##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937953_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2735164 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.031285144144924 31.0 31.0 34.0 30.0 34.0 2 32.30278769390062 34.0 31.0 34.0 31.0 34.0 3 32.36808103645705 34.0 31.0 34.0 30.0 34.0 4 35.96319233508484 37.0 35.0 37.0 35.0 37.0 5 35.89831066802576 37.0 35.0 37.0 35.0 37.0 6 35.95458444173732 37.0 35.0 37.0 35.0 37.0 7 35.91187000121382 37.0 35.0 37.0 35.0 37.0 8 35.8993866546942 37.0 35.0 37.0 35.0 37.0 9 37.51744027049201 39.0 37.0 39.0 35.0 39.0 10-11 37.47323067282254 39.0 37.0 39.0 35.0 39.0 12-13 37.41254418382225 39.0 37.0 39.0 34.0 39.0 14-15 38.542782260954006 40.0 38.0 41.0 34.0 41.0 16-17 38.45588015928844 40.0 38.0 41.0 34.0 41.0 18-19 38.48268677856245 40.0 38.0 41.0 34.0 41.0 20-21 38.37625513497545 40.0 38.0 41.0 34.0 41.0 22-23 38.18414160905891 40.0 38.0 41.0 33.5 41.0 24-25 38.11810333859323 40.0 37.5 41.0 33.0 41.0 26-27 38.04798487403315 40.0 37.0 41.0 33.0 41.0 28-29 37.97800808287913 40.0 37.5 41.0 33.0 41.0 30-31 37.726278753303276 40.0 37.0 41.0 33.0 41.0 32-33 37.4959128593386 39.0 36.5 41.0 32.5 41.0 34-35 37.235003093050366 39.0 36.0 41.0 31.5 41.0 36-37 37.053103945503814 39.0 36.0 40.0 31.0 41.0 38-39 37.02496395097332 39.0 36.0 40.5 31.0 41.0 40-41 36.945829208047485 39.0 36.0 41.0 31.0 41.0 42-43 36.73740313195114 39.0 35.5 40.5 30.5 41.0 44-45 36.475589397930065 39.0 35.0 40.0 30.0 41.0 46-47 36.07979941970573 39.0 35.0 40.0 29.5 41.0 48-49 35.63845677992252 38.0 35.0 40.0 28.0 41.0 50-51 35.25266108357671 38.0 34.5 40.0 27.0 41.0 52-53 34.70955251677779 38.0 34.0 40.0 26.0 41.0 54-55 34.396437105782326 38.0 33.5 40.0 24.5 41.0 56-57 34.075584133163495 37.5 33.0 40.0 24.0 41.0 58-59 33.6534953662742 37.0 33.0 40.0 23.0 41.0 60-61 33.173139160942455 36.5 32.0 39.0 22.0 40.0 62-63 32.72150865542249 36.0 31.0 39.0 20.5 40.0 64-65 32.2420375889709 35.5 31.0 39.0 18.5 40.0 66-67 31.721247062333376 35.0 30.5 38.0 17.5 40.0 68-69 31.167675685991775 35.0 30.0 38.0 13.5 40.0 70-71 30.52256391207255 34.0 29.0 37.0 9.5 39.0 72-73 30.013676145196413 34.0 29.0 36.5 8.0 39.0 74-75 29.48749032964751 34.0 28.0 36.0 7.0 39.0 76-77 28.423305147333032 32.0 27.0 34.5 7.0 36.5 78-79 29.222559780693224 33.5 29.0 35.0 4.0 37.0 80-81 29.020887961380012 34.0 29.0 35.0 2.0 37.0 82-83 28.536820461222803 33.0 27.5 35.0 2.0 36.5 84-85 27.999669672458396 33.0 26.5 35.0 2.0 36.0 86-87 27.40410355649606 32.5 26.0 35.0 2.0 35.5 88-89 26.69208811610565 32.0 25.0 34.0 2.0 35.0 90-91 26.108578498400828 32.0 24.0 34.0 2.0 35.0 92-93 25.43543202528258 31.0 21.5 34.0 2.0 35.0 94-95 24.678178164088152 31.0 19.0 34.0 2.0 35.0 96-97 23.687152031834287 31.0 6.5 34.0 2.0 35.0 98-99 22.544880306994386 30.0 2.0 34.0 2.0 35.0 100-101 19.913842643439295 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1369365058738694 1101 2 0.12591585319170662 1101 3 0.10143289294297375 1101 4 0.07102431624208805 1101 5 0.1205492925401046 1101 6 0.11715785282926561 1101 7 0.16100379823625843 1101 8 0.11930750264889411 1101 9 0.045300019424317384 1101 10-11 0.13721500364218997 1101 12-13 0.24302479605964322 1101 14-15 0.26378481613519256 1101 16-17 0.17975149126376522 1101 18-19 0.23275174223151396 