##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937951_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2913429 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05236853206308 33.0 31.0 34.0 31.0 34.0 2 32.22097912803092 34.0 31.0 34.0 31.0 34.0 3 32.31636947390858 34.0 31.0 34.0 30.0 34.0 4 35.842336985044085 37.0 35.0 37.0 35.0 37.0 5 35.84873082542941 37.0 35.0 37.0 35.0 37.0 6 35.88482918238268 37.0 36.0 37.0 35.0 37.0 7 35.82553307460041 37.0 36.0 37.0 35.0 37.0 8 35.79329649014958 37.0 36.0 37.0 35.0 37.0 9 37.45984885851002 39.0 37.0 39.0 35.0 39.0 10-11 37.35816781531316 39.0 37.0 39.0 35.0 39.0 12-13 37.22232256217673 39.0 37.0 39.0 34.0 39.0 14-15 38.41414961545313 40.0 38.0 41.0 34.0 41.0 16-17 38.224028112578 40.0 38.0 41.0 33.5 41.0 18-19 38.40770068534363 40.0 38.0 41.0 34.0 41.0 20-21 38.38898322217565 40.0 38.0 41.0 34.0 41.0 22-23 38.276512144280844 40.0 38.0 41.0 34.0 41.0 24-25 38.138314336817544 40.0 38.0 41.0 33.5 41.0 26-27 37.92889220914599 40.0 38.0 41.0 33.0 41.0 28-29 37.71671799793302 40.0 38.0 41.0 33.0 41.0 30-31 37.46585775730248 40.0 37.0 41.0 32.0 41.0 32-33 37.14583176730925 39.5 36.5 41.0 31.5 41.0 34-35 36.83542348208932 39.0 36.0 41.0 30.5 41.0 36-37 36.51961142694742 39.0 35.5 40.5 30.0 41.0 38-39 36.17490764319295 39.0 35.0 40.0 30.0 41.0 40-41 36.00864067735991 39.0 35.0 40.0 29.5 41.0 42-43 35.726679627339465 39.0 35.0 40.0 27.5 41.0 44-45 35.36035355589582 38.0 35.0 40.0 26.5 41.0 46-47 34.89505390383634 38.0 34.0 40.0 25.0 41.0 48-49 34.400068441688475 38.0 33.5 40.0 23.5 41.0 50-51 33.551575651920814 37.5 32.5 39.5 22.0 40.5 52-53 33.51519292215461 37.5 33.0 39.5 21.5 40.5 54-55 33.467412454533815 38.0 33.0 40.0 19.0 41.0 56-57 32.95163087894024 37.0 32.0 40.0 14.0 41.0 58-59 32.44498235584255 37.0 31.0 40.0 9.0 41.0 60-61 31.841399601637796 36.0 31.0 39.0 6.0 41.0 62-63 31.18024911538946 35.5 30.0 39.0 2.0 40.5 64-65 30.530373659354666 35.0 29.0 39.0 2.0 40.0 66-67 29.798304334857654 35.0 28.0 38.0 2.0 40.0 68-69 29.04487598633775 34.0 27.0 37.5 2.0 40.0 70-71 28.16379307681773 34.0 26.0 37.0 2.0 39.0 72-73 27.32147170910978 33.5 24.0 36.0 2.0 39.0 74-75 26.450949551198946 33.0 21.0 35.5 2.0 38.5 76-77 25.58147873176247 32.0 18.0 35.0 2.0 37.5 78-79 24.713975696679068 31.5 9.0 35.0 2.0 37.0 80-81 23.88231736555104 31.0 4.0 35.0 2.0 36.0 82-83 23.023001761841456 30.5 2.0 34.5 2.0 36.0 84-85 22.196151510814232 29.5 2.0 34.0 2.0 35.0 86-87 21.397618579344133 29.0 2.0 34.0 2.0 35.0 88-89 20.533957237330995 28.0 2.0 34.0 2.0 35.0 90-91 19.65597325350987 26.5 2.0 34.0 2.0 35.0 92-93 18.73777377104436 25.0 2.0 33.5 2.0 35.0 94-95 17.785221984129354 22.5 2.0 33.0 2.0 35.0 96-97 16.665873614905323 12.5 2.0 32.5 2.0 34.5 98-99 15.337594120193078 2.0 2.0 32.0 2.0 34.0 100-101 12.34114406083004 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5542748134080853 1101 2 0.5197541260799952 1101 3 0.48893912090007774 1101 4 0.3069464021666377 1101 5 0.33756850790155113 1101 6 0.3380744996704905 1101 7 0.30631126831261213 1101 8 0.37275747415915816 1101 9 0.477017100892283 1101 10-11 0.5117029661878405 1101 12-13 0.5555276994719378 1101 14-15 0.6945992946656503 1101 16-17 0.8063107766843203 1101 18-19 0.7701603674265414 1101 20-21 0.7638893667540287 1101 22-23 0.7669135923505763 1101 24-25 0.7328649274538677 1101 26-27 1.0118858095173735 1101 28-29 0.959211292032343 1101 30-31 1.0685424979604718 1101 32-33 1.140447945775044 1101 34-35 1.1445903381918754 1101 36-37 1.2221117394025711 1101 38-39 1.3295931676349753 1101 40-41 1.4959472239506226 1101 42-43 1.532029893309172 1101 44-45 1.691098948011465 1101 46-47 1.8975781585317435 1101 48-49 1.9755670268848888 1101 50-51 2.2521228228162684 1101 52-53 2.282188049193799 1101 54-55 2.4525985059946365 1101 56-57 2.6680483997226574 1101 58-59 2.8991240316849556 1101 60-61 2.8955709407456602 1101 62-63 3.1562232889586284 1101 64-65 3.2028184569760576 1101 66-67 3.359256093178331 1101 68-69 3.7434878880959097 1101 70-71 3.967266042849463 1101 72-73 4.3447278037393 1101 74-75 4.471272915707921 1101 76-77 4.7726465244050615 1101 78-79 5.066945726955154 1101 80-81 5.240877442347848 1101 82-83 5.525815708268073 1101 84-85 5.745654681757628 1101 86-87 5.848411151995851 1101 88-89 5.904336233074265 1101 90-91 5.9720327439103755 1101 92-93 6.191847160245835 1101 94-95 6.045075297393229 1101 96-97 6.045567575533102 1101 98-99 6.025368802517521 1101 100-101 4.957252941533705 1102 1 0.3859443504266622 1102 2 0.36717775195033653 1102 3 0.31848696768823714 1102 4 0.21324098313631623 1102 5 0.20714768594324795 1102 6 0.1576300504981134 1102 7 0.29103588249476786 