##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937955_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731578 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.994108899690946 31.0 31.0 34.0 30.0 34.0 2 32.28341310407391 33.0 31.0 34.0 31.0 34.0 3 32.35427653905545 34.0 31.0 34.0 30.0 34.0 4 35.95116485782211 37.0 35.0 37.0 35.0 37.0 5 35.8567242084978 37.0 35.0 37.0 35.0 37.0 6 35.9163051540172 37.0 35.0 37.0 35.0 37.0 7 35.87606907069833 37.0 35.0 37.0 35.0 37.0 8 35.863634499911775 37.0 35.0 37.0 35.0 37.0 9 37.45567141044481 39.0 37.0 39.0 35.0 39.0 10-11 37.38648630937868 39.0 37.0 39.0 34.0 39.0 12-13 37.281340126476344 39.0 37.0 39.0 34.0 39.0 14-15 38.28979018721047 40.0 38.0 41.0 33.5 41.0 16-17 38.13332568207827 40.0 37.0 41.0 33.0 41.0 18-19 38.454631169236244 40.0 38.0 41.0 34.0 41.0 20-21 38.40915507446611 40.0 38.0 41.0 34.0 41.0 22-23 38.247219189787 40.0 38.0 41.0 33.5 41.0 24-25 38.065223654605504 40.0 37.0 41.0 33.0 41.0 26-27 37.813153605718014 40.0 37.0 41.0 33.0 41.0 28-29 37.64056087726581 39.5 37.0 41.0 32.5 41.0 30-31 37.30521222531445 39.0 36.0 40.0 32.0 41.0 32-33 36.985069436054914 39.0 36.0 40.0 31.0 41.0 34-35 36.81256164019479 39.0 35.0 40.0 31.0 41.0 36-37 36.68897190561646 39.0 35.0 40.0 30.0 41.0 38-39 36.40089721033044 38.0 35.0 40.0 30.0 41.0 40-41 36.079573052645756 38.0 35.0 40.0 30.0 41.0 42-43 35.698533045734 38.0 35.0 40.0 28.5 41.0 44-45 35.30895292025342 38.0 34.0 40.0 27.0 41.0 46-47 34.80069322567395 38.0 33.5 40.0 25.5 41.0 48-49 34.21377606643486 38.0 33.0 40.0 24.0 41.0 50-51 33.63908956654359 37.0 32.5 40.0 22.5 41.0 52-53 32.862285828923795 37.0 31.0 39.0 19.5 40.0 54-55 32.2470533515792 36.0 31.0 39.0 16.0 40.0 56-57 31.644287111698805 35.5 30.0 39.0 11.0 40.0 58-59 30.897353471143784 35.0 28.5 38.5 7.5 40.0 60-61 30.146720503679557 34.5 28.0 38.0 4.0 40.0 62-63 29.330472532726503 34.0 26.5 38.0 2.0 40.0 64-65 28.497287648384926 33.5 26.0 37.0 2.0 39.0 66-67 27.6548573754804 33.0 24.0 36.5 2.0 39.0 68-69 26.74590895811871 32.0 22.0 36.0 2.0 39.0 70-71 25.87138514807192 31.0 19.5 35.5 2.0 38.0 72-73 24.96422745387465 31.0 16.0 35.0 2.0 38.0 74-75 24.127276065336595 30.0 10.0 34.0 2.0 37.0 76-77 23.14018527019913 28.5 8.0 33.5 2.0 35.5 78-79 23.636712186142955 30.0 7.0 34.0 2.0 36.0 80-81 23.19649228394723 30.0 2.0 34.0 2.0 36.0 82-83 22.42401223761503 29.0 2.0 34.0 2.0 35.5 84-85 21.529405164340908 29.0 2.0 34.0 2.0 35.0 86-87 20.55542327548399 27.5 2.0 33.5 2.0 35.0 88-89 19.479689395653356 26.0 2.0 33.0 2.0 35.0 90-91 18.562508374280362 25.0 2.0 32.5 2.0 34.5 92-93 17.394894819038665 21.5 2.0 32.0 2.0 34.0 94-95 16.25380274698361 12.5 2.0 31.5 2.0 34.0 96-97 15.01558348324668 2.0 2.0 31.0 2.0 34.0 98-99 13.553523091780649 2.0 2.0 30.5 2.0 34.0 100-101 10.86257394077709 2.0 2.0 24.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28998666655198946 1101 2 0.29092714402166564 1101 3 0.28554745461952535 1101 4 0.12373744431262423 1101 5 0.10992484314350293 1101 6 0.15267355423993934 1101 7 0.28966263970603023 1101 8 0.21851829183235338 1101 9 0.09440386875083107 1101 10-11 0.3354579532800912 1101 12-13 0.3703566839472714 1101 14-15 0.6152295259525786 1101 16-17 0.5804026148129893 1101 18-19 0.4669218357294085 1101 20-21 0.42447429039917495 1101 22-23 0.5445258158517134 1101 24-25 0.5469649437925668 1101 26-27 0.6711363700358888 1101 28-29 0.6872659043481306 1101 30-31 0.7286754430011868 1101 32-33 0.7992180581434596 1101 34-35 0.8848095871415111 1101 36-37 1.0515355044391583 1101 38-39 1.1238952953724493 1101 40-41 1.3196276695185887 1101 42-43 1.3687844604959736 1101 44-45 1.4045747978364602 1101 46-47 1.6291189743835943 1101 48-49 1.8030834885767177 1101 50-51 1.6298591357054804 1101 52-53 1.9122245528256059 1101 54-55 2.0717152948373823 1101 56-57 2.060673669238273 1101 58-59 2.5230747156679882 1101 60-61 2.446569840585301 1101 62-63 2.543721948350079 1101 64-65 2.854144141344726 1101 66-67 3.19501627846876 1101 68-69 3.3710883102630795 1101 70-71 3.4922978477055757 1101 72-73 3.825745371791971 1101 74-75 3.945926267804964 1101 76-77 3.844849343039453 1101 78-79 4.134407248742026 1101 80-81 4.4802248126018185 1101 82-83 4.52607836551012 1101 84-85 4.735148258993387 1101 86-87 5.024781327550887 1101 88-89 5.287681595742892 1101 90-91 5.398543197519334 1101 92-93 5.600982762363291 1101 94-95 5.625367904407465 