1101 20-21 0.2603559070939383 1101 22-23 0.2647962203990559 1101 24-25 0.2884766739670539 1101 26-27 0.26013270279979395 1101 28-29 0.28280071300698495 1101 30-31 0.3495071250851325 1101 32-33 0.32206634263636147 1101 34-35 0.25618242154293114 1101 36-37 0.2838082413657972 1101 38-39 0.2943699575572083 1101 40-41 0.30959454961318755 1101 42-43 0.3000584590957942 1101 44-45 0.40438562173376624 1101 46-47 0.43522331764842903 1101 48-49 0.4450249706367373 1101 50-51 0.3922145524318523 1101 52-53 0.39718537157184386 1101 54-55 0.4823188671125678 1101 56-57 0.491503701347213 1101 58-59 0.3240567672553212 1101 60-61 0.5100591398158585 1101 62-63 0.43922039984713734 1101 64-65 0.5739481952919832 1101 66-67 0.562631896952368 1101 68-69 0.4906182259012084 1101 70-71 0.48195593962444505 1101 72-73 0.38476846070033943 1101 74-75 0.7622788112064249 1101 76-77 0.5249860597289349 1101 78-79 0.5693712176066263 1101 80-81 0.6972970148328841 1101 82-83 0.7841249803220585 1101 84-85 0.8501474885333202 1101 86-87 0.9192976697406223 1101 88-89 1.0084717384842286 1101 90-91 1.0557687318642905 1101 92-93 1.321972948996244 1101 94-95 1.1092598385820693 1101 96-97 1.4395380942380882 1101 98-99 1.6673908443886383 1101 100-101 1.728858635299762 1102 1 0.2671023630621292 1102 2 0.2568912128084051 1102 3 0.24311916895072727 1102 4 0.16480263567411413 1102 5 0.18990920761153518 1102 6 0.15041793791638725 1102 7 0.20403124506761827 1102 8 0.1702744443628461 1102 9 0.21325346726899852 1102 10-11 0.20815853666768191 1102 12-13 0.310808948925299 1102 14-15 0.344914371838577 1102 16-17 0.38404844143165207 1102 18-19 0.40091722320870815 1102 20-21 0.432578053241123 1102 22-23 0.42728819348194946 1102 24-25 0.3696158871958488 1102 26-27 0.26052167044636576 1102 28-29 0.300776538923607 1102 30-31 0.31533152100331563 1102 32-33 0.3681950082618428 1102 34-35 0.45361798819102717 1102 36-37 0.4653557372919792 1102 38-39 0.3343992472462105 1102 40-41 0.48785351399493493 1102 42-43 0.41893459700813906 1102 44-45 0.41451478032546873 1102 46-47 0.30843355837306063 1102 48-49 0.4238612244105653 1102 50-51 0.33119210877522676 1102 52-53 0.3526426301751471 1102 54-55 0.31929443285257264 1102 56-57 0.41273786102358656 1102 58-59 0.43931846817617526 1102 60-61 0.4704025297081742 1102 62-63 0.5020031076875355 1102 64-65 0.5821162996566756 1102 66-67 0.4406773867799032 1102 68-69 0.761559885078487 1102 70-71 0.7721677493365995 1102 72-73 0.6323673764629341 1102 74-75 0.688540217356536 1102 76-77 0.7741476928308941 1102 78-79 0.736774835142672 1102 80-81 0.9429521013578253 1102 82-83 1.126216295828371 1102 84-85 0.8737020488672691 1102 86-87 0.9144003674334122 1102 88-89 0.6831902108078474 1102 90-91 0.7857749515989418 1102 92-93 0.6974471587187772 1102 94-95 0.8658028480832023 1102 96-97 0.7288144933360421 1102 98-99 0.7255328229849631 1102 100-101 0.9238057315197032 1103 1 0.1227366601051969 1103 2 0.17951440806530883 1103 3 0.17043908684485132 1103 4 0.0945329732675404 1103 5 0.13321950159749463 1103 6 0.1181649340894495 1103 7 0.11072931577390932 1103 8 0.13475317131916142 1103 9 0.14129739960453236 1103 10-11 0.10654057611154144 1103 12-13 0.13538823267163025 1103 14-15 0.23838664930478615 1103 16-17 0.26208144066320926 