##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937954_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2744666 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32925499860457 33.0 31.0 34.0 31.0 34.0 2 32.54414562646238 34.0 31.0 34.0 31.0 34.0 3 32.63671973201839 34.0 31.0 34.0 31.0 34.0 4 36.131277904123856 37.0 37.0 37.0 35.0 37.0 5 36.13920783075245 37.0 37.0 37.0 35.0 37.0 6 36.182673228728014 37.0 37.0 37.0 35.0 37.0 7 36.1488294021932 37.0 37.0 37.0 35.0 37.0 8 36.12562439291338 37.0 37.0 37.0 35.0 37.0 9 37.84890620570955 39.0 38.0 39.0 35.0 39.0 10-11 37.77577198828564 39.0 38.0 39.0 35.0 39.0 12-13 37.70146240016089 39.0 38.0 39.0 35.0 39.0 14-15 38.98896842092991 40.0 38.5 41.0 36.0 41.0 16-17 38.85080024308969 40.0 38.0 41.0 36.0 41.0 18-19 39.00118484362032 40.0 39.0 41.0 36.0 41.0 20-21 38.98504226015115 40.0 39.0 41.0 36.0 41.0 22-23 38.9093525405277 40.0 39.0 41.0 36.0 41.0 24-25 38.81519809696334 40.0 38.5 41.0 35.0 41.0 26-27 38.65106337164522 40.0 38.0 41.0 35.0 41.0 28-29 38.46480919718465 40.0 38.0 41.0 34.5 41.0 30-31 38.262875883622996 40.0 38.0 41.0 34.0 41.0 32-33 38.0180429968528 40.0 38.0 41.0 34.0 41.0 34-35 37.794508512146834 40.0 37.5 41.0 33.5 41.0 36-37 37.54277405702552 40.0 37.0 41.0 33.0 41.0 38-39 37.25989063878811 40.0 37.0 41.0 32.5 41.0 40-41 37.063851849368916 39.0 37.0 41.0 32.0 41.0 42-43 36.893885631257135 39.5 36.5 41.0 31.0 41.0 44-45 36.63895443015653 39.0 36.0 41.0 31.0 41.0 46-47 36.26765642886967 39.0 36.0 41.0 30.0 41.0 48-49 35.87239394520135 39.0 35.0 40.0 28.5 41.0 50-51 35.17955354130521 38.5 34.5 39.5 27.5 40.5 52-53 35.31498586713283 38.5 35.0 40.0 28.0 40.5 54-55 35.42833426726604 39.0 35.0 40.0 28.0 41.0 56-57 35.130982968419474 38.0 35.0 40.0 27.0 41.0 58-59 34.8178379445805 38.0 34.5 40.0 26.0 41.0 60-61 34.394737283152125 38.0 34.0 40.0 25.5 41.0 62-63 33.87889837962069 37.0 33.5 40.0 24.0 41.0 64-65 33.37140438945941 36.5 33.0 39.0 22.5 41.0 66-67 32.83193984258922 36.0 32.5 39.0 20.5 40.0 68-69 32.229266511845154 35.0 31.5 38.5 18.5 40.0 70-71 31.666435551721047 35.0 31.0 38.0 13.5 40.0 72-73 31.02138110793809 35.0 30.5 37.0 7.5 39.5 74-75 30.33520727112151 34.5 29.5 36.5 4.5 39.0 76-77 29.674119182443327 34.0 29.0 36.0 2.0 39.0 78-79 28.944613297209933 34.0 28.5 35.5 2.0 37.5 80-81 28.276977052945604 33.5 26.5 35.0 2.0 37.0 82-83 27.53350116189001 33.0 26.0 35.0 2.0 36.5 84-85 26.86407745058962 32.5 25.0 35.0 2.0 36.0 86-87 26.186790305268474 32.0 23.5 35.0 2.0 35.5 88-89 25.356540103604594 31.5 20.5 34.0 2.0 35.0 90-91 24.50537424225753 31.0 17.0 34.0 2.0 35.0 92-93 23.59754684176508 31.0 4.5 34.0 2.0 35.0 94-95 22.62488095090623 30.0 2.0 34.0 2.0 35.0 96-97 21.392283614836924 29.0 2.0 34.0 2.0 35.0 98-99 19.841833396121785 28.0 2.0 34.0 2.0 35.0 100-101 15.965183195332328 13.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24878521942271448 1101 2 0.25074018807108445 1101 3 0.13964895909427355 1101 4 0.14853520793330688 1101 5 0.13221584760103156 1101 6 0.12575883652813502 1101 7 0.06631447920193523 1101 8 0.07235148057105789 1101 9 0.12476722446394461 1101 10-11 0.1780714853249492 1101 12-13 0.2711934232815949 1101 14-15 0.30492246919602195 1101 16-17 0.44651711242866554 1101 18-19 0.40369433265271937 1101 20-21 0.40968406023561244 1101 22-23 0.3649249197912283 1101 24-25 0.3696164381481779 1101 26-27 