##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937951_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2913429 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33441933886153 33.0 31.0 34.0 31.0 34.0 2 32.58519703071536 34.0 31.0 34.0 31.0 34.0 3 32.655824116530724 34.0 31.0 34.0 31.0 34.0 4 36.15693020149109 37.0 35.0 37.0 35.0 37.0 5 36.07032023090317 37.0 35.0 37.0 35.0 37.0 6 36.12308451656107 37.0 36.0 37.0 35.0 37.0 7 36.089512049203876 37.0 35.0 37.0 35.0 37.0 8 36.07980081203283 37.0 35.0 37.0 35.0 37.0 9 37.74260982505494 39.0 38.0 39.0 35.0 39.0 10-11 37.70065256438375 39.0 37.0 39.0 35.0 39.0 12-13 37.60879379590167 39.0 37.0 39.0 35.0 39.0 14-15 38.79584898070281 40.0 38.0 41.0 35.0 41.0 16-17 38.68169775203034 40.0 38.0 41.0 34.5 41.0 18-19 38.94608844080292 40.0 38.0 41.0 35.0 41.0 20-21 38.90671061488027 40.0 38.0 41.0 35.0 41.0 22-23 38.78464963450285 40.0 38.0 41.0 34.5 41.0 24-25 38.65948252042524 40.0 38.0 41.0 34.0 41.0 26-27 38.46714730305767 40.0 38.0 41.0 34.0 41.0 28-29 38.32769650470288 40.0 38.0 41.0 34.0 41.0 30-31 38.06786521998649 40.0 37.5 41.0 33.5 41.0 32-33 37.823298765818556 40.0 37.0 41.0 33.0 41.0 34-35 37.71718360049275 40.0 37.0 41.0 33.0 41.0 36-37 37.64940367518824 40.0 37.0 41.0 33.0 41.0 38-39 37.43374250754008 40.0 37.0 41.0 32.0 41.0 40-41 37.17877542236313 39.5 36.0 41.0 31.5 41.0 42-43 36.88558327661323 39.0 36.0 41.0 31.0 41.0 44-45 36.58851683016816 39.0 35.0 40.5 30.0 41.0 46-47 36.195838134377055 39.0 35.0 40.0 30.0 41.0 48-49 35.74199302608713 39.0 35.0 40.0 28.5 41.0 50-51 35.31243236063072 38.0 34.5 40.0 27.0 41.0 52-53 34.70313829511548 38.0 34.0 40.0 26.0 41.0 54-55 34.241507344095226 38.0 33.0 40.0 24.0 41.0 56-57 33.79380396776445 37.0 33.0 40.0 23.0 41.0 58-59 33.20033077861173 36.5 32.0 39.0 21.5 40.5 60-61 32.639833680518734 36.0 31.0 39.0 19.5 40.0 62-63 31.99032034073938 35.5 31.0 39.0 16.5 40.0 64-65 31.334461728773896 35.0 30.0 38.0 10.5 40.0 66-67 30.682779981938808 34.5 29.0 38.0 7.5 40.0 68-69 29.956682314894238 34.0 28.0 37.0 4.5 39.5 70-71 29.252446515772306 34.0 27.5 36.5 2.0 39.0 72-73 28.501130454869504 33.5 26.0 36.0 2.0 39.0 74-75 27.772686411784875 33.0 25.5 35.0 2.0 38.0 76-77 26.565924894685953 31.5 24.5 34.0 2.0 36.0 78-79 27.188165045381233 32.5 25.5 35.0 2.0 37.0 80-81 26.863524218369488 32.5 25.0 35.0 2.0 36.5 82-83 26.226983221489178 32.0 23.5 35.0 2.0 36.0 84-85 25.48174402053388 31.5 20.5 34.5 2.0 35.5 86-87 24.65075740647876 31.0 17.5 34.0 2.0 35.0 88-89 23.720275833047587 30.5 8.5 34.0 2.0 35.0 90-91 22.93901945096311 30.0 2.0 34.0 2.0 35.0 92-93 21.874262767343907 29.0 2.0 34.0 2.0 35.0 94-95 20.832541139667384 29.0 2.0 34.0 2.0 35.0 96-97 19.652304895708802 27.0 2.0 33.5 2.0 35.0 98-99 18.13189235090335 24.5 2.0 33.0 2.0 34.5 100-101 14.618473455162285 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2717701827764216 1101 2 0.31841447344642404 1101 3 0.2765071666206538 1101 4 0.10829441940546758 1101 5 0.09530902057564816 1101 6 0.16863710051302405 1101 7 0.2611222637725419 1101 8 0.22883050380033865 1101 9 0.12951071036896877 1101 10-11 0.26113196560503127 1101 12-13 0.36031761841577037 1101 14-15 0.5151429286253801 1101 16-17 0.5477714167229095 1101 18-19 0.38604729971921614 1101 20-21 0.4244306427126645 1101 22-23 0.4920095104199831 1101 24-25 0.488268259434939 1101 26-27 0.5607342165105038 1101 28-29 0.5686659854601501 1101 30-31 0.6512726778687608 1101 32-33 0.6656995027719077 1101 34-35 0.6651290933655147 1101 36-37 0.7058986048396392 1101 38-39 0.7483633709198614 1101 40-41 0.9545311020277296 1101 42-43 1.056960627347955 1101 44-45 0.9607848855616652 1101 46-47 0.9669423684606926 1101 48-49 1.2537945591922153 1101 50-51 1.2425637035229258 1101 52-53 1.2838719598185335 1101 54-55 1.5440852312546625 1101 56-57 1.537625998897063 1101 58-59 1.9163412204697536 1101 60-61 1.7586699596861521 1101 62-63 1.842719948929492 1101 64-65 2.1058888903872877 1101 66-67 2.3449727731555825 1101 68-69 2.53245933369384 1101 70-71 2.4984416532044875 1101 72-73 2.5618618820685803 