1102 8 0.2993392657514491 1102 9 0.3996718479467205 1102 10-11 0.3855349027907593 1102 12-13 0.4986994905939355 1102 14-15 0.6124331382422952 1102 16-17 0.667097915641456 1102 18-19 0.5501378151947236 1102 20-21 0.482158214216291 1102 22-23 0.5591031464344951 1102 24-25 0.549851718609446 1102 26-27 0.6055930481791094 1102 28-29 0.6737314188354517 1102 30-31 0.7438390698868602 1102 32-33 0.7523327423906423 1102 34-35 1.0118573245911193 1102 36-37 1.0092457975277611 1102 38-39 1.1443574889740447 1102 40-41 1.252362824406589 1102 42-43 1.3742707889933428 1102 44-45 1.456112166933842 1102 46-47 1.467733627839884 1102 48-49 1.64585849264558 1102 50-51 1.9250586329385655 1102 52-53 1.9331754651828064 1102 54-55 2.0368226655488755 1102 56-57 2.3562601555493927 1102 58-59 2.51324207593391 1102 60-61 2.7188952281780026 1102 62-63 2.790517266370557 1102 64-65 2.851957127446969 1102 66-67 3.0471771278935478 1102 68-69 3.3659532815058206 1102 70-71 3.556571313173908 1102 72-73 3.7215132820728094 1102 74-75 3.911361411382238 1102 76-77 4.190269850624105 1102 78-79 4.40673137997328 1102 80-81 4.810031554490891 1102 82-83 5.192460772643837 1102 84-85 5.250794580619715 1102 86-87 5.351997505544077 1102 88-89 5.161486684678788 1102 90-91 5.3951874696891196 1102 92-93 5.4334901530412125 1102 94-95 5.479787342121451 1102 96-97 5.432066106390689 1102 98-99 5.51841529102081 1102 100-101 4.525856265244455 1103 1 0.508255541943953 1103 2 0.35263606174418527 1103 3 0.42794886310431934 1103 4 0.2625696858573221 1103 5 0.278460367659946 1103 6 0.2451980428871039 1103 7 0.329452330638766 1103 8 0.29793914231199636 1103 9 0.3472557982051896 1103 10-11 0.37954798040630777 1103 12-13 0.5068749052427322 1103 14-15 0.6020294999351421 1103 16-17 0.6756403014191434 1103 18-19 0.605371936515894 1103 20-21 0.6573004580494839 1103 22-23 0.7516484211743659 1103 24-25 0.7387203426731759 1103 26-27 0.7801566015372785 1103 28-29 0.8332782741228328 1103 30-31 0.8418920683008722 1103 32-33 0.8706150072760366 1103 34-35 0.912052607113985 1103 36-37 1.0169007581283651 1103 38-39 1.2705094272673776 1103 40-41 1.3793576941583723 1103 42-43 1.427770063487202 1103 44-45 1.4280330927515266 1103 46-47 1.7104727155853112 1103 48-49 1.9423432711675517 1103 50-51 1.9970798124929061 1103 52-53 2.0814513948157938 1103 54-55 2.225131512369657 1103 56-57 2.4779855811379363 1103 58-59 2.6441436846743827 1103 60-61 2.906753014823682 1103 62-63 2.827058175011217 1103 64-65 3.062962260960777 1103 66-67 3.3423899575416556 1103 68-69 3.4573372565828073 1103 70-71 3.6919273282348293 1103 72-73 3.914985577317342 1103 74-75 4.104549463879927 1103 76-77 4.351000619813007 1103 78-79 4.514547552065732 1103 80-81 4.612638913615886 1103 82-83 4.95323301487689 1103 84-85 5.19096985049385 1103 86-87 5.059654724847221 1103 88-89 5.241496187557413 1103 90-91 5.4536041845233 1103 92-93 5.508817882615194 1103 94-95 5.512636496713583 1103 96-97 5.297181017201876 1103 98-99 5.256170538014013 1103 100-101 4.387619860184527 1104 1 0.4184331865177242 1104 2 0.38554485923185666 1104 3 0.2933352758802741 1104 4 0.18384102948004255 1104 5 0.24897184612056833 1104 6 0.26571917933051026 1104 7 0.2713159474598541 1104 8 0.3590741733733154 1104 9 0.304584649403715 1104 10-11 0.43909152129280926 1104 12-13 0.40883002366206256 1104 14-15 0.6583321644998961 1104 16-17 0.7595120932553385 1104 18-19 0.626088569223846 1104 20-21 0.7132181014186045 1104 22-23 0.5742964427417832 1104 24-25 0.6668569023958568 1104 26-27 0.740336894267621 1104 28-29 0.8130624545547178 1104 30-31 0.9464493380618322 1104 32-33 0.9257784452686693 1104 34-35 0.972840262342686 1104 36-37 1.0369192640695815 1104 38-39 1.1967582667517078 1104 40-41 1.4140212848094507 1104 42-43 1.4079200529984277 1104 44-45 1.518015635688002 1104 46-47 1.8353455002205976 1104 48-49 1.922952793730886 1104 50-51 1.8626217395616322 1104 52-53 2.078160670840937 1104 54-55 2.092256362047827 1104 56-57 2.355517686142022 1104 58-59 2.705915311805633 1104 60-61 2.7323757989397315 1104 62-63 2.8460997188761112 1104 64-65 3.0851218793769952 1104 66-67 3.269171024572202 1104 68-69 3.368481478305167 1104 70-71 3.6311996459637683 1104 72-73 3.914615354643722 1104 74-75 4.0451030220677175 1104 76-77 4.1250835268450565 1104 78-79 4.350669238494913 1104 80-81 4.731535172341822 1104 82-83 4.8171842306403825 1104 84-85 4.930316212757514 1104 86-87 5.010693165695709 1104 88-89 5.100924248047537 1104 90-91 5.331409413126714 1104 92-93 5.280011962375593 1104 94-95 5.270418701932218 1104 96-97 5.270066490141868 1104 98-99 5.186635398534046 1104 100-101 4.331980792501797 1105 1 