7695.5 68 5807.0 69 4200.5 70 3144.0 71 2252.5 72 1650.0 73 1358.0 74 1051.0 75 758.5 76 515.0 77 305.5 78 174.0 79 97.0 80 57.5 81 37.0 82 23.5 83 15.0 84 8.0 85 6.5 86 4.5 87 2.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01420980672955964 3 0.0033284231979148707 4 0.0020909325217670343 5 0.11811635143369695 6 0.0 7 0.0 8 1.7068836912383952E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.133604614047994E-5 18-19 0.0 20-21 0.0 22-23 3.84048830528639E-4 24-25 4.267209228095988E-4 26-27 0.006315469657582062 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01120142422375197 36-37 6.614174303548782E-4 38-39 0.007019559180217901 40-41 0.010241302147430372 42-43 0.005718060365648625 44-45 0.00362712784388159 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.133604614047994E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.066802307023997E-4 68-69 9.387860301811174E-4 70-71 0.0 72-73 2.133604614047994E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.920244152643195E-4 82-83 0.0020269243833455942 84-85 0.004011176674410229 86-87 0.002987046459667192 88-89 0.0 90-91 0.0023469650754527937 92-93 0.0 94-95 2.133604614047994E-5 96-97 8.534418456191976E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2343452.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.31433707406883 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48880256891613 38.73657860339195 2 13.596073258038238 13.953469720934148 3 4.514047622454375 6.949060838010683 4 2.0799350088441675 4.269219445439437 5 1.083516177882308 2.779995718852973 6 0.6714598246671384 2.0673309464798817 7 0.4541296929709254 1.6312354898317418 8 0.3339690398769566 1.3709919907639427 9 0.24671780855073372 1.1394144711133138 >10 1.3552542046097575 13.041017730376012 >50 0.11275907884108678 3.9871461216708317 >100 0.05652508477918594 5.3744195601332665 >500 0.0035714277008262268 1.2393006060698015 >1k 0.0029900324937144874 2.4808600652970667 >5k 2.4916937447620724E-4 0.9799586916348925 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9849 0.4202774368751739 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7935 0.33860305224941667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5209 0.22227892869152002 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3352 0.1430368533257775 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3259 0.13906834874364826 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3081 0.1314727163176374 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2696 0.11504396078946784 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.2672092280959886E-5 0.0 2 0.0 0.0 0.0 4.2672092280959886E-5 0.0 3 0.0 0.0 0.0 4.2672092280959886E-5 0.0 4 0.0 0.0 0.0 8.534418456191977E-5 0.0 5 0.0 0.0 0.0 8.534418456191977E-5 0.0 6 0.0 0.0 0.0 8.534418456191977E-5 0.0 7 0.0 0.0 0.0 8.534418456191977E-5 0.0 8 0.0 0.0 0.0 8.534418456191977E-5 0.0 9 0.0 0.0 0.0 8.534418456191977E-5 0.0 10-11 0.0 0.0 0.0 8.534418456191977E-5 0.0 12-13 0.0 0.0 0.0 8.534418456191977E-5 0.0 14-15 0.0 0.0 0.0 1.066802307023997E-4 0.0 16-17 0.0 0.0 0.0 1.7068836912383952E-4 0.0 18-19 0.0 0.0 0.0 2.987046459667192E-4 0.0 20-21 0.0 0.0 0.0 4.267209228095988E-4 0.0 22-23 0.0 0.0 0.0 7.680976610572778E-4 0.0 24-25 0.0 0.0 0.0 0.0010027941686025573 0.0 26-27 0.0 0.0 0.0 0.0016428755528169556 0.0 28-29 0.0 0.0 0.0 0.0048219464277484666 0.0 30-31 0.0 0.0 0.0 0.013399036976221404 0.0 32-33 0.0 0.0 0.0 0.034927107531965665 0.0 34-35 0.0 0.0 0.0 0.06629109535847118 0.0 36-37 0.0 0.0 0.0 0.12007926767862112 0.0 38-39 0.0 0.0 0.0 0.19964138373647083 0.0 40-41 0.0 0.0 0.0 0.28406811831434997 0.0 42-43 0.0 0.0 0.0 0.36853752498451003 0.0 44-45 0.0 0.0 0.0 0.4687529337063443 0.0 46-47 0.0 0.0 0.0 0.5866985967709174 0.0 48-49 0.0 0.0 0.0 0.7063084714344481 0.0 50-51 0.0 0.0 0.0 0.8423044295338671 0.0 52-53 0.0 0.0 0.0 0.9640905809037266 0.0 54-55 0.0 0.0 0.0 1.0989770646038408 0.0 56-57 0.0 0.0 0.0 1.2534927107531966 0.0 58-59 0.0 0.0 0.0 1.4139184416834651 0.0 60-61 0.0 0.0 0.0 1.5859083096218742 0.0 62-63 0.0 0.0 0.0 1.7555938845771109 0.0 64-65 0.0 0.0 0.0 1.9271356955465697 0.0 66-67 0.0 0.0 0.0 2.1249635153610997 0.0 68-69 0.0 0.0 0.0 2.3417164081022355 0.0 70-71 0.0 0.0 0.0 2.5696493890209826 0.0 72-73 0.0 0.0 0.0 2.7951287246335745 0.0 74-75 0.0 0.0 0.0 3.024853933428122 0.0 76-77 0.0 0.0 0.0 3.2681275315218743 0.0 78-79 4.2672092280959886E-5 0.0 0.0 3.544493337179511 0.0 80-81 4.2672092280959886E-5 0.0 0.0 3.82672655552578 0.0 82-83 4.2672092280959886E-5 0.0 0.0 4.110645321517146 0.0 84-85 4.2672092280959886E-5 0.0 0.0 4.399407369982402 0.0 86-87 4.2672092280959886E-5 0.0 0.0 4.69139116141487 0.0 88-89 4.2672092280959886E-5 0.0 0.0 5.001766624620432 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4780 0.0 48.058716 1 GTATCAA 8140 0.0 40.257587 1 ATCAACG 10065 0.0 32.26694 3 TCAACGC 10190 0.0 31.870445 4 TATCAAC 10345 0.0 31.63085 2 CAACGCA 10380 0.0 31.332212 5 AACGCAG 10640 0.0 30.442738 6 ACGCAGA 12415 0.0 25.898994 7 CGCAGAG 12435 0.0 25.78095 8 TGGTATC 2340 0.0 24.59362 2 ATACCGT 240 2.3101165E-10 23.74712 6 GTACATG 7790 0.0 23.689028 1 TAAGGTG 1405 0.0 23.350979 5 GCAGAGT 13575 0.0 23.33603 9 GTAAGGT 1250 0.0 23.203922 4 TACATGG 7835 0.0 22.885231 2 GAGTACT 8560 0.0 22.415504 12-13 ACCGTCG 155 3.912308E-5 21.44901 8 GTACTTT 8895 0.0 21.437815 14-15 GTGGTAT 2820 0.0 21.250755 1 >>END_MODULE