##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937953_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2735164 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.377238074206886 33.0 31.0 34.0 31.0 34.0 2 32.587725635464636 34.0 31.0 34.0 31.0 34.0 3 32.668593181249825 34.0 31.0 34.0 31.0 34.0 4 36.15706919219469 37.0 37.0 37.0 35.0 37.0 5 36.17930003465971 37.0 37.0 37.0 35.0 37.0 6 36.220712907891446 37.0 37.0 37.0 35.0 37.0 7 36.18947785215073 37.0 37.0 37.0 35.0 37.0 8 36.17727419635532 37.0 37.0 37.0 35.0 37.0 9 37.92950806606112 39.0 38.0 39.0 35.0 39.0 10-11 37.86635079285922 39.0 38.0 39.0 35.0 39.0 12-13 37.795798167861236 39.0 38.0 39.0 35.0 39.0 14-15 39.16152395249426 40.5 39.0 41.0 36.0 41.0 16-17 39.09677006570721 40.0 39.0 41.0 36.0 41.0 18-19 39.04322556161166 40.0 39.0 41.0 36.0 41.0 20-21 38.94095088996492 40.0 39.0 41.0 36.0 41.0 22-23 38.80634378779481 40.0 38.0 41.0 35.5 41.0 24-25 38.77255641709236 40.0 38.5 41.0 35.0 41.0 26-27 38.79531465023669 40.0 38.0 41.0 35.0 41.0 28-29 38.72107120450546 40.0 38.0 41.0 35.0 41.0 30-31 38.551837293851484 40.0 38.0 41.0 35.0 41.0 32-33 38.33698948216633 40.0 38.0 41.0 34.5 41.0 34-35 38.147448379694964 40.0 38.0 41.0 34.0 41.0 36-37 37.93965535521819 40.0 38.0 41.0 34.0 41.0 38-39 37.70964794067193 40.0 37.5 41.0 33.0 41.0 40-41 37.50569910981572 40.0 37.0 41.0 33.0 41.0 42-43 37.229512928658025 40.0 37.0 41.0 32.5 41.0 44-45 37.017670055616406 40.0 37.0 41.0 32.0 41.0 46-47 36.87819615204061 40.0 37.0 41.0 31.0 41.0 48-49 36.62411705477258 40.0 36.5 41.0 31.0 41.0 50-51 35.98355162615478 39.0 35.5 40.5 30.0 41.0 52-53 36.0623598804313 39.0 36.0 40.0 30.0 41.0 54-55 36.206033532175766 39.0 36.0 41.0 30.0 41.0 56-57 35.99988190836089 39.0 35.0 41.0 29.5 41.0 58-59 35.74634336368861 39.0 35.0 41.0 29.0 41.0 60-61 35.37660100089062 38.5 35.0 40.0 28.0 41.0 62-63 34.98187019133039 38.0 35.0 40.0 27.5 41.0 64-65 34.574486027163275 37.0 34.0 40.0 26.5 41.0 66-67 34.15117393326324 37.0 34.0 39.5 26.0 41.0 68-69 33.678022231939295 36.0 34.0 39.0 25.5 41.0 70-71 33.19778283861589 35.5 33.0 39.0 24.5 40.0 72-73 32.72578408461065 35.0 33.0 38.0 23.0 40.0 74-75 32.217773230416896 35.0 32.0 37.0 21.5 39.5 76-77 31.701597417924482 35.0 32.0 37.0 19.5 39.0 78-79 31.15098345108374 35.0 31.0 36.0 18.0 39.0 80-81 30.635947972406775 34.5 31.0 36.0 13.0 37.5 82-83 30.114301189983486 34.0 30.0 35.0 7.0 37.0 84-85 29.642790158103864 34.0 30.0 35.0 4.5 36.5 86-87 29.182135513629163 34.0 29.0 35.0 2.0 36.0 88-89 28.645595108739364 34.0 29.0 35.0 2.0 36.0 90-91 28.06327536484101 33.5 27.5 35.0 2.0 35.0 92-93 27.483965678109247 33.0 26.5 35.0 2.0 35.0 94-95 26.91021653546186 33.0 25.5 35.0 2.0 35.0 96-97 26.102017831471898 32.5 24.5 35.0 2.0 35.0 98-99 25.04634676385036 32.0 19.0 34.0 2.0 35.0 100-101 22.00181872092496 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30491372921510873 1101 2 0.19153298052233225 1101 3 0.18940086280044 1101 4 0.04235151962321737 1101 5 0.037291654236483396 1101 6 0.09147462407721463 1101 7 0.05780957131693043 1101 8 0.07377427366891709 1101 9 0.08517093668007192 1101 10-11 0.08820071360479886 1101 12-13 0.026066563434454793 1101 14-15 0.07607432755917642 1101 16-17 0.2180690629024511 1101 18-19 0.21115023821980827 1101 20-21 0.21594449198357069 1101 22-23 0.30740623538855516 1101 24-25 0.3063223993976081 1101 26-27 0.22645062551869444 1101 28-29 0.26006341219769524 1101 