1101 74-75 2.6840243524157366 1101 76-77 2.623619682247277 1101 78-79 2.8787886325728245 1101 80-81 3.099606899204801 1101 82-83 3.266413076241509 1101 84-85 3.3536806398997214 1101 86-87 3.6562141844220086 1101 88-89 3.9646094184668925 1101 90-91 4.06635374146418 1101 92-93 4.353446038652386 1101 94-95 4.844544947241875 1101 96-97 5.024510036311735 1101 98-99 5.191961459050592 1101 100-101 4.784507277428645 1102 1 0.2125632957234629 1102 2 0.17105988159254792 1102 3 0.1801333281053843 1102 4 0.1644118522582687 1102 5 -0.012369208122350983 1102 6 0.13859666550354177 1102 7 0.14958109755117022 1102 8 0.21034276867596446 1102 9 0.18303222905014138 1102 10-11 0.20556014223356556 1102 12-13 0.28313291294971066 1102 14-15 0.37186669066014133 1102 16-17 0.42752527378162597 1102 18-19 0.3338899447130501 1102 20-21 0.2340890782999523 1102 22-23 0.3418977570567492 1102 24-25 0.33125940314887004 1102 26-27 0.3335143221722987 1102 28-29 0.27250795523075055 1102 30-31 0.40118547553245776 1102 32-33 0.29420481273632504 1102 34-35 0.38767827380352315 1102 36-37 0.5374701617050803 1102 38-39 0.5671605553621788 1102 40-41 0.6744052843133659 1102 42-43 0.7676378325732642 1102 44-45 0.7164590562016642 1102 46-47 0.768050356026734 1102 48-49 0.8750163045154764 1102 50-51 0.8812397732982262 1102 52-53 0.9686731664861057 1102 54-55 0.9775082770824994 1102 56-57 1.230730463656684 1102 58-59 1.3249086610489869 1102 60-61 1.2160327762740124 1102 62-63 1.2860655848374876 1102 64-65 1.4334133070414445 1102 66-67 1.5249059059002121 1102 68-69 1.7880500812320221 1102 70-71 2.0603004430880105 1102 72-73 2.1095443435091923 1102 74-75 2.2595765685588347 1102 76-77 2.346269222356433 1102 78-79 2.4306426690192033 1102 80-81 2.8583208341101454 1102 82-83 2.8742181192519958 1102 84-85 3.0337564037529923 1102 86-87 3.255279817687338 1102 88-89 3.662481597478312 1102 90-91 3.6924945103900235 1102 92-93 3.82039523342349 1102 94-95 3.88514498329857 1102 96-97 4.112416290020985 1102 98-99 4.573157583963528 1102 100-101 4.05563123855908 1103 1 0.2593894648877608 1103 2 0.23829138717290732 1103 3 0.23815721919129373 1103 4 0.17082575106186226 1103 5 0.08945582352669845 1103 6 0.1334408367991884 1103 7 0.18686901082570273 1103 8 0.1894995705273388 1103 9 0.26413978792064086 1103 10-11 0.34038386546794186 1103 12-13 0.3678097660926838 1103 14-15 0.5052640296364217 1103 16-17 0.4453027594701666 1103 18-19 0.3801092576538352 1103 20-21 0.37774813830959886 1103 22-23 0.46703069394697394 1103 24-25 0.5180674373809993 1103 26-27 0.5326139162589598 1103 28-29 0.5346306566607524 1103 30-31 0.57313845859818 1103 32-33 0.5963364836645866 1103 34-35 0.7071648081265565 1103 36-37 0.5462957016982628 1103 38-39 0.5927886651601568 1103 40-41 0.7464456492326192 1103 42-43 0.7949924891470701 1103 44-45 0.9204579937950754 1103 46-47 0.998202211591682 1103 48-49 1.0165792613584728 1103 50-51 1.181493591594041 1103 52-53 1.2147171714541685 1103 54-55 1.4453372775168205 1103 56-57 1.5055531963275328 1103 58-59 1.8043069807920133 1103 60-61 1.812916147760376 1103 62-63 1.8829558957111558 1103 64-65 1.9963090562177008 1103 66-67 2.046191047773945 1103 68-69 2.3297601530859744 1103 70-71 2.216709062514596 1103 72-73 2.5567678538268375 1103 74-75 2.7078924200788066 1103 76-77 2.6846831396650614 1103 78-79 2.8395123217450475 1103 80-81 2.8993629559189813 1103 82-83 3.1340798050169454 1103 84-85 3.2746217208585016 1103 86-87 3.6169572825849237 1103 88-89 3.6100961626807937 1103 90-91 3.933526372790954 1103 92-93 4.026705939455802 1103 94-95 3.9656312629052657 1103 96-97 4.292696537857804 1103 98-99 4.5305554606742895 1103 100-101 4.0634311098946725 1104 1 0.2545620312318704 1104 2 0.20949450182042995 1104 3 0.20215786721078643 1104 4 0.15605163362494778 1104 5 0.14069194147455732 1104 6 0.16008804211128336 1104 7 0.17463816219017758 1104 8 0.22806399965747914 1104 9 0.28723482870225325 1104 10-11 0.3280138588008228 1104 12-13 0.33574915757746027 1104 14-15 0.4335257277382709 1104 16-17 0.502889260725695 1104 18-19 0.4518202991166973 1104 