##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937956_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2453179 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95958876217349 33.0 31.0 34.0 30.0 34.0 2 32.06921468021697 33.0 31.0 34.0 30.0 34.0 3 32.10108557100807 34.0 31.0 34.0 30.0 34.0 4 35.61311180309305 37.0 35.0 37.0 33.0 37.0 5 35.61867234311072 37.0 35.0 37.0 33.0 37.0 6 35.64980378521094 37.0 35.0 37.0 33.0 37.0 7 35.579316470587756 37.0 35.0 37.0 33.0 37.0 8 35.56462695954922 37.0 35.0 37.0 33.0 37.0 9 37.2054346625338 39.0 37.0 39.0 34.0 39.0 10-11 37.11098089458616 39.0 37.0 39.0 34.0 39.0 12-13 36.95403678247695 39.0 37.0 39.0 33.0 39.0 14-15 38.14370190679115 40.0 38.0 41.0 33.0 41.0 16-17 37.9547735815446 40.0 38.0 41.0 33.0 41.0 18-19 38.129943024948446 40.0 38.0 41.0 33.0 41.0 20-21 38.1220993249983 40.0 38.0 41.0 33.0 41.0 22-23 38.00671068030502 40.0 38.0 41.0 33.0 41.0 24-25 37.882087691114265 40.0 38.0 41.0 33.0 41.0 26-27 37.69253425860893 40.0 38.0 41.0 32.0 41.0 28-29 37.513930292082236 40.0 37.0 41.0 32.0 41.0 30-31 37.2865190432496 40.0 37.0 41.0 31.5 41.0 32-33 36.99142337350841 40.0 36.5 41.0 31.0 41.0 34-35 36.752712296982814 39.0 36.0 41.0 30.0 41.0 36-37 36.4836208446265 39.0 35.5 41.0 30.0 41.0 38-39 36.17356540228006 39.0 35.0 40.5 29.5 41.0 40-41 36.05793849531567 39.0 35.0 40.0 29.5 41.0 42-43 35.82883352580468 39.0 35.0 40.5 27.5 41.0 44-45 35.505084423109764 39.0 35.0 40.0 27.0 41.0 46-47 35.13211306635186 38.0 34.5 40.0 25.5 41.0 48-49 34.72771473259799 38.0 34.0 40.0 24.0 41.0 50-51 33.98317876518591 37.5 33.5 39.5 23.0 40.5 52-53 34.04442480552785 38.0 33.5 39.5 23.0 40.5 54-55 34.116015382489415 38.0 34.0 40.0 22.5 41.0 56-57 33.78152531878024 38.0 33.0 40.0 21.0 41.0 58-59 33.350089822226586 37.0 33.0 40.0 19.0 41.0 60-61 32.820024343922725 37.0 32.0 40.0 15.0 41.0 62-63 32.254787155768085 36.0 31.0 39.0 10.0 41.0 64-65 31.685852520341975 35.5 31.0 39.0 7.5 40.0 66-67 31.093791565964 35.0 30.0 38.5 3.5 40.0 68-69 30.389019309231003 35.0 29.0 38.0 2.0 40.0 70-71 29.756285008146573 34.0 28.5 37.0 2.0 39.5 72-73 29.041507366563955 34.0 27.5 36.5 2.0 39.0 74-75 28.344679699279993 34.0 26.0 36.0 2.0 39.0 76-77 27.570891891704598 33.0 25.5 35.0 2.0 37.5 78-79 26.77466992828489 32.5 24.5 35.0 2.0 37.0 80-81 26.034982159883157 32.0 21.5 35.0 2.0 36.5 82-83 25.263940992483633 31.5 19.0 35.0 2.0 36.0 84-85 24.51393131116808 31.0 14.5 34.0 2.0 35.5 86-87 23.735490765247867 31.0 7.0 34.0 2.0 35.0 88-89 22.81589174699441 30.0 2.0 34.0 2.0 35.0 90-91 21.875873713251252 29.0 2.0 34.0 2.0 35.0 92-93 20.893440103636955 29.0 2.0 34.0 2.0 35.0 94-95 19.776264186184537 26.5 2.0 33.5 2.0 35.0 96-97 18.46655788264941 25.0 2.0 33.0 2.0 35.0 98-99 16.867136275012953 11.0 2.0 32.0 2.0 34.0 100-101 13.013811466672427 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13764767143230117 1101 2 0.07140488125862987 1101 3 0.008441628589238803 1101 4 -0.07248332247854705 1101 5 0.04792812385015566 1101 6 0.052430299204154096 1101 7 0.07930793864710495 1101 8 0.030609687865911894 1101 9 0.06643328990534769 1101 10-11 0.13341861598720328 1101 12-13 0.14262705417856836 1101 14-15 0.171360447318186 1101 16-17 0.25425293335549526 1101 18-19 0.3252874763624902 1101 20-21 0.3707675996819333 1101 22-23 0.29813435341235817 1101 24-25 0.4135543586905399 1101 26-27 0.2904492153777056 1101 28-29 0.11880924105216906 1101 30-31 0.11675402531139412 1101 32-33 0.18065120463621298 1101 34-35 0.2571040614546831 1101 36-37 0.13006433968035225 