##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937956_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2453179 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53106194044544 34.0 31.0 34.0 31.0 34.0 2 32.74038543457286 34.0 31.0 34.0 31.0 34.0 3 32.799001214342695 34.0 31.0 34.0 31.0 34.0 4 36.24656292916253 37.0 35.0 37.0 35.0 37.0 5 36.222265884389195 37.0 37.0 37.0 35.0 37.0 6 36.25339406541471 37.0 37.0 37.0 35.0 37.0 7 36.21283893266655 37.0 37.0 37.0 35.0 37.0 8 36.19752044184301 37.0 37.0 37.0 35.0 37.0 9 37.89292668818704 39.0 38.0 39.0 35.0 39.0 10-11 37.87281136027987 39.0 38.0 39.0 35.0 39.0 12-13 37.78985532649676 39.0 38.0 39.0 35.0 39.0 14-15 39.08583169022725 40.0 38.5 41.0 36.0 41.0 16-17 38.96687481834795 40.0 38.0 41.0 36.0 41.0 18-19 39.211786216986205 40.5 39.0 41.0 36.0 41.0 20-21 39.187686671050095 40.5 39.0 41.0 36.0 41.0 22-23 39.09572680998818 40.0 39.0 41.0 35.5 41.0 24-25 38.99409317461139 40.0 38.5 41.0 35.0 41.0 26-27 38.82035432392011 40.0 38.0 41.0 35.0 41.0 28-29 38.707567609212376 40.0 38.0 41.0 34.5 41.0 30-31 38.47471627630923 40.0 38.0 41.0 34.0 41.0 32-33 38.26585768914539 40.0 38.0 41.0 34.0 41.0 34-35 38.111835499977786 40.0 38.0 41.0 34.0 41.0 36-37 38.11647967800148 40.0 38.0 41.0 34.0 41.0 38-39 37.971875676418236 40.0 38.0 41.0 33.0 41.0 40-41 37.79712731928653 40.0 37.0 41.0 33.0 41.0 42-43 37.563294199078015 40.0 37.0 41.0 33.0 41.0 44-45 37.30170403382713 40.0 37.0 41.0 32.0 41.0 46-47 36.92848035141341 40.0 36.0 41.0 31.0 41.0 48-49 36.53922257609412 39.0 36.0 41.0 30.5 41.0 50-51 36.16040574291562 39.0 35.0 40.5 29.5 41.0 52-53 35.64433109039332 38.5 35.0 40.0 28.0 41.0 54-55 35.330853150137024 38.0 34.5 40.0 27.5 41.0 56-57 34.974075067494056 38.0 34.0 40.0 27.0 41.0 58-59 34.52501427739273 38.0 34.0 40.0 26.0 41.0 60-61 34.04231081384603 37.0 33.0 40.0 25.0 41.0 62-63 33.56772620342829 36.5 33.0 39.0 23.5 41.0 64-65 33.07036298614981 36.0 32.0 39.0 22.5 40.0 66-67 32.51651448997403 35.0 31.5 38.5 21.0 40.0 68-69 31.91590890840008 35.0 31.0 38.0 18.5 40.0 70-71 31.28443806994924 35.0 30.5 37.0 16.0 39.5 72-73 30.69658308668059 34.0 30.0 36.5 10.5 39.0 74-75 30.090867197216348 34.0 29.0 36.0 7.5 39.0 76-77 28.729772470741025 32.5 27.5 35.0 7.0 36.5 78-79 29.413267845518 34.0 29.0 35.0 4.0 37.0 80-81 29.078764533692812 34.0 29.0 35.0 2.0 37.0 82-83 28.51349106608201 33.5 27.5 35.0 2.0 36.0 84-85 27.915185968899948 33.0 26.5 35.0 2.0 36.0 86-87 27.26755161364091 33.0 26.0 35.0 2.0 35.5 88-89 26.51026117539731 32.0 25.0 34.5 2.0 35.0 90-91 25.94672830641384 32.0 23.5 34.0 2.0 35.0 92-93 25.1090395360469 31.5 19.5 34.0 2.0 35.0 94-95 24.263829708309096 31.0 14.0 34.0 2.0 35.0 96-97 23.185259412378795 31.0 2.0 34.0 2.0 35.0 98-99 21.690893734211812 30.0 2.0 34.0 2.0 35.0 100-101 17.26215270063864 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15552054235604373 1101 2 0.13182535011078045 1101 3 0.0575886677378179 1101 4 0.06021304910929359 1101 5 0.076776883862955 1101 6 0.08413291240617582 1101 7 0.06017805902944673 1101 8 0.07287029210258567 1101 9 -0.0035398916946292047 1101 10-11 0.060899754842601794 1101 12-13 0.1104172398587977 1101 14-15 0.10193207814376848 1101 16-17 0.13849827893435673 1101 18-19 0.21154318142141904 1101 20-21 0.21714506517747623 1101 22-23 0.20438546414643355 1101 24-25 0.2501288952209677 