20-21 0.4528431084737008 1104 22-23 0.4604658618816373 1104 24-25 0.4841326090334732 1104 26-27 0.4978482936929325 1104 28-29 0.5646264819198095 1104 30-31 0.5797567730413249 1104 32-33 0.6589620114785362 1104 34-35 0.6770724030395954 1104 36-37 0.7113399830301077 1104 38-39 0.7464585947711271 1104 40-41 0.7779023742002664 1104 42-43 0.8449258179110402 1104 44-45 0.8594808136352654 1104 46-47 1.0681452191082954 1104 48-49 1.0827912239644277 1104 50-51 1.1879784597158505 1104 52-53 1.2531920305826247 1104 54-55 1.3954117374486614 1104 56-57 1.3941270757419062 1104 58-59 1.7835031146911149 1104 60-61 1.8914399614381594 1104 62-63 2.015608029709952 1104 64-65 2.097182792112374 1104 66-67 2.2144050686725762 1104 68-69 2.3777216436969617 1104 70-71 2.571716778515899 1104 72-73 2.663534516962624 1104 74-75 2.6407865748183816 1104 76-77 2.5975767024637584 1104 78-79 2.7403331464329277 1104 80-81 3.007187064321009 1104 82-83 3.2369536779926626 1104 84-85 3.4765023793460017 1104 86-87 3.544723619633981 1104 88-89 3.86934523894147 1104 90-91 4.02518686042562 1104 92-93 4.306587530509816 1104 94-95 4.3353653256759195 1104 96-97 4.507012108810745 1104 98-99 4.831028546057091 1104 100-101 4.2482529668443725 1105 1 0.193635508735305 1105 2 0.17857947699766896 1105 3 0.19601034410636942 1105 4 0.13179890971356656 1105 5 0.03225705020319225 1105 6 0.02838175241837604 1105 7 0.11894763768878391 1105 8 0.08292525685927643 1105 9 0.22428991186649938 1105 10-11 0.1394606122590858 1105 12-13 0.20054400262545613 1105 14-15 0.3390907812082702 1105 16-17 0.2705643893959149 1105 18-19 0.3320750802004042 1105 20-21 0.2413517118553301 1105 22-23 0.373672239309748 1105 24-25 0.37576194380675787 1105 26-27 0.3508260312493192 1105 28-29 0.41723158723598175 1105 30-31 0.44312652698529575 1105 32-33 0.516083177183674 1105 34-35 0.500003500369445 1105 36-37 0.47434696888486627 1105 38-39 0.5255806223170651 1105 40-41 0.5943497917316023 1105 42-43 0.5683719838827699 1105 44-45 0.5686560736659203 1105 46-47 0.6262304056255772 1105 48-49 0.6268596215512758 1105 50-51 0.6701508850754934 1105 52-53 0.7626607672829877 1105 54-55 1.06047558056067 1105 56-57 1.013560737751014 1105 58-59 1.1229898686147166 1105 60-61 1.1818057881412543 1105 62-63 1.250153546828976 1105 64-65 1.3697918140054561 1105 66-67 1.5346169806891936 1105 68-69 1.7431696555009815 1105 70-71 1.9625722500417098 1105 72-73 2.0628198386211984 1105 74-75 1.970294176758447 1105 76-77 1.9803796821644504 1105 78-79 2.3553725351754835 1105 80-81 2.5118601755663335 1105 82-83 2.48763762477763 1105 84-85 2.699383113762341 1105 86-87 2.9498423626842403 1105 88-89 3.1325804090265805 1105 90-91 3.316309221462273 1105 92-93 3.497029386308043 1105 94-95 3.77422625132591 1105 96-97 3.9201658935909656 1105 98-99 3.8896731050689475 1105 100-101 3.4087222241392396 1106 1 0.1996853398269991 1106 2 0.10933987376682808 1106 3 0.10788013262307317 1106 4 0.0940873950011536 1106 5 -0.021047632690404328 1106 6 -0.0351465712843293 1106 7 0.07704097597552817 1106 8 0.11531394914222659 1106 9 0.2509597380316464 1106 10-11 0.18265278602802937 1106 12-13 0.14935342962693454 1106 14-15 0.13878590699036408 1106 16-17 0.156574220193626 1106 18-19 0.09473223818331888 1106 20-21 0.09487274294873771 1106 22-23 0.08800489561897251 1106 24-25 0.05246262605454177 1106 26-27 -0.03349299764824565 1106 28-29 0.24929655736733736 1106 30-31 0.36053126609077424 1106 32-33 0.40024727369552693 1106 34-35 0.22929276257122666 1106 36-37 0.34721617441956454 1106 38-39 0.2423197685744185 1106 40-41 0.2254380002983325 1106 42-43 0.1917819710116433 1106 44-45 0.25341418829408013 1106 46-47 0.43848240415851336 1106 48-49 0.40282377633761257 1106 50-51 0.6286859643992173 1106 52-53 0.7456828094080024 1106 54-55 0.578154527466161 1106 56-57 0.650562633687187 1106 58-59 0.9195064504103669 1106 60-61 0.9900221951371364 1106 62-63 0.95489639818706 1106 64-65 1.1796391006972264 1106 66-67 1.4607782445581279 1106 68-69 1.465222200220964 1106 70-71 1.4147162202795158 1106 72-73 1.7901274598957997 1106 