FastQCFastQC Report
Thu 26 May 2016
SRR937954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2744666
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111700.40697119430925294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97120.35384997664561No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69040.25154244633044603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62490.2276779761180413No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32330.11779211022397625No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31240.11382077090618677No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31070.11320138770983428No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31070.11320138770983428No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29890.10890213964103465No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29050.10584165796494001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA179800.024.0693361
TAAGGTG17150.023.8171945
GAGTACT112100.022.2862212-13
GGTATCA136450.022.2465551
GTACTTT116250.021.3476314-15
GTAAGGT17600.021.3191914
AGAGTAC151200.021.15641810-11
GTACATG83950.021.1068551
AGTACTT116000.020.57474112-13
ATCAACG208850.020.5375733
TCAACGC210150.020.4538694
TATCAAC209750.020.4513152
CAACGCA211550.020.3409615
TACATGG81950.020.2307452
ATACCGA1908.264236E-619.9982816
AACGCAG216050.019.8733226
ACATGGG80950.019.2465483
CGTAGCG1250.00603304819.00013
GGTAAGG19550.018.7085363
AGGTAAG18500.018.4882662
Produced by FastQC (version 0.11.5)