1101 26-27 0.202514619399345 1101 28-29 0.24948085709370105 1101 30-31 0.22877429407172656 1101 32-33 0.17764670819673967 1101 34-35 0.297958655522514 1101 36-37 0.2791897912572594 1101 38-39 0.13750932801893612 1101 40-41 0.13916462860123602 1101 42-43 0.2591181124899222 1101 44-45 0.13214889632370586 1101 46-47 0.08800531682648938 1101 48-49 -0.029528925265303485 1101 50-51 0.008789846900889131 1101 52-53 -0.025227457066939962 1101 54-55 -0.2699160641705163 1101 56-57 -0.012524247924822873 1101 58-59 0.07039647500825907 1101 60-61 0.10300121986880839 1101 62-63 -3.371903645259522E-4 1101 64-65 0.019555501150790633 1101 66-67 -0.12898415464181312 1101 68-69 0.03251432829923928 1101 70-71 0.05322353344237385 1101 72-73 -0.09128384108582566 1101 74-75 -0.14441018130637318 1101 76-77 -0.05756748641492848 1101 78-79 -0.007146957289105416 1101 80-81 -0.06976155752353463 1101 82-83 -0.14898897975435332 1101 84-85 -0.22576764348761458 1101 86-87 -0.2669358975927416 1101 88-89 -0.3789629180811467 1101 90-91 -0.4950624481165846 1101 92-93 -0.43142202363692306 1101 94-95 -0.7430431551451626 1101 96-97 -0.7486185190178638 1101 98-99 -0.5540826461093822 1101 100-101 -1.1670699823801627 1102 1 0.08513064953113059 1102 2 0.10612501305612909 1102 3 0.1225123441526037 1102 4 0.03782139607469048 1102 5 -0.002035867453216156 1102 6 0.040912111319443056 1102 7 0.04490505114358001 1102 8 0.10189015504857224 1102 9 0.18741607436393792 1102 10-11 0.18972159419796242 1102 12-13 0.15926242112531952 1102 14-15 0.18936634929896456 1102 16-17 0.14286213213541998 1102 18-19 0.25294796906423755 1102 20-21 0.12758514948896504 1102 22-23 0.1838535109195405 1102 24-25 0.23421589804942045 1102 26-27 0.26955150408328876 1102 28-29 0.188848329566504 1102 30-31 0.21629785122593148 1102 32-33 0.13142557866508042 1102 34-35 0.3164733829291748 1102 36-37 0.4237702905573215 1102 38-39 0.31115847868587565 1102 40-41 0.20364506380670377 1102 42-43 0.29554169802431574 1102 44-45 0.06098062091466261 1102 46-47 0.10345179465326737 1102 48-49 0.19556012157476488 1102 50-51 0.15241881255447964 1102 52-53 0.08738993010585006 1102 54-55 0.07723268311502807 1102 56-57 0.15587966917950524 1102 58-59 0.24683102996538508 1102 60-61 -0.08448120195013331 1102 62-63 0.14630581551241306 1102 64-65 -0.028994717440468776 1102 66-67 -0.17499291104728343 1102 68-69 -0.19636363368405796 1102 70-71 -0.14247160589161467 1102 72-73 -0.18859665139452986 1102 74-75 -0.3184885425256354 1102 76-77 -0.2143648614065583 1102 78-79 -0.15517931708617283 1102 80-81 -0.2199638859748383 1102 82-83 -0.1264075939718765 1102 84-85 -0.2086684180436542 1102 86-87 -0.4900278079623561 1102 88-89 -0.4364621448006183 1102 90-91 -0.634615507915882 1102 92-93 -0.5263547243151798 1102 94-95 -0.7309082312455324 1102 96-97 -0.911944294929139 1102 98-99 -1.0588508373344787 1102 100-101 -1.2874507871658913 1103 1 0.10859665878619751 1103 2 0.0878171843634803 1103 3 0.11281604905215659 1103 4 0.05431211774252631 1103 5 0.07647035809058877 1103 6 0.03395017588803739 1103 7 0.05900593922378761 1103 8 0.021700344038222852 1103 9 0.10108467628397477 1103 10-11 0.08937862586805778 1103 12-13 0.10569235811527733 1103 14-15 0.1888195178262393 1103 16-17 0.23301194332380248 1103 18-19 0.19024563838474506 1103 20-21 0.1578279847423829 1103 22-23 0.1686166780670888 1103 24-25 