0.5318056287683817 1101 28-29 0.6131462820567322 1101 30-31 0.6102235089437613 1101 32-33 0.540041727878041 1101 34-35 0.6076244197342433 1101 36-37 0.5778693358404112 1101 38-39 0.6382345711329052 1101 40-41 0.8564135458835835 1101 42-43 0.9994640885583905 1101 44-45 0.9198195683526791 1101 46-47 0.9628908829626326 1101 48-49 0.978911557101668 1101 50-51 1.0301718095005583 1101 52-53 1.0737906195820344 1101 54-55 1.1371823527041798 1101 56-57 1.251749446336028 1101 58-59 1.196537532589467 1101 60-61 1.1872782632959868 1101 62-63 1.3137994954647354 1101 64-65 1.3619315470111815 1101 66-67 1.324867063169897 1101 68-69 1.583391456717969 1101 70-71 1.793196141261923 1101 72-73 2.0709529728061966 1101 74-75 2.2945950141413505 1101 76-77 2.2542293763761556 1101 78-79 2.295549150535141 1101 80-81 2.4998656409757807 1101 82-83 2.6024520467221848 1101 84-85 2.755743337748804 1101 86-87 3.062763290542062 1101 88-89 3.5389290673154647 1101 90-91 3.9162764032558535 1101 92-93 4.052114258249311 1101 94-95 4.323963551974511 1101 96-97 4.639139165334839 1101 98-99 4.849751600210965 1101 100-101 4.772251962990673 1102 1 0.2902423821671931 1102 2 0.12186350688796921 1102 3 0.1517679882324927 1102 4 0.10027331772710113 1102 5 0.14767291532460547 1102 6 0.1882871710749896 1102 7 0.017108247204390636 1102 8 0.18184389721597682 1102 9 0.2416343508944152 1102 10-11 0.2118917721736011 1102 12-13 0.22976480973963476 1102 14-15 0.312394632650296 1102 16-17 0.36194249697712166 1102 18-19 0.2781850978800975 1102 20-21 0.26446550070673425 1102 22-23 0.31940990648829626 1102 24-25 0.21708212758780832 1102 26-27 0.3671171570937659 1102 28-29 0.335125561775385 1102 30-31 0.36687103691195944 1102 32-33 0.3640394004382941 1102 34-35 0.3429783210865125 1102 36-37 0.4333119157381091 1102 38-39 0.3819377905098378 1102 40-41 0.4658802754014246 1102 42-43 0.5595508580994917 1102 44-45 0.5498804332489797 1102 46-47 0.4968799607103378 1102 48-49 0.6053046007700758 1102 50-51 0.7036179458131997 1102 52-53 0.5548424637889653 1102 54-55 0.5741957998794334 1102 56-57 0.697056472616751 1102 58-59 0.8051988740785632 1102 60-61 0.7599505802719833 1102 62-63 0.7641510728117495 1102 64-65 0.7912837190974713 1102 66-67 0.8831784157638509 1102 68-69 1.1136923209502712 1102 70-71 1.4215945696390548 1102 72-73 1.4826103634863657 1102 74-75 1.4766883389800398 1102 76-77 1.6637231095757734 1102 78-79 1.8873215995100594 1102 80-81 2.039831805519931 1102 82-83 2.1589645133503836 1102 84-85 2.1932977887340463 1102 86-87 2.3092958145949467 1102 88-89 2.8174376283555205 1102 90-91 2.956826431189711 1102 92-93 3.2340040895242375 1102 94-95 3.5658960052768904 1102 96-97 3.7963563321778118 1102 98-99 3.9539707144100653 1102 100-101 4.002858110499751 1103 1 0.41158641554451947 1103 2 0.31918193253401483 1103 3 0.30388839565723913 1103 4 0.19511425390999193 1103 5 0.2395337331542322 1103 6 0.2570906535561477 1103 7 0.27724863206538686 1103 8 0.25130992386674933 1103 9 0.34656112512916337 1103 10-11 0.34232250570844514 1103 12-13 0.43093942871021085 1103 14-15 0.5672280121045077 1103 16-17 0.6000113802842009 1103 18-19 0.5812451738731212 1103 20-21 0.5990582888203235 1103 22-23 0.5925989524246944 1103 24-25 0.7030196941083133 1103 26-27 0.6389684757961334 1103 28-29 0.7302267261501925 1103 30-31 0.7361123573172605 1103 32-33 0.7312099377110641 1103 34-35 