1103 18-19 0.2768372525857856 1103 20-21 0.31920572169943995 1103 22-23 0.3020659738049929 1103 24-25 0.3748800205755458 1103 26-27 0.36204404443626714 1103 28-29 0.29085525033061543 1103 30-31 0.3287570256094483 1103 32-33 0.3431668077952281 1103 34-35 0.33517491773116603 1103 36-37 0.2868861774830407 1103 38-39 0.30498128405225344 1103 40-41 0.384239342193176 1103 42-43 0.4394385055060397 1103 44-45 0.45841553134294344 1103 46-47 0.37569706839229156 1103 48-49 0.5151544474826721 1103 50-51 0.48502976710565804 1103 52-53 0.483633086095999 1103 54-55 0.5337060687295292 1103 56-57 0.5934851161203198 1103 58-59 0.5837052043426709 1103 60-61 0.7626238518293533 1103 62-63 0.7994632261537831 1103 64-65 0.7769141552411298 1103 66-67 0.6013492416382604 1103 68-69 0.6235427405831153 1103 70-71 0.6914179258281372 1103 72-73 0.6249932581396749 1103 74-75 0.6522773674885336 1103 76-77 0.6509585445786961 1103 78-79 0.4946225477637789 1103 80-81 0.529305296843809 1103 82-83 0.713511681919659 1103 84-85 0.8716268416068509 1103 86-87 0.9660723687606563 1103 88-89 0.7689526021796489 1103 90-91 0.7867530305519175 1103 92-93 1.0241955702949532 1103 94-95 0.8887583402845998 1103 96-97 0.9880505366214152 1103 98-99 0.9882599625587041 1103 100-101 1.0843734615782452 1104 1 0.21104994289361656 1104 2 0.13411806101150603 1104 3 0.13974182216968956 1104 4 0.05840412360277725 1104 5 0.11655124045409337 1104 6 0.13576995031325367 1104 7 0.16072692405881384 1104 8 0.05861411700330876 1104 9 0.1360300457183996 1104 10-11 0.19630184308275744 1104 12-13 0.21813798752083358 1104 14-15 0.2624857699508638 1104 16-17 0.20979316724233854 1104 18-19 0.329218802895511 1104 20-21 0.2063823418871067 1104 22-23 0.24985184355219303 1104 24-25 0.23129001635018653 1104 26-27 0.29800686921124964 1104 28-29 0.36158962645173887 1104 30-31 0.3361274943058845 1104 32-33 0.3146192963747865 1104 34-35 0.37817357039833155 1104 36-37 0.383131782706549 1104 38-39 0.3108224502525303 1104 40-41 0.440832702792072 1104 42-43 0.5474373973447086 1104 44-45 0.4850133963385943 1104 46-47 0.5605214367801992 1104 48-49 0.5963346392982274 1104 50-51 0.7436411373192371 1104 52-53 0.7031729998348055 1104 54-55 0.671355849887604 1104 56-57 0.6999022870402598 1104 58-59 0.8951712858363763 1104 60-61 0.8788000175911534 1104 62-63 0.9052748945345215 1104 64-65 0.9148780483155221 1104 66-67 0.8237428701092426 1104 68-69 1.0347568947530696 1104 70-71 0.9702630064687945 1104 72-73 1.0020232263298645 1104 74-75 0.8799387484648342 1104 76-77 1.0909326741937129 1104 78-79 1.2832925947211216 1104 80-81 1.257627787735359 1104 82-83 1.0873851841080757 1104 84-85 1.22805218422004 1104 86-87 1.1628959717997276 1104 88-89 1.1870423393301586 1104 90-91 1.3668914311323057 1104 92-93 1.423969353908518 1104 94-95 1.266006353167974 1104 96-97 1.5478125211071578 1104 98-99 1.872941226659858 1104 100-101 1.8874137300881237 1105 1 0.1523389632965575 1105 2 0.12782547876955874 1105 3 0.07396652854629338 1105 4 0.0684686020216887 1105 5 0.04496740937659638 1105 6 0.013909391743837318 1105 7 -0.012401283879519553 1105 8 0.03853082476812375 1105 9 0.06531384209080215 1105 10-11 0.07240007677592786 1105 12-13 0.04869491301445805 1105 14-15 0.17855651355325364 1105 16-17 