30-31 0.25875083818711886 1101 32-33 0.2129295855647655 1101 34-35 0.2217378380746169 1101 36-37 0.31117103917483746 1101 38-39 0.22894479647913357 1101 40-41 0.22681150590156562 1101 42-43 0.45013042457866703 1101 44-45 0.4914568046790748 1101 46-47 0.49898454694474026 1101 48-49 0.513367537936837 1101 50-51 0.5483954059020917 1101 52-53 0.6326385287848808 1101 54-55 0.650046829135654 1101 56-57 0.658470200833527 1101 58-59 0.6410722525721724 1101 60-61 0.5467905940960875 1101 62-63 0.5949124328696556 1101 64-65 0.56825690831554 1101 66-67 0.47547308793865994 1101 68-69 0.5682541032160628 1101 70-71 0.7040756014963208 1101 72-73 0.7320779465809721 1101 74-75 0.6564700537874373 1101 76-77 0.5807373607181994 1101 78-79 0.6700356009572523 1101 80-81 0.8536718424156255 1101 82-83 0.6557651095515062 1101 84-85 0.6725679281674282 1101 86-87 0.6562569748676985 1101 88-89 0.8166630090341869 1101 90-91 0.8107313338441067 1101 92-93 0.9735949685664842 1101 94-95 1.068331209312209 1101 96-97 1.0744403074254087 1101 98-99 1.2615347013843703 1101 100-101 1.248833023464698 1102 1 0.3308464912169313 1102 2 0.2778693838436581 1102 3 0.21542515508039628 1102 4 0.20031620335007005 1102 5 0.14043143106116673 1102 6 0.23920881726664334 1102 7 0.058329780409565046 1102 8 0.14770854051499782 1102 9 0.19947337145754318 1102 10-11 0.2500971633110751 1102 12-13 0.15813019643696435 1102 14-15 0.2826753903193975 1102 16-17 0.2661891967528689 1102 18-19 0.30761825054973 1102 20-21 0.31079926514343725 1102 22-23 0.3751590373321747 1102 24-25 0.3681384177728546 1102 26-27 0.2979434122841411 1102 28-29 0.29370619474698145 1102 30-31 0.3312214133648226 1102 32-33 0.3454624013316092 1102 34-35 0.3954043608393505 1102 36-37 0.41105882449525666 1102 38-39 0.36442640069500243 1102 40-41 0.47737939001702756 1102 42-43 0.5931739432620091 1102 44-45 0.4740950244079585 1102 46-47 0.5447013983896554 1102 48-49 0.5858556263882306 1102 50-51 0.6619785712497332 1102 52-53 0.5708355552760551 1102 54-55 0.5939136878957854 1102 56-57 0.5698687474480195 1102 58-59 0.5176475643336715 1102 60-61 0.49954843020500306 1102 62-63 0.5733125124361322 1102 64-65 0.5939178181841669 1102 66-67 0.5232288303825499 1102 68-69 0.5165879278339602 1102 70-71 0.5319933453254109 1102 72-73 0.6975573382145299 1102 74-75 0.6485239283650159 1102 76-77 0.5812587950192842 1102 78-79 0.6541535121354052 1102 80-81 0.7292158890196667 1102 82-83 0.8212522127911761 1102 84-85 0.8696199325003384 1102 86-87 0.884721522019845 1102 88-89 0.9220953670775707 1102 90-91 1.0144811924046024 1102 92-93 1.101009367936868 1102 94-95 1.1255613445391823 1102 96-97 1.2635802759591748 1102 98-99 1.4553964791329967 1102 100-101 1.3790359251907311 1103 1 0.2607338490084814 1103 2 0.17870994864433953 1103 3 0.1961299966753387 1103 4 0.09066043299662141 1103 5 0.10836835086909957 1103 6 0.04110999727829068 1103 7 0.13540976005663907 1103 8 0.15777893582132663 1103 9 0.10506466495828448 1103 10-11 0.12410386478319424 1103 12-13 0.041075595474431736 1103 14-15 0.19441553091564856 1103 16-17 0.197639398373191 1103 18-19 0.15921618443604046 1103 20-21 0.1547248672798034 1103 22-23 0.12305825007926785 1103 24-25 0.17475110614329736 1103 26-27 0.12092611576655798 1103 28-29 0.20948117133625743 1103 30-31 0.17746212630039082 1103 32-33 0.1945904949661852 1103 34-35 0.21270157258546618 1103 36-37 0.2745928618510618 1103 38-39 0.33284389116237634 1103 40-41 