1101 38-39 0.17309310789803334 1101 40-41 0.26690926825165917 1101 42-43 0.32094874619499336 1101 44-45 0.33558320313638035 1101 46-47 0.2854025730640686 1101 48-49 0.2975114792886231 1101 50-51 0.37072729210628097 1101 52-53 0.333983437393627 1101 54-55 0.2602712388393087 1101 56-57 0.166070576261518 1101 58-59 0.09995358754734696 1101 60-61 0.2412140801217646 1101 62-63 -0.033485449973333914 1101 64-65 0.09839605547259467 1101 66-67 -0.020735771389372104 1101 68-69 0.12648502799871508 1101 70-71 0.14931750808787925 1101 72-73 0.09353288703418272 1101 74-75 -0.04435867179392261 1101 76-77 -0.028515492811269638 1101 78-79 -0.031099784674331943 1101 80-81 0.09564762496687962 1101 82-83 0.032058192823658516 1101 84-85 -0.08781981187153676 1101 86-87 0.17691596868561987 1101 88-89 0.2868419613927422 1101 90-91 0.17812182871402626 1101 92-93 0.07104492120786787 1101 94-95 0.0024020217112799003 1101 96-97 0.012751495717502337 1101 98-99 -0.2672788312143872 1101 100-101 -0.46958223685030553 1102 1 0.20666766192972474 1102 2 0.2307772006856652 1102 3 0.28658027061296565 1102 4 0.1789804895871825 1102 5 0.1546210661108134 1102 6 0.2275979496112015 1102 7 0.2628907944597785 1102 8 0.2804134181074005 1102 9 0.3169245223391286 1102 10-11 0.33579872561765 1102 12-13 0.29876785819899254 1102 14-15 0.35477652963987794 1102 16-17 0.27834484691389605 1102 18-19 0.2821377851963831 1102 20-21 0.3068703933770678 1102 22-23 0.30270962310390814 1102 24-25 0.2935625857575914 1102 26-27 0.33644202785478683 1102 28-29 0.24070312464235855 1102 30-31 0.3403191514756969 1102 32-33 0.16049425260511185 1102 34-35 0.260948142590145 1102 36-37 0.3261188551603169 1102 38-39 0.12213950154087883 1102 40-41 0.17105341411947705 1102 42-43 0.28511955031107306 1102 44-45 0.11417401496932911 1102 46-47 0.059573727946578 1102 48-49 -0.02438686860151762 1102 50-51 -0.05044369377058899 1102 52-53 0.0399981249404604 1102 54-55 -0.08764760018760853 1102 56-57 0.003836640566575511 1102 58-59 -0.1315764874847858 1102 60-61 -0.08960967888257443 1102 62-63 -0.09565082677618619 1102 64-65 -0.3181559743489295 1102 66-67 -0.2705484302307575 1102 68-69 -0.40956132418990876 1102 70-71 -0.5031128249170074 1102 72-73 -0.4438119386827388 1102 74-75 -0.5139146971639015 1102 76-77 -0.611108549549332 1102 78-79 -0.5729777881118459 1102 80-81 -0.5147656735449146 1102 82-83 -0.7723270252414522 1102 84-85 -0.6213706203881451 1102 86-87 -0.7034612008844405 1102 88-89 -0.6829356845818957 1102 90-91 -0.6184610685528611 1102 92-93 -1.0193485430570028 1102 94-95 -0.8786757240723482 1102 96-97 -1.0626396885656142 1102 98-99 -1.2968771398824632 1102 100-101 -1.4203311231669087 1103 1 0.26816870319557395 1103 2 0.25240607468218457 1103 3 0.33908924184554934 1103 4 0.22242901156422334 1103 5 0.23483252379804043 1103 6 0.11503705063078229 1103 7 0.23255489872636304 1103 8 0.19863813974529876 1103 9 0.24298675714204876 1103 10-11 0.2754192654412648 1103 12-13 0.23505005560778613 1103 14-15 0.31823572524936594 1103 16-17 0.3261885546699972 1103 18-19 0.2851285669489201 1103 20-21 0.37013634687676955 1103 22-23 0.3188721042743268 1103 24-25 0.19460126209769868 1103 26-27 0.2048466192160987 1103 28-29 0.12925500646687027 1103 30-31 0.2646885959167804 1103 32-33 0.1768038097950253 1103 34-35 0.01855017227967437 1103 36-37 0.1952150275018525 1103 38-39 0.19059165489969843 1103 40-41 0.26215294796739386 1103 42-43 0.312292805862036 1103 44-45 0.39102641416965866 1103 46-47 0.453141873101508 1103 48-49 0.5079464894130723 