##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937952_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2807091 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48790723207762 33.0 31.0 34.0 31.0 34.0 2 32.63593983949932 34.0 31.0 34.0 31.0 34.0 3 32.67233231840364 34.0 31.0 34.0 31.0 34.0 4 36.15138198227275 37.0 37.0 37.0 35.0 37.0 5 36.15969129607839 37.0 37.0 37.0 35.0 37.0 6 36.208988593529746 37.0 37.0 37.0 35.0 37.0 7 36.16935610566241 37.0 37.0 37.0 35.0 37.0 8 36.1675314409116 37.0 37.0 37.0 35.0 37.0 9 37.902022770191635 39.0 38.0 39.0 35.0 39.0 10-11 37.82905684211876 39.0 38.0 39.0 35.0 39.0 12-13 37.715837320557114 39.0 38.0 39.0 35.0 39.0 14-15 39.01711255531082 40.0 38.5 41.0 36.0 41.0 16-17 38.856364827502915 40.0 38.0 41.0 36.0 41.0 18-19 39.03698633211392 40.0 39.0 41.0 36.0 41.0 20-21 39.03953256235726 40.0 39.0 41.0 36.0 41.0 22-23 38.94735457453998 40.0 39.0 41.0 36.0 41.0 24-25 38.84336453645429 40.0 39.0 41.0 35.5 41.0 26-27 38.679413492473174 40.0 38.0 41.0 35.0 41.0 28-29 38.52320765518468 40.0 38.0 41.0 35.0 41.0 30-31 38.31604016399896 40.0 38.0 41.0 34.5 41.0 32-33 38.04057189453424 40.0 38.0 41.0 34.0 41.0 34-35 37.81564776489255 40.0 38.0 41.0 33.5 41.0 36-37 37.56847640493308 40.0 37.0 41.0 33.0 41.0 38-39 37.282717589134094 40.0 37.0 41.0 32.5 41.0 40-41 37.19081693468434 40.0 37.0 41.0 32.0 41.0 42-43 36.983271294019325 40.0 36.5 41.0 31.5 41.0 44-45 36.682931191044396 39.0 36.0 41.0 31.0 41.0 46-47 36.31385266811799 39.0 36.0 41.0 30.0 41.0 48-49 35.913133382565796 39.0 35.0 40.0 29.0 41.0 50-51 35.189293293306136 38.5 34.5 39.5 27.5 40.5 52-53 35.2636644127319 38.0 35.0 40.0 28.0 40.5 54-55 35.3542633637456 39.0 35.0 40.0 27.5 41.0 56-57 35.05131522276976 38.0 35.0 40.0 26.5 41.0 58-59 34.65599886145479 38.0 34.0 40.0 26.0 41.0 60-61 34.15850038349309 37.5 34.0 40.0 24.5 41.0 62-63 33.64447946290306 37.0 33.0 40.0 22.5 41.0 64-65 33.11996066390438 36.0 32.5 39.0 21.0 41.0 66-67 32.55108117264456 36.0 32.0 39.0 18.5 40.0 68-69 31.87459722538386 35.0 31.0 38.5 13.5 40.0 70-71 31.28355226104177 35.0 30.5 38.0 8.0 40.0 72-73 30.59410845604934 35.0 30.0 37.0 4.5 39.0 74-75 29.93255989919814 34.0 29.0 36.5 2.0 39.0 76-77 29.198714078025972 34.0 28.5 36.0 2.0 38.5 78-79 28.435466288766555 34.0 26.5 35.0 2.0 37.5 80-81 27.71184902805075 33.0 26.0 35.0 2.0 37.0 82-83 26.973633914967486 32.5 25.0 35.0 2.0 36.0 84-85 26.240638796533492 32.0 23.5 35.0 2.0 36.0 86-87 25.50580262627752 31.5 20.5 34.5 2.0 35.5 88-89 24.60940008713647 31.0 17.0 34.0 2.0 35.0 90-91 23.689219195245183 30.5 7.5 34.0 2.0 35.0 92-93 22.733112321616936 30.0 2.0 34.0 2.0 35.0 94-95 21.61503207412941 29.0 2.0 34.0 2.0 35.0 96-97 20.30128431889098 28.0 2.0 34.0 2.0 35.0 98-99 18.662053706132077 26.0 2.0 33.5 2.0 35.0 100-101 14.4082028334671 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14250497010161212 1101 2 0.11382272457937148 1101 3 0.0980042216918946 1101 4 -0.03436748856246652 1101 5 0.0761411390836173 1101 6 0.05168558258696265 1101 7 0.03128813596369895 1101 8 0.06169170612129449 1101 9 0.05869441013810217 1101 10-11 0.12723914346687337 1101 12-13 0.14849044025942248 1101 14-15 0.21192734239735955 1101 16-17 0.20973949028945782 1101 18-19 0.23754071129776833 1101 20-21 0.19098274384879232 1101 22-23 0.14916310921433507 1101 24-25 0.15772811219783733 1101 26-27 0.13722244126702066 1101 28-29 0.12334381501635505 1101 30-31 0.10897125627045057 