##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937954_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2744666 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00122637872878 31.0 31.0 34.0 30.0 34.0 2 32.29295914329831 33.0 31.0 34.0 31.0 34.0 3 32.276386635022256 34.0 31.0 34.0 30.0 34.0 4 35.9339999839689 37.0 35.0 37.0 35.0 37.0 5 35.862928312588856 37.0 35.0 37.0 35.0 37.0 6 35.93961196007091 37.0 35.0 37.0 35.0 37.0 7 35.88953701470416 37.0 35.0 37.0 35.0 37.0 8 35.876776627830125 37.0 35.0 37.0 35.0 37.0 9 37.48883069925448 39.0 37.0 39.0 35.0 39.0 10-11 37.41925155920611 39.0 37.0 39.0 34.5 39.0 12-13 37.344500205125144 39.0 37.0 39.0 34.0 39.0 14-15 38.408907495483966 40.0 38.0 41.0 34.0 41.0 16-17 38.29641548370549 40.0 38.0 41.0 33.0 41.0 18-19 38.279396655185 40.0 38.0 41.0 33.5 41.0 20-21 38.263912075276195 40.0 38.0 41.0 34.0 41.0 22-23 38.28405131990559 40.0 38.0 41.0 34.0 41.0 24-25 38.18934435009579 40.0 38.0 41.0 33.5 41.0 26-27 37.94631678317143 40.0 37.0 41.0 33.0 41.0 28-29 37.835767084228095 40.0 37.0 41.0 33.0 41.0 30-31 37.54911071146726 39.0 37.0 41.0 32.0 41.0 32-33 37.280227539525754 39.0 36.0 40.0 32.0 41.0 34-35 36.99905143285194 39.0 36.0 40.0 31.0 41.0 36-37 36.86736692187684 39.0 36.0 40.0 31.0 41.0 38-39 36.77236574504876 39.0 35.0 40.0 31.0 41.0 40-41 36.6021734520703 39.0 35.0 40.0 30.0 41.0 42-43 36.33812019385965 39.0 35.0 40.0 30.0 41.0 44-45 35.99572115514238 38.0 35.0 40.0 29.5 41.0 46-47 35.53580144177835 38.0 35.0 40.0 27.5 41.0 48-49 35.04138080917678 38.0 34.0 40.0 26.5 41.0 50-51 34.6221975642938 38.0 33.5 40.0 25.5 41.0 52-53 33.98396052561587 37.5 33.0 40.0 23.5 41.0 54-55 33.517950271544876 37.0 32.5 39.5 22.5 40.5 56-57 33.126149046914996 37.0 32.0 39.0 21.5 40.0 58-59 32.601174241237366 36.0 31.0 39.0 19.0 40.0 60-61 32.00076038395929 35.5 30.5 39.0 17.5 40.0 62-63 31.417090276193896 35.0 29.5 38.0 13.0 40.0 64-65 30.829341347909 34.5 29.0 38.0 8.0 40.0 66-67 30.12959445703047 34.0 28.0 37.5 7.5 39.5 68-69 29.421418125192645 34.0 27.5 37.0 4.5 39.0 70-71 28.63041969405385 33.0 26.0 36.0 2.0 39.0 72-73 28.000757287043307 32.5 26.0 35.5 2.0 38.5 74-75 27.30574357681408 32.0 25.0 35.0 2.0 37.5 76-77 26.427748585802426 30.5 24.0 34.0 2.0 36.0 78-79 27.112460131761022 32.0 25.5 35.0 2.0 36.5 80-81 26.779448574070578 32.0 25.0 35.0 2.0 36.0 82-83 26.09479641603022 31.5 23.0 34.5 2.0 36.0 84-85 25.320093956787456 31.0 20.5 34.0 2.0 35.5 86-87 24.493797241631587 31.0 18.0 34.0 2.0 35.0 88-89 23.516674342160393 30.0 9.0 34.0 2.0 35.0 90-91 22.630006529027575 29.0 4.0 34.0 2.0 35.0 92-93 21.626561301083626 29.0 2.0 34.0 2.0 35.0 94-95 20.45775478692125 27.5 2.0 33.0 2.0 34.5 96-97 19.165086389382168 25.5 2.0 32.5 2.0 34.0 98-99 17.70769630986065 24.0 2.0 32.0 2.0 34.0 100-101 14.728732931438653 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08294417589164027 1101 2 0.09574421863676008 1101 3 0.18503584324733424 1101 4 0.07217191625840513 1101 5 0.004431707148981445 1101 6 0.05916129327574993 1101 7 0.019327143874072306 1101 8 0.11229292309209171 1101 9 0.21825938506164277 1101 10-11 0.17855514216302026 1101 12-13 0.15716149670124224 1101 14-15 0.2535809095937722 1101 16-17 0.40850833516059737 1101 18-19 0.3993288390630241 1101 20-21 0.37057785495203177 1101 22-23 0.2155937767829883 1101 24-25 0.1886968806071252 