1103 50-51 0.7216086054099549 1103 52-53 0.602645934316989 1103 54-55 0.5480145514273431 1103 56-57 0.46152760370612356 1103 58-59 0.5465092058420922 1103 60-61 0.5497662939534749 1103 62-63 0.5269365297033133 1103 64-65 0.541544832161744 1103 66-67 0.32594911722098274 1103 68-69 0.35270404707487657 1103 70-71 0.2326648026153002 1103 72-73 0.16955241615930206 1103 74-75 0.27563944603028645 1103 76-77 0.22876511477990036 1103 78-79 -0.02473048110006104 1103 80-81 0.07083651475204533 1103 82-83 0.020986862497668568 1103 84-85 -0.0323571585827942 1103 86-87 -0.30391179568252014 1103 88-89 -0.3693178208539969 1103 90-91 0.07331938478115063 1103 92-93 -0.16420244012644858 1103 94-95 -0.5316456076779659 1103 96-97 -0.8926969895446497 1103 98-99 -0.7421114520097412 1103 100-101 -0.8838611256633566 1104 1 0.3024129189561755 1104 2 0.31412087110105347 1104 3 0.29763774537155996 1104 4 0.2988259982560635 1104 5 0.2845841030348737 1104 6 0.22914647362147633 1104 7 0.27568431663267745 1104 8 0.34775877972764846 1104 9 0.4652799944991841 1104 10-11 0.42800491517120065 1104 12-13 0.42137176625661965 1104 14-15 0.3858596985990488 1104 16-17 0.46436049288834624 1104 18-19 0.4735246372433295 1104 20-21 0.4254023646615295 1104 22-23 0.4478332283985864 1104 24-25 0.4208007096150581 1104 26-27 0.3329309141812402 1104 28-29 0.3405340041562397 1104 30-31 0.24635507560210357 1104 32-33 0.396756852925769 1104 34-35 0.2614475255682649 1104 36-37 0.3039264503240773 1104 38-39 0.17856337198899297 1104 40-41 0.26487626506924045 1104 42-43 0.2735085320881083 1104 44-45 0.2865351035135717 1104 46-47 0.17837562810608887 1104 48-49 0.1404561038424461 1104 50-51 -0.04303127797412998 1104 52-53 0.2578269416968979 1104 54-55 0.11597981293868997 1104 56-57 0.30823284141229124 1104 58-59 0.2813421861773193 1104 60-61 0.23659077664787986 1104 62-63 0.221454161288122 1104 64-65 0.1796033539212729 1104 66-67 0.36003628754360406 1104 68-69 0.251395196587044 1104 70-71 0.038977443724828476 1104 72-73 -0.16027218401226762 1104 74-75 -0.1606867030644814 1104 76-77 -0.18546012331911754 1104 78-79 -0.23009444688738512 1104 80-81 -0.37730024453271227 1104 82-83 -0.2527742161466868 1104 84-85 -0.34562182254710905 1104 86-87 -0.3124775241561508 1104 88-89 -0.1627940985881473 1104 90-91 -0.2641309376766685 1104 92-93 -0.44347814618876313 1104 94-95 -0.6305225675351487 1104 96-97 -0.9989192941142093 1104 98-99 -1.0937427036552698 1104 100-101 -1.3804346454038647 1105 1 0.1286439569423088 1105 2 0.16335740089194672 1105 3 0.2479041610692292 1105 4 0.08532611662764822 1105 5 0.147212546825358 1105 6 0.10558935959512894 1105 7 0.1918639611781856 1105 8 0.20614230155314317 1105 9 0.2641284459546185 1105 10-11 0.09677334106262236 1105 12-13 0.2424363886512495 1105 14-15 0.22146013981189583 1105 16-17 0.2662157419517257 1105 18-19 0.27780013411205573 1105 20-21 0.29100575720188715 1105 22-23 0.27950697081988807 1105 24-25 0.3281207980699534 1105 26-27 0.3010610889571552 1105 28-29 0.19703180817747779 1105 30-31 0.1303973645158294 1105 32-33 0.10272713369469244 1105 34-35 0.16784585498949411 1105 36-37 0.1578837771007926 1105 38-39 0.08688961895141034 1105 40-41 0.3559332118890737 1105 42-43 0.49281366431926443 1105 44-45 0.38544863280230857 1105 46-47 0.5384779266381727 1105 48-49 0.4553380666663358 1105 50-51 0.4353149488385739 1105 52-53 0.406192192894153 1105 54-55 0.4009408352445618 1105 56-57 0.29089458849201577 1105 58-59 0.5550695854314185 1105 60-61 0.5245631084832567 1105 62-63 