FastQCFastQC Report
Thu 26 May 2016
SRR937952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807091
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114900.40932053859315565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96940.34533971289138826No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69490.24755164688284062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61670.21969362589242744No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33440.119126882598391No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30610.10904527142155349No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30570.10890277515050277No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30370.10819029379524923No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30140.1073709402367077No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28740.10238357074993294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG18700.025.143035
GTATCAA191150.024.0809761
GTAAGGT18850.023.935164
GGTATCA145150.023.3344251
TATACCG1652.4353449E-623.0266155
GAGTACT118550.022.75468412-13
GGTAAGG19200.022.2628563
AGTACTT122550.021.60506612-13
AGAGTAC158800.021.59231410-11
GTACTTT124900.021.33165214-15
GTACATG87050.021.2823941
TACATGG85000.021.0672742
TATCAAC220950.020.8312682
TCAACGC221250.020.7571264
ATCAACG220650.020.7497393
CAACGCA221400.020.7430655
ACATGGG84150.020.2618853
AACGCAG227400.020.2368036
AGGTAAG19250.019.9866622
AAGGTAA19250.019.49491
Produced by FastQC (version 0.11.5)