0.29563635475134475 1103 42-43 0.35777262198771353 1103 44-45 0.4423622974974606 1103 46-47 0.41897757484371567 1103 48-49 0.3166930188533499 1103 50-51 0.3631730909532678 1103 52-53 0.37611697337252536 1103 54-55 0.32808189387407793 1103 56-57 0.25591165103053726 1103 58-59 0.3385563100532778 1103 60-61 0.28436599314323274 1103 62-63 0.3454168538155358 1103 64-65 0.28222259008490624 1103 66-67 0.3602031302994604 1103 68-69 0.3509425172039329 1103 70-71 0.533033413242542 1103 72-73 0.48446413055288673 1103 74-75 0.3864680466674528 1103 76-77 0.4193177282676537 1103 78-79 0.3704958300506327 1103 80-81 0.43231714205751004 1103 82-83 0.5353107961802159 1103 84-85 0.572508770946552 1103 86-87 0.6755130023898914 1103 88-89 0.6387159758744545 1103 90-91 0.701327800608663 1103 92-93 0.7844221484924638 1103 94-95 0.9465046596923266 1103 96-97 0.8429760605707806 1103 98-99 0.9994481416936765 1103 100-101 0.9240373447588333 1104 1 0.2507113668865557 1104 2 0.2049749666220677 1104 3 0.13919641460363863 1104 4 0.13263981641246403 1104 5 0.15685080763885395 1104 6 0.1326608650722534 1104 7 0.09650173744695678 1104 8 0.16875770536363177 1104 9 0.1262524704002317 1104 10-11 0.24539551052234998 1104 12-13 0.09724143699938992 1104 14-15 0.160206829856115 1104 16-17 0.2501857777151528 1104 18-19 0.27586650771619503 1104 20-21 0.31524582269026524 1104 22-23 0.28390966079255264 1104 24-25 0.41022960861074864 1104 26-27 0.3970878913758469 1104 28-29 0.4500167560707311 1104 30-31 0.35956173326187013 1104 32-33 0.28584578663583926 1104 34-35 0.28507566871646617 1104 36-37 0.3204400472010178 1104 38-39 0.33409814642215707 1104 40-41 0.2649588217257488 1104 42-43 0.2515338331522443 1104 44-45 0.30012102127827944 1104 46-47 0.4911242587307285 1104 48-49 0.5997654559313119 1104 50-51 0.6112112866433961 1104 52-53 0.5673784436395195 1104 54-55 0.5167435698285701 1104 56-57 0.4494023990792968 1104 58-59 0.40288706830668275 1104 60-61 0.5197404560038805 1104 62-63 0.4695201599630394 1104 64-65 0.5347195406481617 1104 66-67 0.5095950367355613 1104 68-69 0.5956327103747867 1104 70-71 0.629335024461291 1104 72-73 0.550206997262606 1104 74-75 0.5541334536974958 1104 76-77 0.5223213238126867 1104 78-79 0.569132552444259 1104 80-81 0.6890793178804593 1104 82-83 0.7074507035411166 1104 84-85 0.55911476777354 1104 86-87 0.6395559575930676 1104 88-89 0.7141889375352584 1104 90-91 0.6762767773532197 1104 92-93 0.7635269295309435 1104 94-95 0.92213288352497 1104 96-97 0.9498036099397638 1104 98-99 1.0056392955367635 1104 100-101 0.9962436222137292 1105 1 0.2381444682437035 1105 2 0.18714293929636483 1105 3 0.08897448463487478 1105 4 0.10046453740174144 1105 5 0.07440793405673674 1105 6 0.05431410634574263 1105 7 0.05992895720329017 1105 8 0.09227862888455718 1105 9 0.12013798010852383 1105 10-11 0.13254229482103597 1105 12-13 0.06188105195546001 1105 14-15 0.22759636718235754 1105 16-17 0.15395219413979788 1105 18-19 0.21389735872173077 1105 20-21 0.24749553500326726 1105 22-23 0.26850470709646146 1105 24-25 0.29081438951198635 1105 26-27 0.12676257674673508 1105 28-29 0.1632042366632831 1105 30-31 0.1916849546255932 1105 32-33 0.20804015345046167 1105 34-35 0.2743216547978946 1105 36-37 0.23988083847724795 1105 38-39 0.34362378253007364 1105 40-41 0.3747662084318648 1105 42-43 0.2568354935171939 1105 44-45 0.3511991625463011 1105 46-47 0.43704138660987724 1105 48-49 0.4134082126120404 1105 50-51 