1101 96-97 5.244940600613079 1101 98-99 5.147182193184037 1101 100-101 4.212844459063826 1102 1 0.23016255590689383 1102 2 0.22018387383284477 1102 3 0.2626886908330306 1102 4 0.17666583662762037 1102 5 0.028982527592120277 1102 6 0.1205565543597018 1102 7 0.13402136460080527 1102 8 0.14953734136805963 1102 9 0.18128442548274393 1102 10-11 0.3427250721196131 1102 12-13 0.3203710214113613 1102 14-15 0.4536160565764149 1102 16-17 0.4636634017098089 1102 18-19 0.43923463382211025 1102 20-21 0.36729111285908544 1102 22-23 0.49437154535547023 1102 24-25 0.5575267470597112 1102 26-27 0.5710810110652957 1102 28-29 0.4825734483544224 1102 30-31 0.63852427766939 1102 32-33 0.6648683030378351 1102 34-35 0.7907020379496572 1102 36-37 0.7054549720300045 1102 38-39 0.7726105258645504 1102 40-41 0.9351671810385582 1102 42-43 1.0002776803724842 1102 44-45 1.1008036743842595 1102 46-47 1.0818523370606243 1102 48-49 1.3342970275875814 1102 50-51 1.5348939623163105 1102 52-53 1.7528252107747662 1102 54-55 1.9958326842521643 1102 56-57 1.9865594745006412 1102 58-59 2.2491167977455646 1102 60-61 2.205398107088673 1102 62-63 2.5782629673872677 1102 64-65 2.823492062983462 1102 66-67 2.825051907607179 1102 68-69 3.1565435934256776 1102 70-71 3.4546138528219963 1102 72-73 3.6292934851730685 1102 74-75 3.674406258940124 1102 76-77 4.020489219894678 1102 78-79 4.004496242878634 1102 80-81 4.242443912511568 1102 82-83 4.323161193148181 1102 84-85 4.448300513293393 1102 86-87 4.4710484013253975 1102 88-89 4.5828019347552384 1102 90-91 4.699476614274378 1102 92-93 4.743602950130054 1102 94-95 4.735435566743362 1102 96-97 4.712417243973521 1102 98-99 4.761032384123872 1102 100-101 3.7363520355416195 1103 1 0.2709680591633443 1103 2 0.23528587873229156 1103 3 0.2441994169387698 1103 4 0.151168450532694 1103 5 0.043176516437256396 1103 6 0.1034967782602223 1103 7 0.20283864637575988 1103 8 0.15627467284618746 1103 9 0.2736023439024464 1103 10-11 0.28981578868158664 1103 12-13 0.34708869249634944 1103 14-15 0.5400216831458735 1103 16-17 0.5005920455265596 1103 18-19 0.45484601358130305 1103 20-21 0.37673447982395203 1103 22-23 0.4686671611291402 1103 24-25 0.606053634965356 1103 26-27 0.6677091266677024 1103 28-29 0.7227959610105472 1103 30-31 0.6817632639514031 1103 32-33 0.7611053073348941 1103 34-35 0.8791379293586061 1103 36-37 0.9702296482152448 1103 38-39 1.0652159679369646 1103 40-41 1.140653576448102 1103 42-43 1.2428535048805927 1103 44-45 1.290371293380133 1103 46-47 1.290477367475468 1103 48-49 1.5231156440723126 1103 50-51 1.8532409951265478 1103 52-53 1.9741769351489253 1103 54-55 2.030745827718107 1103 56-57 2.0724111374545267 1103 58-59 2.4844633380124606 1103 60-61 2.7010600050223452 1103 62-63 2.6047937200454676 1103 64-65 2.691986238687967 1103 66-67 2.9854735400635413 1103 68-69 3.205515778310147 1103 70-71 3.282121660283618 1103 72-73 3.594390676849205 1103 74-75 3.587738562696444 1103 76-77 3.7205316530351666 1103 78-79 3.9766256128719952 1103 80-81 4.210256736592093 1103 82-83 4.307087241958399 1103 84-85 4.483349047120608 1103 86-87 4.496158662526916 1103 88-89 4.738479146416584 1103 90-91 5.018302607097656 1103 92-93 4.923969044485769 1103 94-95 4.714599182082367 1103 96-97 4.605544197315373 1103 98-99 4.717968061743033 1103 100-101 3.7703339827060116 1104 1 0.27848645114402615 1104 2 0.3112446484308862 1104 3 0.25065511817654595 1104 4 0.21117387586313185 1104 5 0.11627649058130629 1104 6 0.19849668986379498 1104 7 0.19217941900468816 1104 8 0.2767620354726219 1104 9 0.2678452506420115 1104 10-11 0.38239667693304114 1104 12-13 0.45631752763038946 1104 14-15 0.5514267553326064 1104 16-17 0.510596863131596 1104 18-19 0.48552343956586697 1104 20-21 0.5058794433272809 1104 22-23 0.49703233006574976 1104 24-25 0.6237682780537455 1104 26-27 0.6498048456289567 1104 28-29 0.7039052311433807 1104 30-31 0.6792892690739762 1104 32-33 0.6846187833695225 1104 34-35 0.7133623170476397 1104 36-37 0.8803525855399741 1104 38-39 1.0115565770325077 1104 40-41 1.1140654518445032 1104 42-43 1.1569037295179996 1104 44-45 1.3451534569709764 1104 46-47 1.3944155385976913 1104 48-49 1.5972596628388729 1104 50-51 1.802798957765802 1104 52-53 1.8551168985086122 1104 54-55 1.9321002542794687 1104 56-57 2.070250949877316 1104 58-59 2.4351947631616895 1104 60-61 2.5656658287996557 1104 62-63 2.652948223732704 