1101 32-33 0.024967578346327457 1101 34-35 -0.09386637076473647 1101 36-37 0.024557423114735855 1101 38-39 -0.0012505003519933666 1101 40-41 0.04145202911227841 1101 42-43 0.04845230610777662 1101 44-45 0.20826381586334008 1101 46-47 0.18444464096735658 1101 48-49 0.23321660895518193 1101 50-51 0.2257624342759712 1101 52-53 0.21080156791794735 1101 54-55 0.18776055252947543 1101 56-57 0.07011404965601287 1101 58-59 0.14644101380105923 1101 60-61 0.03703348891414748 1101 62-63 0.038305255424596396 1101 64-65 0.021451818526379896 1101 66-67 0.029619180636849762 1101 68-69 -0.025520785679752578 1101 70-71 -0.07205381387079157 1101 72-73 -0.03560817617498202 1101 74-75 -0.25830794549797886 1101 76-77 -0.4877079353533347 1101 78-79 -0.4861866082228268 1101 80-81 -0.5486060525117438 1101 82-83 -0.6129682147956323 1101 84-85 -0.663556049995325 1101 86-87 -0.7265453805825643 1101 88-89 -0.5501191385128301 1101 90-91 -0.6357211947475072 1101 92-93 -0.9199325114693799 1101 94-95 -0.9433857846641729 1101 96-97 -0.8506762225111473 1101 98-99 -0.6891204424067787 1101 100-101 -1.0678759074931996 1102 1 0.0363154155795371 1102 2 0.06059367920086345 1102 3 0.08952029244360915 1102 4 0.043959410114972286 1102 5 0.03135346570538644 1102 6 0.022096804763030775 1102 7 0.019199607808957353 1102 8 0.04822886257081649 1102 9 0.1019120047797486 1102 10-11 0.13418831649443064 1102 12-13 0.16173418491139557 1102 14-15 0.15270715857274553 1102 16-17 0.11995362701284762 1102 18-19 0.09628795642525034 1102 20-21 0.10067853329141485 1102 22-23 0.15145276008602337 1102 24-25 0.0935054657271408 1102 26-27 0.14197480915230187 1102 28-29 -4.5096371999875373E-4 1102 30-31 -0.019436373506458438 1102 32-33 -0.07993972629321178 1102 34-35 -0.05510771178334295 1102 36-37 0.04667300633061444 1102 38-39 -0.0901398242705298 1102 40-41 0.08795894438135576 1102 42-43 0.10595722005134434 1102 44-45 0.2202226862691461 1102 46-47 0.16331404525666926 1102 48-49 0.14382993266490018 1102 50-51 0.08576731701088391 1102 52-53 0.16171837871183214 1102 54-55 0.11266779191456067 1102 56-57 -0.016037140925561744 1102 58-59 0.06276793177111273 1102 60-61 0.1796666590546252 1102 62-63 -0.005312802920819593 1102 64-65 -0.009584615017359965 1102 66-67 0.03409939492110681 1102 68-69 -0.2959979857759869 1102 70-71 -0.36932101437557563 1102 72-73 -0.4837726678825547 1102 74-75 -0.4079971073585149 1102 76-77 -0.8430793977111648 1102 78-79 -0.8601425370716491 1102 80-81 -0.8410793918144392 1102 82-83 -0.7943362118586172 1102 84-85 -0.6418902968909173 1102 86-87 -0.6625844611871265 1102 88-89 -0.6068505744738175 1102 90-91 -0.6770224365112583 1102 92-93 -0.8288016263694082 1102 94-95 -0.9192592296223303 1102 96-97 -1.2662044827935475 1102 98-99 -1.4759332421516653 1102 100-101 -1.3749380132712261 1103 1 0.09862522115857075 1103 2 0.15420807762295397 1103 3 0.13525852856646026 1103 4 0.03175879022204242 1103 5 0.08518905319481718 1103 6 0.10370782195097661 1103 7 0.12680738464749908 1103 8 0.07829366955869688 1103 9 0.10571237108011644 1103 10-11 0.1329309200447213 1103 12-13 0.06674792117513562 1103 14-15 0.09389305600863906 1103 16-17 0.09357295640140961 1103 18-19 0.11090827200710152 1103 20-21 0.1797994124122937 1103 22-23 0.13077263892513713 1103 24-25 0.16661584893368087 1103 26-27 0.13249678717428282 1103 28-29 0.19895027606585813 1103 30-31 0.16916781077080856 1103 32-33 0.16251589500856056 1103 34-35 