0.13227087332568033 1103 26-27 0.1555406823633021 1103 28-29 0.13101629887758293 1103 30-31 0.20302995559447368 1103 32-33 0.20286982876147874 1103 34-35 0.2567791311131771 1103 36-37 0.18596283188123408 1103 38-39 0.10465636317839966 1103 40-41 0.11363787791920998 1103 42-43 0.05787018857119364 1103 44-45 0.17896057325960868 1103 46-47 0.10139151654809808 1103 48-49 0.057752116674116394 1103 50-51 -0.00324304774069617 1103 52-53 -0.04346150045626729 1103 54-55 0.0462708545008752 1103 56-57 0.0735751599137231 1103 58-59 0.20152342259483902 1103 60-61 0.07143940694053441 1103 62-63 0.09719871058752716 1103 64-65 -0.16736958204758423 1103 66-67 -0.07411221498726661 1103 68-69 0.06835984923793248 1103 70-71 -0.1329952167515316 1103 72-73 -0.2391631874123732 1103 74-75 -0.25041773482945473 1103 76-77 -0.31168862407644227 1103 78-79 -0.4461000331987037 1103 80-81 -0.3256807935669954 1103 82-83 -0.34780879348100413 1103 84-85 -0.2947667603128181 1103 86-87 -0.5263101672154598 1103 88-89 -0.7021933108767549 1103 90-91 -0.7718005133639672 1103 92-93 -0.6289452043899217 1103 94-95 -0.5917177724533218 1103 96-97 -0.43294733719761425 1103 98-99 -0.4761866290829282 1103 100-101 -1.2466319318178876 1104 1 0.17859359531415464 1104 2 0.13181106044131496 1104 3 0.11127316969817969 1104 4 0.14484659351295193 1104 5 0.03290521700277793 1104 6 0.10017008936289784 1104 7 0.11452107659085442 1104 8 0.056362151456511356 1104 9 0.156637776529017 1104 10-11 0.19123255529034822 1104 12-13 0.11467407285126541 1104 14-15 0.15388365998040143 1104 16-17 0.20524889740911334 1104 18-19 0.20985223720174417 1104 20-21 0.15963301571140676 1104 22-23 0.11291550371095838 1104 24-25 0.18512757044952366 1104 26-27 0.14171237353962596 1104 28-29 0.03024487808760057 1104 30-31 0.10360036200124512 1104 32-33 0.09190065348516896 1104 34-35 0.0618206768558025 1104 36-37 0.04223849223699716 1104 38-39 0.037979100902504115 1104 40-41 0.11520710464370865 1104 42-43 0.12202867999818778 1104 44-45 0.03862777291752195 1104 46-47 -0.042490624014284606 1104 48-49 -0.020584187584937297 1104 50-51 -0.0940058673890789 1104 52-53 -0.012882968860388644 1104 54-55 -0.08669422002913763 1104 56-57 -0.13802803319871515 1104 58-59 -0.14159196226753323 1104 60-61 -0.35836874265057617 1104 62-63 -0.31829186234218554 1104 64-65 -0.3570409972847912 1104 66-67 -0.4824060839258095 1104 68-69 -0.5169002418049864 1104 70-71 -0.5375289665055298 1104 72-73 -0.642999345674891 1104 74-75 -0.5230623082343655 1104 76-77 -0.6298389782266511 1104 78-79 -0.5755584521373791 1104 80-81 -0.5650992551600851 1104 82-83 -0.6294746498951973 1104 84-85 -0.554101378717899 1104 86-87 -0.7854639425575698 1104 88-89 -0.8434295710535444 1104 90-91 -0.888120943473929 1104 92-93 -0.8700327105234749 1104 94-95 -0.4974171336570734 1104 96-97 -0.8019335734823443 1104 98-99 -1.1373375351113957 1104 100-101 -1.885079431424094 1105 1 0.12334166553876003 1105 2 0.14082906460077282 1105 3 0.08031448722174162 1105 4 0.03563579759937596 1105 5 0.05571589995907544 1105 6 0.011706170403378735 1105 7 0.07872667583242787 1105 8 0.04350954478672264 1105 9 0.09908582838745161 1105 10-11 0.13057056615929952 1105 12-13 0.1718839111758399 1105 14-15 0.11791223265259987 1105 16-17 0.07584459845025293 1105 18-19 0.11655047670303276 1105 20-21 0.1316368078487713 1105 22-23 0.18310242465890525 1105 