FastQCFastQC Report
Thu 26 May 2016
SRR937953_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937953_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735164
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118620.43368514648481776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93110.34041834420166395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61220.22382570112797623No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59360.21702537763731902No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39560.1446348372529033No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36350.13289879509967228No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32870.12017560921392648No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32130.11747010416925639No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30740.11238814199075449No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29150.10657496223261201No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA29020.10609967080584565No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27950.10218765675476862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA163850.025.5889031
GGTATCA122750.022.576881
GAGTACT105900.022.4224612-13
TATCAAC190750.021.8974322
GTACATG84150.021.8230721
ATCAACG191600.021.7258833
TACATGG80900.021.6743812
CAACGCA192150.021.6636965
TCAACGC192700.021.6018644
GTAAGGT16100.021.545964
AGAGTAC142750.021.38053510-11
ACATGGG78600.021.3413073
AACGCAG196100.021.2515626
GTACTTT111500.021.1120514-15
TAAGGTG17900.020.9721475
AGTACTT109400.020.87948612-13
ACGCAGA215400.019.290057
CGCAGAG216150.019.200778
AGGTAAG16000.019.0076682
GTGTAAG12950.018.7849371
Produced by FastQC (version 0.11.5)