FastQCFastQC Report
Thu 26 May 2016
SRR937952_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937952_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807091
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115980.4131679379115248No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91660.326530205112695No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60110.21413627132144986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59300.21125072183267304No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40080.14278126359280835No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37260.1327352764837335No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34320.12226180056150654No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32940.11734567921025717No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31020.11050585819982323No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA30740.10950838430246827No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29070.10355916498610127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA166300.024.9392621
GGTATCA124800.024.1898041
TAAGGTG16750.023.8494835
GTACATG87200.023.7536541
TACATGG85650.023.3759462
GAGTACT106950.022.4872412-13
ACATGGG86400.021.9070473
AGAGTAC143650.021.58943610-11
GTAAGGT15950.021.467744
GTACTTT111350.021.36388614-15
TATCAAC194350.021.1908252
ATCAACG193700.021.1637273
TCAACGC194850.020.9900054
CAACGCA196150.020.8024025
AACGCAG200600.020.3365756
AGTACTT111650.020.30607812-13
GTAGGAC6950.019.8439313
GGTAAGG17750.019.0228023
TATAGGG6250.019.0228022
AGGTAAG16450.018.7915232
Produced by FastQC (version 0.11.5)