0.05712989035810523 1103 36-37 -0.021303888569363494 1103 38-39 -0.019648136471154487 1103 40-41 0.1634256251365045 1103 42-43 0.20999709043736914 1103 44-45 0.23471268345145546 1103 46-47 0.09705418332373483 1103 48-49 -0.12059666637708233 1103 50-51 0.017538590609085247 1103 52-53 0.1426161668211492 1103 54-55 0.021481894478661445 1103 56-57 0.030299855411435317 1103 58-59 0.10889712309261057 1103 60-61 -0.012063405752364531 1103 62-63 0.01768663354015132 1103 64-65 -0.03827579563161265 1103 66-67 -0.055522681720198364 1103 68-69 0.07315353577937955 1103 70-71 -0.3440533333386675 1103 72-73 -0.34484092654697207 1103 74-75 -0.2876815255044711 1103 76-77 -0.5283076310163217 1103 78-79 -0.5351566255472768 1103 80-81 -0.4949554836713048 1103 82-83 -0.5735363386548968 1103 84-85 -0.7123009759554435 1103 86-87 -0.9378539032834894 1103 88-89 -0.9509703969590255 1103 90-91 -0.9606445835949451 1103 92-93 -1.078986537612014 1103 94-95 -1.3063260089968018 1103 96-97 -1.3269927330048752 1103 98-99 -1.2595677166515244 1103 100-101 -1.275577277852797 1104 1 0.13997628783152294 1104 2 0.1542190823549845 1104 3 0.19637094976471303 1104 4 0.13380881458966343 1104 5 0.16089767933262777 1104 6 0.16553555073149084 1104 7 0.13096324309520924 1104 8 0.13121935617537162 1104 9 0.14511245597376643 1104 10-11 0.1874813767411041 1104 12-13 0.23291072832506643 1104 14-15 0.23017015516127515 1104 16-17 0.21363458290080217 1104 18-19 0.3466598952050717 1104 20-21 0.28713996170562694 1104 22-23 0.31795409123534313 1104 24-25 0.3520396883274728 1104 26-27 0.27362579407581933 1104 28-29 0.2681417426822179 1104 30-31 0.283425227174142 1104 32-33 0.2201064089295528 1104 34-35 0.1859178763349334 1104 36-37 0.18574424927277278 1104 38-39 0.25904984651867125 1104 40-41 0.24614112137130917 1104 42-43 0.16800539114952784 1104 44-45 0.2165678454568294 1104 46-47 0.07153696091809536 1104 48-49 0.051137286365452894 1104 50-51 0.055912484240487004 1104 52-53 0.1314180659234765 1104 54-55 0.12258236768213493 1104 56-57 0.12190717513034599 1104 58-59 0.07619105948526084 1104 60-61 0.0659888352827025 1104 62-63 0.15691811021797974 1104 64-65 -0.09694948960574123 1104 66-67 0.031341598491721356 1104 68-69 0.13922673296845645 1104 70-71 0.0333040374736413 1104 72-73 -0.14047872937361205 1104 74-75 -0.3012173459072436 1104 76-77 -0.3822414296926091 1104 78-79 -0.420538411143653 1104 80-81 -0.467847291434353 1104 82-83 -0.5242099854658839 1104 84-85 -0.5468967092636241 1104 86-87 -0.4105578445496647 1104 88-89 -0.3227377569238108 1104 90-91 -0.31340598528338504 1104 92-93 -0.44940581790254086 1104 94-95 -0.5519170417122972 1104 96-97 -0.8811568450016019 1104 98-99 -1.0199609000060796 1104 100-101 -1.1511094352519073 1105 1 0.1772510997844492 1105 2 0.2532378766527472 1105 3 0.2815014748094029 1105 4 0.19290125136450342 1105 5 0.189287657293427 1105 6 0.18411570235885222 1105 7 0.15654701438712948 1105 8 0.2133496046146348 1105 9 0.19568366105140456 1105 10-11 0.16501685213065542 1105 12-13 0.22109402552123925 1105 14-15 0.2447246568402477 1105 16-17 0.2300940929225419 1105 18-19 0.3103885214873472 1105 20-21 0.19579287840576143 1105 22-23 0.25342881158130837 1105 24-25 0.1775801098979386 1105 26-27 0.25662311130060544 1105 28-29 0.2209827480983293 1105 30-31 0.2609607025636933 1105 32-33 0.2789746614673305 1105 34-35 0.22446975769797461 1105 36-37 0.2206028533373825 1105 38-39 0.2167195498965313 1105 40-41 