##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937950_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2903231 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3443535839897 33.0 31.0 34.0 31.0 34.0 2 32.60007729319506 34.0 31.0 34.0 31.0 34.0 3 32.57390989556119 34.0 31.0 34.0 31.0 34.0 4 36.139087451188004 37.0 35.0 37.0 35.0 37.0 5 36.088919207600085 37.0 35.0 37.0 35.0 37.0 6 36.14778810229017 37.0 36.0 37.0 35.0 37.0 7 36.10271142737178 37.0 36.0 37.0 35.0 37.0 8 36.09530622950774 37.0 36.0 37.0 35.0 37.0 9 37.775563845935785 39.0 38.0 39.0 35.0 39.0 10-11 37.72840363030017 39.0 37.5 39.0 35.0 39.0 12-13 37.66185191602046 39.0 37.0 39.0 35.0 39.0 14-15 38.89011122435659 40.0 38.0 41.0 35.0 41.0 16-17 38.80465574389362 40.0 38.0 41.0 35.0 41.0 18-19 38.7820245788227 40.0 38.0 41.0 35.0 41.0 20-21 38.775281057552775 40.0 38.0 41.0 34.5 41.0 22-23 38.80523492619085 40.0 38.0 41.0 35.0 41.0 24-25 38.74290712657725 40.0 38.0 41.0 34.5 41.0 26-27 38.55517576796335 40.0 38.0 41.0 34.0 41.0 28-29 38.467603507953726 40.0 38.0 41.0 34.0 41.0 30-31 38.241041963247156 40.0 38.0 41.0 34.0 41.0 32-33 38.029358497480914 40.0 37.0 41.0 33.5 41.0 34-35 37.801798065672344 40.0 37.0 41.0 33.0 41.0 36-37 37.72054066658836 40.0 37.0 41.0 33.0 41.0 38-39 37.664062556510316 40.0 37.0 41.0 33.0 41.0 40-41 37.52564039857663 40.0 37.0 41.0 33.0 41.0 42-43 37.32190239081906 40.0 37.0 41.0 32.0 41.0 44-45 37.058092862744985 40.0 36.0 41.0 31.0 41.0 46-47 36.690672047797776 39.0 36.0 41.0 30.5 41.0 48-49 36.2928113539708 39.0 35.0 40.5 30.0 41.0 50-51 35.966663348524456 39.0 35.0 40.0 29.0 41.0 52-53 35.44510168154032 38.0 35.0 40.0 28.0 41.0 54-55 35.083254484400314 38.0 34.0 40.0 27.0 41.0 56-57 34.76762079903391 38.0 34.0 40.0 26.0 41.0 58-59 34.34113992307192 37.5 33.5 40.0 25.5 41.0 60-61 33.87190220137495 37.0 33.0 39.5 24.5 41.0 62-63 33.38254499900283 36.0 32.5 39.0 23.0 40.5 64-65 32.893951428598 36.0 32.0 39.0 22.0 40.0 66-67 32.32774071370828 35.0 31.0 38.5 20.5 40.0 68-69 31.739640421309915 35.0 31.0 38.0 18.5 40.0 70-71 31.086237195731236 34.5 30.0 37.0 16.0 39.0 72-73 30.57439383913991 34.0 29.0 36.5 12.5 39.0 74-75 29.97436201253018 34.0 29.0 36.0 8.0 39.0 76-77 28.862394862826967 32.5 28.0 35.0 8.0 37.0 78-79 29.57461824429403 34.0 29.0 35.0 7.0 37.0 80-81 29.35213835895249 34.0 29.0 35.0 4.0 37.0 82-83 28.82614800544635 34.0 28.5 35.0 2.0 36.5 84-85 28.244608162423177 33.0 27.0 35.0 2.0 36.0 86-87 27.60863723899338 33.0 26.0 35.0 2.0 35.5 88-89 26.866141378347088 32.0 25.0 34.5 2.0 35.0 90-91 26.217062817254295 32.0 24.5 34.0 2.0 35.0 92-93 25.447746837919546 31.5 21.5 34.0 2.0 35.0 94-95 24.542246552203387 31.0 18.0 34.0 2.0 35.0 96-97 23.53493435417299 31.0 4.5 34.0 2.0 35.0 98-99 22.302940930294557 30.0 2.0 34.0 2.0 35.0 100-101 18.84959429683687 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12689504947685037 1101 2 0.04044933380606608 1101 3 0.1802298094563568 1101 4 0.10503661932086317 1101 5 0.10205650883544592 1101 6 0.08403441592137995 1101 7 0.14296666660921176 1101 8 0.18320277750354563 1101 9 0.15980063756332896 1101 10-11 0.20648797375780958 1101 12-13 0.15317083851074642 1101 14-15 0.32408317359848837 1101 16-17 0.31325328408423303 1101 18-19 0.27158432216923956 1101 20-21 0.27846954817312763 1101 22-23 0.34303877417741546 1101 24-25 0.2874024197807259 1101 26-27 0.43732623263979065 1101 