0.82059816441744 1103 36-37 0.7689317803555937 1103 38-39 0.9373034669967026 1103 40-41 1.0080935188345208 1103 42-43 1.1137803644641764 1103 44-45 1.0206925687166404 1103 46-47 1.1557102908648815 1103 48-49 1.2778714139592253 1103 50-51 1.1526812041468801 1103 52-53 1.2058180534266754 1103 54-55 1.2575316166447266 1103 56-57 1.4544894731701987 1103 58-59 1.46396933578405 1103 60-61 1.4813421092855705 1103 62-63 1.641801249490193 1103 64-65 1.7344865214730518 1103 66-67 1.765333360631665 1103 68-69 1.905125944135584 1103 70-71 2.085570260387925 1103 72-73 2.2093740879957124 1103 74-75 2.418043201894598 1103 76-77 2.448702248747299 1103 78-79 2.8016378752135793 1103 80-81 2.917443400308983 1103 82-83 3.154622720383461 1103 84-85 3.268736690986163 1103 86-87 3.3212249356728982 1103 88-89 3.6663270021049357 1103 90-91 3.908560487556521 1103 92-93 3.9660987668199965 1103 94-95 4.302503433221883 1103 96-97 4.400256695113654 1103 98-99 4.720735875762188 1103 100-101 4.5526087916696625 1104 1 0.2978308675968009 1104 2 0.22088849959235546 1104 3 0.21949295383885925 1104 4 0.16499822079959614 1104 5 0.17098371909331433 1104 6 0.13887290501420324 1104 7 0.1504090976650545 1104 8 0.17140021939048466 1104 9 0.19159578833969704 1104 10-11 0.2363982756963452 1104 12-13 0.2569455482646674 1104 14-15 0.4401145955468593 1104 16-17 0.47262484574937247 1104 18-19 0.40135793629257677 1104 20-21 0.37138747927846083 1104 22-23 0.30314693070975096 1104 24-25 0.3821913534184702 1104 26-27 0.4022574153586618 1104 28-29 0.4868131946598595 1104 30-31 0.4816817937802611 1104 32-33 0.5375060177726851 1104 34-35 0.6059827609914592 1104 36-37 0.5871478477012531 1104 38-39 0.5773614835519041 1104 40-41 0.6891346684059769 1104 42-43 0.6730478825293957 1104 44-45 0.7971052320983532 1104 46-47 0.7709147243323002 1104 48-49 0.887392945498469 1104 50-51 0.875670646234262 1104 52-53 0.8720188249429199 1104 54-55 1.0178439217787982 1104 56-57 1.1398184374310247 1104 58-59 1.1093689714229598 1104 60-61 1.1338675717673752 1104 62-63 1.2349988236768041 1104 64-65 1.3431575205315838 1104 66-67 1.4158513219213589 1104 68-69 1.4858424699893291 1104 70-71 1.6449718156473097 1104 72-73 1.8971988645494449 1104 74-75 2.027421678914166 1104 76-77 2.031819370050197 1104 78-79 2.1854195275544157 1104 80-81 2.3178086784094134 1104 82-83 2.5477551864232986 1104 84-85 2.6423932623239033 1104 86-87 2.7754513102801077 1104 88-89 3.0890972773320478 1104 90-91 3.164415177177119 1104 92-93 3.5647750540050005 1104 94-95 3.7373864219609274 1104 96-97 4.048776783880818 1104 98-99 4.281021434591828 1104 100-101 4.47804284598147 1105 1 0.2938674975376401 1105 2 0.22211758055122743 1105 3 0.10430274940014073 1105 4 0.05013050152929566 1105 5 0.048466100087019015 1105 6 0.10591784492117995 1105 7 0.13098379404996052 1105 8 0.13930194996975587 1105 9 0.2152755663919237 1105 10-11 0.16527823109758089 1105 12-13 0.13952552470529866 1105 14-15 0.23322062814722244 1105 16-17 0.37456314445883976 1105 18-19 0.2913795581326539 1105 20-21 0.29194255078268583 1105 22-23 0.2528520225996047 1105 24-25 0.27807791423835937 1105 26-27 0.2935641637738655 1105 28-29 0.40440771443273604 1105 30-31 0.32449535503639737 1105 32-33 0.3648522964745169 1105 34-35 0.29674141537365983 1105 36-37 0.35678431788534937 1105 38-39 0.4565060955058442 1105 40-41 0.7126583745724133 1105 