0.08476348525063315 1105 18-19 0.04362552837818612 1105 20-21 0.13088505436450504 1105 22-23 0.04295694520160254 1105 24-25 0.0801995456394593 1105 26-27 0.08708891936925056 1105 28-29 0.010953975183170428 1105 30-31 0.15292110856785968 1105 32-33 0.16540107437175777 1105 34-35 0.23535752568354695 1105 36-37 0.2641252618012899 1105 38-39 0.11762921592005426 1105 40-41 0.04755087913049749 1105 42-43 0.2233054814406472 1105 44-45 0.2737027309140032 1105 46-47 0.23663839296044387 1105 48-49 0.3910585511107385 1105 50-51 0.43018560550927987 1105 52-53 0.2893603708323056 1105 54-55 0.40446451337601275 1105 56-57 0.3088785779785752 1105 58-59 0.4816623264413451 1105 60-61 0.2966425477754555 1105 62-63 0.34171890059877086 1105 64-65 0.3224346267581737 1105 66-67 0.3973450766228339 1105 68-69 0.5066777305979571 1105 70-71 0.6996294557719551 1105 72-73 0.3900011063963511 1105 74-75 0.316766337191158 1105 76-77 0.29544844674746074 1105 78-79 0.5042978552833439 1105 80-81 0.6027494382367564 1105 82-83 0.9010667557010414 1105 84-85 0.8579040482491216 1105 86-87 0.6393010716225511 1105 88-89 0.5550419557475017 1105 90-91 0.5902484187298 1105 92-93 0.7681891285080376 1105 94-95 0.667580868666537 1105 96-97 1.1950123676740922 1105 98-99 1.2921308626216508 1105 100-101 1.1096656343020577 1106 1 -0.1380231354788286 1106 2 -0.06932216472171149 1106 3 -0.018714901573659404 1106 4 -0.037148766536098776 1106 5 -0.06115162992393408 1106 6 -0.044318008437123524 1106 7 0.007141479072096502 1106 8 -0.14585780880619126 1106 9 -0.14646735779811593 1106 10-11 -0.02814347451543142 1106 12-13 -0.11304463028599088 1106 14-15 -0.298783198165232 1106 16-17 -0.19959760122485903 1106 18-19 -0.12470740182686768 1106 20-21 0.01387147342082784 1106 22-23 0.04888517045176144 1106 24-25 0.006316210962367563 1106 26-27 -0.1079602174883263 1106 28-29 -0.004265237014600132 1106 30-31 0.21076329728563792 1106 32-33 0.050984311402217486 1106 34-35 -0.012440946012361564 1106 36-37 -0.18050618937091656 1106 38-39 -0.13418150191702694 1106 40-41 0.021646528032640333 1106 42-43 -0.16854752007332507 1106 44-45 -0.017056897132967208 1106 46-47 -0.09206792361171523 1106 48-49 -0.021978841276400374 1106 50-51 0.03792611631759257 1106 52-53 -0.15534162778810412 1106 54-55 -0.6018321263258528 1106 56-57 -0.24991320178796883 1106 58-59 -0.21722772704034554 1106 60-61 -0.2805654753730664 1106 62-63 -0.22841758071846385 1106 64-65 -0.2959966219540533 1106 66-67 -0.2940938225682217 1106 68-69 -0.5642851636838948 1106 70-71 -0.4852877237634594 1106 72-73 -0.3411537665286595 1106 74-75 -0.41862698162956136 1106 76-77 -0.27190980887170113 1106 78-79 -0.22846281193874773 1106 80-81 -0.42752101604401815 1106 82-83 -0.47756251857847687 1106 84-85 -0.17676946263982174 1106 86-87 0.09183159739140478 1106 88-89 -0.10672334297055386 1106 90-91 -0.16812433159046236 1106 92-93 -0.17565174332404254 1106 94-95 -0.3647231438035625 1106 96-97 0.019849772603009797 1106 98-99 0.12550898798558308 1106 100-101 0.15959643061477635 1107 1 0.07383473106665406 1107 2 0.12190662476226066 1107 3 0.01725355569087128 1107 4 0.04941264617780661 1107 5 0.007779769596197639 1107 6 0.05327796184557343 1107 7 0.04351277426532363 1107 8 0.06253316666848008 1107 9 0.047125177610290336 