0.276166937877818 1105 42-43 0.33766266810294354 1105 44-45 0.32604522478399645 1105 46-47 0.30443464070418713 1105 48-49 0.22709663131621483 1105 50-51 0.14853552160601424 1105 52-53 0.2711974954024825 1105 54-55 0.21884849184525734 1105 56-57 0.12325599836758272 1105 58-59 0.2821126463081356 1105 60-61 0.2645725708836082 1105 62-63 0.3290666949202077 1105 64-65 0.07035701300119257 1105 66-67 -0.009101535299052443 1105 68-69 0.01983208245792767 1105 70-71 -0.055784845070178335 1105 72-73 -0.07594675765280101 1105 74-75 -0.19083467865762316 1105 76-77 -0.3833467360554259 1105 78-79 -0.33416167455232326 1105 80-81 -0.28046681918263516 1105 82-83 -0.39136130647986533 1105 84-85 -0.38774895140342025 1105 86-87 -0.2275182929478774 1105 88-89 -0.23475418074280796 1105 90-91 -0.37661329956366174 1105 92-93 -0.6363323449578253 1105 94-95 -1.0073902600610545 1105 96-97 -0.9998023558144986 1105 98-99 -1.1615724686562778 1105 100-101 -1.253218105493625 1106 1 0.16079505592326626 1106 2 0.11208858985379067 1106 3 0.10085367189252992 1106 4 0.08090593795102308 1106 5 0.06752618712260272 1106 6 0.08559273147099589 1106 7 0.13614572740861064 1106 8 0.0020970158227555658 1106 9 0.10010428298831897 1106 10-11 0.17862187148500652 1106 12-13 0.24651007163487293 1106 14-15 0.16861271478349238 1106 16-17 -0.19201637441597796 1106 18-19 0.1963000617683761 1106 20-21 0.22395004905047955 1106 22-23 0.24240846700404717 1106 24-25 0.17515068958810787 1106 26-27 0.27628982816931824 1106 28-29 0.14391304933449334 1106 30-31 0.11744932994155022 1106 32-33 0.20657786982945225 1106 34-35 0.0864831996270965 1106 36-37 0.06375599852195535 1106 38-39 0.0372296478117633 1106 40-41 -0.05418634638500919 1106 42-43 -0.35059466225863645 1106 44-45 -0.17687140768419596 1106 46-47 -0.06432260904194465 1106 48-49 -0.0254356500585331 1106 50-51 -0.15211309410584306 1106 52-53 -0.17670101784673875 1106 54-55 -0.19021774483692155 1106 56-57 -0.2831966774411683 1106 58-59 -0.12900324152296605 1106 60-61 0.06733644570574171 1106 62-63 -0.1318408544311822 1106 64-65 -0.3466271351059973 1106 66-67 -0.5047751285195972 1106 68-69 -0.4732976749493325 1106 70-71 -0.5243269564831223 1106 72-73 -0.2654595375258708 1106 74-75 -0.4081422580771239 1106 76-77 -0.7266858147337167 1106 78-79 -0.7353098005950471 1106 80-81 -0.7829483008707108 1106 82-83 -0.5072964842203334 1106 84-85 -0.566279104636525 1106 86-87 -0.4546558441559583 1106 88-89 -0.5601707979378823 1106 90-91 -0.7130382420637282 1106 92-93 -0.7709109413033808 1106 94-95 -1.0895828250056319 1106 96-97 -1.077384234948692 1106 98-99 -1.1387262226402335 1106 100-101 -1.4780899242711545 1107 1 -0.008879006086431218 1107 2 0.01631662377935328 1107 3 0.017006325468791772 1107 4 0.06879712576486696 1107 5 0.08020948640069747 1107 6 0.06372082324014627 1107 7 0.07690617253292942 1107 8 0.07399082717011396 1107 9 0.06524476291559722 1107 10-11 0.07541628446428916 1107 12-13 0.033461151447681914 1107 14-15 -0.07407483518956326 1107 16-17 0.016355889652935218 1107 18-19 -2.3522059202463197E-4 1107 20-21 0.08608129935220887 1107 22-23 0.146406323950643 1107 24-25 0.11121976611985218 1107 26-27 0.07098425517321516 1107 28-29 0.06674414719970656 1107 30-31 0.013417296745110718 1107 32-33 0.033798201933940675 1107 34-35 0.12279626120892573 1107 36-37 0.08723131169066534 1107 38-39 -0.0029666790120259634 1107 40-41 0.005850548169604508 1107 42-43 -0.003332883012241439 