0.37204978007682854 1105 52-53 0.3810600152324781 1105 54-55 0.5644653628035243 1105 56-57 0.5777678802928818 1105 58-59 0.4849303766158144 1105 60-61 0.5179823688027625 1105 62-63 0.5047572505798783 1105 64-65 0.5383042119543475 1105 66-67 0.6258023174998044 1105 68-69 0.70092386788113 1105 70-71 0.6702801904064515 1105 72-73 0.7023797190555783 1105 74-75 0.5830322974697566 1105 76-77 0.6208244381385803 1105 78-79 0.5701257575513878 1105 80-81 0.5999465800135475 1105 82-83 0.7784614438556581 1105 84-85 0.74196040315082 1105 86-87 0.8884463077492377 1105 88-89 0.7937343920807152 1105 90-91 0.7268389086115548 1105 92-93 0.80154727767788 1105 94-95 0.9920202746148945 1105 96-97 0.979855484928347 1105 98-99 0.9758705410844648 1105 100-101 0.6205951848564304 1106 1 0.024842578915851732 1106 2 0.0077754923525290565 1106 3 -0.009821057662243504 1106 4 0.056160739933389436 1106 5 0.05764675620550008 1106 6 0.11767019403062307 1106 7 0.1277261747223477 1106 8 0.023630362784061276 1106 9 0.12473364244828389 1106 10-11 -0.23402448561738964 1106 12-13 -0.11146298667318888 1106 14-15 0.009328161461525042 1106 16-17 0.09846382663014452 1106 18-19 0.20268703326283344 1106 20-21 0.11394137695906892 1106 22-23 0.0678856812335411 1106 24-25 0.1019891053065507 1106 26-27 0.096980355599527 1106 28-29 -0.07754732881819137 1106 30-31 0.06043534655587024 1106 32-33 0.0360813083681748 1106 34-35 -0.12281120732327366 1106 36-37 -0.16317912909332222 1106 38-39 -0.14146159108025103 1106 40-41 0.09691004460054131 1106 42-43 -5.68629141305621E-4 1106 44-45 0.04882048176117593 1106 46-47 0.22060629474142246 1106 48-49 0.17310232783506052 1106 50-51 0.18259964704449771 1106 52-53 0.16847815779655662 1106 54-55 -0.030349287773844935 1106 56-57 0.01599071168671884 1106 58-59 0.16372336576718283 1106 60-61 -0.08738976338748472 1106 62-63 0.06480880620709684 1106 64-65 0.08740747550169914 1106 66-67 0.03228439620836099 1106 68-69 -0.13819211660290875 1106 70-71 0.06545004178649805 1106 72-73 0.08641875083104367 1106 74-75 0.25601464045129063 1106 76-77 0.2697937735144329 1106 78-79 0.34018474865569104 1106 80-81 0.34836094213023827 1106 82-83 0.4253703458238185 1106 84-85 0.39395544019383166 1106 86-87 0.18710836979515832 1106 88-89 0.33009320312105217 1106 90-91 0.19794133006872983 1106 92-93 0.2757694640664745 1106 94-95 0.4686455534452847 1106 96-97 0.49221360617141485 1106 98-99 0.35825898653224897 1106 100-101 0.08439538552472925 1107 1 0.14075071084216972 1107 2 0.062401844643133586 1107 3 0.04660731340548807 1107 4 0.005106647353301241 1107 5 -0.03138926648913554 1107 6 -0.05576969304095769 1107 7 -0.04510897759386978 1107 8 0.0042375155227105665 1107 9 0.0011398120330170514 1107 10-11 0.01571603514739195 1107 12-13 -0.08766711607256639 1107 14-15 -0.008402919611157245 1107 16-17 0.021526560872281664 1107 18-19 0.044216302568351296 1107 20-21 0.11500318701548196 1107 22-23 0.15906336334328586 1107 24-25 0.1692596943626441 1107 26-27 0.19331703922551924 1107 28-29 0.17526333783317938 1107 30-31 0.24499998770755127 1107 32-33 0.13753171605640802 1107 34-35 0.22115002275165097 1107 36-37 0.21687838641450696 1107 38-39 0.22696929545468691 1107 40-41 0.2761117564944797 1107 42-43 0.3641960977388834 1107 44-45 0.2704281730506537 1107 46-47 0.28127533542805594 1107 48-49 0.1634517789862997 1107 50-51 0.12125371439346111 1107 52-53 -0.06645579924266798 1107 54-55 -3.9974525532926464E-4 1107 56-57 