FastQCFastQC Report
Thu 26 May 2016
SRR937956_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937956_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453179
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99620.40608532846563583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79640.3246399875426946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51040.2080565666019479No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34450.14043002976953578No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33830.1379026968680231No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29220.11911075384225937No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27620.11258860441900082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48650.048.734541
GTATCAA83950.040.2083171
ATCAACG102650.032.7155533
TCAACGC103300.032.371554
TATCAAC105350.031.8770922
CAACGCA105350.031.831945
AACGCAG108800.030.8587136
CGCAGAG126100.026.5431888
ACGCAGA126600.026.4780777
TACATGG82150.025.5352152
GTACATG84400.025.3276141
GCAGAGT137400.023.73699
ACATGGG85600.023.6725123
TAAGGTG13200.023.4232925
GAGTACT85650.022.36717212-13
GTAAGGT12600.022.2735794
GTACTTT89550.021.25991214-15
GAGTACA62950.020.9495371
CAGAGTA130800.020.73539710-11
AGAGTAC122350.020.71036310-11
Produced by FastQC (version 0.11.5)