1101 26-27 0.32389136727517354 1101 28-29 0.45043449181557804 1101 30-31 0.39697389131127636 1101 32-33 0.43181748407067744 1101 34-35 0.319497251605533 1101 36-37 0.31320109775787586 1101 38-39 0.4529473941667703 1101 40-41 0.5274828952611799 1101 42-43 0.5634609676702169 1101 44-45 0.6364601883485648 1101 46-47 0.6428544667517428 1101 48-49 0.7025884122264685 1101 50-51 0.7934147353317726 1101 52-53 0.7632692502440364 1101 54-55 0.8914632985011934 1101 56-57 0.9602979247253742 1101 58-59 1.1534289698521505 1101 60-61 1.0113883619851407 1101 62-63 1.167094550212692 1101 64-65 1.2087519921912069 1101 66-67 1.2297925772843321 1101 68-69 1.4842851146309286 1101 70-71 1.5186852953048842 1101 72-73 1.5929284989349064 1101 74-75 1.7026894495523273 1101 76-77 1.9269887943679578 1101 78-79 1.8738670505073323 1101 80-81 2.1571618404197395 1101 82-83 2.2490287901477686 1101 84-85 2.53153853521275 1101 86-87 2.5086870977043816 1101 88-89 2.9756901973523284 1101 90-91 3.244377466102982 1101 92-93 3.5313976623189127 1101 94-95 3.7070398092214027 1101 96-97 3.875730921098647 1101 98-99 4.073539720193242 1101 100-101 3.780843020043969 1102 1 0.1378156000145907 1102 2 0.07202577015640799 1102 3 0.23288029975065427 1102 4 0.06593674180918185 1102 5 0.10121622263395835 1102 6 0.1094878847041798 1102 7 0.16548847105279663 1102 8 0.19700677851378146 1102 9 0.15582492658921154 1102 10-11 0.17093593958303188 1102 12-13 0.20535290829973718 1102 14-15 0.18066467223841443 1102 16-17 0.2715976651143919 1102 18-19 0.36320823063157803 1102 20-21 0.3833051466730737 1102 22-23 0.30940502519739965 1102 24-25 0.2911544589249573 1102 26-27 0.36164679319779225 1102 28-29 0.18629870121875314 1102 30-31 0.1483140581359237 1102 32-33 0.19324839872129473 1102 34-35 0.22385592060307857 1102 36-37 0.3857780360698442 1102 38-39 0.4270004621971637 1102 40-41 0.40376431798743084 1102 42-43 0.43623420142622393 1102 44-45 0.5711912734644002 1102 46-47 0.6235957042215858 1102 48-49 0.5555039941665498 1102 50-51 0.6932610561910337 1102 52-53 0.6878086278227613 1102 54-55 0.8417788852495605 1102 56-57 1.0516886443885198 1102 58-59 1.009914203832544 1102 60-61 1.0646661825032453 1102 62-63 1.11956170217287 1102 64-65 1.0709534347917113 1102 66-67 1.2563296692096166 1102 68-69 1.3039491041122275 1102 70-71 1.6861333085665997 1102 72-73 1.5864723722617349 1102 74-75 1.6758841811583416 1102 76-77 1.8299849723524133 1102 78-79 1.9974643473343185 1102 80-81 2.2013977922086525 1102 82-83 2.30223434326102 1102 84-85 2.426398262033377 1102 86-87 2.8394887822075034 1102 88-89 2.9373813891434395 1102 90-91 3.114313095164267 1102 92-93 3.5097502116267485 1102 94-95 3.478534925948285 1102 96-97 3.696992020227203 1102 98-99 3.545361871970247 1102 100-101 3.3588414447576493 1103 1 0.4522899284236317 1103 2 0.4136524358297038 1103 3 0.5030839056938632 1103 4 0.2851710110415908 1103 5 0.21029919764353622 1103 6 0.26405624649054715 1103 7 0.3747326917518521 1103 8 0.35301730471753245 1103 9 0.47297870069624537 1103 10-11 0.4781016607716779 1103 12-13 0.46652236527960156 1103 14-15 0.6429315844100714 1103 16-17 0.659488007402274 1103 18-19 0.6826616183875558 1103 20-21 0.6847394616715903 1103 22-23 0.7175601722875271 1103 24-25 0.739903075298578 1103 26-27 0.8735878879655559 1103 28-29 0.8794343178903858 1103 30-31 0.8982754762465319 1103 