0.024663912642637342 1107 58-59 0.08588055092879188 1107 60-61 -0.050322939599688254 1107 62-63 0.04541536161786297 1107 64-65 0.06164866599051777 1107 66-67 0.13180866038403138 1107 68-69 0.13663414460714307 1107 70-71 0.09592164888045573 1107 72-73 0.15896446194511782 1107 74-75 0.11187774819060792 1107 76-77 0.2181403221299938 1107 78-79 0.0728447978850646 1107 80-81 0.18177491472865626 1107 82-83 0.19136905655129866 1107 84-85 0.26672818369215534 1107 86-87 0.2924105866462092 1107 88-89 0.29927681836925757 1107 90-91 0.28491561581988023 1107 92-93 0.41007147209169403 1107 94-95 0.4052305964505045 1107 96-97 0.34079707892108146 1107 98-99 0.42873065574831415 1107 100-101 0.6206978685450864 1108 1 0.2199770019289673 1108 2 0.1409005827061378 1108 3 0.13436002352887044 1108 4 0.09539218632983903 1108 5 0.09225648589675473 1108 6 0.10235609501550158 1108 7 0.07403120266157259 1108 8 0.09111125413812715 1108 9 0.2065188664606339 1108 10-11 0.15053413409879113 1108 12-13 0.012197677222594905 1108 14-15 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-1.1339534439963117 2316 100-101 -1.2518381157248086 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5484.0 3 1050.0 4 986.0 5 1073.0 6 1190.0 7 1444.0 8 1788.0 9 2208.0 10 3028.0 11 4075.0 12 5883.0 13 8087.0 14 10311.0 15 13442.0 16 17515.0 17 24899.0 18 21815.0 19 16077.0 20 14249.0 21 14341.0 22 15318.0 23 16181.0 24 18429.0 25 20646.0 26 23500.0 27 28514.0 28 34117.0 29 42478.0 30 52610.0 31 68123.0 32 89287.0 33 123128.0 34 178852.0 35 276932.0 36 435271.0 37 611833.0 38 472981.0 39 58013.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49605843426751 18.615803946817973 14.790815856175366 23.097321762739156 2 15.311575500601334 19.764754681204078 40.666195546893974 24.257474271300612 3 19.66994055784179 23.513927754915407 29.735052583447647 27.08107910379515 4 12.621543916411223 16.6702701358014 37.47055490132165 33.23763104646573 5 13.646985819560056 37.1817523565464 34.40544928142851 14.765812542465035 6 32.04241305886546 36.27054355090058 17.315103467105068 14.371939923128894 7 28.409057233871227 32.803844689022064 20.351117629820635 18.435980447286074 8 26.238863470427198 34.46365420097183 19.568321849911257 19.729160478689707 9 25.767117790855853 17.207902299954238 20.50261226254528 36.522367646644625 10-11 24.770861722369087 26.412088703125736 27.291103802296877 21.5259457722083 12-13 26.149620376859918 23.777822103234804 27.515604899142303 22.556952620762978 14-15 22.62081281583294 25.342867756247067 26.290176821636706 25.74614260628329 16-17 21.897889522677946 28.455649174893466 27.082721360877976 22.563739941550608 18-19 21.857493720876978 27.018921871101842 29.409908759751573 21.71367564826961 20-21 22.354720358138273 25.967439447455515 29.774744022309008 21.903096172097207 22-23 21.993431940800402 25.931816419196846 29.779676989331023 22.29507465067173 24-25 22.069720450756506 26.08389551043827 29.73774785679889 22.108636182006332 26-27 21.574860675383476 26.34137498904774 29.79555723352995 22.288207102038832 28-29 21.59275303970313 27.143212267137184 29.453388474892257 21.810646218267433 30-31 21.820276830673485 26.345834744157447 29.309763561426777 22.524124863742287 32-33 21.510817174234003 26.35376340565149 29.49428999323349 22.64112942688102 34-35 22.400851135756223 26.492972386015527 29.179204979788086 21.926971498440157 36-37 22.396304546733333 25.906696134100482 29.41646578837519 