74-75 2.0514238033630967 1106 76-77 2.0643402328465434 1106 78-79 2.152449872633394 1106 80-81 1.9670955032797437 1106 82-83 2.4697613672707597 1106 84-85 2.707307010310654 1106 86-87 2.8708030193415084 1106 88-89 3.033763834035156 1106 90-91 3.1341790896381667 1106 92-93 3.082822928176178 1106 94-95 3.335457794279403 1106 96-97 3.931418169271513 1106 98-99 3.992871291210193 1106 100-101 3.420485587027869 1107 1 0.2212746786953943 1107 2 0.21929035891447768 1107 3 0.14307619969532936 1107 4 0.11070270297427243 1107 5 0.017707031819917063 1107 6 0.07609022217294381 1107 7 0.1386802094761137 1107 8 0.16323649913119453 1107 9 0.3070264558259268 1107 10-11 0.26668341726191613 1107 12-13 0.26535422969647726 1107 14-15 0.4667002440894663 1107 16-17 0.3849256537540384 1107 18-19 0.32239627022274675 1107 20-21 0.3053879064728946 1107 22-23 0.3269357297517601 1107 24-25 0.40344925189628356 1107 26-27 0.4354244935526239 1107 28-29 0.41643109672875056 1107 30-31 0.4446257967907883 1107 32-33 0.4606477015084778 1107 34-35 0.43467408625228643 1107 36-37 0.4425224692765468 1107 38-39 0.5347340338770437 1107 40-41 0.4364463350794452 1107 42-43 0.5457823006364393 1107 44-45 0.7054329834178006 1107 46-47 0.7115331419019739 1107 48-49 0.8315354823219465 1107 50-51 0.8676854603168209 1107 52-53 0.9750514501301168 1107 54-55 1.058253641566374 1107 56-57 1.0851762390409476 1107 58-59 1.2450383622257206 1107 60-61 1.3349386128269671 1107 62-63 1.4116908947310023 1107 64-65 1.4535434991386147 1107 66-67 1.8227878297191822 1107 68-69 2.091797276849082 1107 70-71 2.1048048343712296 1107 72-73 2.3290459119999554 1107 74-75 2.320832392102396 1107 76-77 2.094547318959755 1107 78-79 2.204399535768669 1107 80-81 2.3827944446826095 1107 82-83 2.6868252012700715 1107 84-85 2.706954357596654 1107 86-87 2.922046713941125 1107 88-89 3.280066858083419 1107 90-91 3.262581992410066 1107 92-93 3.58954358466724 1107 94-95 3.590293265813461 1107 96-97 3.8286704198890753 1107 98-99 4.017191428017746 1107 100-101 3.401975599601599 1108 1 0.22901579708016584 1108 2 0.21309842329667816 1108 3 0.1649221249130619 1108 4 0.15789725110280273 1108 5 0.0690902793946222 1108 6 0.17648940553303305 1108 7 0.12900829145509363 1108 8 0.2084806872349958 1108 9 0.15108725304678927 1108 10-11 0.20431562340368714 1108 12-13 0.26504713159747695 1108 14-15 0.34276234163447583 1108 16-17 0.36884783470453897 1108 18-19 0.3285661846290182 1108 20-21 0.3453232158513515 1108 22-23 0.39169689488459625 1108 24-25 0.3944990491118432 1108 26-27 0.442287602887113 1108 28-29 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2.4487064890899113 2313 84-85 2.533730667713762 2313 86-87 2.8881255756297755 2313 88-89 2.9534288997209757 2313 90-91 3.1529406308429593 2313 92-93 3.2577183268770256 2313 94-95 3.3034072078023122 2313 96-97 3.452847791484494 2313 98-99 3.3135548243248074 2313 100-101 3.27902453992764 2314 1 -0.17153512096557932 2314 2 -0.16012645587235852 2314 3 -0.19571408245857214 2314 4 -0.16055733229801916 2314 5 -0.04693374162211228 2314 6 -0.13722728709372234 2314 7 -0.13822496727199507 2314 8 -0.14400112873512683 2314 9 -0.20417091852489477 2314 10-11 -0.172583129164245 2314 12-13 -0.18719649164547292 2314 14-15 -0.20274717469474268 2314 16-17 -0.1673659339598217 2314 18-19 -0.22201935914181803 2314 20-21 -0.20058387760518315 2314 22-23 -0.1260646436908317 2314 24-25 -0.0968774438877702 2314 26-27 -0.10058835857336845 2314 28-29 -0.06264994945932756 2314 30-31 -0.18306657161058837 2314 32-33 -0.020196693550161626 2314 34-35 0.1248837852720257 2314 36-37 -0.010309158321533118 2314 38-39 0.08826395975331991 2314 40-41 0.16787697968644721 2314 42-43 0.30337238023151514 2314 44-45 0.40260854068061036 2314 46-47 0.5036796369077194 2314 48-49 0.5078128266739839 2314 50-51 0.5030282430629001 2314 52-53 0.7002888616528864 2314 54-55 0.8799815543414198 2314 56-57 1.0542594263282012 2314 58-59 1.2717950288034032 2314 60-61 1.3844216166157466 2314 62-63 1.562162516232327 2314 64-65 1.6011021408457076 2314 66-67 1.6499656535902147 2314 68-69 