42-43 0.6892315742654276 1105 44-45 0.639863044904807 1105 46-47 0.6404518220133326 1105 48-49 0.6910514226505171 1105 50-51 0.6874843846016674 1105 52-53 0.5530229864636524 1105 54-55 0.7723683423336851 1105 56-57 0.8483613227282945 1105 58-59 0.8872522895611752 1105 60-61 0.9282256860196085 1105 62-63 0.9429985479062211 1105 64-65 1.0805591435365542 1105 66-67 1.2767390892375445 1105 68-69 1.4002124352446188 1105 70-71 1.5223248697470808 1105 72-73 1.6352108677017867 1105 74-75 1.851265076883216 1105 76-77 1.938905425924645 1105 78-79 2.1067732639486465 1105 80-81 2.249155056959218 1105 82-83 2.4449417915804474 1105 84-85 2.531600350578813 1105 86-87 2.6384940701730812 1105 88-89 3.0175789700879605 1105 90-91 3.248653521412507 1105 92-93 3.4958838181605323 1105 94-95 3.799268627507484 1105 96-97 3.956580081003416 1105 98-99 4.26144065342411 1105 100-101 4.340334131036727 1106 1 0.1692452767278354 1106 2 0.1440938677515149 1106 3 0.07482390710993059 1106 4 0.09004943115561304 1106 5 0.16158908223595603 1106 6 -0.023471149747130937 1106 7 0.12169582429909553 1106 8 0.16099427504763497 1106 9 0.11346114720634404 1106 10-11 0.07876043772292007 1106 12-13 0.1847087874107629 1106 14-15 0.3047713279021309 1106 16-17 0.1426907055518427 1106 18-19 0.17599295426248318 1106 20-21 0.20670399212495028 1106 22-23 0.1517364511897128 1106 24-25 0.1964734588777617 1106 26-27 0.28059062726023853 1106 28-29 0.22540687168883267 1106 30-31 0.2789353062433477 1106 32-33 0.39476122629886135 1106 34-35 0.3315952846258625 1106 36-37 0.441469762867051 1106 38-39 0.2586052950117974 1106 40-41 0.4280574055651627 1106 42-43 0.30745252257583644 1106 44-45 0.4824427068515007 1106 46-47 0.703514251584572 1106 48-49 0.6727973669677638 1106 50-51 0.6657051408134222 1106 52-53 0.6396408608636293 1106 54-55 0.38631942381944384 1106 56-57 0.7156518673125447 1106 58-59 0.8529448935378312 1106 60-61 0.8171464200688732 1106 62-63 0.6627962229670814 1106 64-65 0.895519288402653 1106 66-67 1.0103794879157633 1106 68-69 0.81344930106264 1106 70-71 0.9964819687349262 1106 72-73 1.3180195918738562 1106 74-75 1.5831929516661987 1106 76-77 1.8133206367226755 1106 78-79 1.939655027110696 1106 80-81 2.1214733094022193 1106 82-83 2.1166437156618727 1106 84-85 2.3998989387604546 1106 86-87 2.2386106100210412 1106 88-89 2.596094194558198 1106 90-91 2.798256831770285 1106 92-93 3.090774088921208 1106 94-95 3.2934949365560264 1106 96-97 3.353185413258778 1106 98-99 3.608486668284243 1106 100-101 3.6737199752871703 1107 1 0.16486863902920135 1107 2 0.06996897077152653 1107 3 0.013285355232177665 1107 4 0.05632207915037668 1107 5 0.07952482262023608 1107 6 0.11830879191458621 1107 7 0.0761360528901065 1107 8 0.0953128162543706 1107 9 0.15840918873811205 1107 10-11 0.12408072143294646 1107 12-13 0.12287289082983932 1107 14-15 0.2312534401283699 1107 16-17 0.2500491262962612 1107 18-19 0.18355587217139657 1107 20-21 0.1897897128320878 1107 22-23 0.16213839214540116 1107 24-25 0.17804706807022086 1107 26-27 0.3092850531610196 1107 28-29 0.290741303595226 1107 30-31 0.37656361811067285 1107 32-33 0.38750509443509173 1107 34-35 0.41827400013359295 1107 36-37 0.4317501315767842 1107 38-39 0.4502452515079298 1107 40-41 0.3727899772508536 1107 42-43 0.4955241123573515 1107 44-45 0.48407024452878744 1107 46-47 0.6251513434393843 1107 48-49 