0.46553797049647727 1105 64-65 0.46069659782301287 1105 66-67 0.5033444708953603 1105 68-69 0.31074262410169595 1105 70-71 0.21747171464607717 1105 72-73 0.3340109459661029 1105 74-75 0.09925199850004063 1105 76-77 -0.052998864800873235 1105 78-79 -0.27911116647623757 1105 80-81 -0.22657711621047838 1105 82-83 -0.29642944588842113 1105 84-85 -0.2986389179524842 1105 86-87 -0.3129281854976895 1105 88-89 -0.21815457988808973 1105 90-91 -0.5564700133591103 1105 92-93 -0.7567750975099052 1105 94-95 -0.7991930581880418 1105 96-97 -1.0258748405397604 1105 98-99 -0.8790070863341626 1105 100-101 -0.9403540253278297 1106 1 0.21575614832126533 1106 2 0.25387688080380144 1106 3 0.30892772785582423 1106 4 0.31972487582721243 1106 5 0.38783090399684284 1106 6 0.21948925650701057 1106 7 0.3151314647145327 1106 8 0.205517300578407 1106 9 0.46263546171727654 1106 10-11 0.3684419260105116 1106 12-13 0.36636632303979155 1106 14-15 0.4330424644880111 1106 16-17 0.09361702454886256 1106 18-19 0.4668276435124099 1106 20-21 0.5814475701180015 1106 22-23 0.4884834352248575 1106 24-25 0.5201380189327409 1106 26-27 0.31093360903282985 1106 28-29 0.3632190994864146 1106 30-31 0.29410492644278463 1106 32-33 0.2520694205666274 1106 34-35 0.1968604432842369 1106 36-37 0.07942899941087234 1106 38-39 0.10816616492231645 1106 40-41 0.08740503264986899 1106 42-43 0.051184973382440546 1106 44-45 0.1221358781036912 1106 46-47 0.3350884562865346 1106 48-49 0.3194783335725546 1106 50-51 0.3474428529681859 1106 52-53 0.19280855536508312 1106 54-55 0.07896474045963942 1106 56-57 -0.0379717229027321 1106 58-59 0.09689201497357658 1106 60-61 0.31908945677762546 1106 62-63 0.17601338452550408 1106 64-65 -0.017858421757491527 1106 66-67 -0.14836525572617276 1106 68-69 -0.06371054878222893 1106 70-71 -0.3072604667827754 1106 72-73 -0.3225751918787765 1106 74-75 -0.4159573982706952 1106 76-77 -0.6341269545202906 1106 78-79 -0.6310216108156688 1106 80-81 -0.4263263360314724 1106 82-83 -0.36877613544969634 1106 84-85 -0.3031887924844163 1106 86-87 -0.6221217821814022 1106 88-89 -1.0961132947332466 1106 90-91 -1.0336923733440742 1106 92-93 -1.0066840571308653 1106 94-95 -1.3379655375587198 1106 96-97 -1.0671194463485634 1106 98-99 -0.952103153233832 1106 100-101 -1.4070587846927083 1107 1 -0.15983484605021303 1107 2 -0.10779114284305535 1107 3 -0.12196079917168134 1107 4 -0.03218952822248866 1107 5 -0.08411040366006972 1107 6 -0.06010081747790963 1107 7 -0.09159939301494546 1107 8 -0.033542135153837194 1107 9 -0.07954727041483522 1107 10-11 0.013732201772484132 1107 12-13 -0.09727486779882355 1107 14-15 -0.15700125387370178 1107 16-17 -0.11007921248608454 1107 18-19 -0.13804013941796 1107 20-21 -0.11492752280564389 1107 22-23 0.061160698136816904 1107 24-25 -0.03250844642887074 1107 26-27 -0.09981862998448321 1107 28-29 -0.13623627946505223 1107 30-31 -0.2832494931826801 1107 32-33 -0.20684186832856 1107 34-35 -0.3379062741216927 1107 36-37 -0.3336934120019279 1107 38-39 -0.33433091462031683 1107 40-41 -0.20605066489694934 1107 42-43 -0.21518003068850078 1107 44-45 -0.3132644900509334 1107 46-47 -0.2779153668576484 1107 48-49 -0.468071491200007 1107 50-51 -0.4851384773443925 1107 52-53 -0.35683373285750264 1107 54-55 -0.31022354939133123 1107 56-57 -0.3768414174051955 1107 58-59 -0.4084855205144038 1107 60-61 -0.3460951515270878 1107 62-63 -0.4498139013961193 1107 64-65 -0.6130685176900919 1107 66-67 -0.6396019366706334 1107 68-69 -0.6740383479963619 1107 70-71 -0.7744345260415137 1107 72-73 -0.6962516052031411 1107 74-75 -1.0871602727087328 1107 76-77 -1.0669150178145692 1107 78-79 -1.1780567271029732 1107 80-81 -1.2031917117969861 1107 82-83 -1.0753354826832258 1107 84-85 -1.2145638854080616 1107 86-87 -1.3968725698199016 1107 88-89 -1.407146251652076 1107 90-91 -1.59063350400357 1107 92-93 -1.6628394962614514 1107 94-95 -1.6256346585895507 1107 96-97 -1.5367304060285534 1107 98-99 -1.6124760867890018 1107 100-101 -1.3012222949054593 1108 1 0.027375780135745487 1108 2 -0.017979106038069403 1108 3 -0.05367538153536344 1108 4 0.06530501189422466 1108 5 0.022540987267575474 1108 6 -0.0039746494209111916 1108 7 7.509053379806119E-5 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46-47 -0.7504982574969503 2316 48-49 -0.6793591419994769 2316 50-51 -0.7475045569508154 2316 52-53 -0.6717163644226787 2316 54-55 -0.5003019258600716 2316 56-57 -0.5975873677362316 2316 58-59 -0.5158136549189223 2316 60-61 -0.5758507320886821 2316 62-63 -0.5272433102360452 2316 64-65 -0.3882541874883252 2316 66-67 -0.436507701724409 2316 68-69 -0.5803254754137299 2316 70-71 -0.6300883611238142 2316 72-73 -0.4799294111853989 2316 74-75 -0.5100145369643485 2316 76-77 -0.5315714174691344 2316 78-79 -0.539538029277626 2316 80-81 -0.4439333442257407 2316 82-83 -0.3671009813355184 2316 84-85 -0.3457503926176493 2316 86-87 -0.39994495356365434 2316 88-89 -0.5558341327396654 2316 90-91 -0.38121236460074925 2316 92-93 -0.4361903192797989 2316 94-95 -0.37081287019293185 2316 96-97 -0.26030667588436174 2316 98-99 -0.47842811881998415 2316 100-101 -0.21585344137393925 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7752.0 3 2397.0 4 2284.0 5 2642.0 6 3105.0 7 3789.0 8 4832.0 9 5794.0 10 7659.0 11 9653.0 12 12100.0 13 15073.0 14 17167.0 15 21045.0 16 24190.0 17 29689.0 18 29455.0 19 26786.0 20 27444.0 21 30099.0 22 32485.0 23 36400.0 24 40289.0 25 45522.0 26 51562.0 27 58983.0 28 67818.0 29 78102.0 30 91082.0 31 108087.0 32 129773.0 33 160802.0 34 204151.0 35 263567.0 36 321261.0 37 323449.0 38 148948.0 39 7943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.35185812617411 18.03046544797255 13.560577795230977 24.057098630622363 2 15.960945663425328 19.441845516843088 39.588297239734956 25.008911579996624 3 19.82032886904519 22.90031208101368 28.916608556343604 28.36275049359752 4 12.956632443766509 15.5951686565964 35.826286501717 35.6219123979201 5 13.938848369917492 37.03214338664901 33.86902240794193 15.159985835491568 6 33.93622113882771 34.85895358032114 16.536438761706215 14.668386519144933 7 29.94586551880987 31.986790173966938 19.61025460110425 18.457089706118936 8 27.156257840019745 34.4754045195228 19.10490685801584 19.263430782441617 9 26.562586093811287 16.380302919608226 19.732426046564022 37.32468494001647 10-11 25.528399051664685 26.039394273262573 26.501320079490416 21.930886595582326 12-13 26.305808326234526 23.2357749766316 27.20143205077093 23.256984646362948 14-15 22.97480494765335 24.814703842509797 25.34376279479305 26.8667284150438 16-17 22.633615845845565 28.043520234075963 26.37391659655899 22.948947323519484 18-19 22.767579707101316 26.3822908075725 28.530630846304827 22.319498639021358 20-21 22.851373393571183 25.56889336627148 29.202553305434957 22.377179934722374 22-23 22.693625316160997 25.579362153805622 28.962175044699062 22.764837485334315 24-25 22.579118503677346 25.822661066479785 28.97645302932786 22.621767400515015 26-27 22.09062933248854 26.1940930371551 28.84916857044412 22.866109059912244 28-29 22.314399893226188 26.631698515819547 