##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937952_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2807091 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00873466517473 31.0 31.0 34.0 30.0 34.0 2 32.28245040862587 33.0 31.0 34.0 30.0 34.0 3 32.3452567088135 34.0 31.0 34.0 30.0 34.0 4 35.94579904962112 37.0 35.0 37.0 35.0 37.0 5 35.89782625500919 37.0 35.0 37.0 35.0 37.0 6 35.93565260264096 37.0 35.0 37.0 35.0 37.0 7 35.88678101279937 37.0 35.0 37.0 35.0 37.0 8 35.86592490232771 37.0 35.0 37.0 35.0 37.0 9 37.449541536059925 39.0 37.0 39.0 34.0 39.0 10-11 37.38672063000451 39.0 37.0 39.0 34.0 39.0 12-13 37.28163996108427 39.0 37.0 39.0 34.0 39.0 14-15 38.33008940572286 40.0 38.0 41.0 33.5 41.0 16-17 38.14362715708184 40.0 38.0 41.0 33.0 41.0 18-19 38.4624433265612 40.0 38.0 41.0 34.0 41.0 20-21 38.422521749383975 40.0 38.0 41.0 34.0 41.0 22-23 38.274832201734824 40.0 38.0 41.0 34.0 41.0 24-25 38.11343754085635 40.0 37.5 41.0 33.0 41.0 26-27 37.8474994219995 40.0 37.0 41.0 33.0 41.0 28-29 37.68041524125866 39.5 37.0 41.0 32.5 41.0 30-31 37.34391225649614 39.0 36.5 40.5 32.0 41.0 32-33 37.04336874009428 39.0 36.0 40.0 31.0 41.0 34-35 36.80300888001137 39.0 35.0 40.0 31.0 41.0 36-37 36.73820282277988 39.0 35.0 40.0 30.5 41.0 38-39 36.49971750114264 39.0 35.0 40.0 30.0 41.0 40-41 36.2491406584254 38.0 35.0 40.0 30.0 41.0 42-43 35.90607411729795 38.0 35.0 40.0 29.5 41.0 44-45 35.513475516112585 38.0 34.5 40.0 28.0 41.0 46-47 34.975005975937364 38.0 34.0 40.0 26.5 41.0 48-49 34.41095693014583 38.0 33.0 40.0 24.0 41.0 50-51 33.84631990911588 37.5 33.0 40.0 23.5 41.0 52-53 33.08820358869734 37.0 31.5 39.0 20.5 40.0 54-55 32.58035631905058 36.0 31.0 39.0 18.5 40.0 56-57 32.056727053023934 36.0 30.5 39.0 15.5 40.0 58-59 31.421218442864873 35.0 29.5 38.5 11.0 40.0 60-61 30.669684203326504 34.5 28.0 38.0 8.0 40.0 62-63 29.998471549372642 34.0 28.0 38.0 4.5 40.0 64-65 29.28689362047757 34.0 26.5 37.5 2.0 39.0 66-67 28.485615713918786 33.0 26.0 37.0 2.0 39.0 68-69 27.632759500849815 32.5 24.5 36.0 2.0 39.0 70-71 26.732091870195873 31.5 22.5 35.5 2.0 38.5 72-73 25.894691158925735 31.0 20.5 35.0 2.0 38.0 74-75 25.09127153341306 30.5 18.5 34.5 2.0 37.0 76-77 24.05471714312076 29.5 18.0 33.5 2.0 35.5 78-79 24.590706713818683 30.5 18.0 34.0 2.0 36.0 80-81 24.042789492752462 30.0 10.5 34.0 2.0 36.0 82-83 23.180224652496122 29.5 6.5 34.0 2.0 35.0 84-85 22.258467039365662 29.0 2.0 34.0 2.0 35.0 86-87 21.264096176433185 28.0 2.0 33.5 2.0 35.0 88-89 20.124049594402177 26.0 2.0 33.0 2.0 35.0 90-91 19.20546893563479 25.5 2.0 32.0 2.0 34.0 92-93 18.010100313812416 23.5 2.0 32.0 2.0 34.0 94-95 16.776471977573937 19.0 2.0 31.5 2.0 34.0 96-97 15.35579893918651 4.5 2.0 31.0 2.0 34.0 98-99 13.702814586345793 2.0 2.0 30.5 2.0 34.0 100-101 10.690339750296658 2.0 2.0 24.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21155468207744832 1101 2 0.1479695428487915 1101 3 0.1845808018577486 1101 4 0.10616447195515377 1101 5 0.11203158218660292 1101 6 -0.014219971468847348 1101 7 0.03192931587319947 1101 8 0.05303124083776112 1101 9 -0.03165653979842631 1101 10-11 0.057580656252739004 1101 12-13 0.2123077182027302 1101 14-15 0.18692447821270974 1101 16-17 0.27418990663019116 1101 18-19 0.3032132734504316 1101 20-21 0.2338750094708999 1101 22-23 0.2805518657066628 1101 24-25 0.3329900729758961 1101 26-27 