28-29 0.34032636274960026 1101 30-31 0.44538160422588646 1101 32-33 0.4240738211458961 1101 34-35 0.39170985278075676 1101 36-37 0.39303699447447116 1101 38-39 0.6060344922390044 1101 40-41 0.6273594383901155 1101 42-43 0.616027877063793 1101 44-45 0.659746918147313 1101 46-47 0.5828965580941414 1101 48-49 0.7006137615445098 1101 50-51 0.6438650403114252 1101 52-53 0.6424973321178697 1101 54-55 0.7204015935447998 1101 56-57 0.8123676854400514 1101 58-59 0.9180210390462733 1101 60-61 0.8892555788842813 1101 62-63 1.1571257467696157 1101 64-65 1.0399857584393786 1101 66-67 0.98875277295069 1101 68-69 1.0708392753605587 1101 70-71 1.1362732806488225 1101 72-73 1.1324074775414417 1101 74-75 1.3251004806727344 1101 76-77 1.299997374977032 1101 78-79 1.4243483769060994 1101 80-81 1.5903789005272877 1101 82-83 1.6897796359826032 1101 84-85 1.696310097840449 1101 86-87 1.8582862155685511 1101 88-89 2.296328997743565 1101 90-91 2.4386627925887154 1101 92-93 2.6377234602303616 1101 94-95 2.751661362906205 1101 96-97 2.9991911518675387 1101 98-99 3.3260131526563974 1101 100-101 3.728877913869699 1102 1 0.09395902074830786 1102 2 0.07876441777241183 1102 3 0.19527451110320726 1102 4 0.09442097021870666 1102 5 0.029668177085454772 1102 6 0.06533229497727433 1102 7 0.09926667282807244 1102 8 0.040987946793194396 1102 9 0.03532101188156389 1102 10-11 0.11501157433827558 1102 12-13 0.15529969280645872 1102 14-15 0.2804672255226066 1102 16-17 0.19868506694343324 1102 18-19 0.2839569948773075 1102 20-21 0.27310610514063427 1102 22-23 0.2868432266222456 1102 24-25 0.2594656143449754 1102 26-27 0.28545219555507373 1102 28-29 0.15264000831142965 1102 30-31 0.1036409031806187 1102 32-33 0.13083959148983837 1102 34-35 0.11102907216008617 1102 36-37 0.16561250355596258 1102 38-39 0.21856752831594406 1102 40-41 0.17489892881293656 1102 42-43 0.13196624736293217 1102 44-45 0.16940911686102567 1102 46-47 0.16914273863316254 1102 48-49 0.31298612200847487 1102 50-51 0.38505630882131925 1102 52-53 0.34287862120204693 1102 54-55 0.34299500462171295 1102 56-57 0.3650050162525389 1102 58-59 0.37526883622916074 1102 60-61 0.5254638087542318 1102 62-63 0.4450946300832115 1102 64-65 0.547546879572657 1102 66-67 0.5861163398400109 1102 68-69 0.7533854413021679 1102 70-71 0.9276132944448037 1102 72-73 0.8791263123921524 1102 74-75 0.9199224455884654 1102 76-77 0.8536857324611447 1102 78-79 0.9238450133865683 1102 80-81 1.1567630383185303 1102 82-83 1.2721295304831273 1102 84-85 1.280419292726826 1102 86-87 1.416158774177422 1102 88-89 1.4711140111713625 1102 90-91 1.7291198060727169 1102 92-93 2.0816935321531886 1102 94-95 2.071989143758721 1102 96-97 2.252554866552405 1102 98-99 2.600909741311476 1102 100-101 2.509638316737451 1103 1 0.29534008300430514 1103 2 0.278358469362189 1103 3 0.3240604861155205 1103 4 0.17583244211643745 1103 5 0.13884970589338508 1103 6 0.21171042541889307 1103 7 0.2550028120727603 1103 8 0.2555202906709084 1103 9 0.24474267024777419 1103 10-11 0.3597238376989651 1103 12-13 0.3414515024085958 1103 14-15 0.542850333702745 1103 16-17 0.4876799432487928 1103 18-19 0.5715567209874663 1103 20-21 0.5394923773957672 1103 22-23 0.5722943833562866 1103 24-25 0.5381388541140879 1103 26-27 0.5163903253874977 1103 28-29 0.46420128830857976 1103 30-31 0.5954583171426577 1103 32-33 0.7355299550855889 1103 34-35 0.7224551523623362 1103 36-37 