1107 10-11 0.12112948187055395 1107 12-13 0.11884199879202129 1107 14-15 0.205575025582327 1107 16-17 0.1501345330563595 1107 18-19 0.15986916198949075 1107 20-21 0.1639386369687159 1107 22-23 0.14377333394565994 1107 24-25 0.20737952628506662 1107 26-27 0.2156177956844303 1107 28-29 0.14568126629962563 1107 30-31 0.24031478049857213 1107 32-33 0.17546051457589584 1107 34-35 0.288303372324215 1107 36-37 0.32839041221568266 1107 38-39 0.28900022004069825 1107 40-41 0.29909398348751637 1107 42-43 0.30955420056550054 1107 44-45 0.3282085386559501 1107 46-47 0.39773521145176716 1107 48-49 0.40643391994105826 1107 50-51 0.3694906423044628 1107 52-53 0.2760976743800043 1107 54-55 0.3874476300408247 1107 56-57 0.29850978090433955 1107 58-59 0.40916004484539314 1107 60-61 0.3255908008944246 1107 62-63 0.43963082482225246 1107 64-65 0.44562525706745504 1107 66-67 0.39402049929976357 1107 68-69 0.577854383579794 1107 70-71 0.5841662181944294 1107 72-73 0.5424085106381717 1107 74-75 0.5200475143332177 1107 76-77 0.40426799031430605 1107 78-79 0.6570143789763243 1107 80-81 0.5892118106596911 1107 82-83 0.66392868899392 1107 84-85 0.9186832951752599 1107 86-87 0.8230086009366673 1107 88-89 0.9016649325820616 1107 90-91 0.7898891326580291 1107 92-93 0.9787587107741764 1107 94-95 0.8569115467065238 1107 96-97 0.8934166037976681 1107 98-99 1.0556551947384456 1107 100-101 1.3092056826025633 1108 1 0.024448929561636135 1108 2 0.09001431005277993 1108 3 0.06518529781984483 1108 4 0.07889854669795682 1108 5 -0.04634326734223748 1108 6 0.012119812798147223 1108 7 0.08088453083371405 1108 8 0.07385547800767966 1108 9 0.07756413072475254 1108 10-11 0.04748386904087454 1108 12-13 0.10049919618241887 1108 14-15 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10.0 4 37.0 5 71.0 6 150.0 7 356.0 8 973.0 9 1724.0 10 2881.0 11 4632.0 12 6812.0 13 9975.0 14 13587.0 15 18407.0 16 22955.0 17 30931.0 18 30421.0 19 24267.0 20 22119.0 21 22200.0 22 23718.0 23 25813.0 24 29930.0 25 34601.0 26 41421.0 27 50451.0 28 60766.0 29 73446.0 30 88851.0 31 108859.0 32 136808.0 33 176740.0 34 237964.0 35 329383.0 36 439693.0 37 457412.0 38 198711.0 39 8085.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42688921029964 19.724923258715016 15.579285190942846 22.268902340042498 2 16.10507324543345 20.93837232857658 39.34330318585275 23.613251240137227 3 20.133213260777918 24.07048887884628 30.317040540575128 25.479257319800674 4 13.477180892992157 17.666253284994976 37.092291358031915 31.76427446398095 5 13.613370167200213 37.534348945803615 34.41314670710787 14.439134179888299 6 31.8793548608821 37.02208498070681 16.876148469450804 14.222411688960287 7 28.346197887951142 33.0460257593329 20.291653443815434 18.316122908900525 8 26.26175803026669 35.00664679696046 19.260574915032144 19.471020257740705 9 25.689192361099433 17.280939528904728 20.289431177284822 36.74043693271102 10-11 24.900956578837686 26.733534076932862 27.185426541150733 21.18008280307872 12-13 26.286248283466733 23.94828975520298 27.483288022217316 22.282173939112976 14-15 22.54857478381552 25.574261726170715 26.286156881269278 25.591006608744483 16-17 21.812627540690997 28.716053359286754 27.150493947969707 22.320825152052542 18-19 21.79050323856266 