0.28486017245041495 1101 28-29 0.36106505912333375 1101 30-31 0.2715709283910783 1101 32-33 0.19627920750425432 1101 34-35 0.10975409766972177 1101 36-37 0.3828358483978036 1101 38-39 0.2851352262480873 1101 40-41 0.14465017890713483 1101 42-43 0.22160866321510042 1101 44-45 0.22140256043276452 1101 46-47 0.13849553773964374 1101 48-49 0.19350030767095205 1101 50-51 0.15888420931469227 1101 52-53 -0.07969337816518163 1101 54-55 -0.08618960550003862 1101 56-57 0.2795320929334508 1101 58-59 0.15801134367386283 1101 60-61 0.14363473438531216 1101 62-63 0.17518673827144937 1101 64-65 0.11392474356804527 1101 66-67 -0.11882608849110099 1101 68-69 -0.10295771279381327 1101 70-71 -0.00555190703871844 1101 72-73 -0.11694900798818963 1101 74-75 -0.5203756006060622 1101 76-77 -0.6387278298447647 1101 78-79 -0.7101597298423563 1101 80-81 -0.7797131490136913 1101 82-83 -0.8562580755643516 1101 84-85 -0.8797388357845612 1101 86-87 -0.998698112782801 1101 88-89 -0.9855501422623902 1101 90-91 -1.0275566350123455 1101 92-93 -0.9949054709489253 1101 94-95 -1.143693792679791 1101 96-97 -1.285335290884598 1101 98-99 -1.300359483137525 1101 100-101 -1.2959684969470704 1102 1 0.13519619797797588 1102 2 0.15838897573658528 1102 3 0.1285527662776218 1102 4 0.08034924531227006 1102 5 0.1326831960709285 1102 6 0.06924798462569726 1102 7 0.19376006059098927 1102 8 0.11658382343854612 1102 9 0.16000513348447498 1102 10-11 0.21409890877269788 1102 12-13 0.2166217464334963 1102 14-15 0.2973939442384079 1102 16-17 0.29030286094300095 1102 18-19 0.31372789271626544 1102 20-21 0.2449338650100401 1102 22-23 0.17181901911476416 1102 24-25 0.2459862479304391 1102 26-27 0.2293698476988979 1102 28-29 0.11406328817437839 1102 30-31 0.1285582185270968 1102 32-33 0.02270131468950609 1102 34-35 0.09327195923042808 1102 36-37 0.1887498305532631 1102 38-39 0.2923507367194347 1102 40-41 0.28051249088921537 1102 42-43 0.21052973484065518 1102 44-45 0.10942763175870596 1102 46-47 0.032292223040713 1102 48-49 0.13682050783754818 1102 50-51 0.18638186582890626 1102 52-53 0.1360551300614148 1102 54-55 0.2573678229527161 1102 56-57 0.3230479429549007 1102 58-59 0.3855891354860752 1102 60-61 0.014489871215374706 1102 62-63 0.228121642137463 1102 64-65 -0.05345872901260407 1102 66-67 -0.028333278396292627 1102 68-69 -0.16442463559836895 1102 70-71 -0.08848456388203729 1102 72-73 -0.24727816844149686 1102 74-75 -0.21873096157096583 1102 76-77 -0.5150600844937223 1102 78-79 -0.37631159566559447 1102 80-81 -0.7027534019285788 1102 82-83 -0.41254010698635213 1102 84-85 -0.6049857671232637 1102 86-87 -0.9317975514810719 1102 88-89 -1.0986561716598047 1102 90-91 -1.3675589728238364 1102 92-93 -1.3515819513169838 1102 94-95 -1.2955159238105889 1102 96-97 -1.3442177120175067 1102 98-99 -1.2621990372056562 1102 100-101 -1.2895285213919543 1103 1 0.15752643184666937 1103 2 0.15005564240324532 1103 3 0.18290610524634587 1103 4 0.13130726165490358 1103 5 0.14399624199936056 1103 6 0.10390915678686952 1103 7 0.11567468442869 1103 8 0.09687052391755202 1103 9 0.19840327380569533 1103 10-11 0.17882312686229795 1103 12-13 0.17552214091082163 1103 14-15 0.24625101665316862 1103 16-17 0.29743691278859075 1103 18-19 0.30034736298958364 1103 20-21 0.28600705464092613 1103 22-23 0.2994553251164547 1103 24-25 0.20620567593832817 1103 26-27 0.27668775415141766 1103 28-29 