##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937957_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2347679 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.516755058932674 34.0 31.0 34.0 31.0 34.0 2 32.727619917373715 34.0 31.0 34.0 31.0 34.0 3 32.79271995873371 34.0 31.0 34.0 31.0 34.0 4 36.24475535198807 37.0 35.0 37.0 35.0 37.0 5 36.2067378035924 37.0 37.0 37.0 35.0 37.0 6 36.24376075264122 37.0 37.0 37.0 35.0 37.0 7 36.21045594393441 37.0 37.0 37.0 35.0 37.0 8 36.200639014107125 37.0 37.0 37.0 35.0 37.0 9 37.91838151638277 39.0 38.0 39.0 35.0 39.0 10-11 37.906919770547844 39.0 38.0 39.0 35.0 39.0 12-13 37.85491308649947 39.0 38.0 39.0 35.0 39.0 14-15 39.19284195156152 40.5 39.0 41.0 36.0 41.0 16-17 39.141613482933565 40.0 39.0 41.0 36.0 41.0 18-19 39.16417725762338 40.0 39.0 41.0 36.0 41.0 20-21 39.08304861950889 40.0 39.0 41.0 36.0 41.0 22-23 38.93826945676986 40.0 38.0 41.0 35.0 41.0 24-25 38.89935698193833 40.0 38.0 41.0 35.0 41.0 26-27 38.86765737564633 40.0 38.0 41.0 35.0 41.0 28-29 38.812659439386735 40.0 38.0 41.0 35.0 41.0 30-31 38.62721713658469 40.0 38.0 41.0 35.0 41.0 32-33 38.45764902271563 40.0 38.0 41.0 34.0 41.0 34-35 38.26362846027928 40.0 38.0 41.0 34.0 41.0 36-37 38.13654571174338 40.0 38.0 41.0 34.0 41.0 38-39 38.12440904399622 40.0 38.0 41.0 34.0 41.0 40-41 38.068506171414406 40.0 38.0 41.0 33.5 41.0 42-43 37.91748999756781 40.0 38.0 41.0 33.0 41.0 44-45 37.73124882064371 40.0 37.0 41.0 33.0 41.0 46-47 37.429620276025815 40.0 37.0 41.0 32.5 41.0 48-49 37.10234405981397 40.0 37.0 41.0 31.5 41.0 50-51 36.81365915016491 40.0 36.0 41.0 31.0 41.0 52-53 36.40054049126819 39.0 35.5 41.0 30.0 41.0 54-55 36.181109512842255 39.0 35.0 41.0 30.0 41.0 56-57 35.93019190442986 39.0 35.0 40.5 29.0 41.0 58-59 35.59064165075378 38.5 35.0 40.0 28.5 41.0 60-61 35.231457963375746 38.0 35.0 40.0 28.0 41.0 62-63 34.85859395598802 37.5 34.0 40.0 27.5 41.0 64-65 34.46040365825141 37.0 34.0 40.0 26.5 41.0 66-67 34.03880300501048 36.0 34.0 39.0 26.0 41.0 68-69 33.579253381744266 36.0 33.0 39.0 26.0 40.0 70-71 33.05545753912694 35.0 33.0 38.5 24.5 40.0 72-73 32.64487777076849 35.0 32.0 37.5 24.0 39.5 74-75 32.19333499170883 35.0 32.0 37.0 22.5 39.0 76-77 30.888497959048063 34.0 30.5 35.5 21.5 38.0 78-79 31.60802988824281 35.0 31.5 36.0 23.0 38.0 80-81 31.451531917268078 35.0 32.0 36.0 22.0 37.0 82-83 31.087452969507332 35.0 31.0 35.0 20.0 37.0 84-85 30.71662139500332 34.0 31.0 35.0 18.5 36.5 86-87 30.294005483713917 34.0 31.0 35.0 17.0 36.0 88-89 29.834627519349965 34.0 30.0 35.0 11.0 36.0 90-91 29.49504489327544 34.0 30.0 35.0 6.5 35.0 92-93 29.085952764411147 34.0 29.0 35.0 2.0 35.0 94-95 28.668503658294 34.0 29.0 35.0 2.0 35.0 96-97 28.092556094764234 34.0 29.0 35.0 2.0 35.0 98-99 27.394830170564205 33.5 27.0 35.0 2.0 35.0 100-101 24.91786930836797 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13831931145423226 1101 2 0.13801759698452543 1101 3 0.07775048243680516 1101 4 0.028247907749246792 1101 5 0.08964066973947382 1101 6 0.06021825068908271 1101 7 0.11905579177823 1101 8 0.10870172660192878 1101 9 0.09369432089084029 1101 10-11 0.1066663081670356 1101 12-13 0.12348404302505855 1101 14-15 