0.4147134079012673 1105 2 0.36714421861947955 1105 3 0.37250611522015475 1105 4 0.28151357456672343 1105 5 0.32888891939060727 1105 6 0.3261035370652152 1105 7 0.27925501366648575 1105 8 0.288780585216891 1105 9 0.4158593012985534 1105 10-11 0.390123888310967 1105 12-13 0.4252872811081687 1105 14-15 0.644532411127912 1105 16-17 0.658428660472623 1105 18-19 0.5528359781270851 1105 20-21 0.647988918655038 1105 22-23 0.6412001075525993 1105 24-25 0.6354502453784505 1105 26-27 0.6872934522557372 1105 28-29 0.7135932767547288 1105 30-31 0.8854824622202315 1105 32-33 0.7743999604604355 1105 34-35 0.9395479044179709 1105 36-37 0.9962424863653823 1105 38-39 1.0351326982906173 1105 40-41 1.1583770272964529 1105 42-43 1.0780463626038355 1105 44-45 1.2620484302334916 1105 46-47 1.4066628272925783 1105 48-49 1.4201559312579946 1105 50-51 1.6175225503124437 1105 52-53 1.5943079467356753 1105 54-55 1.7078002281939675 1105 56-57 2.0266826399525826 1105 58-59 2.2613271195094526 1105 60-61 2.391793082095635 1105 62-63 2.590581005103232 1105 64-65 2.6763383739824924 1105 66-67 2.8403592936487954 1105 68-69 2.715634155973799 1105 70-71 3.1421825419621676 1105 72-73 3.412912516191856 1105 74-75 3.4536023575099257 1105 76-77 3.7766005217835605 1105 78-79 3.949846733107197 1105 80-81 4.106065958672193 1105 82-83 4.290718131812735 1105 84-85 4.413264076720452 1105 86-87 4.5158718449755675 1105 88-89 4.708200646890791 1105 90-91 4.798018292510683 1105 92-93 4.873219706727824 1105 94-95 4.880375750461901 1105 96-97 4.791994715961611 1105 98-99 4.6290675346292804 1105 100-101 3.8035845192604523 1106 1 0.35558364663464914 1106 2 0.2831632789776748 1106 3 0.25210883396939465 1106 4 0.23258054542402817 1106 5 0.29502463541886925 1106 6 0.18742138933013308 1106 7 0.1070531790868614 1106 8 0.11397500935798632 1106 9 0.310046290344701 1106 10-11 0.22450442785499547 1106 12-13 0.27758325578223264 1106 14-15 0.48965787316900133 1106 16-17 0.4650385478378425 1106 18-19 0.43958710171286697 1106 20-21 0.4716798348282012 1106 22-23 0.5556203534102622 1106 24-25 0.5002186327231612 1106 26-27 0.6398192413262649 1106 28-29 0.6455855460198663 1106 30-31 0.7142525409002687 1106 32-33 0.712424694639374 1106 34-35 0.7236348073800372 1106 36-37 0.7845026656127843 1106 38-39 0.7861784029887815 1106 40-41 1.0123509422303982 1106 42-43 0.8697844597780886 1106 44-45 1.0291229985861818 1106 46-47 1.0609500565254137 1106 48-49 1.1944115030746687 1106 50-51 1.3634727931899988 1106 52-53 1.2798668021943058 1106 54-55 1.4839062759150394 1106 56-57 1.4164729039398267 1106 58-59 1.8411052662905263 1106 60-61 2.092951981082326 1106 62-63 2.0148570607119893 1106 64-65 2.2814618350605578 1106 66-67 2.329516634539644 1106 68-69 2.254539610233895 1106 70-71 2.6741376132298846 1106 72-73 3.001893729662129 1106 74-75 3.131163465711527 1106 76-77 3.0278467969458482 1106 78-79 3.456300490828035 1106 80-81 3.6126201201655554 1106 82-83 3.5074610713649257 1106 84-85 3.64376114770414 1106 86-87 3.777582812979361 1106 88-89 3.8491853245875696 1106 90-91 4.160653722026645 1106 92-93 4.214424324400451 1106 94-95 4.177215058669237 1106 96-97 4.199568598083548 1106 98-99 3.9847370523521466 1106 100-101 3.310136369214778 1107 1 0.39020799433262 1107 2 0.34986298850034814 1107 3 0.33603246373878193 1107 4 0.31825622621556704 1107 5 0.2923788358261632 1107 6 0.28288839737266613 1107 7 0.2387332202332928 1107 8 0.22561774527270728 1107 9 0.30143867056244034 1107 10-11 0.3030050425558102 1107 12-13 0.34377604963447084 1107 14-15 0.5255353135107086 1107 16-17 0.5299163389032273 1107 18-19 0.5130205490323974 1107 20-21 0.5412441694737993 1107 22-23 0.4975748620541154 1107 24-25 0.49937528033532885 1107 26-27 0.7156340891267803 1107 28-29 0.6994716041088225 1107 30-31 0.729682152081665 1107 32-33 0.7989296819927034 1107 34-35 0.8991346308125543 1107 36-37 0.9892604269564771 1107 38-39 1.0117071077743347 1107 40-41 1.0655209646036496 1107 42-43 1.0688397363126754 1107 44-45 1.2925018890237894 1107 46-47 1.370002416591916 1107 48-49 1.4772899184257753 1107 50-51 1.5153843095496526 1107 52-53 1.7130172906213303 1107 54-55 1.689080408591309 1107 56-57 1.9434144862039346 1107 58-59 2.007564581199034 1107 60-61 2.2857291350700315 1107 62-63 2.3351173871369895 1107 64-65 2.4740183550425847 1107 66-67 2.6939156980387082 1107 68-69 2.8612883101510285 1107 70-71 3.021211542139195 1107 72-73 3.1321719009172817 1107 74-75 3.287963652924905 1107 76-77 3.4822509109140434 1107 78-79 3.653811630735433 1107 80-81 3.6761698271382777 1107 82-83 3.994706979056236 1107 84-85 3.99372164457192 1107 86-87 4.1628544562409395 1107 88-89 