##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937958_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2351456 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.500251758910224 33.0 31.0 34.0 31.0 34.0 2 32.724827085856596 34.0 31.0 34.0 31.0 34.0 3 32.69076138358532 34.0 31.0 34.0 31.0 34.0 4 36.21555836043711 37.0 35.0 37.0 35.0 37.0 5 36.17698481281385 37.0 35.0 37.0 35.0 37.0 6 36.237933858851704 37.0 37.0 37.0 35.0 37.0 7 36.19853188832791 37.0 37.0 37.0 35.0 37.0 8 36.190440731189526 37.0 37.0 37.0 35.0 37.0 9 37.90467608154267 39.0 38.0 39.0 35.0 39.0 10-11 37.86768304403739 39.0 38.0 39.0 35.0 39.0 12-13 37.80951206401481 39.0 38.0 39.0 35.0 39.0 14-15 39.102027212076266 40.0 38.5 41.0 36.0 41.0 16-17 39.03172587537254 40.0 38.0 41.0 36.0 41.0 18-19 39.01672006620579 40.0 38.5 41.0 36.0 41.0 20-21 39.014238199651615 40.0 38.5 41.0 35.5 41.0 22-23 39.0435704516691 40.0 39.0 41.0 35.0 41.0 24-25 38.992242040676075 40.0 38.5 41.0 35.0 41.0 26-27 38.81805464359104 40.0 38.0 41.0 35.0 41.0 28-29 38.74181655961243 40.0 38.0 41.0 35.0 41.0 30-31 38.53619969925017 40.0 38.0 41.0 34.0 41.0 32-33 38.33679048215234 40.0 38.0 41.0 34.0 41.0 34-35 38.13179408842862 40.0 38.0 41.0 34.0 41.0 36-37 38.055814780289325 40.0 38.0 41.0 33.5 41.0 38-39 38.00827210885511 40.0 38.0 41.0 33.0 41.0 40-41 37.888882249976184 40.0 37.5 41.0 33.0 41.0 42-43 37.699186801709246 40.0 37.0 41.0 33.0 41.0 44-45 37.46604997074154 40.0 37.0 41.0 33.0 41.0 46-47 37.12688968026619 40.0 36.5 41.0 31.5 41.0 48-49 36.76208464032497 40.0 36.0 41.0 31.0 41.0 50-51 36.4603060826994 39.0 35.5 41.0 30.0 41.0 52-53 35.99231072152743 39.0 35.0 40.0 29.0 41.0 54-55 35.67891701992298 39.0 35.0 40.0 28.0 41.0 56-57 35.394466874991494 38.0 35.0 40.0 28.0 41.0 58-59 35.002658990855025 38.0 34.0 40.0 27.5 41.0 60-61 34.57697549943524 37.5 34.0 40.0 26.0 41.0 62-63 34.13134755657771 37.0 33.5 40.0 26.0 41.0 64-65 33.68689377985385 36.0 33.0 39.0 25.0 40.5 66-67 33.159334471918676 36.0 32.5 39.0 23.5 40.0 68-69 32.617382804526216 35.0 32.0 38.5 22.0 40.0 70-71 32.01047414878271 35.0 31.0 37.5 20.5 39.5 72-73 31.528726031871315 35.0 31.0 37.0 19.5 39.0 74-75 30.974114761237296 34.0 30.0 36.0 17.5 39.0 76-77 29.758954026781705 33.5 28.5 35.0 17.0 37.0 78-79 30.450063492576515 34.0 30.0 35.0 16.5 37.0 80-81 30.25981306900916 34.0 30.0 35.0 12.0 37.0 82-83 29.80291870228488 34.0 30.0 35.0 7.0 36.5 84-85 29.29376139719391 34.0 29.0 35.0 4.0 36.0 86-87 28.748155398187336 34.0 29.0 35.0 2.0 36.0 88-89 28.110794546017445 33.0 27.5 35.0 2.0 35.0 90-91 27.567299792128793 33.0 26.5 35.0 2.0 35.0 92-93 26.9152155090293 33.0 25.5 35.0 2.0 35.0 94-95 26.15228075711389 32.0 24.5 34.0 2.0 35.0 96-97 25.31103558816325 32.0 21.5 34.0 2.0 35.0 98-99 24.251845877617953 31.5 9.5 34.0 2.0 35.0 100-101 20.705353406570225 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09263451181476512 1101 2 0.036579133405055586 1101 3 0.21431388924352746 1101 4 0.10541459296605638 1101 5 0.040763067336534675 1101 6 0.1190895843106361 1101 7 0.09987635836220932 1101 8 0.1466046360888953 1101 9 0.08089388238666118 1101 10-11 0.16035914166906196 1101 12-13 0.13816659643077855 1101 14-15 0.23061731895045767 1101 16-17 0.25763541143074775 1101 18-19 