0.38022100865902075 1103 30-31 0.3482697569886426 1103 32-33 0.35634635554608707 1103 34-35 0.16850441907827474 1103 36-37 0.2444556631821797 1103 38-39 0.33659031124746974 1103 40-41 0.3413958383110227 1103 42-43 0.3912179568750318 1103 44-45 0.24340903497088107 1103 46-47 0.30979997170231144 1103 48-49 0.20680324945490014 1103 50-51 0.0830147201540683 1103 52-53 -0.08188654364948178 1103 54-55 0.00878019263431895 1103 56-57 0.086648605113254 1103 58-59 0.12930039217809863 1103 60-61 0.0177707249769945 1103 62-63 -0.18689843394030703 1103 64-65 -0.3057304957074436 1103 66-67 -0.36011159623230427 1103 68-69 -0.18648894948960404 1103 70-71 -0.4001621491173175 1103 72-73 -0.8745428908985318 1103 74-75 -0.7447946414808015 1103 76-77 -0.8453555902699925 1103 78-79 -0.9583311786467164 1103 80-81 -0.989670491249516 1103 82-83 -1.039415635304188 1103 84-85 -1.238570931958428 1103 86-87 -1.2690897464655713 1103 88-89 -1.5012959997803996 1103 90-91 -1.7287029570447423 1103 92-93 -1.4883299789303983 1103 94-95 -1.3973051602152093 1103 96-97 -1.5846850971964308 1103 98-99 -1.758854785303713 1103 100-101 -1.7462011756607634 1104 1 0.2591983650636678 1104 2 0.2232756269966245 1104 3 0.2449193423668774 1104 4 0.13788494720254363 1104 5 0.13449051167818027 1104 6 0.14352304790290304 1104 7 0.10874956376966338 1104 8 0.13612833334703822 1104 9 0.12952308389545664 1104 10-11 0.1295431507582947 1104 12-13 0.17076534548878897 1104 14-15 0.3157022735507766 1104 16-17 0.2353038026941121 1104 18-19 0.23481496977478855 1104 20-21 0.2575472454944219 1104 22-23 0.17409059685473238 1104 24-25 0.23204753793899613 1104 26-27 0.2242851853325405 1104 28-29 0.11784854564278646 1104 30-31 0.035427234704059174 1104 32-33 0.22187717665631368 1104 34-35 0.1575311291350232 1104 36-37 0.01128582872478745 1104 38-39 0.08487318002389799 1104 40-41 0.21539833795730345 1104 42-43 0.29147853320856854 1104 44-45 0.212002315795921 1104 46-47 0.22106252888760736 1104 48-49 0.11808309979054599 1104 50-51 0.16224446751685662 1104 52-53 0.05747816889119406 1104 54-55 -0.06823838964177753 1104 56-57 -0.07786899459748042 1104 58-59 -0.08756593176977034 1104 60-61 -0.3836198015208261 1104 62-63 -0.3922486988399285 1104 64-65 -0.3370723655686305 1104 66-67 -0.7361774831350978 1104 68-69 -0.7082739956308757 1104 70-71 -0.6254184021632696 1104 72-73 -0.8086956837423109 1104 74-75 -0.9696134564283696 1104 76-77 -1.0576486620051249 1104 78-79 -1.124785218169425 1104 80-81 -0.8883357744641529 1104 82-83 -0.8846851726338123 1104 84-85 -1.3412608028886446 1104 86-87 -1.089059404847248 1104 88-89 -1.3070035254450083 1104 90-91 -1.226958707017232 1104 92-93 -1.1295977240608046 1104 94-95 -1.1852262877286126 1104 96-97 -1.679240112996082 1104 98-99 -1.7187586511088657 1104 100-101 -1.6693417689730286 1105 1 0.22553949985973532 1105 2 0.16800436035196498 1105 3 0.11805114039137976 1105 4 0.016873047220691717 1105 5 0.15111434911747068 1105 6 0.18233703521474354 1105 7 0.21343728219128622 1105 8 0.1780110050580319 1105 9 0.2593774347798501 1105 10-11 0.22757077560994787 1105 12-13 0.14891091429959147 1105 14-15 0.16768453808669648 1105 16-17 0.18750720285888178 1105 18-19 0.21733082997305075 1105 20-21 0.23312120175507545 1105 22-23 0.27252234818347887 1105 24-25 0.17904004877270552 1105 26-27 0.2777916052552669 1105 28-29 0.1585102869482995 1105 30-31 0.3370431457620242 