27.139670601104726 29.587110681480162 21.48271547885246 20-21 22.285446137781868 26.013741771974185 30.075564024680055 21.62524806556389 22-23 22.06304631093419 25.987289976030688 30.035237375162875 21.914426337872246 24-25 22.207064867162398 26.208673634946823 29.911709727103624 21.672551770787155 26-27 21.680192423199394 26.41434434135188 30.045226034178707 21.86023720127002 28-29 21.644281959743907 27.130974071568296 29.686945588444715 21.537798380243082 30-31 21.90369571989102 26.44102876463715 29.49865163478314 22.156623880688688 32-33 21.647051339260006 26.45907525679136 29.61877572643679 22.275097677511845 34-35 22.52621436263674 26.51836696633774 29.352973764611022 21.602444906414497 36-37 22.483031426047585 25.972612626578762 29.613177938587 21.93117800878666 38-39 21.859733861945433 26.64613065707003 29.40838118578269 22.08575429520185 40-41 22.47344631652773 26.452650212469692 29.27591367817744 21.79798979282514 42-43 22.069687843129227 26.472418580450974 29.036570833938164 22.421322742481642 44-45 22.207680669700345 26.529775794000454 29.237892649339653 22.02465088695955 46-47 22.75454881482383 27.017896191195916 28.452911215894627 21.77464377808562 48-49 22.41960628320642 27.02057353782077 28.383160936601975 22.176659242370842 50-51 22.308771978572402 27.268200371166046 28.552712012881127 21.87031563738043 52-53 22.85755077209264 27.100842947625807 28.103780979860804 21.937825300420744 54-55 22.299686600145368 27.168809621653402 28.354881096709374 22.17662268149186 56-57 22.543931552184805 27.140822268792657 28.147398108486364 22.167848070536174 58-59 22.678914317386454 26.914163099543575 28.026052551145014 22.38087003192496 60-61 22.175379611606473 27.24377770400605 28.482679649191056 22.09816303519643 62-63 22.174995722377158 27.222243346285634 28.561980195703075 22.040780735634133 64-65 21.84583812890196 27.456817945834327 28.26768340033724 22.429660524926476 66-67 21.931719633630745 27.18546310202971 28.20629768452641 22.67651957981313 68-69 22.40993245173812 26.94400738824538 28.289707842936167 22.356352317080326 70-71 22.441378286640216 27.048487768923547 27.88918690067579 22.62094704376045 72-73 22.32447487609518 27.019111102661487 28.137362147205796 22.519051874037537 74-75 22.212123294983407 27.72038532241577 27.713804364199003 22.35368701840182 76-77 22.293562652915877 27.11148216340958 28.02071466281364 22.574240520860904 78-79 22.438782464232492 27.367901888150037 27.944192743104253 22.24912290451322 80-81 22.687103954278427 27.274232916198077 27.65984050682153 22.378822622701968 82-83 22.562914897576487 27.358826512350454 27.886089421361724 22.192169168711334 84-85 22.436371686641376 27.598828335588294 27.79913300635977 22.165666971410563 86-87 22.614353229406237 27.57557205635002 27.395874712013523 22.414200002230224 88-89 22.44543467626415 27.744773435723367 27.125870423762834 22.68392146424965 90-91 22.424837038605098 27.50622724764977 27.3625243450376 22.70641136870754 92-93 22.65932497829649 27.265569630110747 27.629918068673586 22.445187322919182 94-95 22.401362404594387 27.142668593181252 27.680022112019607 22.775946890204757 96-97 22.403889200773556 26.907952669008633 27.88568215391877 22.802475976299043 98-99 22.3771225418293 27.22308424650222 