1104 64-65 2.9485679048279394 1104 66-67 3.1156209742468803 1104 68-69 3.3021472945076873 1104 70-71 3.4813417828452558 1104 72-73 3.773178104447009 1104 74-75 3.6167980579086745 1104 76-77 3.7683782885568284 1104 78-79 4.009274279080476 1104 80-81 4.331697123149652 1104 82-83 4.498412735265685 1104 84-85 4.53045619131948 1104 86-87 4.64597761560389 1104 88-89 4.474076330548545 1104 90-91 4.803139775362457 1104 92-93 4.900658769727588 1104 94-95 5.017687311105785 1104 96-97 4.970424878719198 1104 98-99 4.824252881646373 1104 100-101 3.779133155978469 1105 1 0.26346480940280514 1105 2 0.19545033717771076 1105 3 0.14344662723756585 1105 4 0.07594068133228404 1105 5 0.03968014720889812 1105 6 0.10717943168799593 1105 7 0.16731936963778082 1105 8 0.1338673254051841 1105 9 0.1259563198309337 1105 10-11 0.13295918203348123 1105 12-13 0.26837292868619755 1105 14-15 0.38885920437859767 1105 16-17 0.39836954430168703 1105 18-19 0.42057993383695447 1105 20-21 0.34380166659755673 1105 22-23 0.38910719491084933 1105 24-25 0.45819756456517524 1105 26-27 0.4010907513655013 1105 28-29 0.5509329229276787 1105 30-31 0.7292454450779289 1105 32-33 0.7013833828012324 1105 34-35 0.7351183874924629 1105 36-37 0.7376289056384948 1105 38-39 0.8185479086778855 1105 40-41 0.8806312005565431 1105 42-43 0.8853010695551191 1105 44-45 1.1041508227619659 1105 46-47 1.1812894304122992 1105 48-49 1.426315674357113 1105 50-51 1.504353702786453 1105 52-53 1.6452744105350448 1105 54-55 1.838492635899037 1105 56-57 1.759158868613742 1105 58-59 2.2293781742689234 1105 60-61 2.3455517936747547 1105 62-63 2.5612193538406878 1105 64-65 2.823213788922743 1105 66-67 3.027673447520602 1105 68-69 3.4785601414564873 1105 70-71 3.658399470485616 1105 72-73 3.8590163695254844 1105 74-75 3.729041936591983 1105 76-77 3.6636371495356634 1105 78-79 3.898967638287804 1105 80-81 4.016677972010385 1105 82-83 4.154012694780153 1105 84-85 4.284296686281575 1105 86-87 4.486922343317502 1105 88-89 4.381258087260189 1105 90-91 4.363554293664141 1105 92-93 4.630226096527419 1105 94-95 4.814896406148215 1105 96-97 4.7279988919903975 1105 98-99 4.335901347488559 1105 100-101 3.4825624101650643 1106 1 0.17682830839848407 1106 2 0.09517993988274753 1106 3 0.07819738992068892 1106 4 0.16180772858785986 1106 5 0.06236996210590462 1106 6 0.12273048328558644 1106 7 0.11137614283279618 1106 8 0.14968064955034066 1106 9 0.1826018772721838 1106 10-11 0.20752188078689215 1106 12-13 0.24730864638452488 1106 14-15 0.14639885981933531 1106 16-17 0.05454228550205187 1106 18-19 -0.03928206467208639 1106 20-21 0.04253791202868484 1106 22-23 0.01931897092232049 1106 24-25 0.09277486972252547 1106 26-27 -0.07122562966727486 1106 28-29 0.169289046803776 1106 30-31 0.3015043179305579 1106 32-33 0.4169155025711575 1106 34-35 0.2635470341337225 1106 36-37 0.4280178477924963 1106 38-39 0.45046167025063255 1106 40-41 0.5577391610855642 1106 42-43 0.5810930983097222 1106 44-45 0.6532722421229451 1106 46-47 0.86660098855873 1106 48-49 1.0407682912060778 1106 50-51 1.12934114959819 1106 52-53 1.186260456832109 1106 54-55 1.4439439526588416 1106 56-57 1.5792257766212607 1106 58-59 1.9725699404659025 1106 60-61 1.958524332935621 1106 62-63 1.9534406273838307 1106 64-65 2.097158566297537 1106 66-67 2.3393672007820037 1106 68-69 2.4697994738380906 1106 70-71 2.5267908080794967 1106 72-73 2.88080222528621 1106 74-75 2.9573374254912075 1106 76-77 2.943264585161945 1106 78-79 3.247551696963253 1106 80-81 3.3136579632325507 1106 82-83 3.5648389919569787 1106 84-85 3.7611470521478942 1106 86-87 3.903712101419025 1106 88-89 3.8380243237644542 1106 90-91 3.7272113137374525 1106 92-93 3.7088565466034353 1106 94-95 3.9191227261054316 1106 96-97 4.032032705971 1106 98-99 3.704975772895928 1106 100-101 3.2146740035057793 1107 1 0.21462467458822942 1107 2 0.2282147505072416 1107 3 0.19619799792720727 1107 4 0.1486102820093933 1107 5 0.05657087159411134 1107 6 0.10912739884253853 1107 7 0.14222700588428694 1107 8 0.1504062434470157 1107 9 0.14503335271205486 1107 10-11 0.27379336894781403 1107 12-13 0.26509528138236504 1107 14-15 0.4176219988543153 1107 16-17 0.4129553730546007 1107 18-19 0.30619982037454463 1107 20-21 0.33601401239375406 1107 22-23 0.4436332879062448 1107 24-25 0.3982393508077493 1107 26-27 0.398451749930139 1107 28-29 0.5288646284078098 1107 30-31 0.615007260123754 