28.693890178891028 22.360011412063237 30-31 22.32739868505506 26.158492770985525 28.649853575376344 22.864254968583076 32-33 22.09700223012398 26.050452065395202 28.776231710861 23.07631399361982 34-35 22.85721222002641 26.222923739385696 28.52199731647244 22.397866724115456 36-37 22.97235134438801 25.71891869618016 28.485375406023394 22.823354553408436 38-39 22.376801545747917 26.156583448465724 28.523066413120503 22.943548592665856 40-41 22.899656926187532 26.05631533586033 28.47541195574099 22.56861578221115 42-43 22.491085479059137 25.95883616452482 28.423861303025188 23.126217053390864 44-45 22.453823732414683 26.371422658256368 28.641734149894837 22.533019459434108 46-47 22.902061323780902 26.590795355873126 27.838373225997614 22.668770094348357 48-49 22.72420206350459 26.565332723241657 27.83078357018226 22.879681643071493 50-51 22.901378579003183 26.87160453544335 27.680112570356474 22.546904315196997 52-53 23.00349991875589 26.566905288532794 27.735760313363727 22.693834479347586 54-55 22.514607778620448 26.76032067879854 27.94183113524153 22.783240407339484 56-57 22.753569796844875 26.891168163889244 27.688869108502374 22.666392930763504 58-59 22.62112451019478 26.452265467036863 27.740609963629765 23.186000059138596 60-61 22.447960525645076 26.74603676253199 27.925618570889704 22.880384140933234 62-63 22.421664302822236 26.77699753351272 28.137593079560787 22.66374508410426 64-65 22.153704426422248 26.695881714128884 28.070465193761745 23.079948665687123 66-67 22.20467094585226 26.783984046848108 27.85134043993508 23.16000456736455 68-69 22.664792440384982 26.44123466423931 28.0349320752739 22.85904082010181 70-71 22.464786578215907 26.60629691639119 27.779520236883805 23.149396268509097 72-73 22.54196236789927 26.63681733469094 27.717869976358855 23.103350321050936 74-75 22.556147820021113 27.115406065022853 27.3481271893434 22.98031892561264 76-77 22.749240962133626 26.738641647892457 27.48337843152085 23.02873895845306 78-79 22.796829340788783 26.83456378972141 27.50395190761853 22.864654961871278 80-81 22.836093474009715 26.988190164287357 27.197443295337226 22.9782730663657 82-83 22.837745025188262 27.07947650491992 27.146588122426106 22.93619034746571 84-85 22.830765291592318 27.083481555580335 27.191893600447997 22.893859552379347 86-87 23.00032048041074 27.046487599936064 26.97285049792707 22.980341421726123 88-89 22.92257111856221 27.11915775390163 26.830420878305066 23.127850249231095 90-91 23.033735032684618 27.02379487644077 26.75902874740687 23.183441343467738 92-93 23.089199974052814 26.65708062353206 26.9589614272257 23.294757975189434 94-95 22.91938417455309 26.630703604988593 27.053926031024616 23.395986189433696 96-97 23.262436834797196 26.21951468239141 26.945455613442824 23.57259286936857 98-99 23.055811265361793 26.483605183334074 26.608330841947144 23.852252709356993 100-101 23.959941998757117 25.25378605051741 26.83314642456624 23.953125526159234 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1275.0 1 868.0 2 630.5 3 856.0 4 687.0 5 621.5 6 1273.0 7 2346.5 8 3525.0 9 4002.5 10 3250.0 11 2526.5 12 2400.0 13 2496.5 14 2694.5 15 2797.0 16 2878.0 17 3041.0 18 3302.5 19 3709.5 20 4179.0 21 4833.5 22 5858.5 23 7167.5 24 8842.5 25 11166.5 26 14069.0 27 17216.5 28 19919.0 29 22477.5 30 25615.0 31 30284.0 32 37149.0 33 40182.5 34 40430.0 35 43310.5 36 47927.5 37 53095.5 38 56527.5 39 58953.0 40 62567.5 41 67546.5 42 70898.5 43 73728.5 44 76646.5 45 81211.0 46 85900.5 47 89470.0 48 91475.0 49 90996.0 50 91786.0 51 90714.5 52 89951.0 53 92487.0 54 94036.5 55 89283.5 56 81401.0 57 74869.5 58 68902.5 59 60929.0 60 50117.5 61 41348.0 62 34590.0 63 26740.5 64 20084.0 65 15480.0 66 11575.0 67 8850.0 68 6762.5 69 4989.0 70 3749.5 71 2809.5 72 2046.5 73 1583.5 74 1212.5 75 901.0 76 652.5 77 401.5 78 229.0 79 157.0 80 110.0 81 74.0 82 46.5 83 29.0 84 24.0 85 15.0 86 13.5 87 11.5 88 5.5 89 6.0 90 6.0 91 2.5 92 2.5 93 3.5 94 2.