0.3100051958759664 1101 20-21 0.2611774739767796 1101 22-23 0.1803041333848583 1101 24-25 0.22788477360699488 1101 26-27 0.34036618444211797 1101 28-29 0.24060095312404428 1101 30-31 0.268092938381713 1101 32-33 0.2920697406383894 1101 34-35 0.2948235808398394 1101 36-37 0.3340966789873008 1101 38-39 0.4472376504741007 1101 40-41 0.29133191755226306 1101 42-43 0.3213134945312248 1101 44-45 0.34854459418024675 1101 46-47 0.48252481104713496 1101 48-49 0.5284384627856653 1101 50-51 0.5584594576635808 1101 52-53 0.4938154420397325 1101 54-55 0.5186624337418024 1101 56-57 0.6020725130967293 1101 58-59 0.6266483252522974 1101 60-61 0.6494408028803988 1101 62-63 0.6063289222443728 1101 64-65 0.5792767648324855 1101 66-67 0.6463351549220135 1101 68-69 0.8323010167421003 1101 70-71 0.9666471140327104 1101 72-73 0.8922324454572141 1101 74-75 1.072693275940015 1101 76-77 1.1114747019719822 1101 78-79 1.2199915118674838 1101 80-81 1.278248153813763 1101 82-83 1.4263647436614093 1101 84-85 1.3851044109394124 1101 86-87 1.5700844419402955 1101 88-89 1.8484026667301947 1101 90-91 2.0394344091896066 1101 92-93 2.370107440654813 1101 94-95 2.4006687464356844 1101 96-97 2.680213519834762 1101 98-99 2.813792639234123 1101 100-101 3.0364285067444285 1102 1 0.09589452315282188 1102 2 0.1084113557538231 1102 3 0.22741621286272817 1102 4 0.07349143581623707 1102 5 -0.019201519363981845 1102 6 -0.022825523586462282 1102 7 0.040150647206246504 1102 8 0.05714233214279574 1102 9 0.04681146093939503 1102 10-11 0.13403079102364757 1102 12-13 0.06723943512964325 1102 14-15 0.1382841361940308 1102 16-17 0.14620690154919203 1102 18-19 0.17141259086563565 1102 20-21 0.15760135777747308 1102 22-23 0.1923979543143659 1102 24-25 0.10519648102539492 1102 26-27 0.17175577467244807 1102 28-29 0.06850264048117083 1102 30-31 0.10998121543639172 1102 32-33 0.11095611564654462 1102 34-35 0.09187961037850556 1102 36-37 0.09425443495631214 1102 38-39 0.08954445027975311 1102 40-41 0.19160841599509126 1102 42-43 0.199913530277243 1102 44-45 0.15782102735885672 1102 46-47 0.10805113705168168 1102 48-49 0.12291352737803862 1102 50-51 0.2657918064884157 1102 52-53 0.31343731911523776 1102 54-55 0.19572135964644843 1102 56-57 0.2533606068144323 1102 58-59 0.21545446793692946 1102 60-61 0.33145479672167966 1102 62-63 0.3466209774570359 1102 64-65 0.3975947440136238 1102 66-67 0.34605590514479445 1102 68-69 0.48222560032017014 1102 70-71 0.5940203532320112 1102 72-73 0.49106496256134236 1102 74-75 0.62364159052375 1102 76-77 0.561685466983711 1102 78-79 0.6734106740371004 1102 80-81 0.8058416917184061 1102 82-83 0.8790822089188808 1102 84-85 0.9547784353994118 1102 86-87 1.129013012607146 1102 88-89 1.2254478786192031 1102 90-91 1.2187463367147693 1102 92-93 1.3558171377648875 1102 94-95 1.3865488556529968 1102 96-97 1.556776288436449 1102 98-99 1.8276629903854378 1102 100-101 2.213974141571118 1103 1 0.24270403315086497 1103 2 0.2315296095390167 1103 3 0.27143851176948175 1103 4 0.15919667337967525 1103 5 0.10671437481136792 1103 6 0.10204058933771876 1103 7 0.11838865903170159 1103 8 0.07147005725388311 1103 9 0.1668176728429529 1103 10-11 0.22567692262921923 1103 12-13 0.2446041263957497 1103 14-15 0.3287349331218792 1103 