FastQCFastQC Report
Thu 26 May 2016
SRR937951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913429
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101710.34910752930653194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87000.2986171964375998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53440.18342647100718776No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34930.1198930881789122No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33750.11584287792837925No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31130.10685003821956876No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30650.10520249506680958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50450.053.7345121
GTATCAA88650.042.3289871
ATCAACG111750.033.3203123
TCAACGC113850.032.6616944
TATCAAC115250.032.4355472
CAACGCA116750.031.84935
AACGCAG120850.030.6935636
TGGTATC22000.028.7520352
GTGGTAT24550.027.3153761
CGCAGAG137100.026.8823498
ACGCAGA137750.026.7899767
GTACATG86850.025.0804461
TACATGG85000.024.2274992
GCAGAGT156550.023.451439
ACATGGG86600.022.8988133
GAGTACT102850.022.21179812-13
TAAGGTG16850.021.7290555
AGTACTT106800.021.27911812-13
CAGAGTA146200.021.27829610-11
GTACTTT106500.021.2275714-15
Produced by FastQC (version 0.11.5)