0.166754726128012 1101 16-17 0.1891309185862653 1101 18-19 0.1917016516152188 1101 20-21 0.15548351711333197 1101 22-23 0.15310867152283691 1101 24-25 0.19771430674023094 1101 26-27 0.08090623558426557 1101 28-29 0.1677540721061206 1101 30-31 0.19137929870779402 1101 32-33 0.21599779982648926 1101 34-35 0.10632046246354321 1101 36-37 0.12569682625473888 1101 38-39 0.31027466335238785 1101 40-41 0.38633872355161003 1101 42-43 0.37611853233801185 1101 44-45 0.40061957084884625 1101 46-47 0.35023182157419086 1101 48-49 0.4297390854772729 1101 50-51 0.33654072875299335 1101 52-53 0.29183792260288755 1101 54-55 0.285902152286873 1101 56-57 0.2922023104867151 1101 58-59 0.3250886833716109 1101 60-61 0.5075263884929058 1101 62-63 0.4257408682357138 1101 64-65 0.34574529618856076 1101 66-67 0.2969972728796506 1101 68-69 0.2664329501814464 1101 70-71 0.3309256172806201 1101 72-73 0.3985368590351257 1101 74-75 0.3728165671028023 1101 76-77 0.310179729029489 1101 78-79 0.35560705982053165 1101 80-81 0.4884113976863027 1101 82-83 0.4198001875280539 1101 84-85 0.5078980373397854 1101 86-87 0.6731146799236782 1101 88-89 0.5220735590731316 1101 90-91 0.5045053026982593 1101 92-93 0.7300417984086778 1101 94-95 0.7466924884314032 1101 96-97 0.8111160350318549 1101 98-99 0.9634282513625827 1101 100-101 1.124692703438523 1102 1 0.13398313408058726 1102 2 0.12250569577859949 1102 3 0.11401503549468828 1102 4 0.08495273422400373 1102 5 0.04092569780360833 1102 6 -0.002986391701647051 1102 7 0.08191640543795842 1102 8 0.11298819260542814 1102 9 0.09530049743089108 1102 10-11 0.10017897436365075 1102 12-13 0.12128871077075587 1102 14-15 0.13224081649590147 1102 16-17 0.2129384318194809 1102 18-19 0.21549483755397603 1102 20-21 0.13588464662512223 1102 22-23 0.16213063396273952 1102 24-25 0.11984046855356212 1102 26-27 -0.02814064766477742 1102 28-29 0.06044708681200461 1102 30-31 0.15810057552155143 1102 32-33 0.08938543650019426 1102 34-35 -0.0112839400969591 1102 36-37 -0.024234077111565 1102 38-39 -0.06010673790910914 1102 40-41 -0.006684253087080094 1102 42-43 -0.05321360734356517 1102 44-45 0.06701741984437604 1102 46-47 0.1613450045953897 1102 48-49 0.17312779886886887 1102 50-51 0.1879399844283398 1102 52-53 0.16164210756218012 1102 54-55 0.06652120863047628 1102 56-57 0.14068520196050116 1102 58-59 0.07896106885745979 1102 60-61 -0.003527336256318847 1102 62-63 0.010858456248719506 1102 64-65 0.16375522630610106 1102 66-67 0.0323555377314122 1102 68-69 0.25411386483592224 1102 70-71 0.2705652684587534 1102 72-73 0.2208608243439656 1102 74-75 0.2422302247481909 1102 76-77 0.13326184700303756 1102 78-79 0.2096666335791788 1102 80-81 0.26951790766156947 1102 82-83 0.3746066909040806 1102 84-85 0.37533659061300995 1102 86-87 0.2515399018664972 1102 88-89 0.05039189071613137 1102 90-91 0.20220221182326625 1102 92-93 0.22577855998860485 1102 94-95 0.16399979500424777 1102 96-97 0.19393157415491658 1102 98-99 0.3796737100444041 1102 100-101 0.5377022558364786 1103 1 0.10038480713151188 1103 2 0.15673195528871275 1103 3 0.10972388685857482 1103 4 0.10769085155295954 1103 5 0.11527150473836656 1103 6 0.13365645550032923 1103 7 0.10406842476304234 