1107 32-33 0.7483935540633624 1107 34-35 0.7582248543621759 1107 36-37 0.8543705175599214 1107 38-39 0.8412857763839128 1107 40-41 0.9229230431750111 1107 42-43 1.011687855018991 1107 44-45 0.9883696434547176 1107 46-47 1.056353505719862 1107 48-49 1.2359703530248751 1107 50-51 1.2721522444434754 1107 52-53 1.4309873254068535 1107 54-55 1.3794959051175155 1107 56-57 1.5149786182412193 1107 58-59 1.7055049433341907 1107 60-61 1.8513026538367257 1107 62-63 2.072409337176243 1107 64-65 2.180092147567848 1107 66-67 2.48576258796032 1107 68-69 2.6423617195666402 1107 70-71 2.8469513315774826 1107 72-73 3.019195366725686 1107 74-75 2.897834094513481 1107 76-77 2.717811047164819 1107 78-79 2.756080512882786 1107 80-81 2.9324320234565846 1107 82-83 3.224859334316367 1107 84-85 3.2409087717436726 1107 86-87 3.629916320335848 1107 88-89 3.833608621758149 1107 90-91 4.06329315558294 1107 92-93 3.993650542905737 1107 94-95 3.7134798312585247 1107 96-97 3.9149220440248076 1107 98-99 3.5774444118657485 1107 100-101 2.8157356605898443 1108 1 0.2573099620663726 1108 2 0.2610020980927956 1108 3 0.18440278421199707 1108 4 0.14872633147506775 1108 5 -0.02508180234075752 1108 6 0.14481895177735282 1108 7 0.20794932677104327 1108 8 0.17015637815210738 1108 9 0.22482599660630598 1108 10-11 0.23644682918528304 1108 12-13 0.22855643240684032 1108 14-15 0.420346867296729 1108 16-17 0.40366709768055387 1108 18-19 0.4339300021282497 1108 20-21 0.4256379352084494 1108 22-23 0.4808308525407412 1108 24-25 0.5096301051468188 1108 26-27 0.561119500444839 1108 28-29 0.47425876680326695 1108 30-31 0.4523600881480263 1108 32-33 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3.966754949254902 2311 92-93 3.9495910027666064 2311 94-95 3.5709660484701438 2311 96-97 3.806759430390395 2311 98-99 3.5078041039730863 2311 100-101 3.123910461328265 2312 1 -0.1605791572157571 2312 2 -0.1316905844427083 2312 3 -0.16273779761873897 2312 4 -0.09332110958195727 2312 5 -0.1421206460877471 2312 6 -0.12297615977014686 2312 7 -0.11835200419942993 2312 8 -0.09312953072156205 2312 9 -0.21423821872993898 2312 10-11 -0.1087811576459643 2312 12-13 -0.03142974399876408 2312 14-15 -0.09876588024764743 2312 16-17 -0.13239344699722722 2312 18-19 -0.1276001442844148 2312 20-21 -0.07805956823330717 2312 22-23 -0.1404818130478489 2312 24-25 -0.09588457101153125 2312 26-27 -0.18877044769971718 2312 28-29 -0.16031701771462536 2312 30-31 -0.1933143678387168 2312 32-33 -0.11777599872848299 2312 34-35 0.03690859125639179 2312 36-37 -0.004190094307979564 2312 38-39 0.11398384907346326 2312 40-41 0.12008773423419683 2312 42-43 0.43855672859343287 2312 44-45 0.5805332890380441 2312 46-47 0.6864382342506232 2312 48-49 0.6322618468101382 2312 50-51 0.8388140166276301 2312 52-53 0.7897602714426668 2312 54-55 0.9072630961776014 2312 56-57 0.9433982510182766 2312 58-59 1.4298834970074097 2312 60-61 1.5319140970648668 2312 62-63 1.7183536286081313 2312 64-65 1.8702361353258823 2312 66-67 2.277548962512757 2312 68-69 2.6419730400728305 2312 70-71 2.970112346736343 2312 72-73 2.8179903883171917 2312 74-75 3.1503879527152847 2312 76-77 3.3037594197313886 2312 78-79 3.4440576124628315 2312 80-81 3.334602834418355 2312 82-83 3.377896126789228 2312 84-85 3.5118367332384928 2312 86-87 3.6820314025757526 2312 88-89 3.6591419134164056 2312 90-91 3.674998202550178 2312 92-93 3.6290144746723314 2312 94-95 3.29050405066954 2312 96-97 3.3334800143117054 2312 98-99 3.2815121227126767 2312 100-101 3.174873713427944 2313 1 -0.24927334874323037 2313 2 -0.28179584552625414 2313 3 -0.29235448872801584 2313 4 -0.16288870945682277 2313 5 -0.14829198439380065 2313 6 -0.17488775523974454 2313 7 -0.2617529594653192 2313 8 -0.18896291164128343 2313 9 -0.29184802963172274 2313 10-11 -0.2923327053455509 2313 12-13 -0.21485751111345763 2313 14-15 -0.272067488577882 2313 16-17 -0.2435593850883464 2313 18-19 -0.24725739690855164 2313 20-21 -0.18265041153794215 2313 22-23 -0.16606419762063496 2313 24-25 -0.22904583197684047 2313 26-27 -0.19759850588632588 2313 28-29 -0.093021359809903 2313 30-31 -0.06032452982724834 2313 32-33 0.018995557288079112 2313 34-35 0.08305210104451533 2313 36-37 0.12774265414949326 2313 38-39 0.04972884919998677 2313 40-41 0.23562036696157662 2313 42-43 0.22646908141434352 2313 44-45 0.33603477899574585 2313 46-47 0.39188024682552935 2313 48-49 