32-33 0.9191353830543818 1103 34-35 1.0366452847735346 1103 36-37 1.0485630098873742 1103 38-39 1.0069813841180562 1103 40-41 1.1420134718466883 1103 42-43 1.0720218307420808 1103 44-45 1.1138189396059985 1103 46-47 1.0559643129320477 1103 48-49 1.1203062686354457 1103 50-51 1.2139535878015764 1103 52-53 1.3247956217348076 1103 54-55 1.5622138494881384 1103 56-57 1.6843564623032918 1103 58-59 1.789026234996065 1103 60-61 1.99029892548932 1103 62-63 1.9451376366528415 1103 64-65 1.9872753035387838 1103 66-67 2.01286530823214 1103 68-69 2.25367357620555 1103 70-71 2.2870901394235936 1103 72-73 2.3126833114247454 1103 74-75 2.2927334244211472 1103 76-77 2.1950394925533594 1103 78-79 2.5232184237104924 1103 80-81 2.7776462489330775 1103 82-83 2.855111487881903 1103 84-85 3.066276902035078 1103 86-87 3.2835969285738855 1103 88-89 3.2699938620030764 1103 90-91 3.383246824782386 1103 92-93 3.6432156103240096 1103 94-95 4.015000798271505 1103 96-97 4.198859915837918 1103 98-99 4.3706936762190445 1103 100-101 4.025664212861868 1104 1 0.11912787332929753 1104 2 0.1429550756135285 1104 3 0.13156031392500012 1104 4 0.05251514886300157 1104 5 0.002943199746560765 1104 6 0.09983760538621311 1104 7 0.021347373791783752 1104 8 0.01333098537583055 1104 9 0.039132551552732764 1104 10-11 0.1324128593729199 1104 12-13 0.11089249745219831 1104 14-15 0.2437764619578573 1104 16-17 0.23761701934794388 1104 18-19 0.20464356539624617 1104 20-21 0.3464277495412986 1104 22-23 0.32009388791470883 1104 24-25 0.26628135057460156 1104 26-27 0.38087556815922596 1104 28-29 0.3337692060755515 1104 30-31 0.4078653254890767 1104 32-33 0.4429160572446591 1104 34-35 0.41524044659632153 1104 36-37 0.5124255430527924 1104 38-39 0.5284560683588282 1104 40-41 0.6052475432761284 1104 42-43 0.5870277302126539 1104 44-45 0.694678214307352 1104 46-47 0.7929627050970538 1104 48-49 0.7375509102043978 1104 50-51 0.8390740225895001 1104 52-53 0.8513154231702487 1104 54-55 0.9686659751315787 1104 56-57 1.1068452135718303 1104 58-59 1.096108809243809 1104 60-61 1.3535617300915135 1104 62-63 1.166651141244035 1104 64-65 1.3335661260411378 1104 66-67 1.3910655417655882 1104 68-69 1.458397737747113 1104 70-71 1.7085006325337453 1104 72-73 1.6250226807621715 1104 74-75 1.5594306073475295 1104 76-77 1.693067449319102 1104 78-79 1.9152244882439788 1104 80-81 2.1786578705844377 1104 82-83 2.3267770643182573 1104 84-85 2.5855189576184827 1104 86-87 2.604753561046042 1104 88-89 2.9329942471498605 1104 90-91 2.952082642210584 1104 92-93 3.2594617741397762 1104 94-95 3.268916304403284 1104 96-97 3.4003855250423136 1104 98-99 3.6160842945324667 1104 100-101 3.631478706607817 1105 1 0.2329514681177116 1105 2 0.20465612841527303 1105 3 0.23499758027777773 1105 4 0.1754769724673011 1105 5 0.19998936397982447 1105 6 0.14719811242371605 1105 7 0.1686539153790534 1105 8 0.14011841261459068 1105 9 0.17131664268065094 1105 10-11 0.200145680470321 1105 12-13 0.18803046238416954 1105 14-15 0.22996225763324674 1105 16-17 0.23069690128959763 1105 18-19 0.3030433828171013 1105 20-21 0.29675487050830185 1105 22-23 0.3197579708170508 1105 24-25 0.3657583968339324 1105 26-27 0.2828807887816396 1105 28-29 0.24603731213786517 1105 30-31 0.36686953217913043 1105 32-33 0.24866313996038514 1105 34-35 0.2982118508510325 1105 36-37 0.24788357778324865 1105 38-39 