1103 8 0.16761045554846277 1103 9 0.20248125419732332 1103 10-11 0.1513735994928851 1103 12-13 0.19057978485022886 1103 14-15 0.22508050067398955 1103 16-17 0.2474265715154189 1103 18-19 0.27623312636319497 1103 20-21 0.2549938439318069 1103 22-23 0.3061012594763568 1103 24-25 0.2833288824522455 1103 26-27 0.3041884146778244 1103 28-29 0.25089963316966646 1103 30-31 0.30886786686237855 1103 32-33 0.36019264049378563 1103 34-35 0.3720988146216442 1103 36-37 0.5017864996917112 1103 38-39 0.37445158857605776 1103 40-41 0.45696348420183597 1103 42-43 0.33555493240672263 1103 44-45 0.2744735391217432 1103 46-47 0.30802845617002816 1103 48-49 0.37347318248761496 1103 50-51 0.36909293653977926 1103 52-53 0.3176876981170196 1103 54-55 0.3936462177429547 1103 56-57 0.3940879875621235 1103 58-59 0.4156966355210301 1103 60-61 0.40984613616102905 1103 62-63 0.4300336775990843 1103 64-65 0.4286230622417335 1103 66-67 0.4864683096146152 1103 68-69 0.4983686546856134 1103 70-71 0.5075428128282411 1103 72-73 0.3898556600542733 1103 74-75 0.38479783933345857 1103 76-77 0.3904282930372851 1103 78-79 0.4189752781726632 1103 80-81 0.3564971295553754 1103 82-83 0.4677734923249659 1103 84-85 0.5598509079724465 1103 86-87 0.6527293701616976 1103 88-89 0.6112174729206252 1103 90-91 0.6439231529599745 1103 92-93 0.763426554912126 1103 94-95 0.7316848984225821 1103 96-97 0.7845135384074986 1103 98-99 0.7215840250379095 1103 100-101 0.9041726659642499 1104 1 0.09589466005461134 1104 2 0.0616987025267548 1104 3 0.04944284298131407 1104 4 0.01035380374272421 1104 5 0.03903732881965283 1104 6 0.014059858616661813 1104 7 0.04510793204860164 1104 8 -0.010381268815500277 1104 9 -0.01882646017033096 1104 10-11 0.016065584689599177 1104 12-13 0.09804219625586086 1104 14-15 0.09492086010425993 1104 16-17 0.06993944245100181 1104 18-19 0.18100663886541213 1104 20-21 0.17061376554541852 1104 22-23 0.21357118177277812 1104 24-25 0.14739131843897013 1104 26-27 0.09740266625169625 1104 28-29 0.15368480803685713 1104 30-31 0.1354140861530695 1104 32-33 0.17010565072224892 1104 34-35 0.23281480735656856 1104 36-37 0.2597698937949744 1104 38-39 0.26215421192537036 1104 40-41 0.28447451155513903 1104 42-43 0.3578834451137851 1104 44-45 0.22716790049118174 1104 46-47 0.15263905062289496 1104 48-49 0.09312576964808983 1104 50-51 0.241082093544712 1104 52-53 0.11937423450386575 1104 54-55 0.1863572396337858 1104 56-57 0.12333198226352948 1104 58-59 0.3047737518852145 1104 60-61 0.34972896626468497 1104 62-63 0.3121277139517957 1104 64-65 0.332776584849519 1104 66-67 0.35490276685033706 1104 68-69 0.29600135846973785 1104 70-71 0.423724685634717 1104 72-73 0.31873093829863564 1104 74-75 0.28153265026385554 1104 76-77 0.3244922312021714 1104 78-79 0.3930763611065551 1104 80-81 0.3421588390341199 1104 82-83 0.4424590591240971 1104 84-85 0.371295457114158 1104 86-87 0.34540966568111386 1104 88-89 0.2421138635961917 1104 90-91 0.3112473639750384 1104 92-93 0.5001869028712136 1104 94-95 0.42963852208598396 1104 96-97 0.5159056478753179 1104 98-99 0.6532130689697944 1104 100-101 0.7519125706442189 1105 1 0.08361463238996691 1105 2 0.07824625753585224 1105 3 