2.019546507228693 2314 70-71 2.1887252327071494 2314 72-73 2.3888233695760377 2314 74-75 2.5322927929889154 2314 76-77 2.5186311002876884 2314 78-79 2.505630120024964 2314 80-81 2.631406798622791 2314 82-83 2.8352027363001255 2314 84-85 3.115448320345788 2314 86-87 3.245494260148927 2314 88-89 3.4199215845572013 2314 90-91 3.7363548569933585 2314 92-93 4.007619950112556 2314 94-95 4.245249655143784 2314 96-97 4.533747781854924 2314 98-99 4.772936950136959 2314 100-101 4.748759637786982 2315 1 -0.24789602188241133 2315 2 -0.2639356485035691 2315 3 -0.2811613118832028 2315 4 -0.19683149521949872 2315 5 -0.14900397163835066 2315 6 -0.15107023318468293 2315 7 -0.1894542271151991 2315 8 -0.18903950471289477 2315 9 -0.21543703316832108 2315 10-11 -0.24346442690149672 2315 12-13 -0.20653526189438765 2315 14-15 -0.258172138061596 2315 16-17 -0.1671526188775445 2315 18-19 -0.2638604051663478 2315 20-21 -0.2427698498321007 2315 22-23 -0.2027322685626345 2315 24-25 -0.2106254880386942 2315 26-27 -0.21366650396623754 2315 28-29 -0.17273712155017762 2315 30-31 -0.06908664120042829 2315 32-33 -0.11771362120100548 2315 34-35 -0.09537415224716028 2315 36-37 -0.00653824166943906 2315 38-39 -0.03560150492655367 2315 40-41 -0.0856850227454089 2315 42-43 0.06701443042677369 2315 44-45 0.21438989258303565 2315 46-47 0.15757745967454895 2315 48-49 0.34262455779614953 2315 50-51 0.36440694143445285 2315 52-53 0.36224159833009395 2315 54-55 0.5408981299417306 2315 56-57 0.7559180305849011 2315 58-59 0.9301123769462407 2315 60-61 1.0990336859268837 2315 62-63 1.0064568654240915 2315 64-65 1.3857932146164167 2315 66-67 1.6278342022836085 2315 68-69 1.9032602025362344 2315 70-71 2.0752969538091754 2315 72-73 2.220168419555545 2315 74-75 2.595835526557412 2315 76-77 2.631253076745704 2315 78-79 2.6104506814422415 2315 80-81 2.8487446835380794 2315 82-83 3.014974586395798 2315 84-85 3.1876060063199922 2315 86-87 3.3492134539994787 2315 88-89 3.771357283999798 2315 90-91 4.010913256985543 2315 92-93 4.093129390675898 2315 94-95 4.215023889538951 2315 96-97 4.6300396108965 2315 98-99 5.067151535432441 2315 100-101 5.081260478558999 2316 1 -0.35258365825125537 2316 2 -0.4375712808074752 2316 3 -0.34990032910264546 2316 4 -0.23384919960598438 2316 5 -0.3682922418658805 2316 6 -0.36709523470596395 2316 7 -0.3185263113736312 2316 8 -0.4137830870844681 2316 9 -0.35491422908707904 2316 10-11 -0.41239115278563077 2316 12-13 -0.4278777761483994 2316 14-15 -0.5159935805432312 2316 16-17 -0.4017394151072935 2316 18-19 -0.49902901079448725 2316 20-21 -0.5194377577169575 2316 22-23 -0.4438925433277845 2316 24-25 -0.48400356291485735 2316 26-27 -0.4908337835192782 2316 28-29 -0.5909430755640628 2316 30-31 -0.43940934676579246 2316 32-33 -0.3877510140621965 2316 34-35 -0.47333216408730294 2316 36-37 -0.43836586753910467 2316 38-39 -0.5308131382115278 2316 40-41 -0.43596251968283894 2316 42-43 -0.4491056350098077 2316 44-45 -0.3431785795815827 2316 46-47 -0.43199890343704794 2316 48-49 -0.09934993377426338 2316 50-51 -0.02732831323765339 2316 52-53 0.08999029649318402 2316 54-55 0.35265254999043094 2316 56-57 0.4159902371042534 2316 58-59 0.6383334749386478 2316 60-61 0.7883803585409765 2316 62-63 0.8627422678234922 2316 64-65 1.2432592164962948 2316 66-67 1.4295866739733185 2316 68-69 1.8873769827455256 2316 70-71 2.0261078210001315 2316 72-73 1.9524846425006608 2316 74-75 2.4248022189230625 2316 76-77 2.5741597845902966 2316 78-79 2.5741090874017267 2316 80-81 2.566976569149496 2316 82-83 2.8084099192069623 2316 84-85 2.904013096485844 2316 86-87 3.130970123872469 2316 88-89 3.581453319303243 2316 90-91 3.8590402550072227 2316 92-93 3.9121379588032354 2316 94-95 4.322026528104583 2316 96-97 4.597826742162116 2316 98-99 4.755223028483943 2316 100-101 4.5566297874944865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 9.0 4 29.0 5 46.0 6 133.0 7 321.0 8 906.0 9 1689.0 10 2921.0 11 4761.0 12 7107.0 13 10823.0 14 14420.0 15 19955.0 16 25495.0 17 34211.0 18 35827.0 19 32272.0 20 32854.0 21 36183.0 22 39631.0 23 43858.0 