1105 32-33 0.2503370495367889 1105 34-35 0.23661648719033934 1105 36-37 0.249227467953979 1105 38-39 0.2691068937640537 1105 40-41 0.3974496943648802 1105 42-43 0.23702035267741905 1105 44-45 0.053774345336194074 1105 46-47 0.15102856293089673 1105 48-49 0.26203540468706166 1105 50-51 0.040905028044370795 1105 52-53 -0.026926638689580784 1105 54-55 -0.016024282170157278 1105 56-57 -0.022444553979909188 1105 58-59 0.13864600152370699 1105 60-61 -0.09354993634366693 1105 62-63 -0.046664793706668206 1105 64-65 -0.05048349046044365 1105 66-67 -0.149533435654142 1105 68-69 -0.11440697740763284 1105 70-71 -0.280673979629146 1105 72-73 -0.567800984156623 1105 74-75 -0.5307811774673361 1105 76-77 -0.4270306719450758 1105 78-79 -0.5353186056341457 1105 80-81 -0.47942893100795203 1105 82-83 -0.7438260897146414 1105 84-85 -0.9557523991398966 1105 86-87 -1.118094795470622 1105 88-89 -1.25127124975565 1105 90-91 -1.3720501503919351 1105 92-93 -1.4038185444189644 1105 94-95 -1.3120413249513732 1105 96-97 -1.4609300234413567 1105 98-99 -1.599548775997702 1105 100-101 -1.6323956118551983 1106 1 0.21022721260417399 1106 2 0.19400901878284316 1106 3 0.2286273828189067 1106 4 0.205007063833051 1106 5 0.21741889702416017 1106 6 0.16942820398471525 1106 7 0.21251823949378945 1106 8 0.1546568285800589 1106 9 0.11289663785827742 1106 10-11 0.17298787357592005 1106 12-13 0.17953389509505513 1106 14-15 0.2035754795928142 1106 16-17 0.3045255760098726 1106 18-19 0.36681690979642667 1106 20-21 0.4945677988666759 1106 22-23 0.40832319163092734 1106 24-25 0.40220718943697165 1106 26-27 0.3840209218142121 1106 28-29 0.333046323117145 1106 30-31 0.32168132051135956 1106 32-33 0.44042927786205155 1106 34-35 0.47986218741189646 1106 36-37 0.49195607384854867 1106 38-39 0.3515904446262965 1106 40-41 0.3423016209147747 1106 42-43 0.3772972793667648 1106 44-45 0.23787978078313898 1106 46-47 0.06498770590498992 1106 48-49 0.18784176504689043 1106 50-51 0.08296424679964787 1106 52-53 -0.10043139473554419 1106 54-55 -0.28879336578630443 1106 56-57 -0.37828666403819255 1106 58-59 -0.13510866241876585 1106 60-61 -0.40971317479513303 1106 62-63 -0.45703688115809626 1106 64-65 -0.38162480071252247 1106 66-67 -0.4481860553109769 1106 68-69 -0.7848969679436202 1106 70-71 -1.2686953471062452 1106 72-73 -1.4592941815141387 1106 74-75 -1.2765100194974437 1106 76-77 -1.4980459113198386 1106 78-79 -1.5430085493148908 1106 80-81 -1.65078909029452 1106 82-83 -1.6240834588566422 1106 84-85 -1.655663708863397 1106 86-87 -1.8952564250661332 1106 88-89 -1.8400374933704988 1106 90-91 -2.0601291838140128 1106 92-93 -1.7989003910148007 1106 94-95 -1.8289646595251483 1106 96-97 -1.94238063406023 1106 98-99 -1.7859530798799685 1106 100-101 -1.921836023756324 1107 1 -0.05269820559631455 1107 2 0.01686802576598012 1107 3 -0.033291090711564664 1107 4 -0.014491970684709088 1107 5 -0.0983185718009949 1107 6 -0.048531689173422876 1107 7 -0.05897901016518148 1107 8 -0.03657565768416049 1107 9 -0.007732531233401119 1107 10-11 0.05843922362694798 1107 12-13 -0.10719637022244655 1107 14-15 -0.0832865610897855 1107 16-17 -0.05887820778375641 1107 18-19 -0.01156926806962133 1107 20-21 -0.05554174219201968 1107 22-23 -0.06692794266262325 1107 24-25 -0.14372018749700288 1107 26-27 -0.06233510401401787 1107 28-29 -0.06076190341062215 1107 30-31 -0.2956139764265018 