22.280533530790994 38-39 21.766865820832937 26.590802650845518 29.215753435304215 22.426578093017326 40-41 22.419497072271675 26.389386221297418 29.139080720006465 22.05203598642444 42-43 22.020694309065007 26.40428150551281 28.875565317098772 22.699458868323404 44-45 22.029193733114706 26.593946233271947 29.04155519507589 22.335304838537454 46-47 22.60826427723642 26.994788496255985 28.345288591065454 22.051658635442138 48-49 22.22781348403897 26.99241577435553 28.323719187083356 22.456051554522148 50-51 22.12762588341605 27.20393917025311 28.5429118358506 22.125523110480238 52-53 22.687222575320185 27.00296139074926 28.0963318688504 22.213484165080164 54-55 22.09615375473069 27.063923435667537 28.419180285364583 22.42074252423719 56-57 22.334894974139857 27.13563309352255 28.14767653666077 22.381795395676825 58-59 22.47005005659868 26.772664931985595 28.085962705027363 22.671322306388365 60-61 22.00431606687161 27.079680840715113 28.54197614570328 22.374026946709996 62-63 21.96631096326247 27.088304681970516 28.704977659671137 22.240406695095878 64-65 21.660676486576076 27.380020108867264 28.34251409110864 22.61678931344802 66-67 21.773686931809454 27.07049712993157 28.287871995459962 22.867943942799013 68-69 22.243392550730018 26.7868566815891 28.46446453401153 22.505286233669356 70-71 22.25214687597953 26.929518388026143 27.941793409066058 22.876541326928272 72-73 22.13808803739169 26.919430083602442 28.240899820257585 22.701582058748286 74-75 22.025218961197645 27.65045130775109 27.898500968152547 22.42582876289872 76-77 22.197329136228607 27.039913025146245 27.96038226662305 22.8023755720021 78-79 22.334644037502706 27.299943926214432 27.90251315503442 22.462898881248446 80-81 22.584433350117433 27.26112732842828 27.55817665430348 22.59626266715081 82-83 22.35698262254551 27.331976282510844 27.865069435035718 22.445971659907922 84-85 22.338844783916922 27.5910469903377 27.72468136821564 22.345426857529738 86-87 22.510433600471533 27.526514544881486 27.433147806475816 22.52990404817117 88-89 22.39317667936801 27.630487466780586 27.113972672483094 22.862363181368305 90-91 22.359577749721403 27.43686721122372 27.293658984816453 22.909896054238423 92-93 22.643872857960258 27.152956691897558 27.588500887162088 22.614669562980097 94-95 22.364464951621112 27.00544720864164 27.710787843450046 22.919299996287197 96-97 22.410390606012005 26.71130501986707 27.918398608070493 22.959905766050433 98-99 22.38929443948681 27.01839241043346 27.251653086004357 23.34066006407537 100-101 22.637592497042036 26.3349840319354 27.59679254479426 23.43063092622831 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 730.0 1 495.5 2 375.5 3 491.5 4 404.0 5 407.0 6 767.5 7 1729.5 8 2997.5 9 3602.0 10 3142.0 11 2535.5 12 2511.0 13 2670.5 14 2824.5 15 3072.0 16 3307.5 17 3518.0 18 3844.0 19 4285.5 20 4880.5 21 5787.0 22 7167.0 23 8911.5 24 11088.0 25 13954.5 26 17998.0 27 23006.5 28 26901.5 29 30189.5 30 34142.0 31 40303.5 32 48792.5 33 52654.0 34 52868.5 35 55527.5 36 60356.5 37 65133.5 38 68775.5 39 72261.0 40 76861.5 41 81968.0 42 85148.5 43 87980.0 44 91017.5 45 95587.5 46 98242.5 47 103847.0 48 112891.5 49 109522.5 50 99778.5 51 94471.5 52 92019.0 53 91853.5 54 92809.5 55 87198.0 56 78164.0 57 71357.0 58 67094.5 59 59932.5 60 47655.0 61 39441.5 62 33433.0 63 24711.0 64 17169.0 65 13074.5 66 9805.5 67 7195.0 68 5458.0 69 4249.5 70 3193.0 71 2179.0 72 1553.5 73 1280.5 74 962.0 75 655.5 76 464.5 77 313.0 78 198.0 79 122.0 80 76.0 81 50.5 82 37.5 83 22.5 84 14.0 85 14.0 86 12.0 87 7.5 88 7.0 89 6.0 90 3.0 91 2.5 92 2.0 93 2.5 94 3.0 95 1.