0.6765898321273127 1103 38-39 0.7945749746167792 1103 40-41 0.8063700206155318 1103 42-43 0.8969970314138536 1103 44-45 0.8771299104054506 1103 46-47 0.8300044762979653 1103 48-49 1.014610074297316 1103 50-51 1.111442352623392 1103 52-53 1.0676340812303806 1103 54-55 1.1572896303902027 1103 56-57 1.3125943921695153 1103 58-59 1.2726575542013094 1103 60-61 1.4054958048756063 1103 62-63 1.4292001665164022 1103 64-65 1.4808230361797712 1103 66-67 1.4021080228791476 1103 68-69 1.454824886008911 1103 70-71 1.661462138472281 1103 72-73 1.5518620569207044 1103 74-75 1.6846675046562751 1103 76-77 1.3636147152865483 1103 78-79 1.8586029419772139 1103 80-81 1.9821068601860574 1103 82-83 2.1095607407259465 1103 84-85 2.2776277231527153 1103 86-87 2.2461306744500433 1103 88-89 2.461800741094148 1103 90-91 2.6311029690815957 1103 92-93 2.884430653746062 1103 94-95 2.9003456904621423 1103 96-97 3.1341324111388253 1103 98-99 3.440748751703552 1103 100-101 3.4168927169973813 1104 1 0.12032299660258161 1104 2 0.0914793051108802 1104 3 0.12029306872067025 1104 4 0.010507192171900215 1104 5 -0.031683318274481564 1104 6 0.015437765808648862 1104 7 0.08198599762213377 1104 8 0.06488748549524814 1104 9 0.07684685614782438 1104 10-11 0.09345999860814658 1104 12-13 0.025337862629299934 1104 14-15 0.14129797595821714 1104 16-17 0.0922878595307921 1104 18-19 0.10066913161931978 1104 20-21 0.04254176335880544 1104 22-23 0.052358309757728705 1104 24-25 0.06486229951990197 1104 26-27 0.07010961459318565 1104 28-29 0.1384291350283533 1104 30-31 0.1971293403944756 1104 32-33 0.19188873552801056 1104 34-35 0.23358278331037496 1104 36-37 0.2641824654728211 1104 38-39 0.23756431663824173 1104 40-41 0.19610391109080183 1104 42-43 0.26550934256886194 1104 44-45 0.39327231080336134 1104 46-47 0.36720139394687834 1104 48-49 0.31806210892099074 1104 50-51 0.5424345358182379 1104 52-53 0.5072772616985617 1104 54-55 0.5462588820236931 1104 56-57 0.6423784951960485 1104 58-59 0.5424093451424667 1104 60-61 0.6174105732519877 1104 62-63 0.5718691608698379 1104 64-65 0.5014640356716527 1104 66-67 0.548057874737367 1104 68-69 0.7347802108831338 1104 70-71 0.8311307785736162 1104 72-73 0.7631132735440715 1104 74-75 0.84375308695369 1104 76-77 0.8125731157130822 1104 78-79 0.927006249045796 1104 80-81 1.0825444867685619 1104 82-83 1.2009734372642953 1104 84-85 1.5577275220520264 1104 86-87 1.4949346263290089 1104 88-89 1.606031441511373 1104 90-91 1.5798379491504413 1104 92-93 1.86995641395675 1104 94-95 2.1689524241146607 1104 96-97 2.5687818446933015 1104 98-99 2.8541544651815904 1104 100-101 2.915015896535209 1105 1 0.1253049431102795 1105 2 0.12181765594282723 1105 3 0.1794868820783151 1105 4 0.08136121024101328 1105 5 0.08793334806546227 1105 6 0.08956645402121666 1105 7 0.1377598312943249 1105 8 0.09326858767919788 1105 9 0.026662234393988626 1105 10-11 0.024256218477866298 1105 12-13 0.04699302691602725 1105 14-15 0.024741670338144672 1105 16-17 0.11181421687641802 1105 18-19 0.20128701836005547 1105 20-21 0.1876375497032683 1105 22-23 0.23614807786163539 1105 24-25 0.2689068555012355 1105 26-27 0.20527645087994273 1105 28-29 0.2305396021774868 1105 30-31 0.24133594752631637 1105 32-33 0.16131619488923832 1105 34-35 0.18465101333936929 1105 36-37 0.22151849855111294 1105 38-39 0.23142482235753192 1105 40-41 0.2205285401278445 1105 42-43 0.2940615600290215 1105 