0.2733704190082591 1105 40-41 0.5519482810813017 1105 42-43 0.546236625240347 1105 44-45 0.582331894002202 1105 46-47 0.5916369261343135 1105 48-49 0.7005102257227946 1105 50-51 0.8022462282430354 1105 52-53 0.8027944212196445 1105 54-55 0.8757737835993922 1105 56-57 0.975625309116829 1105 58-59 1.1416589774995245 1105 60-61 1.1879483010150764 1105 62-63 1.3848310197385665 1105 64-65 1.4811253186514328 1105 66-67 1.6483786923375128 1105 68-69 1.5159953955990275 1105 70-71 1.7756105924875776 1105 72-73 1.7598636788139466 1105 74-75 1.6438843379172923 1105 76-77 1.7429310498606618 1105 78-79 1.993129677842198 1105 80-81 2.305837795436954 1105 82-83 2.303929590412455 1105 84-85 2.707447592470892 1105 86-87 2.8770223327614914 1105 88-89 2.8909496341261196 1105 90-91 3.166482977889949 1105 92-93 3.3726030744693922 1105 94-95 3.5693275235113333 1105 96-97 3.810879724945078 1105 98-99 4.062130552670375 1105 100-101 3.709436197523436 1106 1 0.0870648753347183 1106 2 0.0590305289222286 1106 3 0.09670884485964137 1106 4 0.029726271692609885 1106 5 0.08746757945925765 1106 6 -0.028356338235212775 1106 7 0.09542507975893955 1106 8 0.012879737037550854 1106 9 -0.014280417119067579 1106 10-11 0.0844517780490861 1106 12-13 0.09145268798458517 1106 14-15 0.09112278012734265 1106 16-17 0.10458923616213411 1106 18-19 0.06345634511228582 1106 20-21 -0.0675749832702337 1106 22-23 0.004655174916052829 1106 24-25 0.1403554135893188 1106 26-27 0.13357804166421516 1106 28-29 0.06131637633759368 1106 30-31 0.21929798521462374 1106 32-33 0.24748742507178179 1106 34-35 0.1178442617827713 1106 36-37 0.26238738163296915 1106 38-39 0.3658103054708377 1106 40-41 0.5068521742450471 1106 42-43 0.4075037506275194 1106 44-45 0.577540929943261 1106 46-47 0.19739725318581947 1106 48-49 0.5668196442366238 1106 50-51 0.5745238316629653 1106 52-53 0.5248001042566273 1106 54-55 0.6265984144883632 1106 56-57 0.8047532630634251 1106 58-59 0.8142766311046259 1106 60-61 0.8290420324685144 1106 62-63 0.9541464382521561 1106 64-65 0.974349098045348 1106 66-67 0.9414094125876851 1106 68-69 0.7231160419706697 1106 70-71 1.0178271879848566 1106 72-73 0.9659526249951398 1106 74-75 1.2972449911570543 1106 76-77 1.4470259053283634 1106 78-79 1.7943847697904545 1106 80-81 1.931128329823384 1106 82-83 1.8955253100276366 1106 84-85 2.1538004276513156 1106 86-87 2.3894819460953656 1106 88-89 2.7380924097879813 1106 90-91 2.726345601267383 1106 92-93 2.533118928122004 1106 94-95 3.0122136302373086 1106 96-97 2.971832840186366 1106 98-99 3.242299995538044 1106 100-101 3.04045744968173 1107 1 0.08988254022372644 1107 2 0.07081286315816726 1107 3 0.08061262330772934 1107 4 0.1044501181966524 1107 5 0.0607746029196079 1107 6 0.07954749271594608 1107 7 0.057806743475374844 1107 8 0.1216324827985602 1107 9 0.0399123101461214 1107 10-11 0.08209973251326375 1107 12-13 0.11266749854866731 1107 14-15 0.18737346203402439 1107 16-17 0.1475950669967787 1107 18-19 0.1386665934357012 1107 20-21 0.1915378690904319 1107 22-23 0.09269453748269996 1107 24-25 0.1963917355832976 1107 26-27 0.3025394917438149 1107 28-29 0.27539926625407674 1107 30-31 0.20903300128678381 1107 32-33 0.29913312006162585 1107 34-35 0.29310527459006863 1107 36-37 0.3104127825273224 1107 38-39 0.42698576866653326 1107 40-41 0.4284844878597127 1107 42-43 0.3253416234425117 1107 