0.5340628627178745 2313 50-51 0.7217120968920838 2313 52-53 0.90233627809625 2313 54-55 1.065928476108411 2313 56-57 1.261525774916155 2313 58-59 1.4025932090862625 2313 60-61 1.3131598772722022 2313 62-63 1.6815951056626481 2313 64-65 1.8296149415368816 2313 66-67 2.0801948998783253 2313 68-69 2.364993239317581 2313 70-71 2.698071172980651 2313 72-73 2.9038712167815994 2313 74-75 3.3469304155581554 2313 76-77 3.2772038096101106 2313 78-79 3.2877974163800516 2313 80-81 3.370156414112561 2313 82-83 3.546183809663706 2313 84-85 3.64369983762197 2313 86-87 3.7376976757869897 2313 88-89 3.765417594668687 2313 90-91 3.9524225721089543 2313 92-93 3.8800703885626966 2313 94-95 3.950849006053666 2313 96-97 3.9298593510269146 2313 98-99 3.703919330941517 2313 100-101 3.2883024121161046 2314 1 -0.20009775683101338 2314 2 -0.23986382802052475 2314 3 -0.23110172388103933 2314 4 -0.17660074364242462 2314 5 -0.05672885893968527 2314 6 -0.18426980079838984 2314 7 -0.23289994202099962 2314 8 -0.15949556935669307 2314 9 -0.23200814588460617 2314 10-11 -0.29238839297887154 2314 12-13 -0.20703301344122593 2314 14-15 -0.20626589150938912 2314 16-17 -0.10745374674849018 2314 18-19 -0.18961651434980809 2314 20-21 -0.21742667821700223 2314 22-23 -0.2176603856810786 2314 24-25 -0.14033312095537553 2314 26-27 -0.15940795997363466 2314 28-29 -0.016848821472926545 2314 30-31 0.009998275755336294 2314 32-33 0.11825469165572855 2314 34-35 0.13695492370317197 2314 36-37 0.1718229376677769 2314 38-39 0.29903165323740666 2314 40-41 0.4629413031565406 2314 42-43 0.6821172381426734 2314 44-45 0.7413793390733474 2314 46-47 0.8520713478536734 2314 48-49 0.9702541345507854 2314 50-51 0.9228527469054342 2314 52-53 1.1350509907909796 2314 54-55 1.4949863382007464 2314 56-57 1.782714919393431 2314 58-59 1.922746959780433 2314 60-61 2.0807741178106376 2314 62-63 2.303800186267747 2314 64-65 2.240189076634195 2314 66-67 2.505321556227738 2314 68-69 2.9279873432518393 2314 70-71 3.2926478138013557 2314 72-73 3.5301239409083642 2314 74-75 3.644535748276354 2314 76-77 3.5822899944767634 2314 78-79 3.7331692237783187 2314 80-81 3.8399610055469218 2314 82-83 4.085036113954143 2314 84-85 4.398780673834519 2314 86-87 4.574182991530371 2314 88-89 4.764062217103234 2314 90-91 4.972090761858073 2314 92-93 5.113375049001302 2314 94-95 5.179752692641024 2314 96-97 5.02536221145632 2314 98-99 4.6269599021337555 2314 100-101 3.8230629409857393 2315 1 -0.3270813438426394 2315 2 -0.3558303403939824 2315 3 -0.3201389793885028 2315 4 -0.28590892327429174 2315 5 -0.20433610317928697 2315 6 -0.2054584475043697 2315 7 -0.31313736530788105 2315 8 -0.2460240602061674 2315 9 -0.40334759039602375 2315 10-11 -0.27672149456963524 2315 12-13 -0.23962350450977965 2315 14-15 -0.3439164072054055 2315 16-17 -0.26553575499324467 2315 18-19 -0.3317729642518543 2315 20-21 -0.29312486462499265 2315 22-23 -0.21745561552732084 2315 24-25 -0.16326930477732304 2315 26-27 -0.20157872176815061 2315 28-29 -0.055816581733317605 2315 30-31 -0.12573340048103177 2315 32-33 -0.09238911781775272 2315 34-35 -0.059526442488063935 2315 36-37 0.024165491713063147 2315 38-39 0.08706977351344847 2315 40-41 0.15515524833428884 2315 42-43 0.14001319178535 2315 44-45 0.3159815097470684 2315 46-47 0.24789211011607648 2315 48-49 0.2912680110314483 2315 50-51 0.520321688722639 2315 52-53 0.6452987461633128 2315 54-55 0.8693836286292225 2315 56-57 1.083771632469162 2315 58-59 1.5209432760094046 2315 60-61 1.7402758657881243 2315 62-63 1.5042118733591359 2315 64-65 2.233065662433166 2315 66-67 2.522487295098454 2315 68-69 2.8946785603186562 2315 70-71 3.0733023186621686 2315 72-73 3.250730148636677 2315 74-75 3.729500499736581 2315 76-77 3.805985864560011 2315 78-79 3.72629830438785 2315 80-81 3.950078742880759 2315 82-83 4.17347683877443 2315 84-85 4.281281247686358 2315 86-87 4.309489824743427 2315 88-89 4.736196191829531 2315 90-91 5.045740916454033 2315 92-93 5.163414324136973 2315 94-95 5.143551971651917 2315 96-97 5.285287866679527 2315 98-99 5.2448854924029895 2315 100-101 4.644635091364096 2316 1 -0.28331706791755806 2316 2 -0.3766502777020193 2316 3 -0.2825795412601835 2316 4 -0.18803073875535858 2316 5 -0.22126293286804355 2316 6 -0.2675119971278477 2316 7 -0.28993616778222986 2316 8 -0.2672663494109102 2316 9 -0.3675459406377968 2316 10-11 -0.32953272051066307 2316 12-13 -0.38373600526397666 2316 14-15 -0.4016731727368992 2316 16-17 -0.30155662549785234 2316 18-19 -0.33364050665224454 2316 20-21 -0.3201815316077088 2316 22-23 -0.29750164326463135 2316 24-25 -0.22189375923945676 2316 26-27 -0.33740881563370095 2316 28-29 -0.34962697158997713 2316 30-31 -0.1438336214256637 2316 32-33 -0.05864119007152624 2316 34-35 -0.06888053140032468 2316 36-37 -0.0911684679652609 2316 38-39 -0.11001427451399337 2316 40-41 -0.029002245826056594 2316 42-43 0.01668212823431503 2316 44-45 0.13586681391226563 2316 46-47 0.15907552863807695 2316 48-49 0.41185809696713704 2316 50-51 0.5705367434128945 2316 52-53 0.7264359932908846 2316 54-55 1.0843736731916565 2316 56-57 1.1722207390656116 2316 58-59 1.4749847814209645 2316 60-61 1.5820542258560444 2316 62-63 1.8211438747037612 2316 64-65 2.2022833920187743 2316 66-67 2.595885733833004 2316 68-69 3.001651389013304 2316 70-71 3.2759829602922785 2316 72-73 3.381589131946594 2316 74-75 3.6088483539303624 2316 76-77 3.9655142095439366 2316 78-79 4.17053693608263 2316 80-81 4.203377253731183 2316 82-83 4.542529650000372 2316 84-85 4.973996970382171 2316 86-87 4.941220519383005 2316 88-89 5.1707218791569645 2316 90-91 5.420663844415742 2316 92-93 5.398079646173112 2316 94-95 5.592874053760575 2316 96-97 5.492549733474911 2316 98-99 5.3984865451222745 2316 100-101 4.6813955086592625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 22.0 4 34.0 5 100.0 6 193.0 7 450.0 8 1189.0 9 2435.0 10 3892.0 11 6513.0 12 10144.0 13 14895.0 14 20377.0 15 28280.0 16 36673.0 17 48033.0 18 51533.0 19 49553.0 20 52606.0 21 57324.0 22 63624.0 23 69467.0 24 74571.0 25 79237.0 26 85809.0 27 96130.0 28 110019.0 29 124837.0 30 138226.0 31 153023.0 32 170247.0 33 191926.0 34 220920.0 35 253120.0 36 265595.0 37 199798.0 38 50020.0 39 760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4146775233949 19.649081959219178 15.588425444925974 22.347815072459948 2 16.086818506177185 20.869005195768548 39.36829911165575 23.675877186398516 3 20.165249802214973 23.965248411038473 30.328379890763358 25.5411218959832 4 13.415348028568468 17.668804774444336 37.10642666068226 31.809420536304938 5 13.624171428801791 37.46653556364831 34.469881182697776 14.439411824852133 6 31.86236375544237 37.022313484115585 16.879992766083475 14.235329994358569 7 28.25886721887495 33.04298833860867 20.340806669258573 18.3573377732578 8 26.2063653433388 34.969081917268916 19.31191295271992 19.512639786672366 9 25.63807440241501 17.246368216466816 20.29522861876908 36.82032876234909 10-11 24.83516853628196 26.746884035528183 27.14485912538467 21.273088302805192 12-13 26.30280006648172 23.90751792553608 27.493723408227773 22.295958599754428 14-15 22.517135245110197 25.55027269041424 26.291419518574887 25.641172545900677 16-17 21.80424648316834 28.719370268760404 27.13977049163524 22.336612756436022 18-19 21.787864011205244 27.16999111868671 29.561685589794617 21.48045928031343 20-21 22.282523142300896 26.055397283182103 29.992187665883968 21.669891908633033 22-23 22.042274635767676 25.998209086579244 30.039332734653772 21.92018354299931 24-25 22.185753460063047 26.204096082039523 29.938040933729663 21.67210952416777 26-27 21.68800543894314 26.387276475582418 30.039250746774655 21.885467338699787 28-29 21.696744519102147 27.09578492724718 29.65750932245025 21.549961231200427 30-31 21.89494497319864 26.380465796693343 29.530330087590396 22.194259142517623 32-33 21.67659133292185 26.412352127598037 29.625604687107597 22.28545185237251 34-35 22.57221600317065 26.514327871043207 29.32730812496968 21.58614800081646 36-37 22.470369032781456 25.982904637442456 29.599044062558406 21.947682267217683 38-39 21.893556764581675 26.640665343901542 29.428137480438142 22.037640411078645 40-41 22.49080067491312 26.447814779294 29.226788053883944 21.834596491908933 42-43 22.08701417591564 26.524747032550916 28.966491343265986 22.421747448267453 44-45 22.24441003492796 26.535033486431068 29.185208968253285 22.035347510387684 46-47 22.78666814322493 26.990868829458435 28.430201962053065 21.79226106526357 48-49 22.470949026533383 27.017112452948446 28.333183236942162 22.178755283576013 50-51 22.360390221329943 27.235594224290864 28.485146680783046 21.918868873596143 52-53 22.90809561359771 27.047937126452183 28.043973117370253 21.999994142579858 54-55 22.3519518754361 27.09887837726032 28.304445269364447 