4.511994050939794 1107 90-91 4.452286424519528 1107 92-93 4.340781057758466 1107 94-95 4.2058144703842295 1107 96-97 4.296364654738991 1107 98-99 4.176274436131978 1107 100-101 3.6484992335457225 1108 1 0.40618113375477805 1108 2 0.3503680251110808 1108 3 0.27597525654328336 1108 4 0.20816897173946103 1108 5 0.2074034604623023 1108 6 0.09006827142395935 1108 7 0.14358656906013323 1108 8 0.2063173206734632 1108 9 0.2794411838830655 1108 10-11 0.3311326287862002 1108 12-13 0.3685996338598727 1108 14-15 0.4644254419252931 1108 16-17 0.47759261523119534 1108 18-19 0.3640866785413479 1108 20-21 0.43468144460820923 1108 22-23 0.3723178322397871 1108 24-25 0.38037587118525806 1108 26-27 0.4549178356554435 1108 28-29 0.49964854359705413 1108 30-31 0.47419287609619687 1108 32-33 0.6394387543532716 1108 34-35 0.7078057005436946 1108 36-37 0.8756265585602847 1108 38-39 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2311 50-51 1.2500246708122589 2311 52-53 1.1810482958095534 2311 54-55 1.471964604056943 2311 56-57 1.6244032596104745 2311 58-59 1.736036668404786 2311 60-61 1.8326169574297317 2311 62-63 2.003143239986642 2311 64-65 2.202400496508627 2311 66-67 2.1587928775164613 2311 68-69 2.3579744646943226 2311 70-71 2.9876440012557204 2311 72-73 3.4575630547923133 2311 74-75 3.664487308434264 2311 76-77 3.5864957112906453 2311 78-79 3.9059235941353343 2311 80-81 4.055433965298459 2311 82-83 4.21715583934353 2311 84-85 4.475423081083548 2311 86-87 4.305676841258467 2311 88-89 4.557262409613898 2311 90-91 4.854059346426151 2311 92-93 5.049715433038514 2311 94-95 4.984733566998724 2311 96-97 5.152474920949754 2311 98-99 5.130106785199837 2311 100-101 4.59644773305922 2312 1 -0.20827052026751502 2312 2 -0.20338840384234658 2312 3 -0.17708732660406667 2312 4 -0.06166069156041232 2312 5 -0.08931968611388896 2312 6 -0.039697706895928775 2312 7 -0.0934729824135232 2312 8 0.03144708293317677 2312 9 0.015841979618528512 2312 10-11 -0.05730923923142228 2312 12-13 0.06829012233299636 2312 14-15 0.04410677137234842 2312 16-17 0.04507885021114788 2312 18-19 -0.01769072467192956 2312 20-21 -0.0057808292867918 2312 22-23 0.11378561574872492 2312 24-25 0.11789545863243234 2312 26-27 0.16550861312017418 2312 28-29 0.28848639750923866 2312 30-31 0.23828625984924656 2312 32-33 0.1780067843490869 2312 34-35 0.3310408583126474 2312 36-37 0.35218359862629 2312 38-39 0.46619315842014686 2312 40-41 0.6936113936802784 2312 42-43 0.8217855992169163 2312 44-45 0.9122517679847064 2312 46-47 0.73166090206891 2312 48-49 1.0113883879905856 2312 50-51 1.0425097563745211 2312 52-53 1.1750339085848864 2312 54-55 1.2994630070820747 2312 56-57 1.6193826930908983 2312 58-59 1.703905376918577 2312 60-61 1.8986418653222792 2312 62-63 2.0450574647907587 2312 64-65 2.227375040267308 2312 66-67 2.243001425819614 2312 68-69 2.4306654277925546 2312 70-71 2.9124996247011374 2312 72-73 3.233759734067661 2312 74-75 2.8578443452196396 2312 76-77 2.986371826543376 2312 78-79 3.37163950779075 2312 80-81 3.6864202616910937 2312 82-83 4.148528044293453 2312 84-85 4.452113490524507 2312 86-87 4.48874373685927 2312 88-89 4.825126360135496 2312 90-91 4.99702103731434 2312 92-93 5.051313882483569 2312 94-95 5.264648902263172 2312 96-97 5.145329576674609 2312 98-99 4.942441791498277 2312 100-101 4.291126882180793 2313 1 -0.3664191141405553 2313 2 -0.1904895886022615 2313 3 -0.26201306517117473 2313 4 -0.0341469237745855 2313 5 0.021958041206289636 2313 6 -0.08500679974157777 2313 7 -0.008438321032265605 2313 8 0.04820952256495303 2313 9 0.04950568964341784 2313 10-11 0.010066081772507118 2313 12-13 -0.007041484443490731 2313 14-15 -0.025726307550485217 2313 16-17 0.09985906635491659 2313 18-19 -0.005722143066932972 2313 20-21 -0.03100186348816436 2313 22-23 0.04668573729075831 2313 24-25 0.040200014538974926 2313 26-27 0.0632075149582434 2313 28-29 0.23689340376424894 2313 30-31 0.1556360336776308 2313 32-33 0.2391712859697961 2313 34-35 0.43516875318486825 2313 36-37 0.4502052554532412 2313 38-39 0.44283143376196676 2313 40-41 0.8362743760737601 2313 42-43 0.8344323194053018 2313 44-45 1.1031465250057906 2313 46-47 1.1598008843826975 2313 48-49 1.32030357078893 2313 50-51 1.3763801785531697 2313 52-53 1.485265668162036 2313 54-55 1.602566641592297 2313 56-57 1.832454434152126 2313 58-59 1.9877892480257557 2313 60-61 2.244356558170555 2313 62-63 2.3867994657173313 2313 64-65 2.6055550027530963 2313 66-67 2.748204202666386 2313 68-69 2.87831692071045 2313 70-71 3.1772943626004313 2313 72-73 3.588564465080406 2313 74-75 3.9238914156976215 2313 76-77 3.894482794289292 2313 78-79 4.180552037464743 2313 