0.07317295901912502 1105 4 0.07131591855099373 1105 5 0.06724758010682308 1105 6 0.060971920913338806 1105 7 0.012281783837842397 1105 8 -0.017589445254451164 1105 9 0.011128200553564227 1105 10-11 0.0017119895667718765 1105 12-13 0.10676424343944291 1105 14-15 0.08374997970260978 1105 16-17 0.05982007463932604 1105 18-19 0.02842972859619408 1105 20-21 0.08345220915309426 1105 22-23 0.11047299790536158 1105 24-25 0.03674811548808066 1105 26-27 0.055011723860758366 1105 28-29 0.05553936373756585 1105 30-31 0.09304619763133815 1105 32-33 0.06745189422233722 1105 34-35 0.15996970374223451 1105 36-37 0.22367265769774036 1105 38-39 0.23409637232906988 1105 40-41 0.23160942792082295 1105 42-43 0.2514680656214807 1105 44-45 0.3730886464119294 1105 46-47 0.38550618349002974 1105 48-49 0.4769534803988762 1105 50-51 0.4921566600038858 1105 52-53 0.5420297840824944 1105 54-55 0.588584499553356 1105 56-57 0.5210509299824793 1105 58-59 0.5674175183751302 1105 60-61 0.6228058893416062 1105 62-63 0.5253708810411197 1105 64-65 0.5900603267486488 1105 66-67 0.4531519535610542 1105 68-69 0.46870685809830803 1105 70-71 0.5197504547374052 1105 72-73 0.42821120162505366 1105 74-75 0.33910584629858675 1105 76-77 0.5501409414662639 1105 78-79 0.5111185753026142 1105 80-81 0.5698708705923003 1105 82-83 0.6810512361778152 1105 84-85 0.7296985924403678 1105 86-87 0.742069416187018 1105 88-89 0.608105564203278 1105 90-91 0.5435394308824897 1105 92-93 0.6832602397907017 1105 94-95 0.5943622598866476 1105 96-97 0.6456336122186634 1105 98-99 0.7700372704093787 1105 100-101 0.6845017763103485 1106 1 -0.17545751717991465 1106 2 -0.09630995548189958 1106 3 -0.06266563677421999 1106 4 -0.04571953468355616 1106 5 -0.15164257950731752 1106 6 -0.10558618161761046 1106 7 -0.0718388466628781 1106 8 -0.13215361411725723 1106 9 -0.2372756580312938 1106 10-11 -0.1217813722750094 1106 12-13 -0.2120227388090754 1106 14-15 -0.41150261837804436 1106 16-17 -0.22997779717211841 1106 18-19 -0.2068594166477027 1106 20-21 -0.1432645130387371 1106 22-23 -0.11070943956666923 1106 24-25 0.04078322065440432 1106 26-27 -0.13773229682443855 1106 28-29 -0.15346441528883759 1106 30-31 -0.20820063246164722 1106 32-33 -0.13088110761744787 1106 34-35 -0.23606729093729228 1106 36-37 -0.2252122808930821 1106 38-39 -0.2792769059763387 1106 40-41 -0.2553777930030492 1106 42-43 -0.24247306112507516 1106 44-45 -0.17952932385310305 1106 46-47 -0.24632962775755374 1106 48-49 -0.29319966961852373 1106 50-51 -0.3268578096893151 1106 52-53 -0.3668111487403394 1106 54-55 -0.6120830455712039 1106 56-57 -0.39289541778739334 1106 58-59 -0.3425397189576671 1106 60-61 -0.3000609497017024 1106 62-63 -0.2291888126588475 1106 64-65 -0.35076683251742935 1106 66-67 -0.4800985063862342 1106 68-69 -0.6190610301220616 1106 70-71 -0.6896697630538426 1106 72-73 -0.5149716336392274 1106 74-75 -0.5040670441593704 1106 76-77 -0.441639676106206 1106 78-79 -0.4346610975132563 1106 80-81 -0.6331212730106976 1106 82-83 -0.6766012922891953 1106 84-85 -0.6031888820760649 1106 86-87 -0.394665455276364 1106 88-89 -0.26206871852756564 1106 90-91 -0.5507783554036223 1106 92-93 -0.5609730156416468 1106 94-95 -0.6377793541554198 