FastQCFastQC Report
Thu 26 May 2016
SRR937951_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937951_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913429
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103690.35590364481166353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84350.28952138528174187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51370.1763214411609138No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36750.12614002263312407No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35920.123291145931478No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30750.10554573322363442No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30390.10431007585906503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.054.0479741
GTATCAA85800.045.249411
ATCAACG106550.036.2185673
TATCAAC107000.036.0218282
TCAACGC107600.035.90934
CAACGCA110100.035.093925
AACGCAG113600.034.1329156
ACGCAGA129900.029.6588237
CGCAGAG129900.029.5125398
TGGTATC21150.029.2120272
GTGGTAT23550.027.138741
GCAGAGT147050.025.8754149
GTACATG83000.024.5946961
TACATGG82450.024.5554332
GAGTACT97700.022.8515512-13
TAAGGTG16500.022.7547365
ACATGGG84850.022.5726153
TATACCG1703.1369655E-622.3650445
CAGAGTA139100.022.06062110-11
GTAAGGT15950.022.049554
Produced by FastQC (version 0.11.5)