24-25 0.06541992173393396 1105 26-27 0.13930114957739903 1105 28-29 0.03307809941911444 1105 30-31 0.0968802529507542 1105 32-33 0.2105312799840604 1105 34-35 0.2607974792092165 1105 36-37 0.26373774538127037 1105 38-39 0.2650471268784358 1105 40-41 0.18470927514338342 1105 42-43 0.23390230585266636 1105 44-45 0.13909074915084574 1105 46-47 0.14637628916255352 1105 48-49 0.26963110007925906 1105 50-51 0.21524282866559474 1105 52-53 0.2085860278687406 1105 54-55 0.23141801322340427 1105 56-57 0.24057871727291058 1105 58-59 0.3676643775130728 1105 60-61 0.35506744126171697 1105 62-63 0.3634581580941685 1105 64-65 0.5551320609168755 1105 66-67 0.3947441121039148 1105 68-69 0.3251588824151206 1105 70-71 0.29834325162942577 1105 72-73 0.18499399579051712 1105 74-75 0.19841544693072777 1105 76-77 0.1607401826408683 1105 78-79 0.06493216890473619 1105 80-81 0.08166320225533852 1105 82-83 0.07126312890174447 1105 84-85 0.010019927212674418 1105 86-87 0.004236986048969271 1105 88-89 -0.04692413392266204 1105 90-91 -0.29278085605943005 1105 92-93 -0.49943700948233527 1105 94-95 -0.6791992683586301 1105 96-97 -0.878790176122191 1105 98-99 -0.8589254342439396 1105 100-101 -1.392160228792104 1106 1 0.17852071291719085 1106 2 0.1986512071805393 1106 3 0.24627193568937855 1106 4 0.11513373817632555 1106 5 0.1673107402504641 1106 6 0.17725929821548903 1106 7 0.18853384958035946 1106 8 0.20214711284281606 1106 9 0.1942598695197688 1106 10-11 0.20551570799513996 1106 12-13 0.15421876353836694 1106 14-15 0.24646417206610494 1106 16-17 0.3017750428208785 1106 18-19 0.32557041435109824 1106 20-21 0.3495985872651488 1106 22-23 0.3174575546575511 1106 24-25 0.36638479379394084 1106 26-27 0.35350103136136823 1106 28-29 0.3290234689533804 1106 30-31 0.331872821560502 1106 32-33 0.3603020609740426 1106 34-35 0.34909572983032433 1106 36-37 0.3076905587824186 1106 38-39 0.24805142819518977 1106 40-41 0.24580087853016863 1106 42-43 0.1549279936656589 1106 44-45 0.08094928292896952 1106 46-47 -0.029454459169876657 1106 48-49 -0.03210439857248559 1106 50-51 0.021725488495071943 1106 52-53 -0.09908327604471623 1106 54-55 -0.3318724005170992 1106 56-57 -0.21489220517082686 1106 58-59 -0.1553156177769992 1106 60-61 -0.3649432340512533 1106 62-63 -0.14699277055668603 1106 64-65 -0.18406181649091735 1106 66-67 -0.401955898345264 1106 68-69 -0.5144624753848817 1106 70-71 -0.8980343550106369 1106 72-73 -0.7908588595632793 1106 74-75 -0.7438785990674752 1106 76-77 -0.8452899265763953 1106 78-79 -0.872470454929676 1106 80-81 -0.8936102697868833 1106 82-83 -0.8501232113763919 1106 84-85 -0.7698477503725982 1106 86-87 -0.940881261172251 1106 88-89 -1.13652872625671 1106 90-91 -1.3920193530003182 1106 92-93 -1.256073794630158 1106 94-95 -1.295277506159998 1106 96-97 -1.26169060952898 1106 98-99 -1.6593785696979886 1106 100-101 -1.9415341361126082 1107 1 -0.029773031994139387 1107 2 -0.07069213240670535 1107 3 -0.07331778430070557 1107 4 -0.06390622784017097 1107 5 -0.13118194975626096 1107 6 -0.034196682527188216 1107 7 -0.062450256051697295 1107 8 -0.04620610320022678 1107 9 -0.11254107918638567 1107 10-11 -0.03631975520709574 1107 12-13 -0.06851885549239256 1107 14-15 -0.16302943832718597 1107 16-17 -0.14353541064649988 1107 18-19 -0.05667338188948179 1107 20-21 -0.06338930611161686 1107 22-23 -0.04130965834114164 1107 