22.244724477939126 56-57 22.583255020817646 27.144319929476634 28.09067802225601 22.18174702744971 58-59 22.757999222427475 26.894637458641125 27.946154200978334 22.401209117953066 60-61 22.23344894416341 27.212658763542542 28.36380656162848 22.190085730665572 62-63 22.259935465873575 27.15785527632746 28.404460718310077 22.177748539488896 64-65 21.974770627088077 27.450872718992464 28.075273706260628 22.499082947658824 66-67 22.098319413715853 27.183917419116767 27.967988449161137 22.74977471800625 68-69 22.562313407564556 26.90152678602481 28.062733497952628 22.473426308458002 70-71 22.629099370400553 26.99046119129675 27.61934676586208 22.76109267244062 72-73 22.543322975454462 26.9634818873793 27.82618898394187 22.667006153224364 74-75 22.40049524487311 27.651086661263196 27.393579828216513 22.55483826564718 76-77 22.448507785609635 26.97869143769645 27.942108920191917 22.63069185650199 78-79 22.53312920224134 27.27657786085552 27.826406567925204 22.36388636897793 80-81 22.799951383377216 27.16829039353106 27.564490242995255 22.467267980096473 82-83 22.666142904369327 27.241381077708045 27.793290107079617 22.299185910843015 84-85 22.56731124423521 27.461061932971205 27.624454823128936 22.347171999664646 86-87 22.81195348031788 27.325444107591135 27.300091635002012 22.562510777088978 88-89 22.55335560617343 27.616454664666357 26.829235701854387 23.000954027305827 90-91 22.643651797659867 27.197983386109403 27.27735341941882 22.88101139681191 92-93 22.78834798054458 27.078542146700553 27.336030675309292 22.79707919744558 94-95 22.52335463237733 26.953559444394415 27.37582452340735 23.14726139982091 96-97 22.543890413446306 26.700154050262558 27.54587461597261 23.210080920318525 98-99 22.445304508968807 27.014440005008094 26.863959220640965 23.676296265382135 100-101 22.955742065575283 26.19187517251933 27.169716552117496 23.682666209787897 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 728.0 1 497.5 2 371.0 3 474.5 4 386.5 5 350.5 6 689.0 7 1655.5 8 2769.0 9 3221.5 10 2774.5 11 2206.5 12 2234.5 13 2509.5 14 2788.0 15 3146.0 16 3448.5 17 3690.0 18 4146.0 19 4648.0 20 5317.5 21 6237.5 22 7681.0 23 9678.0 24 11932.0 25 14815.0 26 18856.0 27 23679.0 28 27548.5 29 31070.0 30 35138.5 31 41278.0 32 49200.0 33 52664.0 34 53354.5 35 55977.0 36 60438.5 37 65030.5 38 68698.0 39 72308.5 40 76359.5 41 80893.0 42 84540.5 43 87839.0 44 90916.5 45 94440.0 46 96955.5 47 102434.0 48 109114.0 49 106383.5 50 99082.5 51 93954.0 52 90749.5 53 89694.0 54 89689.5 55 85048.0 56 77189.0 57 70552.5 58 65468.0 59 58540.0 60 48423.0 61 40343.5 62 34251.5 63 26965.0 64 19744.5 65 14629.5 66 10932.5 67 8343.0 68 6398.5 69 4890.0 70 3759.5 71 2743.5 72 1976.5 73 1517.5 74 1134.0 75 802.0 76 568.5 77 393.5 78 248.5 79 156.0 80 98.0 81 58.0 82 33.5 83 21.0 84 14.0 85 8.5 86 5.0 87 2.0 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011605013658771597 3 0.0028188834439287474 4 0.0012813106563312488 5 0.10744705075234902 6 3.6608875895178537E-5 7 0.0 8 1.8304437947589268E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.8304437947589268E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.026976348469639E-4 24-25 6.040464522704458E-4 26-27 0.006040464522704459 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010085745309121687 36-37 6.772642040608029E-4 38-39 0.006534684347289368 40-41 0.009646438798379545 42-43 0.005473026946329192 44-45 0.003605974275675086 46-47 1.8304437947589268E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.6608875895178537E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.491331384276781E-5 68-69 8.420041455891063E-4 70-71 0.0 72-73 7.321775179035707E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.321775179035707E-5 82-83 0.002159923677815534 84-85 0.004576109486897317 86-87 0.0032581899546708895 88-89 0.0 90-91 0.00250770799881973 92-93 0.0 94-95 0.0 96-97 7.321775179035707E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731578.