FastQCFastQC Report
Thu 26 May 2016
SRR937956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453179
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100330.4089795322722068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81630.3327519108878724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52560.21425260855404354No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34830.14197904025755967No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34140.13916636331877943No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32920.13419322438354478No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28920.1178878508253984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.046.5316731
GTATCAA81850.040.3355641
ATCAACG103000.031.5399673
TCAACGC103800.031.2936944
TATCAAC105300.031.078482
CAACGCA105850.030.6427635
AACGCAG108550.029.9243266
CGCAGAG126750.025.4401638
ACGCAGA126900.025.4100917
GGACCGT1951.5752448E-824.3536196
GTACATG82650.024.024561
TACATGG82250.023.791962
GCAGAGT140700.022.5465689
GGACCGA8750.022.2522496
TATACCG1503.0401821E-522.1617935
AGGACCG10500.022.161795
TAAGGTG12900.022.0881655
ACATGGG85700.021.8906483
GAGTACT86100.021.26317612-13
GTAAGGT12450.020.597884
Produced by FastQC (version 0.11.5)