1107 32-33 -0.30854158137574217 1107 34-35 -0.08615376753405002 1107 36-37 -0.20701848579945192 1107 38-39 -0.22393269847793107 1107 40-41 -0.34448406775830875 1107 42-43 -0.31195348119172905 1107 44-45 -0.3270179624252094 1107 46-47 -0.3432819912001932 1107 48-49 -0.5208202901825914 1107 50-51 -0.64025557883906 1107 52-53 -0.7145230663159197 1107 54-55 -0.5575207214731748 1107 56-57 -0.5887219864088813 1107 58-59 -0.7013337128328736 1107 60-61 -0.7803013591369599 1107 62-63 -0.8720901116544155 1107 64-65 -1.081203527162529 1107 66-67 -1.227277680397389 1107 68-69 -1.1882295153378486 1107 70-71 -1.5553319875872553 1107 72-73 -1.9045495741454914 1107 74-75 -1.8541059018389205 1107 76-77 -1.7351504912812459 1107 78-79 -1.7357219002547843 1107 80-81 -1.519182504659831 1107 82-83 -1.84580324901982 1107 84-85 -1.892030881779295 1107 86-87 -2.0813661042055394 1107 88-89 -2.164465830946245 1107 90-91 -2.3827702423682595 1107 92-93 -2.4426196739479096 1107 94-95 -2.2936472694187344 1107 96-97 -2.424082838039835 1107 98-99 -2.5212476433595814 1107 100-101 -2.3657803502249823 1108 1 -0.1438649167462991 1108 2 -0.07977820098420096 1108 3 -0.09401104368698299 1108 4 0.016993142418328944 1108 5 -0.06935309690628344 1108 6 -0.004851424057328302 1108 7 -0.0476080880921117 1108 8 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2316 100-101 3.588127271657479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 31.0 5 77.0 6 165.0 7 465.0 8 1189.0 9 2263.0 10 3833.0 11 6364.0 12 9562.0 13 14347.0 14 19599.0 15 26511.0 16 34716.0 17 45210.0 18 47324.0 19 43464.0 20 44846.0 21 49413.0 22 54092.0 23 59394.0 24 66207.0 25 73747.0 26 83271.0 27 95634.0 28 112477.0 29 130190.0 30 149861.0 31 171777.0 32 197586.0 33 227485.0 34 259535.0 35 284781.0 36 271807.0 37 177780.0 38 41335.0 39 734.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.36189637445251 19.821448038664528 15.611914009478026 22.20474157740494 2 16.145754469097096 20.91382458515556 39.313340043288115 23.627080902459227 3 20.168486065840387 23.9973650449485 30.327634358303296 25.506514530907815 4 13.506019937379968 17.705209916750057 36.996440084372104 31.79233006149787 5 13.589085270080641 37.50577559725109 34.46188889549918 14.443250237169089 6 31.845399042267207 37.06216428230959 16.885580072432017 14.206856602991186 7 28.318747058787906 33.05575059732656 20.2801761681399 18.345326175745637 8 26.258999084817695 35.01126254902317 19.257444806741216 19.472293559417917 9 25.614630947126404 17.25270039339658 20.34647255824624 36.78619610123078 10-11 24.87633995477881 26.744875745032846 27.122134622639592 21.25664967754875 12-13 26.283236361013117 23.955633493613007 27.48197632943217 22.279153815941697 14-15 22.528909821591107 25.57439356258846 26.290829189363652 25.605867426456786 16-17 21.7895086528365 28.72132531654927 27.13290126299083 22.3562647676234 18-19 21.78443805348669 27.162176074804844 29.568688724376948 21.484697147331524 20-21 22.33769763787494 26.005248850144152 30.028542003091456 21.628511508889453 22-23 22.072102400670303 26.00603257963493 30.023964310383956 21.897900709310814 24-25 22.211425814039774 26.190615722424287 29.913108409020513 21.68485005451543 26-27 21.660804302993938 26.434313497736056 30.038491358239355 21.866390841030654 28-29 21.701485098338267 27.097135167978692 29.67198727002583 21.52939246365721 30-31 