44-45 0.39305117805943013 1107 46-47 0.6059029351466165 1107 48-49 0.7523838668929415 1107 50-51 0.7383447013118811 1107 52-53 0.5878870937880265 1107 54-55 0.6986416682695591 1107 56-57 0.7800882752669409 1107 58-59 0.7359866055139861 1107 60-61 0.941423880406262 1107 62-63 0.9286057084180008 1107 64-65 0.8403141079714196 1107 66-67 1.0683927564484996 1107 68-69 1.3235461296392828 1107 70-71 1.4659272675914892 1107 72-73 1.2948881735589843 1107 74-75 1.466578856122375 1107 76-77 1.7100552451045274 1107 78-79 2.0199205832184823 1107 80-81 2.120211639994725 1107 82-83 2.2496387875893618 1107 84-85 2.538160395624441 1107 86-87 2.646647118688854 1107 88-89 2.7861293245140075 1107 90-91 2.8146242212563592 1107 92-93 3.122739945746684 1107 94-95 3.312869135702581 1107 96-97 3.249458724937977 1107 98-99 3.310278694028323 1107 100-101 3.2814183434129784 1108 1 -0.018374560429755604 1108 2 -0.018812533700682366 1108 3 0.06538529505269253 1108 4 0.0066406978108801695 1108 5 -0.09101618181107085 1108 6 -0.022585200330929922 1108 7 -0.0672219857794687 1108 8 -0.044008491847030484 1108 9 -0.05252674267421753 1108 10-11 -0.041636152834016116 1108 12-13 -0.07078155304019162 1108 14-15 0.014475483102806663 1108 16-17 0.06005195116283346 1108 18-19 0.12619260891470674 1108 20-21 0.025127433351876505 1108 22-23 0.04267336509202124 1108 24-25 0.05275676647698191 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2316 92-93 -4.13116160721389 2316 94-95 -3.9874324551962523 2316 96-97 -4.2565384379005895 2316 98-99 -4.568673644669106 2316 100-101 -4.351715753275771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 15.0 4 39.0 5 79.0 6 133.0 7 417.0 8 1046.0 9 1899.0 10 3243.0 11 5208.0 12 7993.0 13 11491.0 14 16491.0 15 21718.0 16 27788.0 17 36696.0 18 36461.0 19 31878.0 20 31125.0 21 32750.0 22 35384.0 23 39629.0 24 44920.0 25 50574.0 26 59450.0 27 70802.0 28 85260.0 29 102878.0 30 121804.0 31 146174.0 32 176898.0 33 216619.0 34 268622.0 35 330466.0 36 362198.0 37 283410.0 38 81253.0 39 1849.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.422174501378315 19.708882610853195 15.600222395001795 22.268720492766697 2 16.097972449803148 20.873837879789903 39.3942308918561 23.633958778550852 3 20.3708487656289 23.973488940668936 30.18045082646661 25.47521146723556 4 13.444076619887447 17.654315679940655 37.09139837051211 31.810209329659784 5 13.610062911413573 37.47963768097461 34.48672746609498 14.423571941516833 6 31.85056207367184 37.03714634027263 16.906295793501414 14.205995792554118 7 28.323992791836964 33.025584898126034 20.29073847236786 18.359683837669138 8 26.285403240615246 34.97510077736291 19.267822946633906 19.471673035387937 9 25.567846870985395 17.291685035629108 20.359817915913993 36.7806501774715 10-11 24.87932957962827 26.7094065361687 27.16035393741898 21.250909946784052 12-13 26.283125159855516 23.911379380952 27.486823533355242 22.31867192583724 14-15 22.56057749831856 25.540666878957218 26.28642246451845 25.612333158205768 16-17 21.776948452015652 28.751512934542856 27.142100350279414 22.329438263162075 18-19 21.809393201212828 27.142519344794596 29.601871411676324 21.44621604231626 20-21 22.295645444655197 25.993927858617404 30.069013861795934 21.641412834931465 22-23 22.069843835279045 25.99108598277532 30.034583442939873 21.904486739005765 24-25 22.18540271512996 