16-17 0.30663311730474163 1103 18-19 0.4145617975918512 1103 20-21 0.34553085267206285 1103 22-23 0.4076080590108191 1103 24-25 0.43569982030419396 1103 26-27 0.40298627207387483 1103 28-29 0.37808115216692784 1103 30-31 0.38472168195339407 1103 32-33 0.43053914162006635 1103 34-35 0.48672563358027077 1103 36-37 0.5486426195503356 1103 38-39 0.3077893286946889 1103 40-41 0.393780755210706 1103 42-43 0.3603378428760635 1103 44-45 0.4478723897526322 1103 46-47 0.45140002298180093 1103 48-49 0.43637409815757877 1103 50-51 0.3707493775123183 1103 52-53 0.5740765361717877 1103 54-55 0.43930829242010105 1103 56-57 0.49261024540497544 1103 58-59 0.6101421484989231 1103 60-61 0.6703247936081098 1103 62-63 0.6318965583575675 1103 64-65 0.5965598835112118 1103 66-67 0.6076467005653399 1103 68-69 0.8729996293867046 1103 70-71 1.0577751139785647 1103 72-73 0.8301922662374075 1103 74-75 0.8269927450166819 1103 76-77 0.5892511696634308 1103 78-79 0.8589125022094564 1103 80-81 0.9216659070278546 1103 82-83 0.9695932978518726 1103 84-85 1.1578201366083718 1103 86-87 1.1992284575613823 1103 88-89 1.1919013156060636 1103 90-91 1.326116349207087 1103 92-93 1.5387215376348635 1103 94-95 1.685188783728897 1103 96-97 1.911447053741444 1103 98-99 2.3035591297939533 1103 100-101 2.686446882657304 1104 1 -0.015801178990436426 1104 2 -0.0339130285791569 1104 3 0.058053374244195766 1104 4 -2.8616496000921643E-4 1104 5 -0.09357784315860584 1104 6 0.031220691077898266 1104 7 0.013305372696251538 1104 8 -0.012163933768874813 1104 9 -0.06714235355546094 1104 10-11 0.003974857133719922 1104 12-13 -0.026252574191325095 1104 14-15 0.06331465265162706 1104 16-17 0.05684610023871528 1104 18-19 0.11931313723636805 1104 20-21 0.021317917965056665 1104 22-23 0.017537453324585783 1104 24-25 -0.07532589473670015 1104 26-27 -0.08008945667424427 1104 28-29 -0.09208250815694896 1104 30-31 -0.05006304901387182 1104 32-33 0.030299434858768848 1104 34-35 0.049606810992536055 1104 36-37 0.06274397437697132 1104 38-39 0.08586415494298194 1104 40-41 0.07026043936620141 1104 42-43 0.13022848237268647 1104 44-45 0.14036108626429922 1104 46-47 0.16031374797898224 1104 48-49 2.55946679317276E-6 1104 50-51 0.04032722063842442 1104 52-53 0.08854872360893751 1104 54-55 0.22526635509128567 1104 56-57 0.40932133472810506 1104 58-59 0.4406208432992358 1104 60-61 0.4559191640694209 1104 62-63 0.24717914615114012 1104 64-65 0.19970777010478713 1104 66-67 0.36329341133392745 1104 68-69 0.4666760671649044 1104 70-71 0.6660218319109674 1104 72-73 0.6012796837043233 1104 74-75 0.5623623567357434 1104 76-77 0.5755403128130112 1104 78-79 0.6764662524532241 1104 80-81 0.9829907112111478 1104 82-83 0.8710386788203408 1104 84-85 0.9840261795411465 1104 86-87 1.1698286187705627 1104 88-89 1.2272688905551021 1104 90-91 1.1020092563076673 1104 92-93 1.3302066215720316 1104 94-95 1.36929523516282 1104 96-97 1.8159400981718257 1104 98-99 2.0484339023838984 1104 100-101 2.419202522461781 1105 1 0.020574788241724207 1105 2 0.06047459780868536 1105 3 0.1531465142163526 1105 4 0.047650133861786514 1105 5 0.06406816683584537 1105 6 0.03971103815577948 1105 7 0.02856290864264821 1105 8 -0.011252594643195835 1105 9 0.0032717298527771277 1105 10-11 -0.008951787200096817 