1106 96-97 -0.4471004636602096 1106 98-99 -0.4292417311320662 1106 100-101 -0.342727103407217 1107 1 0.09757594470183051 1107 2 0.03197518623009188 1107 3 0.027362318295580224 1107 4 0.04090581685986905 1107 5 -0.0018808712167839303 1107 6 0.004611524719429383 1107 7 -0.005574384966379853 1107 8 0.014260221958252828 1107 9 0.015681516822617425 1107 10-11 0.10331714408545167 1107 12-13 0.03227299551672047 1107 14-15 0.12294400596307042 1107 16-17 0.00965567144578472 1107 18-19 -0.007655967733072089 1107 20-21 -0.010466143189795218 1107 22-23 0.0034895466410418408 1107 24-25 -0.03056199077686017 1107 26-27 -0.08062438351883827 1107 28-29 6.19803569051669E-4 1107 30-31 -0.00813621791827046 1107 32-33 -0.04393368335716019 1107 34-35 0.11030528614965363 1107 36-37 0.08388869416628353 1107 38-39 0.06732385857798562 1107 40-41 0.12570285568929052 1107 42-43 0.13713068418066143 1107 44-45 0.08052439703404701 1107 46-47 0.14484684283068106 1107 48-49 0.10020226029171653 1107 50-51 0.11447780271195995 1107 52-53 0.01807206306141751 1107 54-55 0.24683100100935462 1107 56-57 0.042427749555692174 1107 58-59 0.1273925826202813 1107 60-61 0.12161559666834876 1107 62-63 0.18973053864880995 1107 64-65 0.18414121887948198 1107 66-67 0.025996035638989667 1107 68-69 0.24298406847849918 1107 70-71 0.0748264562755736 1107 72-73 0.016688324933376464 1107 74-75 0.018895688027690483 1107 76-77 -4.884661232154031E-4 1107 78-79 0.10169186050361745 1107 80-81 0.29623365533338486 1107 82-83 0.2797205951832282 1107 84-85 0.3423635673606249 1107 86-87 0.2274161027977719 1107 88-89 0.3203183244659833 1107 90-91 0.3339217170101101 1107 92-93 0.5117602660477836 1107 94-95 0.564728181925144 1107 96-97 0.6868097938295641 1107 98-99 0.7364661552288929 1107 100-101 0.5953860977850809 1108 1 0.05798881589876714 1108 2 -0.012300215222161626 1108 3 -0.021417546629074025 1108 4 0.017415492054411175 1108 5 -0.0576347491024265 1108 6 0.05981202311883038 1108 7 0.02670966365033678 1108 8 0.081583016898783 1108 9 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96-97 -0.25164190986832224 2316 98-99 -0.19892219591056914 2316 100-101 -0.06521906315407833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 17.0 5 37.0 6 79.0 7 185.0 8 484.0 9 896.0 10 1491.0 11 2416.0 12 3772.0 13 5483.0 14 7357.0 15 10160.0 16 12931.0 17 18008.0 18 18137.0 19 13532.0 20 11864.0 21 11544.0 22 12100.0 23 12878.0 24 14734.0 25 17469.0 26 20439.0 27 25242.0 28 30926.0 29 37659.0 30 46750.0 31 57563.0 32 72182.0 33 94814.0 34 133825.0 35 203906.0 36 342481.0 37 569104.0 38 484368.0 39 52828.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80752223792094 18.466025380812283 13.85108441145489 23.875367969811887 2 16.248782644925964 19.726818279028432 39.15644429221293 24.867954783832673 3 19.88283790916104 22.898407125486205 29.13563565247877 28.083119312873983 4 13.264973618625033 15.906433545642312 35.56380578435127 35.26478705138138 5 13.929076334541476 37.01562266391615 34.00332839370289 15.051972607839486 6 33.9815622152773 35.17444250257382 16.31888345893966 14.525111823209222 7 29.97437043139203 32.09101414631216 19.43234147428162 18.50227394801419 8 27.194033894146603 