26.205084856920084 29.938752905684574 21.670759522265378 26-27 21.697532551300412 26.39501408460969 30.015032086672278 21.892421277417615 28-29 21.66483674933084 27.152329824519217 29.616369535091692 21.566463891058255 30-31 21.890629195642596 26.4565449383039 29.501672882973246 22.151152983080255 32-33 21.663027068445277 26.438366970240796 29.594314903248385 22.30429105806554 34-35 22.53127582633335 26.506417684415506 29.34418057553642 21.61812591371472 36-37 22.48689418334024 25.995126594673867 29.556283276932394 21.961695945053496 38-39 21.86860630266302 26.64488098806282 29.3897125073907 22.096800201883458 40-41 22.44732148778943 26.502533801635366 29.24376243122512 21.806382279350082 42-43 22.054807873632694 26.493168830151347 29.001128735006 22.45089456120996 44-45 22.236580571228686 26.530815996735214 29.20699524674478 22.025608185291325 46-47 22.76309238381044 27.014827733014457 28.417462450637526 21.804617432537576 48-49 22.39021911817509 27.028588781745498 28.39236401004787 22.18882809003154 50-51 22.310601727131825 27.24475400649842 28.581218989851585 21.863425276518164 52-53 22.888140123424854 27.03469201717076 28.107390844641934 21.96977701476245 54-55 22.328654925590218 27.125777781340243 28.359425154098894 22.186142138970645 56-57 22.55782670824064 27.15217443579656 28.14673989476315 22.14325896119965 58-59 22.728138870084738 26.883325694273914 27.993442553665908 22.39509288197544 60-61 22.15850671812162 27.242750119686693 28.476561446820853 22.122181715370832 62-63 22.18887471189573 27.21799300898543 28.52900862982964 22.0641236492892 64-65 21.857450050388643 27.450735353591295 28.215236389418603 22.47657820660146 66-67 21.957357830895987 27.164316522228305 28.157556471598973 22.72076917527674 68-69 22.43751538216727 26.91649279845868 28.273912700519244 22.372079118854806 70-71 22.495505828395878 27.049120002215204 27.77782433272391 22.677549836665005 72-73 22.411907314041127 27.001136021650723 28.02002137965057 22.566935284657585 74-75 22.215745012325723 27.693952561076646 27.64263484154356 22.447667585054067 76-77 22.32328086550422 27.069559647694835 27.9693594776195 22.637800009181444 78-79 22.410905370635263 27.35451235232265 27.916183608497352 22.318398668544734 80-81 22.719989157119027 27.224976663759197 27.641021212843274 22.41401296627849 82-83 22.576945267111643 27.303685251506625 27.874071892034973 22.24529758934676 84-85 22.465098823662295 27.59667849016318 27.707425848094484 22.23079683808004 86-87 22.689584338323915 27.483116158208226 27.40332415664591 22.423975346821948 88-89 22.458456230823025 27.72565545783266 27.02059686326075 22.795291448083567 90-91 22.51449922892431 27.36313975826572 27.302145986079644 22.82021502673032 92-93 22.683321121701812 27.218651843166946 27.527233881466167 22.570793153665075 94-95 22.459620277677026 27.087036352543763 27.5267077366679 22.92663563311131 96-97 22.43891775301859 26.80628930359316 27.770215618692294 22.984577324695955 98-99 22.4145491165304 27.137554120972656 27.11381713191226 23.334079630584682 100-101 22.745508542413663 26.54249309279789 27.35401419296589 23.35798417182255 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 757.0 1 503.0 2 350.5 3 463.0 4 380.0 5 356.0 6 734.5 7 1769.0 8 2925.5 9 3412.0 10 2968.0 11 2320.0 12 2303.5 13 2582.0 14 2870.5 15 3158.5 16 3443.5 17 3778.0 18 4102.5 19 