21.907365422542437 26.450476806621143 29.491437145886295 22.150720624950125 32-33 21.649600957004957 26.47484531139176 29.621590465004523 22.253963266598767 34-35 22.5292269555104 26.504438485651004 29.371956907864792 21.594377650973797 36-37 22.463428136609053 26.031803200198354 29.583042995629615 21.92172566756297 38-39 21.902525845015052 26.63038502383844 29.417539092154993 22.049550038991512 40-41 22.48393905113266 26.433987527437697 29.2550893885965 21.826984032833145 42-43 22.066516970174348 26.48208255537114 29.0384476309871 22.412952843467416 44-45 22.23426315712601 26.51082561983206 29.238132999607064 22.016778223434866 46-47 22.766649501788063 27.002604299793187 28.409097183790248 21.821649014628502 48-49 22.444300086905457 26.992399735881516 28.337733229999145 22.22556694721389 50-51 22.386103489232905 27.259957149646347 28.49391765806501 21.860021703055743 52-53 22.934090808283038 27.061126283213383 28.057218192723244 21.947564715780338 54-55 22.3639546060521 27.11383115999393 28.29474500996475 22.22746922398922 56-57 22.607075726402208 27.14803036460002 28.104036754484525 22.140857154513245 58-59 22.74390742539851 26.939221861522572 27.884889994525068 22.43198071855385 60-61 22.216854670509218 27.25057316724086 28.353230978159704 22.17934118409022 62-63 22.280916886229765 27.239690570583754 28.399632213625374 22.079760329561108 64-65 21.933099873792173 27.44854590195127 28.11785359749323 22.50050062676333 66-67 22.05463792439093 27.155461360769458 28.02758457534052 22.7623161394991 68-69 22.508941325342015 26.909839119396384 28.15899656886161 22.42222298639999 70-71 22.53190219569768 27.01666592437536 27.700725960896094 22.750705919030864 72-73 22.49041975654345 27.00542956167525 27.87589440989031 22.628256271890987 74-75 22.344731827659384 27.666865719290197 27.47034876014919 22.518053692901233 76-77 22.403620700805284 27.011359407182216 27.89977012526329 22.68524976674921 78-79 22.536883783321993 27.278882148813665 27.835227877079415 22.34900619078493 80-81 22.783549377910617 27.204034130565912 27.522177777793612 22.49023871372986 82-83 22.669517346764955 27.243369300035564 27.761416678010942 22.325696675188535 84-85 22.53832880956432 27.487424159688008 27.61119162934966 22.363055401398018 86-87 22.784319350295196 27.35917553914149 27.280937995276528 22.57556711528679 88-89 22.544972412858073 27.642175465589254 26.84157912937264 22.971272992180037 90-91 22.596833978429945 27.304459469379232 27.178278999593058 22.920427552597765 92-93 22.79489645915263 27.10441420686148 27.29572661389333 22.804962720092558 94-95 22.55169354594477 27.036753322870215 27.29173384259366 23.119819288591344 96-97 22.528231905125836 26.75331754947598 27.469452179009608 23.24899836638858 98-99 22.480899793927268 27.064628598790925 26.80639637790832 23.64807522937349 100-101 23.156361993600544 26.246623825486736 26.88608846868731 23.71092571222541 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 738.0 1 487.5 2 346.5 3 478.5 4 411.5 5 376.5 6 741.5 7 1707.5 8 2813.0 9 3274.5 10 2830.5 11 2235.5 12 2188.0 13 2461.5 14 2786.5 15 3086.0 16 3420.5 17 3752.0 18 4137.5 19 4692.5 20 5365.5 21 6284.0 22 7703.0 23 9648.0 24 12124.0 25 15203.5 26 19328.0 27 24215.5 28 28255.5 29 32029.5 30 36353.5 31 42898.0 32 50940.0 33 54589.5 34 55249.5 35 57698.0 36 62183.0 37 66886.0 38 71004.5 39 74500.0 40 78646.5 41 83790.0 42 87300.5 43 90276.0 44 93381.5 45 97342.5 46 99888.0 47 105769.5 48 112503.5 49 109386.0 50 102218.0 51 96691.5 52 93257.0 53 92253.0 54 92451.5 55 87429.5 56 79404.0 57 72677.0 58 66897.5 59 59690.0 60 49483.5 61 41153.0 62 34693.5 63 27061.0 64 19808.5 65 14813.0 66 10930.0 67 8174.0 68 6404.0 69 4969.0 70 3771.5 71 2740.5 72 1927.5 73 1419.0 74 1079.0 75 782.0 76 558.0 77 386.5 78 241.5 79 154.0 80 101.5 81 56.0 82 30.0 83 16.0 84 10.5 85 9.0 86 6.5 87 3.