FastQCFastQC Report
Thu 26 May 2016
SRR937957_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937957_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347679
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99060.4219486565241671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79910.3403787315046052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50670.21583018802826112No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33580.1430348867967043No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32970.14043657586918823No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30150.12842471223706478No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27680.11790368274368003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44750.050.3780821
GTATCAA78400.041.1835021
ATCAACG96050.033.19843
TCAACGC96900.032.9071854
TATCAAC98000.032.7802732
CAACGCA98700.032.35525
AACGCAG102100.031.2777486
ACGCAGA120850.026.4390747
CGCAGAG121800.026.1151898
GTACATG80150.025.9225921
TACATGG79150.025.2768632
GCAGAGT133550.023.4956029
ACATGGG82300.023.0968173
TGGTATC20800.023.0754412
GAGTACT84500.022.0528612-13
GTACTTT88400.020.76933114-15
GTGGTAT25150.020.7005161
CAGAGTA128700.020.55741510-11
AGAGTAC119550.020.48045210-11
TAAGGTG13150.020.237385
Produced by FastQC (version 0.11.5)