80-81 4.273295081881621 2313 82-83 4.442775694445341 2313 84-85 4.635049501603184 2313 86-87 4.90979191779039 2313 88-89 4.903038829800639 2313 90-91 4.946645875883782 2313 92-93 4.9171894742991356 2313 94-95 5.089675978603115 2313 96-97 5.151146093541055 2313 98-99 4.8856563922107785 2313 100-101 4.502696472474719 2314 1 -0.27146132061030315 2314 2 -0.20214099205085745 2314 3 -0.10284277101897032 2314 4 -0.11396926290540677 2314 5 -0.17303028695816636 2314 6 -0.10926641526626213 2314 7 -0.17453689341514433 2314 8 -0.0691881055334207 2314 9 -0.05327977276805029 2314 10-11 -0.12869070267700522 2314 12-13 -0.1742456130135963 2314 14-15 -0.12599526394895122 2314 16-17 0.04245315001413985 2314 18-19 0.04317829794474193 2314 20-21 0.01988205174057356 2314 22-23 0.016165623120741657 2314 24-25 0.010496285624469692 2314 26-27 0.1156155605884166 2314 28-29 0.18743018144467527 2314 30-31 0.18674295578839661 2314 32-33 0.23307046038064527 2314 34-35 0.25758393680604286 2314 36-37 0.4431191014341991 2314 38-39 0.5482368869752108 2314 40-41 0.5934909738987599 2314 42-43 0.651228392507555 2314 44-45 0.8133666505837311 2314 46-47 0.9359869345196614 2314 48-49 1.1233074579954305 2314 50-51 1.1239802111688562 2314 52-53 1.3621818630774314 2314 54-55 1.6682099565789983 2314 56-57 1.7721054917685635 2314 58-59 2.085276696001941 2314 60-61 2.30516139172369 2314 62-63 2.4343070241744087 2314 64-65 2.590466230020265 2314 66-67 2.9663808328497794 2314 68-69 3.1809393141755073 2314 70-71 3.603764636452965 2314 72-73 3.8038262579477475 2314 74-75 4.0262052371979 2314 76-77 4.260091289576952 2314 78-79 4.3302629327481625 2314 80-81 4.581423263400371 2314 82-83 4.914004132670691 2314 84-85 5.305783606046386 2314 86-87 5.3245642957138095 2314 88-89 5.559384244620453 2314 90-91 5.499669864111869 2314 92-93 5.81998452844514 2314 94-95 5.922506471086656 2314 96-97 5.750665163593066 2314 98-99 5.870538768599902 2314 100-101 5.137867660341987 2315 1 -0.3820353985467584 2315 2 -0.30484660516374973 2315 3 -0.23744795194416923 2315 4 -0.1317900352875654 2315 5 -0.16437879126875288 2315 6 -0.12462159893335922 2315 7 -0.11852843564955151 2315 8 -0.16527684976886547 2315 9 -0.23702806668516985 2315 10-11 -0.12776034661823132 2315 12-13 -0.06016135221970842 2315 14-15 -0.0964407795719211 2315 16-17 0.03541771537901184 2315 18-19 -0.11286663858928847 2315 20-21 -0.09530942907530715 2315 22-23 0.04984972896741624 2315 24-25 0.050247774705681536 2315 26-27 -4.4839688607822836E-4 2315 28-29 0.09615496646372179 2315 30-31 0.01717705036794115 2315 32-33 0.11871804653777218 2315 34-35 0.32198771861655473 2315 36-37 0.3679434379155353 2315 38-39 0.47153055295042634 2315 40-41 0.5313809858958365 2315 42-43 0.6854041967616382 2315 44-45 0.9246462489154439 2315 46-47 1.0885040386658034 2315 48-49 1.0436216712498663 2315 50-51 1.1768862224883279 2315 52-53 1.4042500488817353 2315 54-55 1.5751897882808166 2315 56-57 1.8233114339553396 2315 58-59 2.078480059974801 2315 60-61 2.1565469535967203 2315 62-63 2.3678637308254764 2315 64-65 2.541150538721329 2315 66-67 2.7133531088935996 2315 68-69 3.0892771459117974 2315 70-71 3.505263298176164 2315 72-73 3.7435593786142256 2315 74-75 4.015295016350201 2315 76-77 4.179038839319826 2315 78-79 4.5085509319798796 2315 80-81 4.677773110089081 2315 82-83 4.809037444200939 2315 84-85 5.022342192764153 2315 86-87 5.313652323737763 2315 88-89 5.5087188481055485 2315 90-91 5.657186301446771 2315 92-93 5.941582604489639 2315 94-95 5.973490296467087 2315 96-97 6.071121927911559 2315 98-99 5.853470520584461 2315 100-101 5.536400279300976 2316 1 -0.7160951329632823 2316 2 -0.6787383713093078 2316 3 -0.6299529665959405 2316 4 -0.5086709600342019 2316 5 -0.47207706098825497 2316 6 -0.4677159875291892 2316 7 -0.5061555744480799 2316 8 -0.5926228777682923 2316 9 -0.6539819162573437 2316 10-11 -0.5781125492594796 2316 12-13 -0.45811283975551476 2316 14-15 -0.5982054215269912 2316 16-17 -0.4101253715473874 2316 18-19 -0.4918683809470039 2316 20-21 -0.4928769205472818 2316 22-23 -0.5659342491945836 2316 24-25 -0.609693564243095 2316 26-27 -0.5300757820673496 2316 28-29 -0.47063694786506005 2316 30-31 -0.36474653264750145 2316 32-33 -0.20997486137965637 2316 34-35 -0.13339472080706116 2316 36-37 -0.014239228463061693 2316 38-39 -0.03750470602034994 2316 40-41 0.11326672757394363 2316 42-43 0.35011264167899725 2316 44-45 0.4091420007670834 2316 46-47 0.29053635202296846 2316 48-49 0.6056343837805684 2316 50-51 0.6559079601826383 2316 52-53 0.807934820027711 2316 54-55 1.2391949072358557 2316 56-57 1.1915825937946494 2316 58-59 1.2621832372514632 2316 60-61 