1105 12-13 0.054422599699066154 1105 14-15 0.05704210622952388 1105 16-17 0.06477081854653477 1105 18-19 0.17212713601776386 1105 20-21 0.15688483249230245 1105 22-23 0.06054768405449096 1105 24-25 -0.01837824749836159 1105 26-27 0.0946256324083734 1105 28-29 0.12124801008148722 1105 30-31 0.12402629910550189 1105 32-33 -0.03783108115265321 1105 34-35 0.1337158612964302 1105 36-37 0.2619278578369162 1105 38-39 0.25552825392296796 1105 40-41 0.25945569455681294 1105 42-43 0.2892664680591963 1105 44-45 0.29205990382005353 1105 46-47 0.20341123273171746 1105 48-49 0.31038799780560566 1105 50-51 0.4040144277545963 1105 52-53 0.49340651956013915 1105 54-55 0.652347393983085 1105 56-57 0.5043353980235494 1105 58-59 0.6049025086801478 1105 60-61 0.6103316426299799 1105 62-63 0.6980759226413014 1105 64-65 0.7729377313441717 1105 66-67 0.8000424221360305 1105 68-69 0.7454500602813141 1105 70-71 0.767946187631793 1105 72-73 0.7975044117901682 1105 74-75 0.7688306027089205 1105 76-77 0.5928824630653082 1105 78-79 0.9099118242115267 1105 80-81 1.0142699299996956 1105 82-83 1.001909012904946 1105 84-85 1.1057047416366075 1105 86-87 1.335119403458254 1105 88-89 1.5453974927195588 1105 90-91 1.4354017449107097 1105 92-93 1.6764476347556538 1105 94-95 1.7181724855423681 1105 96-97 1.7636942509587534 1105 98-99 1.9835945072012926 1105 100-101 2.279530406202884 1106 1 0.06120699105110816 1106 2 0.06780896904235334 1106 3 0.13664973669663283 1106 4 0.046368540776420275 1106 5 0.019028451365805665 1106 6 0.002174378178786185 1106 7 0.012760553647474637 1106 8 0.04265373570031983 1106 9 2.2030966368191685E-4 1106 10-11 -0.02395229800453791 1106 12-13 0.003105335153612998 1106 14-15 -0.026024709368030585 1106 16-17 0.02059319164557394 1106 18-19 -0.004286901363620643 1106 20-21 0.04297637407976396 1106 22-23 -0.04089180021289707 1106 24-25 0.06211783016835426 1106 26-27 0.037915138380711255 1106 28-29 -0.04673495382652959 1106 30-31 0.012387611285760158 1106 32-33 0.18283728864804516 1106 34-35 0.016524763073661575 1106 36-37 0.04414451494933758 1106 38-39 0.012336802040245232 1106 40-41 -0.02556962335531665 1106 42-43 -0.11068416549105109 1106 44-45 0.11715146679002686 1106 46-47 -0.1241265065530186 1106 48-49 0.039077185348219245 1106 50-51 0.19807054768400434 1106 52-53 -0.004350692540455725 1106 54-55 0.0948572867888231 1106 56-57 0.1551717936380328 1106 58-59 0.11360926382289449 1106 60-61 0.1663904791566111 1106 62-63 0.2465846960895135 1106 64-65 0.12390187898125049 1106 66-67 0.2552491995098052 1106 68-69 -0.0332035236822108 1106 70-71 0.10847592408723727 1106 72-73 0.2783194622274401 1106 74-75 0.3518417558949345 1106 76-77 0.4868867219186015 1106 78-79 0.6803423050838475 1106 80-81 0.8146959171505053 1106 82-83 0.8348253024078645 1106 84-85 0.681521358427716 1106 86-87 0.8320971720947234 1106 88-89 1.1846595007817804 1106 90-91 1.1039667034540912 1106 92-93 1.1851426804276812 1106 94-95 1.4827405095912454 1106 96-97 1.5332171629989055 1106 98-99 1.857761649883404 1106 100-101 2.034887234504371 1107 1 0.1169906141035355 1107 2 0.14051441275702814 1107 3 0.18654797387677746 1107 4 0.11668315441074384 1107 5 0.13328749880002277 1107 6 0.087728827249272 1107 7 0.07053535643318298 1107 8 