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0063054599939937825 2 0.01125720541300585 3 0.007552302365687468 4 7.124813552535348E-5 5 7.837294907788882E-4 6 0.0016030830493204533 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.590452536095196E-4 14-15 0.0 16-17 0.0022621283029299727 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0031171059292342145 26-27 0.01734892100042357 28-29 0.006145151689061736 30-31 0.002956797624302169 32-33 0.0 34-35 0.007890731009432897 36-37 5.34361016440151E-4 38-39 0.0024936847433873715 40-41 0.0017633913542524984 42-43 0.002778677285488785 44-45 0.0 46-47 8.906016940669183E-5 48-49 6.234211858468429E-4 50-51 0.004025519657182471 52-53 0.007890731009432897 54-55 0.007017941349247317 56-57 0.016654251679051376 58-59 0.023600944892773337 60-61 0.003580218810149012 62-63 0.020323530658607078 64-65 0.021695057267470133 66-67 0.013448085580410466 68-69 0.03133136759727419 70-71 0.01660081557740736 72-73 0.005076429656181435 74-75 0.01649394337411933 76-77 0.020038538116505664 78-79 0.026450870313787473 80-81 0.01211218303931009 82-83 0.03480471420413517 84-85 0.02203348591121556 86-87 0.031830104545951664 88-89 0.022104734046740914 90-91 0.028249885735802654 92-93 0.024455922519077578 94-95 0.014463371511646755 96-97 0.015175852866900288 98-99 0.01122158134524317 100-101 0.010330979651176254 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2807091.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.26210482375011 #Duplication Level Percentage of deduplicated Percentage of total 1 74.57838419233941 38.97623332245951 2 14.036448974991163 14.67148735368416 3 4.930460349510899 7.730287068464467 4 2.2464848592436564 4.696241087950379 5 1.1628038879447486 3.038528934061631 6 0.6973925475028995 2.18683214525392 7 0.42829468301349694 1.566850713337434 8 0.31975633128727055 1.336891112303448 9 0.24017495356626245 1.129684373938739 >10 1.2214419495302264 11.565725648692105 >50 0.0885458939840491 3.1640419056226032 >100 0.04284841086160804 4.352840937676808 >500 0.0032084256132603206 1.199975683360734 >1k 0.0034814831121979866 3.1606792244554183 >5k 2.047931242469404E-4 0.8137786632107288 >10k+ 6.826437474898013E-5 0.40992182552789447 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11490 0.40932053859315565 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9694 0.34533971289138826 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6949 0.24755164688284062 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6167 0.21969362589242744 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3344 0.119126882598391 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3061 0.10904527142155349 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3057 0.10890277515050277 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3037 0.10819029379524923 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3014 