4539.0 20 5201.0 21 6146.0 22 7562.0 23 9394.0 24 11674.0 25 14595.0 26 18516.5 27 23339.5 28 27449.5 29 31174.5 30 35504.0 31 41636.5 32 49646.5 33 53121.5 34 53598.5 35 56387.0 36 60655.0 37 65228.0 38 69516.5 39 73121.5 40 77101.5 41 82427.0 42 86231.5 43 89042.0 44 92122.0 45 96179.0 46 98674.0 47 104881.0 48 112488.0 49 109002.0 50 100397.0 51 94476.0 52 91348.5 53 90728.0 54 91158.5 55 85939.5 56 77194.5 57 70447.0 58 65292.5 59 58038.5 60 47330.5 61 39102.0 62 33151.0 63 25306.0 64 17794.0 65 13061.0 66 9794.0 67 7385.0 68 5662.0 69 4457.0 70 3418.5 71 2428.0 72 1676.5 73 1255.0 74 934.5 75 647.0 76 469.0 77 323.5 78 202.5 79 123.0 80 74.0 81 42.5 82 28.0 83 11.5 84 7.0 85 5.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.008051981552582355 3 0.005501580155836812 4 0.0 5 4.73645973681315E-4 6 2.9147444534234764E-4 7 0.0 8 7.286861133558691E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001675978060718499 26-27 0.019528787837937295 28-29 2.368229868406575E-4 30-31 1.0930291700338037E-4 32-33 0.0031515674402641343 34-35 0.013626430319754754 36-37 0.006503523561701132 38-39 0.018526844432072975 40-41 0.020694685619306683 42-43 0.0161586145636664 44-45 0.008416324609260289 46-47 0.0016577609078846024 48-49 2.0038868117286404E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.3721166801352146E-4 68-69 3.8256020951183133E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.9147444534234764E-4 82-83 0.0013298521568744612 84-85 0.004772894042480943 86-87 0.0010930291700338038 88-89 1.8217152833896728E-5 90-91 0.004499636749972492 92-93 5.465145850169019E-4 94-95 9.108576416948365E-5 96-97 2.1860583400676073E-4 98-99 1.6395437550507057E-4 100-101 0.002441098479742162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2744666.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.317435700784266 #Duplication Level Percentage of deduplicated Percentage of total 1 74.27859822164376 38.860657864052364 2 14.346647743135993 15.011596416466377 3 4.91848524697351 7.719676069613778 4 2.2021088532867483 4.608347533518289 5 1.2046062456410096 3.151095490054332 6 0.7117931874714233 2.234351659067548 7 0.4565030325723972 1.671814763767359 8 0.31045900048441977 1.2993935036458706 9 0.22920415198638125 1.0792236135510256 >10 1.205306134261165 11.385979784638916 >50 0.08630870238087492 3.086483248347293 >100 0.04293448438784701 4.288512231449965 >500 0.0032783643702932763 1.2052438909515504 >1k 0.0034876216704629722 3.155595413964813 >5k 2.0925730022777834E-4 0.8344052310609844 >10k+ 6.975243340925944E-5 0.40762328584960406 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11170 0.40697119430925294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9712 0.35384997664561 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6904 0.25154244633044603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6249 0.2276779761180413 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3233 0.11779211022397625 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3124 0.11382077090618677 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3107 0.11320138770983428 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3107 0.11320138770983428 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2989 0.10890213964103465 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2905 0.10584165796494001 