0.6854342002219198 1107 50-51 0.49135884436403643 1107 52-53 0.6577098623357855 1107 54-55 0.7559379452454138 1107 56-57 0.7068850631630568 1107 58-59 0.7172294597300422 1107 60-61 0.7555702755306726 1107 62-63 0.7737407992960712 1107 64-65 0.9628440012534512 1107 66-67 1.2357357431387257 1107 68-69 1.332255072624072 1107 70-71 1.2985547117418292 1107 72-73 1.458582924510985 1107 74-75 1.5429316728893951 1107 76-77 1.729617440736309 1107 78-79 1.8057943564484198 1107 80-81 1.9503672930336968 1107 82-83 2.1941417047711695 1107 84-85 2.393696322495508 1107 86-87 2.472150493818848 1107 88-89 2.800960780336357 1107 90-91 3.2129638059053818 1107 92-93 3.5069972044120767 1107 94-95 3.9052129545281993 1107 96-97 4.191951010758714 1107 98-99 4.384805502863411 1107 100-101 4.121408780826151 1108 1 0.17408922065639132 1108 2 0.07165551797278624 1108 3 0.034242729249868376 1108 4 0.06125780354797428 1108 5 0.03568436388290763 1108 6 0.08945353445545834 1108 7 0.0801712098149494 1108 8 0.029024574515496226 1108 9 0.11012856466955157 1108 10-11 0.03377778612080107 1108 12-13 0.07988004114898928 1108 14-15 0.05536267956648544 1108 16-17 0.19478150354633783 1108 18-19 0.18669754103871838 1108 20-21 0.09544514485093458 1108 22-23 0.0975441611773391 1108 24-25 0.10859635825183744 1108 26-27 0.1745587323781237 1108 28-29 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3.17281671625506 1108 96-97 3.533611931574413 1108 98-99 3.76169414267396 1108 100-101 3.60720280913635 1109 1 0.13534289558674573 1109 2 0.04222255526622121 1109 3 0.01962793933726914 1109 4 0.06852903795488174 1109 5 0.08398494200979201 1109 6 0.0534464653105573 1109 7 0.035172397023444546 1109 8 -0.01627473190252715 1109 9 0.03919126706219345 1109 10-11 0.05997596942773242 1109 12-13 0.10884798242381777 1109 14-15 0.1797346527371957 1109 16-17 0.1663672875248352 1109 18-19 0.13944583440628833 1109 20-21 0.17002328704369774 1109 22-23 0.11624908107067 1109 24-25 0.09656912240625815 1109 26-27 0.07129977768421725 1109 28-29 0.033912479837411524 1109 30-31 -0.07964531427727195 1109 32-33 -0.11771183476783875 1109 34-35 -0.18718483070177427 1109 36-37 -0.11149957720385117 1109 38-39 -0.11334922473094622 1109 40-41 -0.01783062864712548 1109 42-43 0.08177229790391749 1109 44-45 0.28083993289182985 1109 46-47 0.3981551605927436 1109 48-49 0.347039535102887 1109 50-51 0.37721379909201147 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1200.0 5 1300.0 6 1495.0 7 1746.0 8 2168.0 9 2935.0 10 3808.0 11 5165.0 12 7407.0 13 10131.0 14 12601.0 15 16944.0 16 20994.0 17 27199.0 18 25945.0 19 20845.0 20 20336.0 21 21380.0 22 23724.0 23 26801.0 24 31388.0 25 35462.0 26 41243.0 27 48701.0 28 57987.0 29 68945.0 30 84432.0 31 104401.0 32 131713.0 33 171255.0 34 229211.0 35 316283.0 36 422190.0 37 472728.0 38 252462.0 39 15427.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46431881439461 18.60170190750191 14.829909869958518 23.104069408144966 2 15.227688734639786 19.77195042742893 40.73597816602481 24.26438267190647 3 19.63611284562554 23.52322347751249 29.7053974322382 27.13526624462376 4 12.63335790333999 16.653215134832468 37.488033969310095 33.22539299251745 5 13.68007018505986 37.20934896501832 34.37179120673963 14.73878964318219 6 32.02271342513944 36.31650318557501 17.294830044933143 14.3659533443524 7 28.46865893065106 32.75064745238195 