0.07743992687626644 1107 9 0.07675351559133503 1107 10-11 0.030889350824509165 1107 12-13 0.04295475085000788 1107 14-15 0.23703567483903498 1107 16-17 0.18470779755003264 1107 18-19 0.14431535032893095 1107 20-21 0.18571494930160526 1107 22-23 0.14814355404094925 1107 24-25 0.1826977672275305 1107 26-27 0.2364109399962544 1107 28-29 0.211874838593765 1107 30-31 0.12004173775783045 1107 32-33 0.044632771283396266 1107 34-35 0.06671254117397751 1107 36-37 0.19827789455219857 1107 38-39 0.14152903961345942 1107 40-41 0.28986987278920395 1107 42-43 0.07854320184107166 1107 44-45 0.08532235925201093 1107 46-47 0.16264276444692172 1107 48-49 0.10203017380358403 1107 50-51 0.1238717602856596 1107 52-53 0.04083520654535988 1107 54-55 0.16626024864194733 1107 56-57 0.21692476202864697 1107 58-59 0.23064481214563415 1107 60-61 0.2434902675721773 1107 62-63 0.27241666367672224 1107 64-65 0.2786242905668317 1107 66-67 0.4897607338628802 1107 68-69 0.6086909340884858 1107 70-71 0.7135018150155901 1107 72-73 0.5586786212512713 1107 74-75 0.752365248510035 1107 76-77 0.7297035825582228 1107 78-79 0.8382740076354445 1107 80-81 0.985710412287677 1107 82-83 1.1138994951100507 1107 84-85 1.1658758696660314 1107 86-87 1.3186523916844983 1107 88-89 1.3956322053209398 1107 90-91 1.4755356106570865 1107 92-93 1.6726952167683145 1107 94-95 1.5476720139623197 1107 96-97 1.636517896595766 1107 98-99 1.7335383982919943 1107 100-101 2.1860160822696635 1108 1 0.08533167807811992 1108 2 0.037836286551559795 1108 3 0.12197187331885573 1108 4 0.04835008765125082 1108 5 0.047798367172134704 1108 6 0.05701183771464713 1108 7 0.03765551223775532 1108 8 0.053548952439129494 1108 9 0.0413614482014637 1108 10-11 0.04220211720232925 1108 12-13 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975.0 10 1623.0 11 2676.0 12 4161.0 13 6130.0 14 8337.0 15 11220.0 16 14729.0 17 20419.0 18 20788.0 19 16201.0 20 15071.0 21 15233.0 22 16107.0 23 17499.0 24 20097.0 25 23324.0 26 27680.0 27 32775.0 28 39518.0 29 48691.0 30 59498.0 31 72908.0 32 91451.0 33 119550.0 34 165913.0 35 247273.0 36 377527.0 37 516773.0 38 316969.0 39 19522.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.754422791666094 18.458648598995687 13.917079460555504 23.869849148782713 2 16.267982876792438 19.667445703142445 39.131377873804595 24.93319354626052 3 20.14452344518852 22.90144999774594 28.98583515430096 27.96819140276458 4 13.264972850863465 15.945950083692829 35.58833335601432 35.200743709429396 5 13.95114246577323 36.975498937038395 34.01783325940728 15.055525337781086 6 34.01501201386379 35.14452784452147 16.291224563567162 14.549235578047586 7 29.900920961310778 32.05061034524992 19.51935311568662 18.529115577752677 8 27.102785853166466 34.71603928126111 18.938808872135173 19.242365993437247 9 26.435791271450537 16.325799844862075 19.70710912728114 37.53129975640624 10-11 25.513554155382877 26.113565382469417 26.413762366805926 21.95911809534178 12-13 26.28237568553271 23.278194446334528 27.205888606888667 23.2335412612441 14-15 22.886990018099425 24.826490480791474 25.389014295823525 26.897505205285576 16-17 22.59302802030487 28.120476870602058 26.363756405341288 22.922738703751783 18-19 22.654644611678894 26.441596185512296 28.5831416790278 