34.621678929931136 18.949363238830802 19.234923937091455 9 26.471755850050606 16.350410108396744 19.683371442007232 37.49446259954542 10-11 25.531045769034012 26.139859836033803 26.406719146868035 21.922375248064153 12-13 26.30091251827869 23.270238392897838 27.186787461147798 23.242061627675675 14-15 22.865796388688572 24.878805833335818 25.357832139743124 26.897565638232486 16-17 22.58297416570064 28.158728204752492 26.39574881260107 22.862548816945797 18-19 22.69224199730883 26.455256446899256 28.564552479278472 22.287949076513442 20-21 22.80401196245313 25.572874315440906 29.29176007452467 22.33135364758129 22-23 22.693690236186466 25.628056476204797 28.998023153932035 22.68023013367671 24-25 22.641121633471286 25.889223210419587 28.981736417326985 22.48791873878214 26-27 22.093895161451808 26.26796258133728 28.9011111674437 22.737031089767214 28-29 22.287314894266828 26.681293882446 28.74290495909044 22.288486264196738 30-31 22.27719377308397 26.198087557966826 28.71355070263013 22.811167966319076 32-33 22.059703940187443 26.097605389178835 28.823566369598762 23.019124301034964 34-35 22.86228877871092 26.252054298449757 28.581391757243964 22.30426516559536 36-37 22.936118443724364 25.76102870233547 28.545386545032137 22.75746630890803 38-39 22.384781487585563 26.139436143583556 28.64275226313044 22.833030105700445 40-41 22.80839762510776 26.055097619335182 28.56810561381737 22.568399141739693 42-43 22.432585500965185 25.98186944389362 28.49414487533086 23.091400179810336 44-45 22.4578118540634 26.349240088370784 28.723486049739556 22.469462007826262 46-47 22.831762775064224 26.57499172587053 27.954950399948203 22.63829509911704 48-49 22.63778395598376 26.56231963569125 27.957271841678526 22.842624566646464 50-51 22.859385801891996 26.854480531622936 27.83223345269945 22.453900213785616 52-53 23.021673746708984 26.520427196392692 27.85042162919207 22.607477427706257 54-55 22.5403047009408 26.711360454304018 28.039182528786945 22.70915231596824 56-57 22.709386589904327 26.861104094725047 27.788339036128875 22.641170279241756 58-59 22.61154527514196 26.451934016532924 27.83057223751629 23.10594847080883 60-61 22.355398672476092 26.79780753672031 27.960807248350395 22.885986542453207 62-63 22.37624905278788 26.83603678356368 28.17186676713469 22.61584739651375 64-65 22.091861792008192 26.78543361336878 28.051599047399577 23.07110554722345 66-67 22.095141627113417 26.812822366260463 27.9230891446403 23.168946861985816 68-69 22.493277916745203 26.55973096758598 28.04509572778997 22.901895387878845 70-71 22.36794297687205 26.66031003386749 27.745104846105452 23.226642143155004 72-73 22.349669609857227 26.787094828551943 27.72335996531042 23.13987559628041 74-75 22.340682009763686 27.20148282622965 27.38027643472553 23.077558729281133 76-77 22.45330388013012 26.80898879276085 27.681893478622932 23.0558138484861 78-79 22.47641180928057 26.87656617450682 27.71013413673675 22.936887879475858 80-81 22.556001054658665 27.042197847320693 27.464465968303166 22.93733512971748 82-83 22.432695141919073 27.14774100003578 27.449912168459363 22.969651689585785 84-85 22.489810922758956 27.19667900828587 27.371218071810844 22.94229199714433 