FastQCFastQC Report
Thu 26 May 2016
SRR937953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735164
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117600.42995593682865085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100080.3659012768521376No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70600.25811980561311865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63170.23095507252947173No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32310.11812819999093291No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31730.11600766900997526No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31280.11436242945578401No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30120.11012136749386874No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29940.10946327167219223No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27640.10105426950632578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG1852.910383E-1028.2417169
TAAGGTG19550.027.4537585
GTAAGGT19650.026.1054574
GTATCAA186800.024.0091291
GGTATCA140950.023.9991681
AGGTAAG20200.022.8140132
GAGTACT115800.022.74380712-13
AGGACCG9000.022.6932185
GGTAAGG21750.021.8410953
GTACTTT121150.021.58261314-15
GGACCGA8050.021.2410856
AGAGTAC155000.021.00612310-11
AGTACTT119600.020.88933812-13
AAGGTAA21250.020.7924351
TATCAAC214900.020.7591922
TCAACGC214700.020.6847214
ATCAACG215800.020.5822963
CAACGCA216250.020.5803895
AACGCAG220300.020.22366
TACATGG80500.019.2399062
Produced by FastQC (version 0.11.5)