24-25 -0.049089559280510286 1107 26-27 -0.019619787074681483 1107 28-29 -0.12028252417911744 1107 30-31 -0.22783123876021705 1107 32-33 -0.24443492086601992 1107 34-35 -0.060567975079592884 1107 36-37 -0.10098833250577144 1107 38-39 -0.15309321409304033 1107 40-41 -0.24241693448976065 1107 42-43 -0.14319441774713937 1107 44-45 -0.287645711584112 1107 46-47 -0.2328875010474647 1107 48-49 -0.28133127386011125 1107 50-51 -0.4207105269391036 1107 52-53 -0.44399070639622806 1107 54-55 -0.22453408765646543 1107 56-57 -0.3691284613214876 1107 58-59 -0.3493079714886349 1107 60-61 -0.5322795999403169 1107 62-63 -0.5453097021294866 1107 64-65 -0.6584961327498959 1107 66-67 -0.7165673890174986 1107 68-69 -0.7205032201900075 1107 70-71 -1.05113588072836 1107 72-73 -1.083898082190629 1107 74-75 -0.9719234856121304 1107 76-77 -0.9575604494267616 1107 78-79 -0.9885200496055901 1107 80-81 -0.8195596839254335 1107 82-83 -0.7556745583267279 1107 84-85 -1.0868187935953628 1107 86-87 -1.0884884331075426 1107 88-89 -1.1659352539214076 1107 90-91 -1.2732592198982609 1107 92-93 -1.291898779585381 1107 94-95 -1.432095800612899 1107 96-97 -1.8272630191717987 1107 98-99 -1.8162768669828502 1107 100-101 -2.120246302914973 1108 1 -0.0595084514205837 1108 2 -0.07441129749209097 1108 3 -0.049392396738504374 1108 4 -0.01272653502573462 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-0.2593971153276726 2316 50-51 -0.12421784120387969 2316 52-53 0.009643658872128924 2316 54-55 -0.051157880606510275 2316 56-57 0.1418001493607406 2316 58-59 4.278513988609234E-4 2316 60-61 0.015567860348070894 2316 62-63 0.037068284419596864 2316 64-65 0.35300950669217457 2316 66-67 0.20925026466056096 2316 68-69 0.46066457610968214 2316 70-71 0.7692153086574507 2316 72-73 0.7034329308154135 2316 74-75 0.9936930584958965 2316 76-77 0.8700174690826472 2316 78-79 0.9886976511768637 2316 80-81 1.0547367596932276 2316 82-83 1.196888443792968 2316 84-85 1.3313865292005431 2316 86-87 1.5174652999152514 2316 88-89 1.6057349891895747 2316 90-91 1.5744232441846435 2316 92-93 1.767805771221937 2316 94-95 2.209992886483114 2316 96-97 2.5179371744795773 2316 98-99 2.8328874226568033 2316 100-101 3.087582969845606 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 22.0 5 42.0 6 81.0 7 241.0 8 549.0 9 993.0 10 1792.0 11 2962.0 12 4653.0 13 7133.0 14 9544.0 15 13127.0 16 17063.0 17 23035.0 18 24261.0 19 20014.0 20 19253.0 21 19706.0 22 21510.0 23 23901.0 24 26962.0 25 30420.0 26 35274.0 27 41244.0 28 50253.0 29 59908.0 30 72474.0 31 88965.0 32 110697.0 33 142562.0 34 194309.0 35 274604.0 36 389156.0 37 472889.0 38 242120.0 39 11452.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76992754435579 18.440497539899933 13.862253149168613 23.92732176657566 2 16.282726632657347 19.703309243058197 39.09714263815624 24.916821486128217 3 19.864481321643442 22.87722599682178 29.10361663077785 28.154676050756926 4 13.222853467157078 15.92775409193874 35.59465949664455 35.25473294425963 5 13.957367570590701 36.96903091364756 33.9608243421943 15.112777173567437 6 34.01904010292124 35.117510714847676 16.31253521000449 14.550913972226592 7 29.969276599873062 32.01914740016933 19.4922588200861 18.519317179871507 8 27.157577983506297 34.66583563612765 18.927685260635283 19.248901119730768 9 26.43639131102948 16.38408774899834 19.689716893875254 