24 49245.0 25 55306.0 26 62297.0 27 71204.0 28 84458.0 29 100093.0 30 118043.0 31 138293.0 32 163042.0 33 196336.0 34 244755.0 35 311915.0 36 392945.0 37 409210.0 38 168152.0 39 4679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13189029147441 18.536988545112994 14.645937827899703 23.685183335512896 2 16.036275904494598 19.58182057859093 39.761408138940915 24.62049537797356 3 19.669198915887954 22.94470424445793 29.428232496450825 27.957864343203294 4 13.297572002914817 16.35559137485588 35.82199287496452 34.52484374726478 5 14.057139204732893 36.77498680419626 34.21115210363104 14.956721887439794 6 33.629135976884974 35.6024121404709 16.474058574964413 14.294393307679714 7 29.689276793771192 32.01653446849056 19.74072476109766 18.553463976640582 8 26.62569995009305 35.31946281726437 18.947478257526715 19.107358975115858 9 26.165147666203637 16.62635334514759 19.66603613817258 37.54246285047619 10-11 25.492332231195615 26.126739316454938 26.669210061408737 21.71171839094071 12-13 26.20952835988109 23.229946568116127 27.34789486889847 23.212630203104315 14-15 22.770337422044165 24.910616478145936 25.45588470765629 26.863161392153607 16-17 22.596620683050798 28.18462368569819 26.37297837016107 22.84577726108994 18-19 22.638461414367743 26.441643163433877 28.636067671462044 22.283827750736332 20-21 22.857310063159254 25.718869414699995 29.07834719843868 22.34547332370207 22-23 22.779119746621028 25.606996582198427 28.945973335484297 22.66791033569625 24-25 22.698408584981156 25.805365424441124 28.943951828327837 22.552274162249883 26-27 22.21056862680485 26.242438035138594 28.82265390297618 22.724339435080378 28-29 22.388704169554156 26.54298422923641 28.73576119411182 22.332550407097614 30-31 22.386455959626954 26.04654172111282 28.661741885592544 22.90526043366768 32-33 22.210340461360136 26.014414629634015 28.7007165783 23.074528330705846 34-35 22.95706981550839 26.131851711236102 28.462880007936526 22.44819846531898 36-37 22.966856330063052 25.776178886094524 28.436998332193202 22.819966451649222 38-39 22.514371470635584 26.08464398880009 28.513008038824168 22.88797650174016 40-41 22.93665295524456 25.848782569204467 28.45534874189149 22.759215733659488 42-43 22.606312546951244 25.922887457923018 28.337552258868627 23.13324773625711 44-45 22.70589131784654 26.239436025072415 28.44449684741228 22.61017580966876 46-47 23.02654758817661 26.548017224380143 27.734523088519246 22.690912098924 48-49 22.966957492356944 26.33081156259514 27.808966684961263 22.893264260086653 50-51 22.987843534199733 26.712440907260827 27.705720647388354 22.593994911151086 52-53 23.2630175645262 26.477683169900484 27.590753026759877 22.668546238813438 54-55 22.808364301996033 26.614909784999053 27.68407948160055 22.89264643140437 56-57 22.80929495952964 26.83527328712546 27.6033889281992 22.7520428251457 58-59 22.87232673252034 26.42595717966698 27.532162273389876 23.169553814422798 60-61 22.641378938700754 26.670445718773305 27.690995730460564 22.99717961206537 62-63 22.631785432217498 26.75905264895764 27.80867493252796 22.800486986296903 64-65 22.40521392489743 26.68760762661455 27.652295628278566 23.254882820209453 66-67 22.410957540560045 26.770341805313503 27.587952677730954 23.2307479763955 68-69 22.80873254766468 26.398250566048358 27.753368530474447 23.039648355812513 70-71 22.616511334238794 26.631985883301084 27.383093941880855 23.368408840579264 72-73 22.67432431388802 26.746967274799182 27.22640257909284 23.352305832219955 74-75 22.6721502394601 27.093503908967747 26.9929866147416 23.241359236830554 76-77 22.78749542206108 26.737154054552214 27.38067411287524 23.094676410511465 78-79 22.74402430949922 26.833089119384752 27.42308118715095 22.99980538396508 80-81 22.836388212232535 26.966043769073327 27.159046717147884 23.038521301546254 82-83 22.76231802873097 27.016700922504917 27.14469693537248 23.07628411339163 84-85 22.768756498221773 26.96906631901931 27.121299338859036 23.140877843899883 86-87 22.92816157694108 26.96466748691749 26.97779676326439 23.129374172877043 88-89 