1.3639979194300942 2316 62-63 1.7181976594597188 2316 64-65 2.074530887613438 2316 66-67 2.3900789464972263 2316 68-69 2.49167738648854 2316 70-71 2.7632394277079833 2316 72-73 3.4212046853486413 2316 74-75 3.608146232250892 2316 76-77 3.9335654708937007 2316 78-79 4.288477037627086 2316 80-81 4.40825588441831 2316 82-83 4.486245858878728 2316 84-85 4.702504548570232 2316 86-87 5.050901830739665 2316 88-89 5.316237448234734 2316 90-91 5.450920773425668 2316 92-93 5.617119933801202 2316 94-95 5.912919732468332 2316 96-97 5.980966686314115 2316 98-99 5.795912097262809 2316 100-101 5.447313797283657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6568.0 3 2071.0 4 2287.0 5 2554.0 6 2964.0 7 3823.0 8 4898.0 9 6417.0 10 8318.0 11 10893.0 12 14184.0 13 17996.0 14 22078.0 15 27325.0 16 32574.0 17 40656.0 18 42033.0 19 40868.0 20 44007.0 21 49164.0 22 54681.0 23 62307.0 24 69031.0 25 75805.0 26 82379.0 27 89786.0 28 98229.0 29 107733.0 30 117611.0 31 130973.0 32 148493.0 33 171572.0 34 206356.0 35 257265.0 36 315054.0 37 341798.0 38 189079.0 39 13598.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9425916509224 17.994456089000234 14.12129768583402 23.941654574243344 2 15.732454818294736 19.127991886827562 40.409139815585995 24.730413479291705 3 19.54788179258108 22.89067760729592 29.03575059843012 28.525690001692876 4 12.882141351268576 15.892231984298883 36.110546476381714 35.11508018805083 5 14.127519717286818 36.670770678466994 34.057390913134036 15.144318691112153 6 33.621801355097965 35.2425751738003 16.71869027573486 14.416933195366882 7 29.757860312609974 31.878389397697916 19.841137138099448 18.52261315159266 8 26.637243339839422 35.03326003251329 19.13350534134664 19.195991286300643 9 26.333092078699366 16.59132609477874 19.70718462708992 37.368397199431975 10-11 25.48903514117687 25.97696303297749 26.75135093007231 21.78265089577333 12-13 26.21831125546439 23.266643377111404 27.292177198609835 23.22286816881437 14-15 22.863586372421306 24.828510313756478 25.429292829593127 26.878610484229092 16-17 22.656048022857824 28.052182009762365 26.339544594492985 22.95222537288682 18-19 22.72444501359378 26.43230798333796 28.49624870696862 22.34699829609964 20-21 22.949122832619278 25.732280463507657 28.968352958041727 22.350243745831342 22-23 22.77028581237662 25.56551368981203 28.858415586644924 22.805784911166423 24-25 22.680612918539936 25.768058287183017 28.86439748191403 22.68693131236302 26-27 22.214811144566834 26.197924875365132 28.703069422282745 22.88419455778529 28-29 22.386845715745395 26.559549429164324 28.594709418342273 22.458895436748 30-31 22.421281458402152 26.0596469228503 28.538050871842486 22.981020746905063 32-33 22.191776118416477 26.010611404727836 28.649797033922674 23.147815442933013 34-35 22.953321362911343 26.18763576383029 28.312216851994183 22.54682602126418 36-37 22.969874431877294 25.80536340110347 28.257136706194764 22.967625460824472 38-39 22.575594875593982 26.11402071156438 28.329274463587232 22.981109949254403 40-41 22.95892656817904 25.902261627036815 28.356585576171835 22.782226228612306 42-43 22.61105036118411 25.91658824290672 28.198809766757467 23.273551629151704 44-45 22.681642123785235 26.25939849301439 28.322445696117953 22.736513687082425 46-47 23.04266003520788 26.557457919439308 27.634377669084348 22.76550437626846 48-49 22.93654669208106 26.371694934600278 27.68571401599184 23.00604435732682 50-51 22.957083442606518 26.737100386243522 27.612927830267047 22.69288834088291 52-53 23.20665177304928 26.4419546233801 27.584445077168606 22.766948526402018 54-55 22.741716605507783 26.619569092032147 27.642459350838834 22.99625495162124 56-57 22.852240105751925 26.81378386143504 27.54656081405092 22.78741521876211 58-59 22.852146656270868 26.39371460375687 27.512733165336954 23.241405574635305 60-61 22.66690671608618 26.59796195983303 27.676639701725435 23.05849162235535 62-63 22.641320318618295 26.63930677128234 27.876938137997332 22.842434772102038 64-65 22.489987479522284 26.58218419261798 27.682166943707625 23.245661384152115 66-67 22.493728787030022 26.6760649048231 27.574475373732714 23.255730934414164 68-69 22.85260648150055 26.230007043616876 27.864472847749404 23.052913627133165 70-71 22.6981358032977 26.46230025329814 27.468215005953546 23.37134893745062 72-73 22.791648558672303 26.53070051677298 27.30256450393707 23.37508642061764 74-75 22.762166071723417 26.877898696365428 27.168373345398113 23.191561886513046 76-77 23.027764257140326 26.532021713266523 27.2427934239586 23.197420605634548 78-79 23.032741924189605 26.583376469312125 27.243562899122225 23.140318707376046 80-81 