37.48980404609692 10-11 25.53796522797562 26.07583873822497 26.432111150470472 21.954084883328935 12-13 26.29164227631671 23.25720418921585 27.19918749561789 23.25196603884955 14-15 22.883919192199183 24.848288689899924 25.352450840317807 26.91534127758309 16-17 22.585235784660025 28.142168278839858 26.366827863603177 22.905768072896944 18-19 22.67767659840558 26.46940969248473 28.53633998986621 22.31657371924348 20-21 22.803859808028683 25.57216574901383 29.23072470455682 22.393249738400662 22-23 22.722924010029434 25.602473362115035 28.97678889310564 22.69781373474989 24-25 22.621695135659568 25.859017592991744 28.990617003127667 22.52867026822102 26-27 22.1159126740959 26.252933960060226 28.87577296112964 22.75538040471423 28-29 22.326302289498766 26.67458477814748 28.711782916283806 22.287330016069948 30-31 22.34553303965117 26.16509970728939 28.684966871583796 22.804400381475645 32-33 22.07227845990855 26.079507447275557 28.832221374795726 23.01599271802017 34-35 22.872991827070464 26.23604959852848 28.55056470181526 22.34039387258579 36-37 22.93694195712021 25.748478340491786 28.53622908081228 22.77835062157572 38-39 22.38031111802294 26.14498553984609 28.60621808019622 22.868485261934747 40-41 22.89973040580988 26.01546227750123 28.533284905436172 22.55152241125272 42-43 22.463235961038038 26.011791967789467 28.42994638847292 23.09502568269958 44-45 22.512181645159536 26.296129694598253 28.698217435054158 22.49347122518806 46-47 22.91094831537804 26.553531716939244 27.895526473533394 22.639993494149323 48-49 22.710172369163917 26.53535330888872 27.875051209356076 22.879423112591287 50-51 22.882278676582395 26.854011274837724 27.735721684597237 22.527988363982647 52-53 23.022350266625928 26.559615421070433 27.758713575001064 22.65932073730258 54-55 22.591565349776406 26.784450885114218 27.92677177247264 22.69721199263674 56-57 22.753993225963946 26.932776406866175 27.652948377278953 22.66028198989093 58-59 22.6247530137823 26.481387249109705 27.735767355496243 23.15809238161175 60-61 22.453823969853477 26.794540997449946 27.92093875244055 22.83069628025603 62-63 22.406899793815104 26.82383117383503 28.07123551517479 22.698033517175077 64-65 22.126710005284135 26.794853185812418 27.971246305997166 23.107190502906274 66-67 22.17835005922742 26.815395337677835 27.771249047275326 23.235005555819424 68-69 22.59689341647731 26.51946227581658 27.95095361736441 22.9326906903417 70-71 22.431932112215925 26.641446031979974 27.664948801418486 23.26167305438561 72-73 22.40367411547977 26.7558630001064 27.622273142895814 23.21818974151802 74-75 22.372957299497138 27.166948390342625 27.29056433614731 23.169529974012928 76-77 22.490249338063993 26.77901163392798 27.64185245860199 23.08888656940604 78-79 22.53073696545937 26.821618566827443 27.663717434800283 22.983927032912906 80-81 22.582443577932747 27.0478779081441 27.373720098602682 22.995958415320477 82-83 22.501160145790973 27.092707270545997 27.348979194039174 23.057153389623853 84-85 22.512049403676325 27.108620160310785 27.340536269306465 23.03879416670642 86-87 22.614234740344404 27.146028123367017 27.13399896059878 23.105738175689797 88-89 22.474854710100093 27.267673259747372 26.92860403250188 23.328867997650654 90-91 22.510176545687113 27.16425146577733 27.007591990549866 23.31797999798569 92-93 22.472184076015512 26.824364987979866 