22.73978531826243 27.158221463437073 26.705404525045918 23.39658869325458 90-91 22.755728766121287 27.046483158279717 26.944040794337642 23.253747281261354 92-93 22.720014800429322 26.729568491286383 27.051646015742964 23.49877069254133 94-95 22.577544391006057 26.667262757136957 27.087386405293007 23.66780644656398 96-97 22.862010958228918 26.45671231851699 26.90367735316519 23.777599370088904 98-99 22.53495794817722 26.720163765789383 26.640841427747162 24.104036858286232 100-101 23.058636404044854 26.022154650070416 26.837739996409727 24.081468949475 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 660.0 1 469.0 2 399.5 3 516.0 4 417.0 5 397.5 6 770.5 7 1754.5 8 2892.5 9 3404.5 10 2913.5 11 2286.0 12 2326.0 13 2568.0 14 2768.0 15 3007.5 16 3272.5 17 3493.0 18 3747.0 19 4179.0 20 4720.0 21 5546.0 22 6825.0 23 8365.5 24 10400.0 25 13220.5 26 16889.0 27 21161.5 28 25105.0 29 28749.5 30 33133.0 31 38776.5 32 46285.0 33 50233.0 34 51051.5 35 53967.5 36 58406.5 37 63169.5 38 67306.0 39 70741.5 40 75232.0 41 81984.0 42 86226.5 43 88839.0 44 92567.0 45 97239.0 46 100294.5 47 103201.0 48 104973.0 49 103640.0 50 103290.0 51 102517.5 52 103260.0 53 106362.0 54 108967.0 55 104268.0 56 95724.0 57 89005.5 58 83384.0 59 74995.5 60 61520.0 61 50931.0 62 42899.5 63 33444.0 64 24796.0 65 18782.0 66 14402.0 67 11164.5 68 8498.5 69 6476.0 70 4983.0 71 3645.5 72 2705.5 73 2175.5 74 1719.0 75 1265.0 76 864.5 77 506.0 78 295.0 79 178.0 80 100.0 81 60.0 82 42.0 83 20.5 84 8.0 85 7.0 86 4.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014450326402325233 3 0.002642933807551171 4 0.0013729526272993095 5 0.11714718292431359 6 0.0 7 0.0 8 2.4026670977737914E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.7161907841241368E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.2310480193613779E-4 24-25 5.320191430784824E-4 26-27 0.005663429587609652 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010829163847823304 36-37 7.551239450146203E-4 38-39 0.006590172611036685 40-41 0.009816611285190064 42-43 0.005749239126815859 44-45 0.0037069720937081357 46-47 1.7161907841241368E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.7161907841241368E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.14857235237241E-5 68-69 0.0010125525626332407 70-71 0.0 72-73 5.14857235237241E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.864763136496547E-5 82-83 0.001956457493901516 84-85 0.004530743670087722 86-87 0.0031749529506296535 88-89 0.0 90-91 0.0028660386094873087 92-93 0.0 94-95 3.4323815682482736E-5 96-97 6.864763136496547E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2913429.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.46635354477585 #Duplication Level Percentage of deduplicated Percentage of total 1 72.36506404202377 35.0727077815107 2 14.669310270640082 14.219359556697073 3 5.261018634851536 7.649471674871054 4 2.5405910628764867 4.9253273866427865 5 1.3551786532676948 3.2840283862802653 6 0.8235644895183472 2.394910062955143 7 0.5386105236648892 1.8273141644015563 8 0.4080233883356656 1.582032463489099 9 0.28956224624602517 1.2630623579801368 >10 1.560299623649273 13.91700730155522 >50 0.12009631463021457 4.042091111283032 >100 0.06178401864672987 5.417975440365159 >500 0.004052718598132454 1.3540953067693458 >1k 0.0026307120723845887 2.2161745099053505 >5k 1.422006525613291E-4 0.4839525254557585 >10k+ 7.110032628066455E-5 0.3504899698384022 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10171 0.34910752930653194 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8700 0.2986171964375998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5344 0.18342647100718776 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3493 0.1198930881789122 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3375 0.11584287792837925 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3113 0.10685003821956876 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3065 0.10520249506680958 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.4323815682482736E-5 6 0.0 0.0 