FastQCFastQC Report
Thu 26 May 2016
SRR937955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731578
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111500.40818896623124074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94410.34562439732638056No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67420.24681704128529372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60900.2229480542016373No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31300.11458578155190882No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31080.1137803862822149No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29540.1081426193943574No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29350.10744705075234902No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA29090.10649521997907437No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28580.10462816730842026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA404.498627E-429.68434146-47
GTATCAA169650.025.3696751
GGTATCA125700.024.2895741
GAGTACT115150.022.16980712-13
AGAGTAC153750.021.51559810-11
TATCAAC200400.021.5006052
TCAACGC202000.021.3040264
CAACGCA202350.021.2671765
ATCAACG202550.021.2469563
AGTACTT119850.021.0031912-13
GTACTTT121450.020.82425714-15
AACGCAG208000.020.7562056
TAAGGTG18200.020.3792255
GTACATG83500.020.10521
GTAAGGT17050.020.0804084
TACATGG82600.019.8060822
GTGTAGC18200.019.5979231
GTAGCCC15900.019.1409383
ACGCAGA227100.018.9059587
ACATGGG82550.018.7793643
Produced by FastQC (version 0.11.5)