20.39082248778452 18.38987112918247 8 26.239876365916064 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29.251086098936018 22.5229316916352 32-33 21.516275016142973 26.40680806144518 29.46995456638868 22.606962356023168 34-35 22.48953559283605 26.466648222044093 29.12949036341374 21.91432582170611 36-37 22.424977277991808 25.942327743303185 29.360497804708647 22.272197173996364 38-39 21.811348671773 26.604099341193578 29.191055684676716 22.39349630235671 40-41 22.4358947494053 26.421940782191104 29.117363286926402 22.024801181477194 42-43 21.983978350146153 26.430638508154225 28.848171507891472 22.737211633808144 44-45 22.0409996383403 26.603013344969607 29.017697639737744 22.338289376952346 46-47 22.663737069505103 26.97485868453483 28.28845793262595 22.072946313334118 48-49 22.23078034600452 26.973031705935334 28.319688015389318 22.47649993267083 50-51 22.178059327115385 27.19460386308219 28.49027214809678 22.137064661705644 52-53 22.655649031234933 26.98259681000427 28.10049095077119 22.2612632079896 54-55 22.05064005180322 27.07337684036074 28.441556122644197 22.434426985191845 56-57 22.335586033695034 27.10032788414517 28.171435526199755 22.392650555960035 58-59 22.50310921365773 26.774784341005216 28.059245779285728 22.662860666051323 60-61 21.970317423732396 27.06128647576117 28.582493456659996 22.385902643846435 62-63 21.994113151031108 27.057076562743404 28.645724108842025 22.303086177383456 64-65 21.725639700251563 27.350159033420795 28.29506734938286 22.62913391694478 66-67 21.807805726599526 27.066419474271626 28.23925337985681 22.886521419272043 68-69 22.26056579370009 26.779904599995735 28.417985456974254 22.54154414932992 70-71 22.28630160972434 26.87348849768718 27.95030037364729 22.889909518941188 72-73 22.175184090969136 26.888550603038556 28.23577855698917 22.700486749003133 74-75 22.101802577859793 27.556998307760256 27.840696252439614 22.500502861940348 76-77 22.22574051064171 26.99002328386272 27.933730264284595 22.85050594121098 78-79 22.358906433190707 27.255743448519627 27.850535059199494 22.534815059090178 80-81 22.666291819153443 27.120733816845327 27.534147138702448 22.678827225298782 82-83 22.460775480792446 27.19743395167616 27.803982622146467 22.53780794538493 84-85 22.445728378075053 27.470426447996317 27.61024128170521 22.473603892223423 86-87 22.56926315206504 27.412883407194354 27.324436004341667 22.69341743639894 88-89 22.45691668259595 27.492890134237836 27.040103706459988 23.010089476706227 90-91 22.507466766284292 27.265696609659873 27.192149645112206 23.03468697894363 92-93 22.75859744315516 26.957412946019836 27.462076869327245 22.82191274149776 94-95 22.476128414089168 26.861367765928616 27.5463313501316 23.116172469850614 96-97 22.586621947670686 26.56129422206932 27.65783408185901 23.19424974840098 98-99 22.517928797918696 26.821988237090515 27.03916730349932 23.620915661491466 100-101 23.12203138260493 25.723450554117587 27.28455381256399 23.86996425071349 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 687.0 1 473.5 2 348.5 3 452.0 4 382.0 5 390.5 6 740.0 7 1694.0 8 2893.0 9 3406.0 10 2945.0 11 2324.0 12 2250.5 13 2458.5 14 2700.0 15 2956.0 16 3170.0 17 3471.5 18 3912.5 19 4292.0 20 4882.0 21 5867.0 22 7208.5 23 8987.5 24 11225.5 25 14185.5 26 18234.0 27 22914.5 28 26573.5 29 30153.0 30 34361.0 31 40420.5 32 48694.0 33 52491.0 34 53046.5 