FastQCFastQC Report
Thu 26 May 2016
SRR937958_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937958_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351456
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98540.4190595103629411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78440.33358055604697684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51050.21709953322537187No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33870.14403841704884124No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31880.13557557530313136No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29900.1271552604003647No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27190.11563048596273967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.054.504661
GTATCAA72450.042.4320951
ATCAACG91150.033.269683
TCAACGC91450.033.0565874
TATCAAC92650.032.9348532
CAACGCA93550.032.3653455
AACGCAG97850.031.0821696
TAAGGTG12050.027.2174025
ACGCAGA114450.026.3158727
CGCAGAG115150.026.1553388
GTACATG74100.026.1550051
TACATGG73100.025.8780672
GTATACG1351.2928607E-524.7520561
GCAGAGT123900.023.694259
ACATGGG76950.023.5960543
GTAAGGT11550.023.457274
GAGTACA55850.023.0774361
TGGTATC17700.022.5565222
GAGTACT81750.022.31239312-13
CAGAGTA118450.021.09305610-11
Produced by FastQC (version 0.11.5)