1107 44-45 -0.134998877835379 1107 46-47 -0.3452279446513131 1107 48-49 -0.2375618879285426 1107 50-51 -0.22050807525413063 1107 52-53 -0.3911736724522328 1107 54-55 -0.4670154340485766 1107 56-57 -0.42035623492560603 1107 58-59 -0.4185129543673156 1107 60-61 -0.4100774223107493 1107 62-63 -0.4865040909895626 1107 64-65 -0.4153763620641868 1107 66-67 -0.37472953003970844 1107 68-69 -0.47009729780046783 1107 70-71 -0.523734253946806 1107 72-73 -0.6494740966337815 1107 74-75 -0.7008011410169068 1107 76-77 -0.8588115405331926 1107 78-79 -0.8673578241492699 1107 80-81 -0.8060617253857174 1107 82-83 -0.9204768358446493 1107 84-85 -0.9030357313960806 1107 86-87 -0.9946344773123172 1107 88-89 -0.8825155052239744 1107 90-91 -0.9418878501323285 1107 92-93 -1.0850197201157847 1107 94-95 -1.3247562494110916 1107 96-97 -1.363877317347086 1107 98-99 -1.5777271577421708 1107 100-101 -1.5942708308933096 1108 1 -0.0024610829621209973 1108 2 -0.0013566992499818298 1108 3 0.04134262204097894 1108 4 0.10785515957532255 1108 5 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-0.311794723828168 2316 94-95 -0.21373047479771046 2316 96-97 -0.2606067317818592 2316 98-99 -0.39462532724420285 2316 100-101 -0.13122773539657118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6656.0 3 1323.0 4 1084.0 5 1150.0 6 1381.0 7 1644.0 8 2164.0 9 2951.0 10 3643.0 11 5284.0 12 7474.0 13 10250.0 14 12873.0 15 17158.0 16 21528.0 17 28865.0 18 27970.0 19 22550.0 20 22499.0 21 23933.0 22 26634.0 23 30475.0 24 34620.0 25 39788.0 26 46055.0 27 54220.0 28 64265.0 29 76938.0 30 93437.0 31 114965.0 32 143402.0 33 183850.0 34 243218.0 35 326256.0 36 417579.0 37 445186.0 38 229718.0 39 14104.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38317416300473 18.600437826903203 14.876875897796632 23.139512112295428 2 15.324886117266159 19.730359616742412 40.63488488051446 24.309869385476972 3 19.596160967649638 23.510184452504067 29.76170514570471 27.13194943414159 4 12.645616498013506 16.666185157166485 37.45230826737545 33.235890077444566 5 13.693979725418032 37.13162917586865 34.423890372785635 14.750500725927681 6 32.0745612594954 36.22573240005653 17.281122273441728 14.418584067006343 7 28.43078034078831 32.772857389045065 20.34364979660953 18.4527124735571 8 26.336941716303176 34.457461018306184 19.541021100966162 19.66457616442448 9 25.719853600462795 17.25519413774139 20.44076075830452 36.58419150349129 10-11 24.80088903041235 26.385397108511665 27.2867474313165 21.526966429759483 12-13 26.151283107783907 23.73441711214685 27.516940732852802 22.59735904721644 14-15 22.585963477961858 25.335853078590798 26.297861426852126 25.78032201659522 16-17 21.917618143220235 28.473295907409806 27.047574749147806 22.56151120022215 18-19 21.877029175542802 26.986775192540392 29.38355865114276 21.75263698077405 20-21 22.350769733437062 25.962804391505912 29.765243696903482 21.921182178153543 22-23 22.050166639810865 25.93046434843093 29.782782057754076 22.236586954004128 24-25 22.062540158492183 26.08849146855144 29.758192332405226 22.09077604055115 26-27 21.551914814069605 26.316492644551086 29.80819038404733 22.323402157331977 28-29 21.616668265730958 27.11908416059646 29.39571776527139 21.868529808401185 30-31 21.7967721507252 26.366640660545958 29.278832919469934 22.55775426925891 32-33 21.54085501526603 26.36253379150142 29.438708426801085 22.657902766431466 34-35 22.46454465570107 26.510458905388056 