44-45 0.32146486499622995 1105 46-47 0.387093960134429 1105 48-49 0.4039850144561612 1105 50-51 0.38089196870588893 1105 52-53 0.4015847271645612 1105 54-55 0.40733969262500125 1105 56-57 0.40193855062189243 1105 58-59 0.4209913721020442 1105 60-61 0.5212272856435334 1105 62-63 0.6768616179534064 1105 64-65 0.7902784349612304 1105 66-67 0.7883191173709605 1105 68-69 0.7906646880251778 1105 70-71 1.0298160484057917 1105 72-73 0.8259206095937657 1105 74-75 0.7975274856939265 1105 76-77 0.8510269823147176 1105 78-79 1.1824310186299414 1105 80-81 1.2932604341917582 1105 82-83 1.302034562409812 1105 84-85 1.48292871540664 1105 86-87 1.5941373509715255 1105 88-89 1.7703464083609752 1105 90-91 1.6159227275218235 1105 92-93 2.0234267702010342 1105 94-95 2.1016725002338212 1105 96-97 2.175680271219111 1105 98-99 2.6705312714307006 1105 100-101 2.8200300282204296 1106 1 0.06373751173283893 1106 2 0.05049071882864098 1106 3 0.07785704905528945 1106 4 0.06901901540094002 1106 5 0.025106590731361678 1106 6 -0.024003827011185308 1106 7 0.03271049886039634 1106 8 -0.036948442146119476 1106 9 -0.02547615343514309 1106 10-11 0.024475913634880442 1106 12-13 -0.0315366337690719 1106 14-15 -0.09188454761243037 1106 16-17 0.05728000522397991 1106 18-19 -0.07942422280616057 1106 20-21 -0.017306662221749036 1106 22-23 0.05172199819677559 1106 24-25 -0.04554949687985044 1106 26-27 -0.05997662366237222 1106 28-29 -0.10109429374563916 1106 30-31 -0.05038812184672281 1106 32-33 -0.0024370140013729724 1106 34-35 -0.15406510373965432 1106 36-37 -0.05895579419489394 1106 38-39 -0.022821589720919633 1106 40-41 0.049568767598323404 1106 42-43 0.012525136930115366 1106 44-45 0.14264030872420363 1106 46-47 -0.11808023400296719 1106 48-49 0.08545954214942952 1106 50-51 0.12082163380436128 1106 52-53 0.08813220100558539 1106 54-55 0.17075467265448196 1106 56-57 0.3356283107723286 1106 58-59 0.391764668766875 1106 60-61 0.41513399557280906 1106 62-63 0.26423636142605034 1106 64-65 0.40348277374046404 1106 66-67 0.501212702705061 1106 68-69 0.02183672653229607 1106 70-71 0.13314605800704626 1106 72-73 0.28422271758556406 1106 74-75 0.44995335940133785 1106 76-77 0.5831242608639435 1106 78-79 0.705961657180243 1106 80-81 0.8436552148146248 1106 82-83 0.8626670724469925 1106 84-85 1.0326929135982823 1106 86-87 1.152489888250475 1106 88-89 1.378979984530833 1106 90-91 1.29982169024915 1106 92-93 1.2551832562381193 1106 94-95 1.742499421555049 1106 96-97 1.8554327718064805 1106 98-99 2.188089468472267 1106 100-101 2.453580399311619 1107 1 0.015062592789000462 1107 2 -0.0064231572122182 1107 3 0.1283882256020874 1107 4 0.0469475109952171 1107 5 0.039127582047797205 1107 6 0.006587197333423944 1107 7 0.0020361889945093026 1107 8 0.03591923819227105 1107 9 0.03275767192774026 1107 10-11 0.03409623261001826 1107 12-13 -0.046218651331386695 1107 14-15 0.007804225331049963 1107 16-17 0.04449273327067971 1107 18-19 0.03265264047602301 1107 20-21 -0.00990981428429194 1107 22-23 -0.006132415346961295 1107 24-25 0.08995373274759544 1107 26-27 0.02570167381332311 1107 28-29 -0.03676611345856173 1107 30-31 -0.04102649274828707 1107 32-33 -0.04745620332198541 1107 34-35 -0.0032953856821791305 1107 36-37 0.012311163662623414 1107 38-39 0.14664025962146354 1107 40-41 0.1692694108317525 1107 42-43 0.04425692053582253 1107 44-45 0.00814176327637739 1107 46-47 0.13378906325231554 