0.1073709402367077 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2874 0.10238357074993294 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.124813552535346E-5 0.0 10-11 0.0 0.0 0.0 1.0687220328803021E-4 0.0 12-13 0.0 0.0 0.0 1.0687220328803021E-4 0.0 14-15 0.0 0.0 0.0 1.0687220328803021E-4 0.0 16-17 0.0 0.0 0.0 1.0687220328803021E-4 3.562406776267673E-5 18-19 0.0 0.0 0.0 1.0687220328803021E-4 3.562406776267673E-5 20-21 0.0 0.0 0.0 1.4249627105070696E-4 3.562406776267673E-5 22-23 0.0 0.0 0.0 2.3155644045739877E-4 3.562406776267673E-5 24-25 0.0 0.0 0.0 4.8092491479613596E-4 3.562406776267673E-5 26-27 0.0 0.0 0.0 8.727896601855799E-4 3.562406776267673E-5 28-29 0.0 0.0 0.0 0.002422436607862018 3.562406776267673E-5 30-31 0.0 0.0 0.0 0.006946693213721963 3.562406776267673E-5 32-33 0.0 0.0 0.0 0.01800796625403309 3.562406776267673E-5 34-35 0.0 0.0 0.0 0.03387848844230558 3.562406776267673E-5 36-37 0.0 0.0 0.0 0.06079247163700785 3.562406776267673E-5 38-39 0.0 0.0 0.0 0.10197389397066214 3.562406776267673E-5 40-41 0.0 0.0 0.0 0.1462724222335507 3.562406776267673E-5 42-43 0.0 0.0 0.0 0.1930646352398266 3.562406776267673E-5 44-45 0.0 0.0 0.0 0.25271713670842877 3.562406776267673E-5 46-47 0.0 0.0 0.0 0.3261739644350682 3.562406776267673E-5 48-49 0.0 0.0 0.0 0.3996307921617076 3.562406776267673E-5 50-51 0.0 0.0 0.0 0.47759406446032565 3.562406776267673E-5 52-53 0.0 0.0 0.0 0.5528320955750989 3.562406776267673E-5 54-55 0.0 0.0 0.0 0.6352305643101702 3.562406776267673E-5 56-57 0.0 0.0 0.0 0.7365454130272229 3.562406776267673E-5 58-59 0.0 0.0 0.0 0.841369232418899 5.34361016440151E-5 60-61 0.0 0.0 0.0 0.9521244590930611 7.124813552535346E-5 62-63 0.0 0.0 0.0 1.0620247081409189 1.0687220328803021E-4 64-65 0.0 0.0 0.0 1.1763245295574671 1.0687220328803021E-4 66-67 1.7812033881338366E-5 0.0 0.0 1.3113753704457747 1.0687220328803021E-4 68-69 3.562406776267673E-5 0.0 0.0 1.4578437250520202 1.0687220328803021E-4 70-71 3.562406776267673E-5 0.0 0.0 1.6138593298186628 1.0687220328803021E-4 72-73 3.562406776267673E-5 0.0 0.0 1.7720658147527102 1.0687220328803021E-4 74-75 3.562406776267673E-5 0.0 0.0 1.9362927671386498 1.0687220328803021E-4 76-77 3.562406776267673E-5 0.0 0.0 2.1093723003636144 1.0687220328803021E-4 78-79 3.562406776267673E-5 0.0 0.0 2.309543937122095 1.0687220328803021E-4 80-81 3.562406776267673E-5 0.0 0.0 2.5187997111600584 1.2468423716936858E-4 82-83 3.562406776267673E-5 0.0 0.0 2.727147071470074 1.781203388133837E-4 84-85 3.562406776267673E-5 0.0 0.0 2.9440442080431306 1.781203388133837E-4 86-87 3.562406776267673E-5 0.0 0.0 3.1595341939395625 1.9593237269472204E-4 88-89 3.562406776267673E-5 0.0 0.0 3.393709003377518 2.1374440657606042E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 1870 0.0 25.14303 5 GTATCAA 19115 0.0 24.080976 1 GTAAGGT 1885 0.0 23.93516 4 GGTATCA 14515 0.0 23.334425 1 TATACCG 165 2.4353449E-6 23.026615 5 GAGTACT 11855 0.0 22.754684 12-13 GGTAAGG 1920 0.0 22.262856 3 AGTACTT 12255 0.0 21.605066 12-13 AGAGTAC 15880 0.0 21.592314 10-11 GTACTTT 12490 0.0 21.331652 14-15 GTACATG 8705 0.0 21.282394 1 TACATGG 8500 0.0 21.067274 2 TATCAAC 22095 0.0 20.831268 2 TCAACGC 22125 0.0 20.757126 4 ATCAACG 22065 0.0 20.749739 3 CAACGCA 22140 0.0 20.743065 5 ACATGGG 8415 0.0 20.261885 3 AACGCAG 22740 0.0 20.236803 6 AGGTAAG 1925 0.0 19.986662 2 AAGGTAA 1925 0.0 19.4949 1 >>END_MODULE