22.32061752378101 20-21 22.78435148265585 25.577067995318647 29.277605024291333 22.36097549773417 22-23 22.699196306287746 25.604630833966453 29.03048771282297 22.665685146922833 24-25 22.582700203919767 25.87510073721568 29.01990928866236 22.522289770202196 26-27 22.107623718955146 26.241549559681705 28.923954225443477 22.72687249591967 28-29 22.274658098312802 26.706467750554076 28.736093382639215 22.282780768493907 30-31 22.30991270289321 26.175289528493558 28.73625982238638 22.778537946226855 32-33 22.041596680737214 26.09058438596637 28.847939633374736 23.019879299921683 34-35 22.843613345690926 26.25468354365771 28.553017352664828 22.348685757986534 36-37 22.944436292804085 25.70843779837662 28.594224810899664 22.752901097919633 38-39 22.397808712363297 26.083451920322304 28.66398703671053 22.854752330603866 40-41 22.888753951956545 26.00605940084457 28.599414903063412 22.505771744135473 42-43 22.436242078909334 26.01384430978791 28.458716751410673 23.09119685989208 44-45 22.444208727472855 26.288725125137212 28.806036358161446 22.461029789228483 46-47 22.876185012372417 26.526691870096936 27.98003912138282 22.617083996147826 48-49 22.681957370567655 26.524011455907747 27.943454476290274 22.850576697234324 50-51 22.812249091626636 26.893103677040948 27.82027390689003 22.474373324442386 52-53 23.01378380033477 26.525395329532003 27.839240878842723 22.621579991290503 54-55 22.57401371745846 26.691122436481905 28.039861260427585 22.69500258563205 56-57 22.718796354258807 26.885491372154103 27.73762298762979 22.6580892859573 58-59 22.61388263271777 26.45526856551855 27.81102431854987 23.119824483213804 60-61 22.374690404583372 26.79558962617204 27.9789840847543 22.850735884490287 62-63 22.336182348298244 26.860315481131693 28.153811936094066 22.649690234476 64-65 22.10366683450594 26.75361563218704 28.06627042989535 23.076447103411674 66-67 22.171019827106864 26.820252588559622 27.878811075282194 23.12991650905132 68-69 22.533812072790738 26.5629378941446 28.021036354968608 22.882213678096054 70-71 22.359933590082058 26.66571264782331 27.74115271559408 23.23320104650055 72-73 22.364462698855515 26.75833611175374 27.7161044051005 23.161096784290244 74-75 22.41030663554836 27.192705285576256 27.329429085638857 23.06755899323653 76-77 22.489087612100757 26.79086914660534 27.66420039328824 23.05584284800566 78-79 22.53388964114149 26.848237857735803 27.668516867846986 22.949355633275722 80-81 22.564074758048484 27.04715489681378 27.472870491749248 22.915899853388492 82-83 22.496381459549212 27.074762756218924 27.409091015409125 23.019764768822736 84-85 22.462043702026342 27.161120468597794 27.40931773183596 22.96751809753991 86-87 22.529192774804184 27.17932601242304 27.261701137771865 23.029780075000915 88-89 22.420272218748014 27.304887251698894 27.048556542344322 23.226283987208767 90-91 22.457878506544958 27.186624041562453 27.12136374249209 23.234133709400503 92-93 22.46103605179217 26.871659090092376 27.298908560788266 23.368396297327187 94-95 22.23864233673492 26.79784660711764 27.45065602019518 23.512855035952253 96-97 22.490610041132104 26.43564731178406 27.357164606659385 23.716578040424455 98-99 22.26932077088523 26.71306352137668 27.108563749427216 23.909051958310872 