FastQCFastQC Report
Thu 26 May 2016
SRR937950_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937950_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903231
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105190.3623204629600607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83990.28929837136624675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52460.18069523231186221No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35700.12296644669335646No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35540.12241533656812015No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31080.1070531418271574No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30990.10674314238171197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48050.057.0003471
GTATCAA86100.044.224071
ATCAACG106600.035.223563
TATCAAC108950.034.6383062
TCAACGC109400.034.3654864
CAACGCA111050.033.812085
AACGCAG115150.032.6850936
ACGCAGA131250.028.4110077
CGCAGAG131600.028.1910638
TGGTATC19100.026.8753242
TAAGGTG15400.026.8510885
GTAAGGT14600.025.7180234
GCAGAGT146650.024.6808019
GTACATG84550.024.436181
GTGGTAT22150.024.3424191
TACATGG84200.023.6518572
GAGTACT99200.022.67459912-13
ACATGGG84300.022.6653183
GTAGCCC13100.022.4948833
CAGAGTA140050.021.97806710-11
Produced by FastQC (version 0.11.5)