FastQCFastQC Report
Thu 26 May 2016
SRR937954_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937954_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744666
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117480.4280302229852376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91950.33501344061536087No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61500.22407097985692975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58660.21372363704727643No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39160.14267674099507918No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35200.12824875595063295No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32530.11852079633733212No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31860.11607969785758995No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30840.11236339867947502No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC30270.11028664325641079No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28580.1041292455985537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119750.026.464131
GTATCAA163400.025.6744941
TAAGGTG17800.025.0993865
GGTAAGG17100.023.9032843
GTAAGGT17500.023.628524
AGGTAAG17450.023.4234242
GAGTACT108200.022.36940412-13
TATCAAC189200.022.0054552
CAACGCA189700.021.9729085
ATCAACG189600.021.934363
TCAACGC190850.021.8156034
AACGCAG194500.021.4531256
GTACTTT113450.021.23091314-15
AAGGTAA18350.021.0675491
AGAGTAC146950.020.86662710-11
GTACATG85950.020.6012271
TACATGG84200.020.5464442
ACATGGG82200.019.8325043
GGACCGA7000.019.6886356
AGTACTT112150.019.65423812-13
Produced by FastQC (version 0.11.5)