27.22957380252153 23.17021940914695 100-101 22.451414247920784 26.808904328954313 27.544381250996285 23.19530017212862 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 711.0 1 477.0 2 352.5 3 459.5 4 386.0 5 379.5 6 723.0 7 1767.0 8 2977.0 9 3502.0 10 3046.0 11 2409.0 12 2429.0 13 2715.0 14 2986.5 15 3180.5 16 3392.5 17 3731.0 18 4076.0 19 4495.5 20 5135.0 21 6104.5 22 7461.0 23 9161.0 24 11442.0 25 14607.0 26 18570.0 27 23250.0 28 27195.5 29 30670.0 30 34967.5 31 41414.0 32 49901.5 33 53623.5 34 53707.5 35 56147.5 36 60494.5 37 65176.5 38 69522.0 39 73176.5 40 77300.0 41 82438.0 42 85902.5 43 89010.5 44 92272.0 45 96348.5 46 98623.5 47 104949.0 48 113533.0 49 109522.5 50 99955.0 51 94230.5 52 91305.0 53 90724.5 54 91518.0 55 85738.0 56 77011.5 57 70221.0 58 64709.0 59 57586.5 60 46270.5 61 38006.0 62 32388.5 63 24583.5 64 16885.5 65 12235.5 66 9206.0 67 6981.0 68 5323.0 69 4106.0 70 3092.5 71 2124.0 72 1509.0 73 1211.0 74 909.5 75 646.0 76 458.5 77 302.5 78 196.5 79 120.0 80 69.0 81 35.0 82 13.5 83 8.5 84 4.5 85 3.5 86 2.5 87 2.0 88 2.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01411249928706286 3 0.005520692726286248 4 0.0 5 0.0 6 7.312175796405626E-5 7 0.0 8 4.387305477843376E-4 9 2.193652738921688E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.74206592365211E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0055389731657772626 26-27 0.020711737943318938 28-29 1.8280439491014065E-5 30-31 0.0 32-33 5.666936242214361E-4 34-35 0.018243878612032038 36-37 0.003948574930059038 38-39 0.016653480376313815 40-41 0.015392130051433844 42-43 0.00822619777095633 44-45 0.003290479108382532 46-47 3.656087898202813E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.1076747134723917E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.656087898202813E-5 84-85 0.002943150758053265 86-87 3.4732835032926727E-4 88-89 7.312175796405626E-5 90-91 2.5592615287419693E-4 92-93 2.74206592365211E-4 94-95 0.0 96-97 1.8280439491014065E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2735164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.059890451526535 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29910536669385 38.68003286036509 2 14.247173557925397 14.834125893389633 3 4.935739035670203 7.7086210048294195 4 2.231677408726087 4.647235256857067 5 1.1998802518891118 3.1232817234148613 6 0.6947178746682824 2.1700161869968877 7 0.4793865035989513 1.7469766202911212 8 0.3246935581935673 1.3522808855898774 9 0.23656176720232236 1.1083841717015184 >10 1.2140176738836317 11.52165263370349 >50 0.08684490633149199 3.101318490645315 >100 0.043105268517531894 4.325681326941852 >500 0.003372749322520355 1.2643580346033345 >1k 0.0034430149333758158 3.130423779845024 >5k 1.4053122177044145E-4 0.4893333360948256 >10k+ 1.4053122177044145E-4 0.7962777947306694 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11760 0.42995593682865085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10008 0.3659012768521376 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7060 0.25811980561311865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6317 0.23095507252947173 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3231 0.11812819999093291 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3173 