27.222113944984656 23.481336991019965 94-95 22.253078228026766 26.801733209229813 27.351576224798606 23.593612337944812 96-97 22.548957653408394 26.441556387173264 27.194925111597723 23.81456084782062 98-99 22.26863049064004 26.665790141538427 26.99807470236366 24.06750466545788 100-101 22.689839093655575 25.93666686167501 27.100581294731736 24.272912749937674 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1237.0 1 818.0 2 590.5 3 797.0 4 661.5 5 625.5 6 1257.0 7 2416.0 8 3593.0 9 4033.0 10 3327.0 11 2479.5 12 2360.0 13 2509.0 14 2594.0 15 2727.5 16 2870.5 17 3002.5 18 3190.5 19 3514.5 20 4031.5 21 4741.0 22 5549.0 23 6738.5 24 8518.5 25 10850.0 26 13782.5 27 16919.0 28 19537.0 29 22274.0 30 25729.0 31 30074.5 32 37323.0 33 40478.5 34 40439.5 35 43489.0 36 48346.5 37 53163.5 38 56230.5 39 59241.0 40 63137.0 41 68361.0 42 71339.0 43 73911.0 44 77603.0 45 82620.5 46 86773.5 47 90625.5 48 93105.0 49 92800.5 50 93546.5 51 91591.5 52 90484.0 53 93870.0 54 96139.5 55 90591.0 56 81768.5 57 74420.5 58 68020.5 59 60256.5 60 48883.5 61 39862.0 62 33732.5 63 25658.0 64 18341.0 65 13949.0 66 10453.0 67 7959.5 68 5953.0 69 4273.5 70 3182.0 71 2313.5 72 1720.5 73 1406.0 74 1034.5 75 732.0 76 522.0 77 309.0 78 188.0 79 115.0 80 72.5 81 51.0 82 25.0 83 13.0 84 9.0 85 4.5 86 4.5 87 2.5 88 1.0 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009375589795934173 2 0.013125825714307843 3 0.009497880097620271 4 8.152686779073194E-5 5 9.375589795934174E-4 6 0.0016713007897100048 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.375589795934174E-4 14-15 0.0 16-17 0.00293496724046635 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0029145855235186667 26-27 0.017772857178379564 28-29 0.006236805385990993 30-31 0.002996112391309399 32-33 0.0 34-35 0.008274977080759293 36-37 7.541235270642705E-4 38-39 0.002384660882878909 40-41 0.0019362631100298836 42-43 0.002792295221832569 44-45 2.0381716947682984E-5 46-47 1.0190858473841493E-4 48-49 5.706880745351236E-4 50-51 0.0043209239929087925 52-53 0.008580702834974538 54-55 0.007765434157067218 56-57 0.017589421725850415 58-59 0.02498798497785934 60-61 0.0038113810692167183 62-63 0.021441566228962504 64-65 0.022623705811928116 66-67 0.014083766410848944 68-69 0.03287570943661266 70-71 0.017344841122478222 72-73 0.005136192670816113 74-75 0.017161405669949076 76-77 0.021400802795067137 78-79 0.027556081313267398 80-81 0.012412465621138938 82-83 0.03672785393972474 84-85 0.023153630452567873 86-87 0.03283494600271729 88-89 0.02374470024405068 90-91 0.029594253008035695 92-93 0.025558673052394464 94-95 0.015816212351401997 96-97 0.016142319822564925 98-99 0.0126978096584065 100-101 0.011923304414394547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2453179.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.6223431912001 #Duplication Level Percentage of deduplicated Percentage of total 1 74.82358922863142 37.877454127291635 2 13.922371271246517 14.095661130566919 3 4.677899087504306 7.104186390643343 4 2.1359175659687395 4.325006082103292 5 1.1379647824537649 2.8803221878436926 6 0.6951605311068337 2.111439298720024 7 0.4691709439742361 1.6625372778842167 8 0.3348077758643515 1.3559003306310082 9 0.2333726797850916 1.0632484698777858 >10 1.3864792426273735 13.159117194556066 >50 0.11771028551832473 4.115897842982685 >100 0.058894778832903706 