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4575594004600821 2 0.04577422048549923 3 0.05169708288058778 4 0.06167820284268146 5 0.0743282669704632 6 0.1022973393917147 7 0.06708921293202164 8 0.05001528244741449 9 0.04851628640915133 10-11 0.07072702039073342 12-13 0.10416194421979814 14-15 0.05193472859397096 16-17 0.05546285341573668 18-19 0.05491444023100625 20-21 0.07012376588752997 22-23 0.05966735449866992 24-25 0.08540621330201771 26-27 0.06250082261977709 28-29 0.06268362701468724 30-31 0.06699781073456655 32-33 0.067582784798279 34-35 0.06855164809130275 36-37 0.06401809909753126 38-39 0.037200694364213624 40-41 0.041423475886637875 42-43 0.039449188421608355 44-45 0.01959663113436708 46-47 0.02678084385433561 48-49 0.027000209128227776 50-51 0.02484311726828812 52-53 0.023325840790533948 54-55 0.02292367112173164 56-57 0.023654888701372202 58-59 0.020455811790444742 60-61 0.026689441656880537 62-63 0.019578350694876064 64-65 0.02009020300062446 66-67 0.01411249928706286 68-69 0.017055650045116125 70-71 0.039302944905680245 72-73 0.035592015689004386 74-75 0.03043693175253842 76-77 0.021223590249067333 78-79 0.013692049178769537 80-81 0.015830860599218184 82-83 0.020382690032480683 84-85 0.01694596740817004 86-87 0.009140219745507034 88-89 0.039120140510770104 90-91 0.050545415192653896 92-93 0.032301536580621855 94-95 0.05039917167672578 96-97 0.040856782262416444 98-99 0.03231981702011287 100-101 0.16090442839990582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2735164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.08069417086839 #Duplication Level Percentage of deduplicated Percentage of total 1 74.13843451482244 38.61181134273421 2 14.404549536505632 15.00397878159748 3 4.951055459144108 7.73563215572078 4 2.2261999022964623 4.637681450988766 5 1.1952826557019813 3.112557521967914 6 0.7140285791851523 2.2312262437080963 7 0.4704542377895853 1.7151108295795872 8 0.31738024541174203 1.3223506797731277 9 0.23221509957324443 1.0884531224458707 >10 1.2117733516157772 11.396770108048573 >50 0.08734069254179877 3.104270582979216 >100 0.04390442362907362 4.227420288613561 >500 0.0037257999476226133 1.3971407945630459 >1k 0.0033743093865047967 3.198951204747822 >5k 2.108943366565498E-4 0.782356375327682 >10k+ 7.029811221884993E-5 0.4342885172042194 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11862 0.43368514648481776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9311 0.34041834420166395 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6122 0.22382570112797623 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5936 0.21702537763731902 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3956 0.1446348372529033 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3635 0.13289879509967228 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3287 0.12017560921392648 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3213 0.11747010416925639 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3074 0.11238814199075449 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2915 0.10657496223261201 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2902 0.10609967080584565 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2795 