35 55820.5 36 60259.0 37 65071.5 38 68930.0 39 72487.5 40 76929.0 41 81498.5 42 84920.0 43 88404.5 44 91619.5 45 95665.0 46 98299.5 47 103552.5 48 111792.5 49 109460.0 50 100549.5 51 94954.0 52 91851.5 53 91521.5 54 92428.0 55 87238.0 56 79041.5 57 72106.0 58 67059.5 59 60185.0 60 48949.5 61 40346.0 62 34181.0 63 25958.0 64 18320.0 65 13891.5 66 10392.0 67 7547.5 68 5698.0 69 4511.5 70 3513.0 71 2475.5 72 1733.5 73 1347.5 74 1013.5 75 754.5 76 542.0 77 332.5 78 225.5 79 144.0 80 83.5 81 60.0 82 43.5 83 26.0 84 21.5 85 17.0 86 12.5 87 8.5 88 4.0 89 2.5 90 3.0 91 3.0 92 2.5 93 2.0 94 1.5 95 2.5 96 2.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42318446033142104 2 0.038328889562518724 3 0.04893127251184661 4 0.05406850961100549 5 0.08022834108048119 6 0.07822445426875255 7 0.044486287220375814 8 0.0395676559552237 9 0.04466845874871478 10-11 0.04730994590962981 12-13 0.04168084568395572 14-15 0.03670756296030191 16-17 0.03754555199066116 18-19 0.04485063027705375 20-21 0.053813469471330935 22-23 0.045032801805392715 24-25 0.06324995463928945 26-27 0.05487006433569695 28-29 0.04317465221633525 30-31 0.045415362014904544 32-33 0.04417659562219957 34-35 0.04805684917581957 36-37 0.04301069784083018 38-39 0.025085019452275797 40-41 0.02344547569722509 42-43 0.022917178265042085 44-45 0.013608213166920857 46-47 0.026105180010974012 48-49 0.024939282229604624 50-51 0.01481054525395804 52-53 0.014537287961449591 54-55 0.015848922965490155 56-57 0.014719459489788558 58-59 0.014683025184120764 60-61 0.016887300677022266 62-63 0.015065585393632595 64-65 0.015721402895652877 66-67 0.008926404888609397 68-69 0.011895800800534564 70-71 0.018235369986730626 72-73 0.015976443035327432 74-75 0.013626430319754754 76-77 0.010055868364310994 78-79 0.012515183996887053 80-81 0.015994660188161328 82-83 0.01342604163858189 84-85 0.011731846425029493 86-87 0.003534127649775966 88-89 0.02796332960003148 90-91 0.0306412510666143 92-93 0.016887300677022266 94-95 0.03171606308381421 96-97 0.026105180010974012 98-99 0.022479966597028566 100-101 0.14253100377240802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2744666.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.98115742270304 #Duplication Level Percentage of deduplicated Percentage of total 1 74.10395661943096 38.520094346797976 2 14.36707657749387 14.936345385574768 3 4.935870453008941 7.697167771077789 4 2.250524837914923 4.679395435334354 5 1.1994082987970192 3.117331579693215 6 0.7357142568392557 2.2945967161732996 7 0.47691158003110395 1.7353291142814797 8 0.3310060342215063 1.3764861418186198 9 0.2371044449149243 1.1092467129070769 >10 1.2242308453980868 11.462939741519177 >50 0.08741100541112928 3.109505058478396 >100 0.04347554158355685 4.1968907849017825 >500 0.0036547524774405396 1.375077287607454 >1k 0.0033736176714623672 3.185460539434463 >5k 2.1085110446639795E-4 0.7749286451806309 >10k+ 7.028370148879932E-5 0.4292047392193697 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11748 0.4280302229852376 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9195 0.33501344061536087 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6150 0.22407097985692975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5866 0.21372363704727643 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3916 0.14267674099507918 