29.11293314476349 21.912063294147387 36-37 22.391505284240157 25.931843685963628 29.38185487213449 22.294796157661725 38-39 21.817587272113286 26.596817493716156 29.207491960870446 22.37810327330011 40-41 22.423063392392827 26.44986489467987 29.059031811145537 22.068039901781763 42-43 21.966994339844913 26.447889556489002 28.83729518050065 22.74782092316543 44-45 22.05979595229538 26.6366233502889 28.962464979805308 22.341115717610407 46-47 22.64863097248223 27.019183047564226 28.27373956711839 22.05844641283515 48-49 22.245717635696554 26.987956100977016 28.28072851422941 22.485597749097018 50-51 22.203371647127216 27.229388410911966 28.42319414361738 22.14404579834344 52-53 22.674595421658886 27.020771558819195 28.01535200048318 22.28928101903874 54-55 22.073744462304465 27.08693157315104 28.34797577848505 22.491348186059444 56-57 22.33632478419294 27.150498745386507 28.108277313409648 22.404899157010906 58-59 22.49676737810485 26.84148961497941 27.983350036995162 22.678392969920576 60-61 22.011353316058123 27.122626254815106 28.46644706492779 22.39957336419898 62-63 21.997319053963217 27.123282886497112 28.572105758010586 22.30729230152909 64-65 21.72446180061521 27.336075863318733 28.318971193100328 22.620491142965733 66-67 21.834327012512162 27.090414708114906 28.17577608541483 22.8994821939581 68-69 22.303620932604293 26.792188575520786 28.354894099689627 22.549296392185298 70-71 22.300060020843343 26.89954920212197 27.906270538605092 22.89412023842959 72-73 22.177571763929325 26.944345787722295 28.160653972590875 22.717428475757504 74-75 22.028217010392495 27.6109982761736 27.80077242533501 22.5600122880989 76-77 22.2220994931845 27.003905871420507 27.85078373971689 22.9232108956781 78-79 22.391341760587544 27.241799389922573 27.799979975264822 22.566878874225065 80-81 22.659602215756156 27.21520385569507 27.408508840006185 22.71668508854259 82-83 22.463026314054794 27.295821199398684 27.68626873893426 22.55488374761226 84-85 22.392338564856253 27.520562421968975 27.57338479342801 22.513714219746767 86-87 22.600183006088802 27.441898417367906 27.274695132380106 22.68322344416319 88-89 22.463126743764512 27.543414322546933 26.95876419117309 23.03469474251547 90-91 22.473220566248507 27.323393924913276 27.120373944758807 23.083011564079406 92-93 22.727843152913646 27.03888841155354 27.385528943218763 22.847739492314048 94-95 22.430186238951396 26.917788489989324 27.484023776641596 23.16800149441768 96-97 22.516201004204714 26.614098398230922 27.653575574416255 23.21612502314811 98-99 22.455503659791905 26.862206054695854 26.994779058063372 23.68751122744887 100-101 23.192862993668413 25.651683944500014 27.214098954555695 23.94135410727588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 690.0 1 463.5 2 362.5 3 479.0 4 394.0 5 405.5 6 773.5 7 1695.0 8 2860.5 9 3359.5 10 2916.0 11 2364.0 12 2310.5 13 2557.5 14 2800.0 15 3023.5 16 3315.5 17 3669.0 18 4028.0 19 4432.5 20 5024.5 21 6043.5 22 7499.5 23 9284.0 24 11541.0 25 14486.0 26 18581.0 27 23708.5 28 27697.5 29 31002.0 30 35273.0 31 41717.5 32 50087.5 33 53700.0 34 54260.0 35 57232.5 36 62004.5 37 66694.0 38 70483.5 39 73897.5 40 78356.5 41 83574.5 42 86924.5 43 89766.0 44 93139.5 45 97613.0 46 100199.5 47 105614.0 48 113252.5 49 110887.5 50 102862.0 51 97259.5 52 93902.5 53 93438.5 54 94198.0 55 88880.5 56 80644.0 57 73798.5 58 68840.0 59 61786.0 60 50170.0 61 41569.5 62 35149.5 63 26699.5 64 19065.0 65 14373.5 66 10714.0 67 7981.0 68 6122.5 69 4727.0 70 3646.0 71 2602.0 72 1796.0 73 1380.0 74 1041.5 75 733.0 76 532.0 77 362.0 78 235.0 79 154.0 80 109.0 81 69.0 82 45.0 83 32.5 84 18.5 85 9.5 86 9.5 87 9.0 88 5.0 89 2.5 90 4.0 91 8.0 92 5.