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14075613724069871 2 0.05352238870461552 3 0.1555125003108212 4 0.13288879449889307 5 0.19590906330112884 6 0.2000261701245608 7 0.16684473493373292 8 0.07394486908619387 9 0.12595901073668084 10-11 0.1047212616771952 12-13 0.15589975293282715 14-15 0.14071537380680332 16-17 0.13761735283075555 18-19 0.13400978893101562 20-21 0.1359460520410455 22-23 0.19817143388232167 24-25 0.21541436642006148 26-27 0.14931645835872556 28-29 0.12799718243144914 30-31 0.09905514436573931 32-33 0.12599977417057623 34-35 0.09377627967628942 36-37 0.08639809814122819 38-39 0.05264597487586515 40-41 0.061960419520956274 42-43 0.06363172031066629 44-45 0.04184366489359317 46-47 0.05331857153513869 48-49 0.04437099779510586 50-51 0.056212775341709674 52-53 0.028269441406436304 54-55 0.053705824157144665 56-57 0.028269441406436304 58-59 0.05346124355377248 60-61 0.038888315936179134 62-63 0.05346124355377248 64-65 0.04149717570548256 66-67 0.04072267046147061 68-69 0.06842142379337178 70-71 0.05478605515537187 72-73 0.053379716685981744 74-75 0.044941685869640986 76-77 0.02313324873562019 78-79 0.02307210358477714 80-81 0.026373941730301786 82-83 0.022358743491608237 84-85 0.020361335230735304 86-87 0.02490645811006861 88-89 0.06681126815450483 90-91 0.11065234130897092 92-93 0.08340198574991878 94-95 0.07612571279959596 96-97 0.06811569803915654 98-99 0.056987280585721625 100-101 0.13172703663287513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2453179.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.003790509366475 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95638142375562 40.789799193436444 2 12.865476644541612 13.638380577408615 3 4.2205809217539185 6.711203610134206 4 1.919949380691694 4.070583790510819 5 1.0189151875336828 2.700318357342359 6 0.6258680543844936 1.9904027544660288 7 0.42890299496288 1.5913439145696646 8 0.3067364400428571 1.300655520769636 9 0.22468251300592168 1.0718122365435492 >10 1.2679395072401909 12.56235231863485 >50 0.10492950688680272 3.835102284269884 >100 0.053402565995005785 5.249757350508258 >500 0.003155916387837734 1.1393503612457634 >1k 0.0028480221060955325 2.4093388323774576 >5k 2.309207113050432E-4 0.9395988977825863 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9962 0.40608532846563583 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7964 0.3246399875426946 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5104 0.2080565666019479 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3445 0.14043002976953578 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3383 0.1379026968680231 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2922 0.11911075384225937 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2762 0.11258860441900082 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.076343389536597E-5 0.0 10-11 0.0 0.0 0.0 8.152686779073194E-5 0.0 12-13 0.0 0.0 0.0 1.2229030168609792E-4 4.076343389536597E-5 14-15 0.0 0.0 0.0 1.2229030168609792E-4 4.076343389536597E-5 16-17 0.0 0.0 0.0 2.0381716947682986E-4 4.076343389536597E-5 18-19 