FastQCFastQC Report
Thu 26 May 2016
SRR937955_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937955_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731578
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116170.42528531127428904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90780.33233537537643076No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58680.21482088375290764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57190.20936616124452603No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39890.14603280594586718No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34460.12615418633478523No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32290.11821006026553149No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32280.11817345138963632No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30280.1108516762106006No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29720.10880157916047062No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29370.10752026850413936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161100.026.9388241
GGTATCA118150.025.7928851
TAAGGTG17300.024.9980285
ATCAACG185300.023.1848073
GAGTACT104700.023.18386512-13
CAACGCA186000.023.0464525
GTAAGGT16500.023.0418054
TCAACGC186650.023.0171174
TATCAAC187850.022.895382
GTACATG84900.022.6375891
AACGCAG190350.022.5897126
TACATGG84550.021.9210572
GTACTTT110900.021.67456814-15
AGAGTAC145550.021.65450510-11
ACATGGG83150.021.318523
AGTACTT108150.020.95093512-13
AGGTAAG17650.020.7327292
GGTAAGG18200.020.628433
ACGCAGA208250.020.622197
CGCAGAG209150.020.5107358
Produced by FastQC (version 0.11.5)