100-101 22.413833431998967 26.265578112560473 27.423665972604976 23.89692248283558 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1206.0 1 809.0 2 644.5 3 877.5 4 673.5 5 589.5 6 1227.5 7 2445.0 8 3750.0 9 4218.0 10 3370.5 11 2487.5 12 2363.0 13 2458.5 14 2536.5 15 2614.0 16 2719.0 17 2909.5 18 3153.0 19 3460.5 20 3937.5 21 4489.5 22 5211.0 23 6313.5 24 7939.5 25 10214.5 26 12953.5 27 16017.5 28 18744.0 29 21483.0 30 24646.5 31 28587.0 32 35691.0 33 38951.0 34 38996.0 35 41892.0 36 46335.0 37 51114.0 38 54509.5 39 57444.5 40 60791.5 41 65795.0 42 68960.5 43 71353.0 44 74596.0 45 79211.0 46 83335.5 47 87357.0 48 89420.5 49 88541.0 50 89237.5 51 87877.5 52 87130.5 53 90213.0 54 92208.5 55 86450.0 56 77905.5 57 71284.0 58 65436.5 59 57597.0 60 46266.0 61 37544.5 62 31582.0 63 23939.5 64 17256.0 65 13186.5 66 9709.5 67 7455.5 68 5658.0 69 3993.0 70 2924.5 71 2107.5 72 1583.0 73 1330.5 74 996.0 75 696.5 76 487.0 77 275.5 78 150.5 79 94.0 80 57.0 81 32.5 82 20.0 83 13.0 84 9.5 85 4.5 86 2.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01109950600819237 3 0.006038811697943742 4 0.0 5 2.976879006028605E-4 6 2.5516105765959477E-4 7 0.0 8 1.7010737177306314E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1263421471632893E-5 18-19 0.0 20-21 0.0 22-23 2.1263421471632893E-5 24-25 0.0017436005606738974 26-27 0.023092075718193324 28-29 5.315855367908224E-4 30-31 1.4884395030143025E-4 32-33 0.003848679286365554 34-35 0.017223371392022645 36-37 0.008122627002163766 38-39 0.02166742647959392 40-41 0.02396387599853027 42-43 0.019307186696242668 44-45 0.010078861777553993 46-47 0.002402766626294517 48-49 2.3389763618796185E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4884395030143025E-4 68-69 1.2758052882979738E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4884395030143025E-4 82-83 0.0010419076521100117 84-85 0.004273947715798212 86-87 7.654831729787843E-4 88-89 6.379026441489869E-5 90-91 0.004380264823156376 92-93 8.292734373936829E-4 94-95 1.7010737177306314E-4 96-97 3.402147435461263E-4 98-99 1.4884395030143025E-4 100-101 0.003402147435461263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2351456.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.144992176402816 #Duplication Level Percentage of deduplicated Percentage of total 1 75.11412535419376 38.4171135357758 2 13.85003288614189 14.167196472092968 3 4.622511089008092 7.092548804479623 4 2.053267737946062 4.200574495732465 5 1.1131040932773248 2.846485007109537 6 0.6705247958161142 2.0576391261659555 7 0.4599075380842575 1.646537720603162 8 0.31795046102430213 1.300925907325728 9 0.253366748722936 1.1662596343075664 >10 1.3677332117738674 13.044709724265088 >50 0.11477018280148683 4.044855070839692 >100 0.05628976799125006 5.451866248738278 >500 0.003249729812086309 1.1677135690562315 >1k 0.002916424190333601 2.418786544720842 >5k 2.499792163143086E-4 0.9767881387871186 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9748 0.41455166501095486 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7920 0.33681259611066505 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5252 0.22335097913803192 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3277 0.13936046432508198 