FastQCFastQC Report
Thu 26 May 2016
SRR937957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347679
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101010.4302547324399972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79580.3389730878880801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54060.23026998154347336No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32310.13762528863613807No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32070.13660300236957437No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31150.13268423834774687No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27900.1188407784880301No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46500.049.147151
GTATCAA77100.039.377911
ATCAACG97500.030.9366653
TAAGGTG13550.030.8461935
TCAACGC98250.030.6488974
TATCAAC99750.030.388832
CAACGCA100350.030.0075195
AACGCAG104300.028.9166246
GTAAGGT13150.027.0890454
ACGCAGA122050.024.6333857
CGCAGAG121800.024.5669618
ACCGTCG1401.7816319E-523.748068
TACATGG75400.023.5671162
GTACATG78850.023.439811
GGTAAGG14950.022.8768233
GCAGAGT133950.022.1258549
ACATGGG77950.021.9376853
AGGTAAG15450.021.8341372
AGTATCG1100.002879613521.5891485
GAGTACT85600.021.2789312-13
Produced by FastQC (version 0.11.5)