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1318090507219039 2 0.0482349877506643 3 0.14580930935263586 4 0.1214068229352023 5 0.18378456558764927 6 0.18549452084025775 7 0.1551071910386945 8 0.06640326230962944 9 0.11602758870303813 10-11 0.09573968211219373 12-13 0.14021633071389564 14-15 0.12942223818180457 16-17 0.12815758377622957 18-19 0.12320583835721749 20-21 0.12781915513248412 22-23 0.18130869287814325 24-25 0.2027365696373933 26-27 0.14246064698294425 28-29 0.11998186022469524 30-31 0.09344192974150108 32-33 0.11814722073491739 34-35 0.08804488347545555 36-37 0.08152567907488571 38-39 0.04572349097339559 40-41 0.053507349779540456 42-43 0.05708756858968947 44-45 0.03674622589720106 46-47 0.048395296055596344 48-49 0.03940021894552047 50-51 0.04987369486774743 52-53 0.024277802180264194 54-55 0.047059393514495965 56-57 0.0231200199779772 58-59 0.046988145378970614 60-61 0.03386067640842424 62-63 0.048626852496053745 64-65 0.03720933877811585 66-67 0.03637217318569295 68-69 0.06284085553336176 70-71 0.04983807079998475 72-73 0.047967807242444226 74-75 0.040041452165248646 76-77 0.019486365066184175 78-79 0.020020726082624323 80-81 0.022977523706926496 82-83 0.020234470489200386 84-85 0.01834639489777852 86-87 0.02233629048719831 88-89 0.06191462977153217 90-91 0.10450320278181219 92-93 0.07723297890948316 94-95 0.07071377450891332 96-97 0.063642397058022 98-99 0.052260507407846775 100-101 0.12292084581511607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2807091.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.012062430946656 #Duplication Level Percentage of deduplicated Percentage of total 1 74.32235031544178 38.65658724621445 2 14.275221400512253 14.849674133980582 3 4.85161559321142 7.570275993752001 4 2.2444182488571913 4.669472883228648 5 1.2076282246952685 3.140561730811179 6 0.7109929582752571 2.2188126080265675 7 0.45762603767993065 1.6661451829284708 8 0.3238669491870859 1.347599038435115 9 0.2437351074375987 1.1409449062192087 >10 1.223849697863522 11.556106618842072 >50 0.0861841636688265 3.0658962915928982 >100 0.045175794857176495 4.333961006942363 >500 0.003633476080173467 1.350348344043396 >1k 0.003427807622731523 3.267436497389803 >5k 2.0566845736389139E-4 0.7526221942773051 >10k+ 6.855615245463046E-5 0.4135553233158755 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11598 0.4131679379115248 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9166 0.326530205112695 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6011 0.21413627132144986 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5930 0.21125072183267304 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4008 0.14278126359280835 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3726 0.1327352764837335 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3432 0.12226180056150654 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3294 0.11734567921025717 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3102 0.11050585819982323 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3074 0.10950838430246827 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2907 0.10355916498610127 