23.14023444693827 26.719241218366147 26.97032280520727 23.170201529488313 82-83 23.085870582159387 26.712980865528348 26.943470554423044 23.25767799788922 84-85 23.086625238388798 26.657720364616445 26.94025142552727 23.315402971467485 86-87 23.331233278866925 26.5817468940675 26.84770612097515 23.239313706090428 88-89 23.25080597083588 26.6918349631273 26.637402642791287 23.419956423245537 90-91 23.231773433556082 26.647428183108207 26.704707643881388 23.416090739454322 92-93 23.275607569160382 26.204067579239048 26.901661606737054 23.61866324486352 94-95 23.154788450440115 26.139611483093923 26.960720194426678 23.744879872039288 96-97 23.553832353275386 25.863145026490574 26.766979422387756 23.816043197846287 98-99 23.274340490779117 26.05062066155266 26.3907018945029 24.284336953165322 100-101 24.047052420277595 24.884152279191802 26.72766525351804 24.341130047012562 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 714.0 1 488.5 2 407.5 3 548.0 4 434.0 5 416.0 6 791.0 7 1687.5 8 2785.0 9 3321.0 10 2857.0 11 2320.5 12 2377.5 13 2551.0 14 2726.0 15 2948.0 16 3153.0 17 3337.0 18 3725.0 19 4228.0 20 4752.0 21 5604.5 22 6825.0 23 8500.0 24 10750.5 25 13615.5 26 17205.0 27 21202.5 28 24730.5 29 28314.5 30 32505.0 31 37817.5 32 45310.0 33 49234.0 34 50406.0 35 53610.5 36 57688.0 37 62552.0 38 67210.5 39 70660.0 40 74472.0 41 80213.5 42 84397.5 43 87251.0 44 90594.0 45 95629.5 46 99029.0 47 100953.5 48 102243.5 49 101413.5 50 101453.5 51 101102.5 52 102218.0 53 105375.5 54 107642.5 55 103406.5 56 95786.0 57 89825.0 58 84931.5 59 76992.0 60 64054.5 61 53546.0 62 45149.5 63 35971.0 64 27779.0 65 21643.5 66 16709.0 67 12836.0 68 10053.0 69 7675.0 70 5919.5 71 4581.5 72 3431.0 73 2650.5 74 2029.5 75 1518.0 76 1089.0 77 697.5 78 429.0 79 280.0 80 177.0 81 110.5 82 68.0 83 40.0 84 29.5 85 18.5 86 13.0 87 13.5 88 10.5 89 7.5 90 6.0 91 3.0 92 2.5 93 2.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3701823521355763 2 0.02179562295837654 3 0.04208099802672384 4 0.035181911074544804 5 0.05642835298200163 6 0.05526134324879721 7 0.029312538592840255 8 0.026566633338241642 9 0.03010198635353736 10-11 0.027664995440081087 12-13 0.02819701458315957 14-15 0.022121699207360124 16-17 0.028008233596905914 18-19 0.02402667097773792 20-21 0.036777968503780255 22-23 0.02330587084840578 24-25 0.044500827032338874 26-27 0.038511321195745636 28-29 0.02941551003988771 30-31 0.03744728290958867 32-33 0.03420368232759405 34-35 0.03337991075121446 36-37 0.03384328226292798 38-39 0.016029221923719437 40-41 0.010468763783157234 42-43 0.01038295424395103 44-45 0.00901000161665172 46-47 0.015274097978704819 48-49 0.013094535682867163 50-51 0.00827203957947834 52-53 0.0034495434760895153 54-55 0.003964400711326756 56-57 0.006727467873766616 58-59 0.0024884766369799986 60-61 0.004805334195547583 62-63 0.005577620048403445 64-65 0.006916248860020272 66-67 0.00401588643485048 68-69 0.005251543799419859 70-71 0.015737469490418335 72-73 0.01204765930455144 74-75 0.009130134971540409 76-77 0.006418553532624272 78-79 0.005526134324879721 80-81 0.007980287146177236 82-83 0.009730801745983855 84-85 0.0041531816975804116 86-87 4.290476960310342E-4 88-89 0.021881432497582745 90-91 0.017762574615684815 92-93 0.011841716410456544 94-95 0.02265371835043861 96-97 0.017367850735336265 98-99 0.015394231333593508 100-101 0.10787975269004324 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2913429.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.684615343232366 #Duplication Level Percentage of deduplicated Percentage of total 1 73.49992731029522 36.51815616167558 2 14.108702284901447 14.01970892035024 3 5.084925874939948 7.579277584357219 4 2.3420876912087882 4.654629041513113 5 1.2849808050761782 3.1921888511823484 6 0.7922020712414265 2.361615311024535 7 0.5440456983843306 1.8921490877355975 8 0.3797273108474518 1.5093284299820537 9 0.2798317115662753 1.2512997855007864 >10 1.5051936655348521 13.636039966274142 >50 0.11477302753874305 3.9568499913705724 >100 0.05754758323108382 5.239216024225792 >500 0.00320147587105076 1.131775630604832 >1k 0.002644697458612586 2.2299057466902883 >5k 1.3919460308487293E-4 0.46930824498085805 >10k+ 6.959730154243647E-5 0.35855122253216304 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10369 0.35590364481166353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8435 0.28952138528174187 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5137 0.1763214411609138 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3675 0.12614002263312407 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3592 0.123291145931478 