0.0 0.0 3.4323815682482736E-5 7 0.0 0.0 0.0 0.0 3.4323815682482736E-5 8 0.0 0.0 0.0 0.0 6.864763136496547E-5 9 0.0 0.0 0.0 0.0 6.864763136496547E-5 10-11 0.0 0.0 0.0 0.0 6.864763136496547E-5 12-13 1.7161907841241368E-5 0.0 0.0 0.0 3.089143411423446E-4 14-15 3.4323815682482736E-5 0.0 0.0 0.0 3.7756197250731014E-4 16-17 3.4323815682482736E-5 0.0 0.0 0.0 4.1188578818979286E-4 18-19 5.14857235237241E-5 0.0 0.0 0.0 4.4620960387227557E-4 20-21 6.864763136496547E-5 0.0 0.0 0.0 5.320191430784824E-4 22-23 6.864763136496547E-5 0.0 0.0 3.4323815682482736E-5 5.663429587609652E-4 24-25 1.0297144704744821E-4 0.0 0.0 1.2013335488868959E-4 5.835048666022065E-4 26-27 1.0297144704744821E-4 0.0 0.0 4.6337151171351696E-4 6.178286822846893E-4 28-29 1.7161907841241369E-4 0.0 0.0 0.0022996956507263435 6.864763136496547E-4 30-31 1.7161907841241369E-4 0.0 0.0 0.009318915957794063 6.864763136496547E-4 32-33 1.7161907841241369E-4 0.0 0.0 0.026738252416654052 6.864763136496547E-4 34-35 1.7161907841241369E-4 0.0 0.0 0.052687057072610996 6.864763136496547E-4 36-37 1.7161907841241369E-4 0.0 0.0 0.09306902622305194 6.864763136496547E-4 38-39 2.0594289409489643E-4 0.0 0.0 0.153650560902634 7.036382214908961E-4 40-41 2.2310480193613779E-4 0.0 0.0 0.21744137234852814 7.551239450146203E-4 42-43 3.2607624898358604E-4 0.0 0.0 0.28380646997060854 7.551239450146203E-4 44-45 3.7756197250731014E-4 0.0 0.0 0.36192747446393925 7.722858528558616E-4 46-47 4.2904769603103424E-4 0.0 0.0 0.45618067232803683 8.066096685383443E-4 48-49 4.8053341955475834E-4 0.0 0.0 0.554810156691651 8.580953920620685E-4 50-51 4.8053341955475834E-4 0.0 0.0 0.6628272046444241 8.924192077445511E-4 52-53 4.8053341955475834E-4 0.0 0.0 0.76300126071375 0.001115524009680689 54-55 5.148572352372411E-4 0.0 0.0 0.8712414134684593 0.001167009733204413 56-57 5.148572352372411E-4 1.7161907841241368E-5 0.0 0.9948071499253972 0.0011841716410456544 58-59 5.148572352372411E-4 3.4323815682482736E-5 0.0 1.1261129068187348 0.0012013335488868957 60-61 5.320191430784824E-4 3.4323815682482736E-5 0.0 1.2670293321031678 0.001218495456728137 62-63 5.491810509197238E-4 3.4323815682482736E-5 0.0 1.4053199854878908 0.0012699811802518613 64-65 5.491810509197238E-4 3.4323815682482736E-5 0.0 1.546339382219371 0.0012699811802518613 66-67 6.178286822846893E-4 3.4323815682482736E-5 0.0 1.710081144932655 0.0012699811802518613 68-69 6.178286822846893E-4 3.4323815682482736E-5 0.0 1.8939195017280326 0.0012699811802518613 70-71 6.178286822846893E-4 3.4323815682482736E-5 0.0 2.0842622215952407 0.0013043049959343442 72-73 6.349905901259306E-4 3.4323815682482736E-5 0.0 2.278929742238441 0.0014244383508230335 74-75 6.52152497967172E-4 3.4323815682482736E-5 0.0 2.4749015678775765 0.0014930859821879992 76-77 6.52152497967172E-4 3.4323815682482736E-5 0.0 2.6824233574938674 0.0015102478900292406 78-79 6.52152497967172E-4 5.14857235237241E-5 0.0 2.9189316094540145 0.0015102478900292406 80-81 6.864763136496547E-4 6.864763136496547E-5 0.0 3.1576022618021584 0.001527409797870482 82-83 6.864763136496547E-4 6.864763136496547E-5 0.0 3.4055575062924133 0.0016132193370766888 84-85 6.864763136496547E-4 6.864763136496547E-5 0.0 3.661372904573957 0.001681866968441654 86-87 6.864763136496547E-4 8.580953920620684E-5 0.0 3.923692665927332 0.0017848384154891023 88-89 6.864763136496547E-4 1.0297144704744821E-4 0.0 4.201526791969188 0.0018878098625365505 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5045 0.0 53.734512 1 GTATCAA 8865 0.0 42.328987 1 ATCAACG 11175 0.0 33.320312 3 TCAACGC 11385 0.0 32.661694 4 TATCAAC 11525 0.0 32.435547 2 CAACGCA 11675 0.0 31.8493 5 AACGCAG 12085 0.0 30.693563 6 TGGTATC 2200 0.0 28.752035 2 GTGGTAT 2455 0.0 27.315376 1 CGCAGAG 13710 0.0 26.882349 8 ACGCAGA 13775 0.0 26.789976 7 GTACATG 8685 0.0 25.080446 1 TACATGG 8500 0.0 24.227499 2 GCAGAGT 15655 0.0 23.45143 9 ACATGGG 8660 0.0 22.898813 3 GAGTACT 10285 0.0 22.211798 12-13 TAAGGTG 1685 0.0 21.729055 5 AGTACTT 10680 0.0 21.279118 12-13 CAGAGTA 14620 0.0 21.278296 10-11 GTACTTT 10650 0.0 21.22757 14-15 >>END_MODULE