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8217152833896728E-5 0.0 12-13 0.0 0.0 0.0 7.286861133558691E-5 0.0 14-15 0.0 0.0 0.0 1.0930291700338038E-4 0.0 16-17 0.0 0.0 0.0 1.0930291700338038E-4 0.0 18-19 0.0 0.0 0.0 1.0930291700338038E-4 0.0 20-21 0.0 0.0 0.0 1.4573722267117382E-4 0.0 22-23 0.0 0.0 0.0 1.821715283389673E-4 0.0 24-25 0.0 0.0 0.0 3.643430566779346E-4 0.0 26-27 0.0 0.0 0.0 7.833375718575594E-4 0.0 28-29 0.0 0.0 0.0 0.0023864470212404715 0.0 30-31 0.0 0.0 0.0 0.007778724260073903 0.0 32-33 0.0 0.0 0.0 0.019346616309598327 0.0 34-35 0.0 0.0 0.0 0.03701725455847815 3.6434305667793455E-5 36-37 0.0 0.0 0.0 0.06498058415850963 3.6434305667793455E-5 38-39 0.0 3.6434305667793455E-5 0.0 0.10662499553679755 3.6434305667793455E-5 40-41 0.0 3.6434305667793455E-5 0.0 0.15149384296668522 3.6434305667793455E-5 42-43 0.0 3.6434305667793455E-5 0.0 0.19964177790667426 3.6434305667793455E-5 44-45 0.0 3.6434305667793455E-5 0.0 0.2606145884417266 3.6434305667793455E-5 46-47 0.0 3.6434305667793455E-5 0.0 0.3321351304676052 3.6434305667793455E-5 48-49 0.0 3.6434305667793455E-5 0.0 0.4071169315319241 3.6434305667793455E-5 50-51 0.0 3.6434305667793455E-5 0.0 0.48699914670856126 7.286861133558691E-5 52-53 0.0 3.6434305667793455E-5 0.0 0.5660980243133409 7.286861133558691E-5 54-55 0.0 3.6434305667793455E-5 0.0 0.6517732940911571 7.286861133558691E-5 56-57 0.0 3.6434305667793455E-5 0.0 0.7566130086502328 7.286861133558691E-5 58-59 0.0 3.6434305667793455E-5 0.0 0.8662074000989556 7.286861133558691E-5 60-61 0.0 3.6434305667793455E-5 0.0 0.9812305030921795 7.286861133558691E-5 62-63 0.0 3.6434305667793455E-5 0.0 1.096180737474068 7.286861133558691E-5 64-65 0.0 3.6434305667793455E-5 0.0 1.2176891468761593 7.286861133558691E-5 66-67 0.0 3.6434305667793455E-5 0.0 1.3554108223004184 7.286861133558691E-5 68-69 0.0 3.6434305667793455E-5 0.0 1.5078337400616322 7.286861133558691E-5 70-71 0.0 3.6434305667793455E-5 0.0 1.6649566832539917 7.286861133558691E-5 72-73 0.0 3.6434305667793455E-5 0.0 1.8288564073005604 7.286861133558691E-5 74-75 0.0 3.6434305667793455E-5 0.0 1.9936487718359903 7.286861133558691E-5 76-77 0.0 3.6434305667793455E-5 0.0 2.17297842433287 7.286861133558691E-5 78-79 0.0 3.6434305667793455E-5 0.0 2.37994349767877 7.286861133558691E-5 80-81 0.0 3.6434305667793455E-5 0.0 2.5954159813981006 1.0930291700338037E-4 82-83 0.0 3.6434305667793455E-5 0.0 2.8132931292915058 1.4573722267117382E-4 84-85 0.0 3.6434305667793455E-5 0.0 3.03794705803912 1.4573722267117382E-4 86-87 0.0 3.6434305667793455E-5 0.0 3.2689952074314323 1.4573722267117382E-4 88-89 0.0 3.6434305667793455E-5 0.0 3.510955431371249 1.4573722267117382E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17980 0.0 24.069336 1 TAAGGTG 1715 0.0 23.817194 5 GAGTACT 11210 0.0 22.28622 12-13 GGTATCA 13645 0.0 22.246555 1 GTACTTT 11625 0.0 21.34763 14-15 GTAAGGT 1760 0.0 21.319191 4 AGAGTAC 15120 0.0 21.156418 10-11 GTACATG 8395 0.0 21.106855 1 AGTACTT 11600 0.0 20.574741 12-13 ATCAACG 20885 0.0 20.537573 3 TCAACGC 21015 0.0 20.453869 4 TATCAAC 20975 0.0 20.451315 2 CAACGCA 21155 0.0 20.340961 5 TACATGG 8195 0.0 20.230745 2 ATACCGA 190 8.264236E-6 19.998281 6 AACGCAG 21605 0.0 19.873322 6 ACATGGG 8095 0.0 19.246548 3 CGTAGCG 125 0.006033048 19.0001 3 GGTAAGG 1955 0.0 18.708536 3 AGGTAAG 1850 0.0 18.488266 2 >>END_MODULE