0.10218765675476862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.6560878982028136E-5 0.0 5 0.0 0.0 0.0 3.6560878982028136E-5 0.0 6 0.0 0.0 0.0 3.6560878982028136E-5 3.6560878982028136E-5 7 0.0 0.0 0.0 7.312175796405627E-5 3.6560878982028136E-5 8 0.0 0.0 0.0 7.312175796405627E-5 3.6560878982028136E-5 9 0.0 0.0 0.0 1.096826369460844E-4 3.6560878982028136E-5 10-11 0.0 0.0 0.0 1.096826369460844E-4 3.6560878982028136E-5 12-13 0.0 0.0 0.0 1.096826369460844E-4 3.6560878982028136E-5 14-15 0.0 0.0 0.0 1.2796307643709847E-4 3.6560878982028136E-5 16-17 0.0 0.0 0.0 1.4624351592811254E-4 3.6560878982028136E-5 18-19 0.0 0.0 0.0 1.645239554191266E-4 3.6560878982028136E-5 20-21 0.0 0.0 0.0 2.0108483440115475E-4 3.6560878982028136E-5 22-23 0.0 0.0 0.0 2.9248703185622504E-4 3.6560878982028136E-5 24-25 0.0 0.0 0.0 4.752914267663657E-4 3.6560878982028136E-5 26-27 0.0 0.0 0.0 8.957415350596893E-4 3.6560878982028136E-5 28-29 0.0 0.0 0.0 0.0026141028472150118 3.6560878982028136E-5 30-31 0.0 0.0 0.0 0.008335880407902414 3.6560878982028136E-5 32-33 0.0 0.0 0.0 0.01892025487319956 3.6560878982028136E-5 34-35 0.0 0.0 0.0 0.03659743986101016 3.6560878982028136E-5 36-37 0.0 0.0 0.0 0.06502352326953703 3.6560878982028136E-5 38-39 0.0 0.0 0.0 0.10772662992054591 3.6560878982028136E-5 40-41 0.0 0.0 0.0 0.1522943413996382 3.6560878982028136E-5 42-43 0.0 0.0 0.0 0.2013041996750469 3.6560878982028136E-5 44-45 0.0 0.0 0.0 0.266473966460512 3.6560878982028136E-5 46-47 0.0 0.0 0.0 0.3398333701379515 3.6560878982028136E-5 48-49 0.0 0.0 0.0 0.415843437541588 3.6560878982028136E-5 50-51 0.0 0.0 0.0 0.5018163444678272 3.6560878982028136E-5 52-53 0.0 0.0 0.0 0.5816835846040675 3.6560878982028136E-5 54-55 0.0 0.0 0.0 0.6700512291036296 3.6560878982028136E-5 56-57 0.0 0.0 0.0 0.7749078300240864 3.6560878982028136E-5 58-59 0.0 0.0 0.0 0.8809892203904409 3.6560878982028136E-5 60-61 0.0 0.0 0.0 0.9983130810437693 3.6560878982028136E-5 62-63 0.0 0.0 0.0 1.1117980494039845 3.6560878982028136E-5 64-65 0.0 0.0 0.0 1.2328511197134797 3.6560878982028136E-5 66-67 0.0 0.0 0.0 1.3817087384888072 3.6560878982028136E-5 68-69 0.0 0.0 0.0 1.541169012168923 3.6560878982028136E-5 70-71 0.0 0.0 0.0 1.7062231003332888 3.6560878982028136E-5 72-73 0.0 0.0 0.0 1.8705459709180143 3.6560878982028136E-5 74-75 0.0 0.0 0.0 2.0394389513754936 3.6560878982028136E-5 76-77 0.0 0.0 0.0 2.2239982684767714 3.6560878982028136E-5 78-79 0.0 0.0 0.0 2.438921395572624 3.6560878982028136E-5 80-81 0.0 0.0 0.0 2.664118129662426 3.6560878982028136E-5 82-83 0.0 0.0 0.0 2.8911611881408206 3.6560878982028136E-5 84-85 0.0 0.0 0.0 3.1275638316386147 3.6560878982028136E-5 86-87 0.0 5.484131847304221E-5 0.0 3.372082990270419 3.6560878982028136E-5 88-89 0.0 7.312175796405627E-5 0.0 3.6365643888264105 3.6560878982028136E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16385 0.0 25.588903 1 GGTATCA 12275 0.0 22.57688 1 GAGTACT 10590 0.0 22.42246 12-13 TATCAAC 19075 0.0 21.897432 2 GTACATG 8415 0.0 21.823072 1 ATCAACG 19160 0.0 21.725883 3 TACATGG 8090 0.0 21.674381 2 CAACGCA 19215 0.0 21.663696 5 TCAACGC 19270 0.0 21.601864 4 GTAAGGT 1610 0.0 21.54596 4 AGAGTAC 14275 0.0 21.380535 10-11 ACATGGG 7860 0.0 21.341307 3 AACGCAG 19610 0.0 21.251562 6 GTACTTT 11150 0.0 21.11205 14-15 TAAGGTG 1790 0.0 20.972147 5 AGTACTT 10940 0.0 20.879486 12-13 ACGCAGA 21540 0.0 19.29005 7 CGCAGAG 21615 0.0 19.20077 8 AGGTAAG 1600 0.0 19.007668 2 GTGTAAG 1295 0.0 18.784937 1 >>END_MODULE