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3220 0.13693643427731583 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3096 0.1316631057523509 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2639 0.11222833852727841 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.252684294326579E-5 0.0 12-13 0.0 0.0 0.0 8.505368588653159E-5 4.252684294326579E-5 14-15 0.0 0.0 0.0 1.0631710735816446E-4 8.505368588653157E-5 16-17 0.0 0.0 0.0 1.7010737177306317E-4 1.7010737177306317E-4 18-19 0.0 0.0 0.0 2.551610576595947E-4 1.7010737177306317E-4 20-21 0.0 0.0 0.0 5.103221153191894E-4 2.1263421471632896E-4 22-23 0.0 0.0 0.0 8.0801001592205E-4 2.1263421471632896E-4 24-25 0.0 0.0 0.0 0.0010206442306383789 2.1263421471632896E-4 26-27 0.0 0.0 0.0 0.0015309663459575685 2.1263421471632896E-4 28-29 0.0 0.0 0.0 0.004401528244628009 2.1263421471632896E-4 30-31 0.0 0.0 0.0 0.013204584733884028 2.1263421471632896E-4 32-33 0.0 0.0 0.0 0.0353610699073255 2.1263421471632896E-4 34-35 0.0 0.0 0.0 0.06721367527183159 2.1263421471632896E-4 36-37 0.0 0.0 0.0 0.11941537498469035 2.1263421471632896E-4 38-39 0.0 0.0 0.0 0.19843024917327817 2.1263421471632896E-4 40-41 0.0 0.0 0.0 0.28607807247934897 2.1263421471632896E-4 42-43 0.0 0.0 0.0 0.37217366601799057 2.1263421471632896E-4 44-45 0.0 0.0 0.0 0.4763006409645768 2.1263421471632896E-4 46-47 0.0 0.0 0.0 0.594801688825987 2.1263421471632896E-4 48-49 0.0 0.0 0.0 0.719447865492699 2.1263421471632896E-4 50-51 0.0 0.0 0.0 0.8526844644339506 2.1263421471632896E-4 52-53 0.0 0.0 0.0 0.9786702366533755 2.1263421471632896E-4 54-55 0.0 0.0 0.0 1.1148837146006558 2.1263421471632896E-4 56-57 0.0 0.0 0.0 1.2689372031626363 2.1263421471632896E-4 58-59 0.0 0.0 0.0 1.428072649456337 2.1263421471632896E-4 60-61 0.0 0.0 0.0 1.6013907978716166 2.1263421471632896E-4 62-63 0.0 0.0 0.0 1.7654593579467361 2.1263421471632896E-4 64-65 0.0 0.0 0.0 1.9395217261135227 2.1263421471632896E-4 66-67 0.0 0.0 0.0 2.139461678211287 2.1263421471632896E-4 68-69 0.0 0.0 0.0 2.354583713239797 2.1263421471632896E-4 70-71 0.0 0.0 0.0 2.577828375270471 2.1263421471632896E-4 72-73 0.0 0.0 0.0 2.806112468189921 2.1263421471632896E-4 74-75 0.0 0.0 0.0 3.029484710749425 2.1263421471632896E-4 76-77 0.0 0.0 0.0 3.2715687642039657 2.1263421471632896E-4 78-79 0.0 0.0 0.0 3.54391066641264 2.1263421471632896E-4 80-81 0.0 0.0 0.0 3.8326466665759424 2.1263421471632896E-4 82-83 0.0 0.0 0.0 4.118597158526462 2.1263421471632896E-4 84-85 0.0 0.0 0.0 4.4073969489541795 2.1263421471632896E-4 86-87 0.0 0.0 0.0 4.705935386415906 2.551610576595947E-4 88-89 0.0 0.0 0.0 5.023823537416817 2.551610576595947E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4540 0.0 50.328903 1 GTATCAA 7670 0.0 40.690987 1 TATCAAC 9655 0.0 32.226807 2 ATCAACG 9600 0.0 32.16197 3 TCAACGC 9735 0.0 31.957214 4 CAACGCA 9965 0.0 31.26728 5 AACGCAG 10220 0.0 30.487127 6 CGCAGAG 12155 0.0 25.51654 8 ACGCAGA 12175 0.0 25.474623 7 GTACATG 7685 0.0 24.169134 1 TACATGG 7585 0.0 23.736235 2 GCAGAGT 13235 0.0 23.075472 9 TAAGGTG 1150 0.0 22.71582 5 GAGTACT 8515 0.0 21.782112 12-13 ACATGGG 8035 0.0 21.69605 3 GTACTTT 8720 0.0 21.21556 14-15 GGACCGA 725 0.0 20.964066 6 TGGTATC 2125 0.0 20.566345 2 AGGACCG 880 0.0 20.509943 5 CAGAGTA 12715 0.0 20.414436 10-11 >>END_MODULE