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3075 0.10554573322363442 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3039 0.10431007585906503 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.0297144704744821E-4 0.0 0.0 0.0 0.0 3 1.0297144704744821E-4 0.0 0.0 0.0 0.0 4 1.0297144704744821E-4 0.0 0.0 0.0 0.0 5 1.0297144704744821E-4 0.0 0.0 0.0 0.0 6 1.0297144704744821E-4 0.0 0.0 0.0 0.0 7 1.0297144704744821E-4 0.0 0.0 0.0 0.0 8 1.0297144704744821E-4 0.0 0.0 0.0 0.0 9 1.0297144704744821E-4 0.0 0.0 0.0 0.0 10-11 1.0297144704744821E-4 0.0 0.0 0.0 0.0 12-13 1.0297144704744821E-4 0.0 0.0 0.0 2.059428940948964E-4 14-15 1.0297144704744821E-4 0.0 0.0 0.0 2.9175243330110327E-4 16-17 1.0297144704744821E-4 0.0 0.0 0.0 3.0891434114234466E-4 18-19 1.0297144704744821E-4 0.0 0.0 0.0 3.0891434114234466E-4 20-21 1.0297144704744821E-4 0.0 0.0 0.0 4.2904769603103424E-4 22-23 1.0297144704744821E-4 0.0 0.0 3.4323815682482736E-5 5.14857235237241E-4 24-25 1.7161907841241369E-4 0.0 0.0 1.2013335488868959E-4 5.491810509197238E-4 26-27 1.7161907841241369E-4 0.0 0.0 4.6337151171351696E-4 6.693144058084134E-4 28-29 2.4026670977737917E-4 0.0 0.0 0.002334019466408826 7.208001293321375E-4 30-31 2.4026670977737917E-4 0.0 0.0 0.009370401681317787 7.551239450146203E-4 32-33 2.4026670977737917E-4 0.0 0.0 0.026806900048019017 8.409334842208272E-4 34-35 2.574286176186205E-4 0.0 0.0 0.05287583805886466 9.439049312682754E-4 36-37 2.745905254598619E-4 1.7161907841241368E-5 0.0 0.09330929293282933 9.610668391095167E-4 38-39 2.745905254598619E-4 3.4323815682482736E-5 0.0 0.15385650379672888 9.78228746950758E-4 40-41 2.745905254598619E-4 3.4323815682482736E-5 0.0 0.21781893432103544 9.953906547919993E-4 42-43 3.4323815682482737E-4 3.4323815682482736E-5 0.0 0.2840124128647034 9.953906547919993E-4 44-45 3.4323815682482737E-4 3.4323815682482736E-5 0.0 0.36215057926587535 0.0010468763783157233 46-47 3.4323815682482737E-4 3.4323815682482736E-5 0.0 0.45652391048486163 0.001115524009680689 48-49 3.4323815682482737E-4 3.4323815682482736E-5 0.0 0.5550504234014284 0.0011498478253631718 50-51 3.4323815682482737E-4 3.4323815682482736E-5 0.0 0.6634450333267088 0.0012356573645693786 52-53 3.4323815682482737E-4 3.4323815682482736E-5 0.0 0.7638250322901297 0.0012699811802518613 54-55 3.4323815682482737E-4 3.4323815682482736E-5 0.0 0.8725972041879174 0.0013386288116168268 56-57 3.4323815682482737E-4 3.4323815682482736E-5 0.0 0.9969523884055523 0.0013386288116168268 58-59 3.4323815682482737E-4 3.4323815682482736E-5 0.0 1.1287386787184448 0.0013386288116168268 60-61 3.4323815682482737E-4 3.4323815682482736E-5 0.0 1.2706161708419872 0.0013729526272993095 62-63 3.4323815682482737E-4 3.4323815682482736E-5 0.0 1.4091299290286463 0.0014759240743467577 64-65 3.4323815682482737E-4 3.4323815682482736E-5 0.0 1.550818640165935 0.0014759240743467577 66-67 3.4323815682482737E-4 3.4323815682482736E-5 0.0 1.7158818697829945 0.0014759240743467577 68-69 3.4323815682482737E-4 3.4323815682482736E-5 0.0 1.9000806266430381 0.0014759240743467577 70-71 3.4323815682482737E-4 3.4323815682482736E-5 0.0 2.0903546988788815 0.0014930859821879992 72-73 3.6040006466606876E-4 3.4323815682482736E-5 0.0 2.2842842574849085 0.0015788955213942059 74-75 3.7756197250731014E-4 3.4323815682482736E-5 0.0 2.4786943495104907 0.0016475431527591714 76-77 3.7756197250731014E-4 3.4323815682482736E-5 0.0 2.684448462619134 0.0016990288762828956 78-79 3.7756197250731014E-4 3.4323815682482736E-5 0.0 2.917009475775796 0.001716190784124137 80-81 4.4620960387227557E-4 3.4323815682482736E-5 0.0 3.1508061462970267 0.0017505145998066196 82-83 4.4620960387227557E-4 3.4323815682482736E-5 0.0 3.3946082090897014 0.0018878098625365505 84-85 4.4620960387227557E-4 3.4323815682482736E-5 0.0 3.6454466540972854 0.0018878098625365505 86-87 4.6337151171351696E-4 3.4323815682482736E-5 0.0 3.903836338555015 0.0019221336782190334 88-89 4.8053341955475834E-4 3.4323815682482736E-5 0.0 4.174977320538788 0.0019907813095839987 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4730 0.0 54.047974 1 GTATCAA 8580 0.0 45.24941 1 ATCAACG 10655 0.0 36.218567 3 TATCAAC 10700 0.0 36.021828 2 TCAACGC 10760 0.0 35.9093 4 CAACGCA 11010 0.0 35.09392 5 AACGCAG 11360 0.0 34.132915 6 ACGCAGA 12990 0.0 29.658823 7 CGCAGAG 12990 0.0 29.512539 8 TGGTATC 2115 0.0 29.212027 2 GTGGTAT 2355 0.0 27.13874 1 GCAGAGT 14705 0.0 25.875414 9 GTACATG 8300 0.0 24.594696 1 TACATGG 8245 0.0 24.555433 2 GAGTACT 9770 0.0 22.85155 12-13 TAAGGTG 1650 0.0 22.754736 5 ACATGGG 8485 0.0 22.572615 3 TATACCG 170 3.1369655E-6 22.365044 5 CAGAGTA 13910 0.0 22.060621 10-11 GTAAGGT 1595 0.0 22.04955 4 >>END_MODULE