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.54651895832132 #Duplication Level Percentage of deduplicated Percentage of total 1 74.55976417668755 39.17856061838279 2 14.170486359786654 14.892194603063263 3 4.8305222416147355 7.614813856428045 4 2.2447908199028825 4.7182377350197 5 1.1602753579869574 3.0484215547667355 6 0.7035033806832678 2.2179992238189885 7 0.4595375350482595 1.690296845823153 8 0.30797421278330434 1.2946378248553592 9 0.22524075376822622 1.0652055784261814 >10 1.2011618609276902 11.352518808583868 >50 0.08764545245541697 3.1461151259748648 >100 0.04239730740917311 4.313515731859482 >500 0.003140878568418915 1.2315084833159688 >1k 0.00328047317144488 3.010150314472359 >5k 2.093919045603115E-4 0.8168857123357319 >10k+ 6.979730152010383E-5 0.4089379828735873 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11150 0.40818896623124074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9441 0.34562439732638056 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6742 0.24681704128529372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6090 0.2229480542016373 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3130 0.11458578155190882 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3108 0.1137803862822149 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2954 0.1081426193943574 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2935 0.10744705075234902 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2909 0.10649521997907437 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2858 0.10462816730842026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.6608875895178537E-5 14-15 0.0 0.0 0.0 1.8304437947589268E-5 3.6608875895178537E-5 16-17 0.0 0.0 0.0 3.6608875895178537E-5 3.6608875895178537E-5 18-19 0.0 0.0 0.0 5.491331384276781E-5 3.6608875895178537E-5 20-21 0.0 0.0 0.0 9.152218973794635E-5 3.6608875895178537E-5 22-23 0.0 0.0 0.0 1.4643550358071415E-4 3.6608875895178537E-5 24-25 0.0 0.0 0.0 2.0134881742348194E-4 3.6608875895178537E-5 26-27 0.0 0.0 0.0 4.7591538663732094E-4 3.6608875895178537E-5 28-29 0.0 0.0 0.0 0.002306359181396248 3.6608875895178537E-5 30-31 0.0 0.0 0.0 0.007266861865192939 3.6608875895178537E-5 32-33 0.0 0.0 0.0 0.018121393568113375 3.6608875895178537E-5 34-35 0.0 0.0 0.0 0.034485561093258184 3.6608875895178537E-5 36-37 0.0 0.0 0.0 0.06232661121154146 3.6608875895178537E-5 38-39 0.0 0.0 0.0 0.10450003624278714 7.321775179035707E-5 40-41 0.0 0.0 0.0 0.14830255625136823 7.321775179035707E-5 42-43 0.0 0.0 0.0 0.19646153249147563 7.321775179035707E-5 44-45 0.0 0.0 0.0 0.25476116735454746 7.321775179035707E-5 46-47 0.0 0.0 0.0 0.3297544496258207 7.321775179035707E-5 48-49 0.0 0.0 0.0 0.4039423366274 7.321775179035707E-5 50-51 0.0 0.0 0.0 0.48363985945120364 7.321775179035707E-5 52-53 0.0 0.0 0.0 0.562367247063785 7.321775179035707E-5 54-55 0.0 0.0 0.0 0.6464029216811674 1.0982662768553562E-4 56-57 0.0 0.0 0.0 0.7502439981578413 1.0982662768553562E-4 58-59 0.0 0.0 0.0 0.8572700468373958 1.0982662768553562E-4 60-61 0.0 0.0 0.0 0.9686159428725813 1.2813106563312488E-4 62-63 0.0 0.0 0.0 1.080401145418509 1.4643550358071415E-4 64-65 0.0 0.0 0.0 1.1968173707651768 1.4643550358071415E-4 66-67 0.0 0.0 0.0 1.3307143343517924 1.4643550358071415E-4 68-69 1.8304437947589268E-5 0.0 0.0 1.4776806666329865 1.4643550358071415E-4 70-71 3.6608875895178537E-5 0.0 0.0 1.626331007205359 1.4643550358071415E-4 72-73 3.6608875895178537E-5 0.0 0.0 1.783254953730042 1.4643550358071415E-4 74-75 3.6608875895178537E-5 0.0 0.0 1.948763681652144 1.4643550358071415E-4 76-77 3.6608875895178537E-5 0.0 0.0 2.1208620072353783 1.4643550358071415E-4 78-79 3.6608875895178537E-5 0.0 0.0 2.3136077388234932 1.4643550358071415E-4 80-81 3.6608875895178537E-5 0.0 0.0 2.518196441763698 1.4643550358071415E-4 82-83 3.6608875895178537E-5 0.0 0.0 2.723315973404384 1.4643550358071415E-4 84-85 3.6608875895178537E-5 0.0 0.0 2.9293141180665536 1.8304437947589268E-4 86-87 3.6608875895178537E-5 0.0 0.0 3.141187987309899 1.8304437947589268E-4 88-89 3.6608875895178537E-5 0.0 0.0 3.368913499815857 1.8304437947589268E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTCGA 40 4.498627E-4 29.684341 46-47 GTATCAA 16965 0.0 25.369675 1 GGTATCA 12570 0.0 24.289574 1 GAGTACT 11515 0.0 22.169807 12-13 AGAGTAC 15375 0.0 21.515598 10-11 TATCAAC 20040 0.0 21.500605 2 TCAACGC 20200 0.0 21.304026 4 CAACGCA 20235 0.0 21.267176 5 ATCAACG 20255 0.0 21.246956 3 AGTACTT 11985 0.0 21.00319 12-13 GTACTTT 12145 0.0 20.824257 14-15 AACGCAG 20800 0.0 20.756205 6 TAAGGTG 1820 0.0 20.379225 5 GTACATG 8350 0.0 20.1052 1 GTAAGGT 1705 0.0 20.080408 4 TACATGG 8260 0.0 19.806082 2 GTGTAGC 1820 0.0 19.597923 1 GTAGCCC 1590 0.0 19.140938 3 ACGCAGA 22710 0.0 18.905958 7 ACATGGG 8255 0.0 18.779364 3 >>END_MODULE