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3520 0.12824875595063295 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3253 0.11852079633733212 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3186 0.11607969785758995 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3084 0.11236339867947502 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3027 0.11028664325641079 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2858 0.1041292455985537 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.6434305667793455E-5 0.0 8 0.0 0.0 0.0 3.6434305667793455E-5 0.0 9 0.0 0.0 0.0 3.6434305667793455E-5 0.0 10-11 0.0 0.0 0.0 5.465145850169018E-5 0.0 12-13 0.0 0.0 0.0 1.0930291700338038E-4 0.0 14-15 0.0 0.0 0.0 1.4573722267117382E-4 0.0 16-17 0.0 0.0 0.0 1.4573722267117382E-4 0.0 18-19 0.0 0.0 0.0 1.4573722267117382E-4 0.0 20-21 0.0 0.0 0.0 1.821715283389673E-4 0.0 22-23 0.0 0.0 0.0 2.1860583400676076E-4 0.0 24-25 0.0 0.0 0.0 4.00777362345728E-4 0.0 26-27 0.0 0.0 0.0 8.74423336027043E-4 0.0 28-29 0.0 0.0 0.0 0.0024593156325760585 0.0 30-31 0.0 0.0 0.0 0.00796089578841287 0.0 32-33 0.0 0.0 0.0 0.01971095936627626 0.0 34-35 0.0 0.0 0.0 0.03741803192082388 0.0 36-37 0.0 0.0 0.0 0.06547244728502484 0.0 38-39 0.0 0.0 0.0 0.1074629845671568 0.0 40-41 0.0 0.0 0.0 0.15286012942922744 0.0 42-43 0.0 0.0 0.0 0.20133597312022666 1.8217152833896728E-5 44-45 0.0 0.0 0.0 0.26332894421397723 5.465145850169018E-5 46-47 0.0 0.0 0.0 0.33603360117405906 9.108576416948365E-5 48-49 0.0 0.0 0.0 0.4125820773820931 1.0930291700338038E-4 50-51 0.0 0.0 0.0 0.49443174506479115 1.0930291700338038E-4 52-53 0.0 0.0 0.0 0.5753523379529604 1.0930291700338038E-4 54-55 0.0 0.0 0.0 0.6627946715556647 1.0930291700338038E-4 56-57 0.0 0.0 0.0 0.769638272926469 1.0930291700338038E-4 58-59 0.0 0.0 0.0 0.8822931460512864 1.4573722267117382E-4 60-61 0.0 0.0 0.0 1.0009961139169574 1.4573722267117382E-4 62-63 0.0 0.0 0.0 1.1197537332411303 1.4573722267117382E-4 64-65 0.0 0.0 0.0 1.2459439509215329 1.4573722267117382E-4 66-67 0.0 0.0 0.0 1.3895497667111407 1.4573722267117382E-4 68-69 0.0 0.0 0.0 1.547565350392361 1.4573722267117382E-4 70-71 0.0 0.0 0.0 1.7116836802729367 1.4573722267117382E-4 72-73 0.0 0.0 0.0 1.8822144479510439 1.4573722267117382E-4 74-75 0.0 0.0 0.0 2.052818084240487 1.4573722267117382E-4 76-77 0.0 0.0 0.0 2.236920630779847 1.4573722267117382E-4 78-79 0.0 0.0 0.0 2.4487132496267305 1.4573722267117382E-4 80-81 0.0 0.0 0.0 2.671600114549457 1.821715283389673E-4 82-83 0.0 0.0 0.0 2.8983125815673016 1.821715283389673E-4 84-85 0.0 0.0 0.0 3.1326033841640477 1.821715283389673E-4 86-87 0.0 0.0 0.0 3.3757112887323997 1.821715283389673E-4 88-89 0.0 0.0 0.0 3.6300227422935976 1.821715283389673E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11975 0.0 26.46413 1 GTATCAA 16340 0.0 25.674494 1 TAAGGTG 1780 0.0 25.099386 5 GGTAAGG 1710 0.0 23.903284 3 GTAAGGT 1750 0.0 23.62852 4 AGGTAAG 1745 0.0 23.423424 2 GAGTACT 10820 0.0 22.369404 12-13 TATCAAC 18920 0.0 22.005455 2 CAACGCA 18970 0.0 21.972908 5 ATCAACG 18960 0.0 21.93436 3 TCAACGC 19085 0.0 21.815603 4 AACGCAG 19450 0.0 21.453125 6 GTACTTT 11345 0.0 21.230913 14-15 AAGGTAA 1835 0.0 21.067549 1 AGAGTAC 14695 0.0 20.866627 10-11 GTACATG 8595 0.0 20.601227 1 TACATGG 8420 0.0 20.546444 2 ACATGGG 8220 0.0 19.832504 3 GGACCGA 700 0.0 19.688635 6 AGTACTT 11215 0.0 19.654238 12-13 >>END_MODULE