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.562406776267673E-5 0.0 4 0.0 0.0 0.0 3.562406776267673E-5 0.0 5 0.0 0.0 0.0 3.562406776267673E-5 0.0 6 0.0 0.0 0.0 3.562406776267673E-5 0.0 7 0.0 0.0 0.0 3.562406776267673E-5 0.0 8 0.0 0.0 0.0 3.562406776267673E-5 0.0 9 0.0 0.0 0.0 3.562406776267673E-5 0.0 10-11 0.0 0.0 0.0 7.124813552535346E-5 0.0 12-13 0.0 0.0 0.0 7.124813552535346E-5 0.0 14-15 0.0 0.0 0.0 7.124813552535346E-5 0.0 16-17 0.0 0.0 0.0 7.124813552535346E-5 0.0 18-19 0.0 0.0 0.0 7.124813552535346E-5 0.0 20-21 0.0 0.0 0.0 1.068722032880302E-4 0.0 22-23 0.0 0.0 0.0 1.9593237269472204E-4 0.0 24-25 0.0 0.0 0.0 4.453008470334592E-4 3.562406776267673E-5 26-27 0.0 0.0 0.0 8.371655924229032E-4 3.562406776267673E-5 28-29 0.0 0.0 0.0 0.0024046245739806796 3.562406776267673E-5 30-31 0.0 0.0 0.0 0.006946693213721963 3.562406776267673E-5 32-33 0.0 0.0 0.0 0.018097026423439782 3.562406776267673E-5 34-35 0.0 0.0 0.0 0.03428816522157636 3.562406776267673E-5 36-37 0.0 0.0 0.0 0.06168307333107477 3.562406776267673E-5 38-39 0.0 0.0 0.0 0.10323854837623718 3.562406776267673E-5 40-41 0.0 0.0 0.0 0.1477330090118204 3.562406776267673E-5 42-43 0.0 0.0 0.0 0.19529113947499388 3.562406776267673E-5 44-45 0.0 0.0 0.0 0.25615485924752707 3.562406776267673E-5 46-47 0.0 0.0 0.0 0.331214770023487 3.562406776267673E-5 48-49 0.0 0.0 0.0 0.40671998164648027 3.562406776267673E-5 50-51 0.0 0.0 0.0 0.4863575851299441 3.562406776267673E-5 52-53 0.0 0.0 0.0 0.5631630752262752 3.562406776267673E-5 54-55 0.0 0.0 0.0 0.6478771083659205 3.562406776267673E-5 56-57 0.0 0.0 0.0 0.7524693713171393 3.562406776267673E-5 58-59 0.0 0.0 0.0 0.8617640112130316 3.562406776267673E-5 60-61 0.0 0.0 0.0 0.9769722463575281 3.562406776267673E-5 62-63 0.0 0.0 1.7812033881338366E-5 1.0917529926888725 3.562406776267673E-5 64-65 0.0 0.0 3.562406776267673E-5 1.2113251761342971 3.562406776267673E-5 66-67 0.0 0.0 3.562406776267673E-5 1.3523074243050903 3.562406776267673E-5 68-69 0.0 0.0 3.562406776267673E-5 1.5066665099207686 3.562406776267673E-5 70-71 0.0 0.0 3.562406776267673E-5 1.6714100112892671 3.562406776267673E-5 72-73 0.0 0.0 3.562406776267673E-5 1.8387362575705597 7.124813552535346E-5 74-75 0.0 0.0 3.562406776267673E-5 2.0112279936774407 7.124813552535346E-5 76-77 0.0 0.0 3.562406776267673E-5 2.19408633350326 7.124813552535346E-5 78-79 0.0 0.0 3.562406776267673E-5 2.4084185371974045 7.124813552535346E-5 80-81 0.0 0.0 3.562406776267673E-5 2.633170780712132 8.906016940669184E-5 82-83 0.0 0.0 3.562406776267673E-5 2.8584930093110623 1.0687220328803021E-4 84-85 0.0 0.0 3.562406776267673E-5 3.094751826713135 1.2468423716936858E-4 86-87 0.0 0.0 3.562406776267673E-5 3.333824945468458 1.4249627105070693E-4 88-89 0.0 0.0 3.562406776267673E-5 3.5969977460652327 1.4249627105070693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16630 0.0 24.939262 1 GGTATCA 12480 0.0 24.189804 1 TAAGGTG 1675 0.0 23.849483 5 GTACATG 8720 0.0 23.753654 1 TACATGG 8565 0.0 23.375946 2 GAGTACT 10695 0.0 22.48724 12-13 ACATGGG 8640 0.0 21.907047 3 AGAGTAC 14365 0.0 21.589436 10-11 GTAAGGT 1595 0.0 21.46774 4 GTACTTT 11135 0.0 21.363886 14-15 TATCAAC 19435 0.0 21.190825 2 ATCAACG 19370 0.0 21.163727 3 TCAACGC 19485 0.0 20.990005 4 CAACGCA 19615 0.0 20.802402 5 AACGCAG 20060 0.0 20.336575 6 AGTACTT 11165 0.0 20.306078 12-13 GTAGGAC 695 0.0 19.843931 3 GGTAAGG 1775 0.0 19.022802 3 TATAGGG 625 0.0 19.022802 2 AGGTAAG 1645 0.0 18.791523 2 >>END_MODULE