86-87 22.531280489012616 27.186365031364083 27.24327253048183 23.03908194914148 88-89 22.395329510831345 27.336426603830084 27.08700983993965 23.18123404539892 90-91 22.463028252255107 27.242083750235874 27.092488683465444 23.20239931404358 92-93 22.46256664845714 26.90138850286827 27.344976780194003 23.29106806848059 94-95 22.242244361345822 26.833992211030555 27.541116140664883 23.382647286958736 96-97 22.483972468127032 26.489460441568035 27.381554292558736 23.645012797746197 98-99 22.170982489514113 26.832906031872326 27.17158095293266 23.824530525680895 100-101 22.279643000597613 26.52343016230072 27.44557497000229 23.751351867099377 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1337.0 1 857.0 2 597.0 3 840.5 4 660.5 5 585.0 6 1198.0 7 2464.5 8 3802.5 9 4196.0 10 3360.0 11 2536.0 12 2408.0 13 2431.5 14 2554.0 15 2682.0 16 2711.0 17 2771.5 18 2975.5 19 3411.0 20 3868.0 21 4449.5 22 5322.5 23 6538.0 24 8092.5 25 10165.0 26 12884.0 27 15934.0 28 18543.0 29 21237.5 30 24432.5 31 28515.0 32 35979.5 33 38948.0 34 38635.0 35 41716.0 36 46325.0 37 51196.0 38 54224.0 39 57108.5 40 60786.5 41 65733.5 42 68549.0 43 70876.0 44 74469.0 45 79333.0 46 83669.5 47 88067.5 48 90114.0 49 89055.0 50 89642.5 51 87602.5 52 86984.5 53 90438.0 54 92764.5 55 87050.0 56 77816.5 57 70799.5 58 64958.0 59 57504.0 60 46209.5 61 37399.0 62 31346.0 63 23516.0 64 16694.5 65 12647.5 66 9379.0 67 7215.5 68 5385.0 69 3815.5 70 2800.0 71 1967.5 72 1421.5 73 1175.0 74 914.5 75 655.0 76 456.0 77 267.0 78 149.5 79 84.0 80 51.0 81 33.5 82 25.5 83 14.5 84 9.5 85 6.5 86 1.5 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01605841343727145 3 0.006900432299304974 4 0.0 5 0.0 6 0.0 7 0.0 8 7.241194388159539E-4 9 2.981668277477457E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3427393608751453E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005899443663294684 26-27 0.021638392642264977 28-29 2.129763055341041E-5 30-31 0.0 32-33 4.898455027284395E-4 34-35 0.019870689306331915 36-37 0.004259526110682083 38-39 0.018422450428700006 40-41 0.015866734762290755 42-43 0.008753326157451679 44-45 0.003002965908030868 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.7686919719433536E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.2778578332046247E-4 84-85 0.002853882494156995 86-87 4.046549805147978E-4 88-89 8.519052221364164E-5 90-91 3.4076208885456654E-4 92-93 2.7686919719433536E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2347679.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.05010622128388 #Duplication Level Percentage of deduplicated Percentage of total 1 75.14273669926025 38.36044690255203 2 13.866275274500312 14.157496513136067 3 4.543505675198305 6.958393420076389 4 2.073514246525968 4.234124901459844 5 1.1166545544303843 2.8502666808075774 6 0.700613839809018 2.1459846565411165 7 0.44068674787152506 1.574797370240745 8 0.31973701757136536 1.305808696791577 9 0.23588239611295594 1.0837639239567627 >10 1.379579230725721 13.127378810014575 >50 0.11711582140739073 4.117929636639599 >100 0.057024674506973516 5.421393734888666 >500 0.00358717936792605 1.2678699545869314 >1k 0.002836374383941243 2.3950310875030913 >5k 1.6684555199654374E-4 0.5691382242147891 >10k+ 8.342277599827187E-5 0.4301754865903611 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10101 0.4302547324399972 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7958 0.3389730878880801 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5406 0.23026998154347336 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3231 0.13762528863613807 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3207 0.13660300236957437 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3115 0.13268423834774687 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2790 0.1188407784880301 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.1297630553410412E-5 0.0 20-21 0.0 0.0 0.0 8.519052221364165E-5 0.0 22-23 0.0 0.0 0.0 2.3427393608751453E-4 0.0 24-25 0.0 0.0 0.0 5.111431332818499E-4 0.0 26-27 0.0 0.0 0.0 0.0011713696804375726 0.0 28-29 0.0 0.0 0.0 0.0040678474357013885 0.0 30-31 0.0 0.0 0.0 0.013736971706949715 0.0 32-33 0.0 0.0 0.0 0.03505589989091354 0.0 34-35 0.0 0.0 0.0 0.06781165568205876 0.0 36-37 0.0 0.0 0.0 0.12207801833214847 0.0 38-39 0.0 0.0 0.0 0.20358405046005013 0.0 40-41 0.0 0.0 0.0 0.2903931925957509 0.0 42-43 0.0 0.0 0.0 0.37586058400658695 0.0 44-45 0.0 0.0 0.0 0.4785151632740251 0.0 46-47 0.0 0.0 0.0 0.5980587635703177 0.0 48-49 0.0 0.0 0.0 0.7214998302578846 0.0 50-51 0.0 0.0 0.0 0.8584648923468668 0.0 52-53 0.0 0.0 0.0 0.9863358661895429 0.0 54-55 0.0 0.0 0.0 1.1226194041008162 0.0 56-57 0.0 0.0 0.0 1.2785393573823338 0.0 58-59 0.0 0.0 0.0 1.4448525543739157 0.0 60-61 0.0 0.0 0.0 1.6224747931893586 0.0 62-63 0.0 0.0 0.0 1.7936864452082246 0.0 64-65 0.0 0.0 0.0 1.968795563618365 0.0 66-67 0.0 0.0 0.0 2.166522765676228 2.1297630553410412E-5 68-69 0.0 0.0 0.0 2.3874430874067536 4.2595261106820824E-5 70-71 0.0 0.0 0.0 2.6093856954038435 4.2595261106820824E-5 72-73 0.0 0.0 0.0 2.836950877867034 4.2595261106820824E-5 74-75 4.2595261106820824E-5 0.0 0.0 3.0663050612967107 4.2595261106820824E-5 76-77 4.2595261106820824E-5 0.0 0.0 3.3093749188027832 4.2595261106820824E-5 78-79 4.2595261106820824E-5 0.0 0.0 3.5802168865505037 4.2595261106820824E-5 80-81 4.2595261106820824E-5 0.0 0.0 3.868033065849292 4.2595261106820824E-5 82-83 4.2595261106820824E-5 0.0 0.0 4.158617937120024 4.2595261106820824E-5 84-85 4.2595261106820824E-5 0.0 0.0 4.451140892771116 4.2595261106820824E-5 86-87 4.2595261106820824E-5 0.0 0.0 4.755654414423778 6.389289166023124E-5 88-89 4.2595261106820824E-5 0.0 0.0 5.0783135173079454 8.519052221364165E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4650 0.0 49.14715 1 GTATCAA 7710 0.0 39.37791 1 ATCAACG 9750 0.0 30.936665 3 TAAGGTG 1355 0.0 30.846193 5 TCAACGC 9825 0.0 30.648897 4 TATCAAC 9975 0.0 30.38883 2 CAACGCA 10035 0.0 30.007519 5 AACGCAG 10430 0.0 28.916624 6 GTAAGGT 1315 0.0 27.089045 4 ACGCAGA 12205 0.0 24.633385 7 CGCAGAG 12180 0.0 24.566961 8 ACCGTCG 140 1.7816319E-5 23.74806 8 TACATGG 7540 0.0 23.567116 2 GTACATG 7885 0.0 23.43981 1 GGTAAGG 1495 0.0 22.876823 3 GCAGAGT 13395 0.0 22.125854 9 ACATGGG 7795 0.0 21.937685 3 AGGTAAG 1545 0.0 21.834137 2 AGTATCG 110 0.0028796135 21.589148 5 GAGTACT 8560 0.0 21.27893 12-13 >>END_MODULE