FastQCFastQC Report
Thu 26 May 2016
SRR937958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351456
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97480.41455166501095486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79200.33681259611066505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52520.22335097913803192No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32770.13936046432508198No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32200.13693643427731583No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30960.1316631057523509No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26390.11222833852727841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.050.3289031
GTATCAA76700.040.6909871
TATCAAC96550.032.2268072
ATCAACG96000.032.161973
TCAACGC97350.031.9572144
CAACGCA99650.031.267285
AACGCAG102200.030.4871276
CGCAGAG121550.025.516548
ACGCAGA121750.025.4746237
GTACATG76850.024.1691341
TACATGG75850.023.7362352
GCAGAGT132350.023.0754729
TAAGGTG11500.022.715825
GAGTACT85150.021.78211212-13
ACATGGG80350.021.696053
GTACTTT87200.021.2155614-15
GGACCGA7250.020.9640666
TGGTATC21250.020.5663452
AGGACCG8800.020.5099435
CAGAGTA127150.020.41443610-11
Produced by FastQC (version 0.11.5)