0.11600766900997526 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3128 0.11436242945578401 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3012 0.11012136749386874 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2994 0.10946327167219223 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2764 0.10105426950632578 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.312175796405627E-5 0.0 10-11 0.0 0.0 0.0 7.312175796405627E-5 0.0 12-13 0.0 0.0 0.0 7.312175796405627E-5 0.0 14-15 0.0 0.0 0.0 7.312175796405627E-5 0.0 16-17 0.0 0.0 0.0 1.096826369460844E-4 0.0 18-19 0.0 0.0 0.0 1.2796307643709847E-4 0.0 20-21 0.0 0.0 0.0 1.645239554191266E-4 0.0 22-23 0.0 0.0 0.0 2.9248703185622504E-4 0.0 24-25 0.0 0.0 0.0 4.752914267663657E-4 0.0 26-27 0.0 0.0 0.0 9.323024140417174E-4 0.0 28-29 0.0 0.0 0.0 0.0026506637261970397 0.0 30-31 0.0 0.0 0.0 0.008281039089429372 0.0 32-33 0.0 0.0 0.0 0.018518085204397253 0.0 34-35 0.0 0.0 0.0 0.03582966140238757 0.0 36-37 0.0 0.0 0.0 0.06409122085549532 0.0 38-39 0.0 0.0 0.0 0.10650184047464795 0.0 40-41 0.0 0.0 0.0 0.1510329910747582 0.0 42-43 0.0 0.0 0.0 0.19942131440747246 0.0 44-45 0.0 0.0 0.0 0.2640426680082072 0.0 46-47 0.0 0.0 0.0 0.33656117146906 0.0 48-49 0.0 0.0 0.0 0.4116389364586548 0.0 50-51 0.0 0.0 0.0 0.49646017569696 0.0 52-53 0.0 0.0 0.0 0.5755047960561048 3.6560878982028136E-5 54-55 0.0 0.0 0.0 0.662757333746715 3.6560878982028136E-5 56-57 0.0 0.0 0.0 0.7659138537945074 3.6560878982028136E-5 58-59 0.0 0.0 0.0 0.8704596872436168 3.6560878982028136E-5 60-61 0.0 0.0 0.0 0.9852791276866761 3.6560878982028136E-5 62-63 0.0 0.0 0.0 1.0962779562761136 1.096826369460844E-4 64-65 0.0 0.0 0.0 1.2141502301141722 1.096826369460844E-4 66-67 0.0 0.0 0.0 1.3600281372524645 1.096826369460844E-4 68-69 0.0 0.0 0.0 1.5152473489706648 1.096826369460844E-4 70-71 0.0 0.0 0.0 1.6757130468227865 1.096826369460844E-4 72-73 0.0 3.6560878982028136E-5 0.0 1.836233585993381 1.096826369460844E-4 74-75 0.0 3.6560878982028136E-5 0.0 2.0019640504189145 1.096826369460844E-4 76-77 0.0 3.6560878982028136E-5 0.0 2.1832694492907923 1.096826369460844E-4 78-79 0.0 3.6560878982028136E-5 0.0 2.393878392666765 1.096826369460844E-4 80-81 0.0 3.6560878982028136E-5 0.0 2.6134264709538444 1.096826369460844E-4 82-83 0.0 3.6560878982028136E-5 0.0 2.835223043298318 1.096826369460844E-4 84-85 0.0 3.6560878982028136E-5 0.0 3.0662329571462625 1.096826369460844E-4 86-87 0.0 3.6560878982028136E-5 0.0 3.30413459668232 1.096826369460844E-4 88-89 0.0 3.6560878982028136E-5 0.0 3.5606822844992108 1.096826369460844E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGCG 185 2.910383E-10 28.241716 9 TAAGGTG 1955 0.0 27.453758 5 GTAAGGT 1965 0.0 26.105457 4 GTATCAA 18680 0.0 24.009129 1 GGTATCA 14095 0.0 23.999168 1 AGGTAAG 2020 0.0 22.814013 2 GAGTACT 11580 0.0 22.743807 12-13 AGGACCG 900 0.0 22.693218 5 GGTAAGG 2175 0.0 21.841095 3 GTACTTT 12115 0.0 21.582613 14-15 GGACCGA 805 0.0 21.241085 6 AGAGTAC 15500 0.0 21.006123 10-11 AGTACTT 11960 0.0 20.889338 12-13 AAGGTAA 2125 0.0 20.792435 1 TATCAAC 21490 0.0 20.759192 2 TCAACGC 21470 0.0 20.684721 4 ATCAACG 21580 0.0 20.582296 3 CAACGCA 21625 0.0 20.580389 5 AACGCAG 22030 0.0 20.2236 6 TACATGG 8050 0.0 19.239906 2 >>END_MODULE