0.0 0.0 0.0 2.4458060337219584E-4 4.076343389536597E-5 20-21 0.0 0.0 0.0 4.891612067443917E-4 4.076343389536597E-5 22-23 0.0 0.0 0.0 7.541235270642705E-4 4.076343389536597E-5 24-25 0.0 0.0 0.0 0.0011413761490702472 4.076343389536597E-5 26-27 0.0 0.0 0.0 0.0017732093744484197 4.076343389536597E-5 28-29 0.0 4.076343389536597E-5 0.0 0.004952757218286965 4.076343389536597E-5 30-31 0.0 4.076343389536597E-5 0.0 0.015856975785297364 4.076343389536597E-5 32-33 0.0 4.076343389536597E-5 0.0 0.03711510656173072 4.076343389536597E-5 34-35 0.0 4.076343389536597E-5 0.0 0.06836027864252874 4.076343389536597E-5 36-37 0.0 6.114515084304896E-5 0.0 0.12379854874022646 4.076343389536597E-5 38-39 0.0 1.2229030168609792E-4 0.0 0.20440823926831267 4.076343389536597E-5 40-41 0.0 1.2229030168609792E-4 0.0 0.2879936604707606 4.076343389536597E-5 42-43 0.0 1.2229030168609792E-4 0.0 0.3743713768950411 4.076343389536597E-5 44-45 0.0 1.2229030168609792E-4 0.0 0.4750978220504904 6.114515084304896E-5 46-47 0.0 1.2229030168609792E-4 0.0 0.5953091886079247 8.152686779073194E-5 48-49 0.0 1.2229030168609792E-4 0.0 0.7196376619887909 8.152686779073194E-5 50-51 0.0 1.2229030168609792E-4 0.0 0.8536270692028588 8.152686779073194E-5 52-53 0.0 1.2229030168609792E-4 0.0 0.9770383653210793 8.152686779073194E-5 54-55 0.0 1.2229030168609792E-4 0.0 1.112332202419799 8.152686779073194E-5 56-57 0.0 1.2229030168609792E-4 0.0 1.2633811067190774 1.2229030168609792E-4 58-59 0.0 1.2229030168609792E-4 0.0 1.4207891067060334 1.6305373558146388E-4 60-61 0.0 1.2229030168609792E-4 0.0 1.5940948459121818 1.6305373558146388E-4 62-63 0.0 1.2229030168609792E-4 0.0 1.7593090434901 1.6305373558146388E-4 64-65 0.0 1.2229030168609792E-4 0.0 1.9241767518799078 1.6305373558146388E-4 66-67 0.0 1.426720186337809E-4 0.0 2.1206565032555718 1.6305373558146388E-4 68-69 2.0381716947682984E-5 1.6305373558146388E-4 0.0 2.3346237677723476 2.0381716947682986E-4 70-71 4.076343389536597E-5 1.6305373558146388E-4 0.0 2.5573551705766273 2.0381716947682986E-4 72-73 4.076343389536597E-5 1.6305373558146388E-4 0.0 2.7814317666994537 2.0381716947682986E-4 74-75 4.076343389536597E-5 1.6305373558146388E-4 0.0 3.0058752337273393 2.0381716947682986E-4 76-77 4.076343389536597E-5 1.6305373558146388E-4 0.0 3.244626666052498 2.0381716947682986E-4 78-79 4.076343389536597E-5 1.6305373558146388E-4 0.0 3.5085699005249924 2.0381716947682986E-4 80-81 4.076343389536597E-5 1.6305373558146388E-4 0.0 3.7882885839149933 2.0381716947682986E-4 82-83 4.076343389536597E-5 1.6305373558146388E-4 0.0 4.074509035011306 2.0381716947682986E-4 84-85 4.076343389536597E-5 1.6305373558146388E-4 0.0 4.3597919271280245 2.0381716947682986E-4 86-87 4.076343389536597E-5 1.6305373558146388E-4 0.0 4.6510874257443096 2.0381716947682986E-4 88-89 4.076343389536597E-5 1.6305373558146388E-4 0.0 4.957852647523886 2.0381716947682986E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4865 0.0 48.73454 1 GTATCAA 8395 0.0 40.208317 1 ATCAACG 10265 0.0 32.715553 3 TCAACGC 10330 0.0 32.37155 4 TATCAAC 10535 0.0 31.877092 2 CAACGCA 10535 0.0 31.83194 5 AACGCAG 10880 0.0 30.858713 6 CGCAGAG 12610 0.0 26.543188 8 ACGCAGA 12660 0.0 26.478077 7 TACATGG 8215 0.0 25.535215 2 GTACATG 8440 0.0 25.327614 1 GCAGAGT 13740 0.0 23.7369 9 ACATGGG 8560 0.0 23.672512 3 TAAGGTG 1320 0.0 23.423292 5 GAGTACT 8565 0.0 22.367172 12-13 GTAAGGT 1260 0.0 22.273579 4 GTACTTT 8955 0.0 21.259912 14-15 GAGTACA 6295 0.0 20.949537 1 CAGAGTA 13080 0.0 20.735397 10-11 AGAGTAC 12235 0.0 20.710363 10-11 >>END_MODULE