5.606325755857937 >500 0.003371044727946535 1.1598930423428995 >1k 0.0030499928490935577 2.5301327508921374 >5k 1.6052593942597672E-4 0.5452273351033643 >10k+ 8.026296971298836E-5 0.40765078270303706 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10033 0.4089795322722068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8163 0.3327519108878724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5256 0.21425260855404354 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3483 0.14197904025755967 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3414 0.13916636331877943 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3292 0.13419322438354478 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2892 0.1178878508253984 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.076343389536597E-5 0.0 8 0.0 0.0 0.0 4.076343389536597E-5 0.0 9 0.0 0.0 0.0 8.152686779073194E-5 0.0 10-11 0.0 0.0 0.0 1.0190858473841493E-4 0.0 12-13 0.0 0.0 0.0 1.2229030168609792E-4 0.0 14-15 0.0 0.0 0.0 1.2229030168609792E-4 0.0 16-17 0.0 0.0 0.0 2.0381716947682986E-4 0.0 18-19 0.0 0.0 0.0 2.4458060337219584E-4 0.0 20-21 0.0 0.0 0.0 4.891612067443917E-4 0.0 22-23 0.0 0.0 0.0 7.745052440119534E-4 0.0 24-25 0.0 0.0 0.0 0.0012025212999132963 0.0 26-27 0.0 0.0 0.0 0.0018343545252914686 0.0 28-29 0.0 0.0 0.0 0.005075047519973064 0.0 30-31 0.0 0.0 0.0 0.016121938105617243 0.0 32-33 0.0 0.0 0.0 0.0373800688820506 0.0 34-35 0.0 0.0 0.0 0.06891058500011618 0.0 36-37 0.0 0.0 0.0 0.12487877973845365 0.0 38-39 0.0 0.0 0.0 0.20593686803938888 0.0 40-41 0.0 0.0 0.0 0.29043946650448255 0.0 42-43 0.0 0.0 0.0 0.37740825272024586 0.0 44-45 0.0 0.0 0.0 0.47837927847906736 0.0 46-47 0.0 0.0 0.0 0.5991817148279844 0.0 48-49 0.0 0.0 0.0 0.7237140053783274 0.0 50-51 0.0 0.0 0.0 0.8580295200635584 0.0 52-53 0.0 0.0 0.0 0.9822153214257907 0.0 54-55 0.0 0.0 0.0 1.118507862654947 0.0 56-57 0.0 0.0 0.0 1.2705962345185573 0.0 58-59 0.0 0.0 0.0 1.4295328632765893 0.0 60-61 0.0 0.0 0.0 1.6043264678199187 0.0 62-63 0.0 0.0 0.0 1.7699686814537383 0.0 64-65 0.0 0.0 0.0 1.9358758574078778 0.0 66-67 0.0 0.0 0.0 2.134454925629153 0.0 68-69 0.0 0.0 0.0 2.3506641790101743 0.0 70-71 0.0 0.0 0.0 2.5743942859448903 0.0 72-73 0.0 0.0 0.0 2.8016300481946077 0.0 74-75 0.0 0.0 0.0 3.0299868048764482 0.0 76-77 0.0 2.0381716947682984E-5 0.0 3.270939462631956 0.0 78-79 0.0 4.076343389536597E-5 0.0 3.539183239380412 0.0 80-81 0.0 4.076343389536597E-5 0.0 3.8246699486666076 0.0 82-83 0.0 4.076343389536597E-5 0.0 4.117657129789551 0.0 84-85 0.0 4.076343389536597E-5 0.0 4.4106443109124935 0.0 86-87 0.0 4.076343389536597E-5 0.0 4.710887383268812 0.0 88-89 0.0 4.076343389536597E-5 0.0 5.028332624728974 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4890 0.0 46.531673 1 GTATCAA 8185 0.0 40.335564 1 ATCAACG 10300 0.0 31.539967 3 TCAACGC 10380 0.0 31.293694 4 TATCAAC 10530 0.0 31.07848 2 CAACGCA 10585 0.0 30.642763 5 AACGCAG 10855 0.0 29.924326 6 CGCAGAG 12675 0.0 25.440163 8 ACGCAGA 12690 0.0 25.410091 7 GGACCGT 195 1.5752448E-8 24.353619 6 GTACATG 8265 0.0 24.02456 1 TACATGG 8225 0.0 23.79196 2 GCAGAGT 14070 0.0 22.546568 9 GGACCGA 875 0.0 22.252249 6 TATACCG 150 3.0401821E-5 22.161793 5 AGGACCG 1050 0.0 22.16179 5 TAAGGTG 1290 0.0 22.088165 5 ACATGGG 8570 0.0 21.890648 3 GAGTACT 8610 0.0 21.263176 12-13 GTAAGGT 1245 0.0 20.59788 4 >>END_MODULE