FastQCFastQC Report
Thu 26 May 2016
SRR937950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903231
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102490.35302047959669763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87310.30073390646490067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.18927188363585262No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34290.1181097887147113No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34100.11745534544099316No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30060.10353981477877579No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29440.10140426304348502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47500.056.903871
GTATCAA85150.042.566021
ATCAACG108450.033.111433
TATCAAC110550.032.6571272
TCAACGC110950.032.577724
CAACGCA113750.031.7340475
AACGCAG117800.030.7236616
GTGGTAT22050.027.1447051
CGCAGAG133400.027.059588
ACGCAGA133550.026.9936247
TGGTATC20750.026.3270912
TAAGGTG15750.023.8237365
GCAGAGT151650.023.5525919
GTACATG87250.023.1935541
TACATGG85400.023.02852
GAGTACT99500.022.81750112-13
ACATGGG84900.022.0991293
GTAAGGT14850.022.0691664
CAGAGTA143200.021.42657910-11
GTACTTT105450.021.28229914-15
Produced by FastQC (version 0.11.5)