1107 48-49 0.24208343314803216 1107 50-51 0.19107549480334995 1107 52-53 0.12503883372990288 1107 54-55 0.2615175932673992 1107 56-57 0.40377647823848406 1107 58-59 0.3344242699049431 1107 60-61 0.37441578834832967 1107 62-63 0.4321103176229073 1107 64-65 0.3168551054490294 1107 66-67 0.4511452750835403 1107 68-69 0.6074439368711388 1107 70-71 0.7822250379192539 1107 72-73 0.630691537673691 1107 74-75 0.7246876870011505 1107 76-77 0.9350245167776379 1107 78-79 0.9384247970275688 1107 80-81 1.0587373393862123 1107 82-83 1.2477399935478424 1107 84-85 1.4243935534666399 1107 86-87 1.5734626547262849 1107 88-89 1.7192312039486417 1107 90-91 1.8006261526705565 1107 92-93 1.9247715060812212 1107 94-95 1.8975345781402417 1107 96-97 1.778890223000328 1107 98-99 1.8013115076719863 1107 100-101 2.0783242332386074 1108 1 0.002222433305050231 1108 2 0.04873170753437961 1108 3 0.11866278434695943 1108 4 0.06756980633225851 1108 5 -0.00791167304547713 1108 6 0.0031706103547080033 1108 7 0.013992622648217434 1108 8 0.04476865228325977 1108 9 0.016118018124601008 1108 10-11 -0.01662278228487679 1108 12-13 -0.04134093856455934 1108 14-15 -0.03313606543542136 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-0.7782774704227435 2316 52-53 -0.679884088139751 2316 54-55 -0.7306834754310216 2316 56-57 -0.8732971358130612 2316 58-59 -0.9944694909500171 2316 60-61 -0.9897944222854065 2316 62-63 -1.2318017381953439 2316 64-65 -1.2727373265054354 2316 66-67 -1.1527446033409205 2316 68-69 -1.2666373533001263 2316 70-71 -1.4528074001837155 2316 72-73 -1.3724322342105388 2316 74-75 -1.2990535735685675 2316 76-77 -1.2760370823400216 2316 78-79 -1.7085557020786801 2316 80-81 -1.8376477216551024 2316 82-83 -2.1046908041093886 2316 84-85 -1.902987446076974 2316 86-87 -2.0357513036413835 2316 88-89 -2.0113138575997667 2316 90-91 -2.2547789036801476 2316 92-93 -2.852964894319385 2316 94-95 -3.0027279613179267 2316 96-97 -3.051004254123445 2316 98-99 -3.5788634156302024 2316 100-101 -4.141478578908805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 16.0 5 51.0 6 122.0 7 350.0 8 752.0 9 1400.0 10 2465.0 11 3993.0 12 5953.0 13 8755.0 14 11963.0 15 16029.0 16 20482.0 17 27791.0 18 27965.0 19 22963.0 20 21800.0 21 22251.0 22 23794.0 23 26536.0 24 30388.0 25 35195.0 26 41432.0 27 49680.0 28 60055.0 29 73030.0 30 89027.0 31 108337.0 32 136006.0 33 175963.0 34 237861.0 35 336498.0 36 470563.0 37 541940.0 38 260727.0 39 11085.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1316350645195 18.63444555393629 14.651090457493737 23.58282892405048 2 16.069972548775098 19.600360596589628 39.74116825992166 24.588498594713613 3 19.891914570555212 23.01195215829841 29.336868436698477 27.7592648344479 4 13.293671774653825 16.38846512730127 35.84558032068409 34.47228277736081 5 14.106647533933792 36.77080710533486 34.191814832253904 14.930730528477449 6 33.665561230464846 35.56048646541022 16.474730885758888 14.29922141836604 7 29.702527976588843 31.970862807678756 19.73728580329984 18.589323412432563 8 26.600381850691075 35.349571115471775 18.967466913564174 19.08258012027298 9 26.18048649935193 16.627371366591223 19.70583808177854 37.48630405227831 10-11 25.53904598015108 26.07787668290949 26.709328331090433 21.673749005849 12-13 26.173787066892025 23.219819573433874 27.37735991383393 23.22903344584017 14-15 22.754923738414202 24.9166359824623 25.467108886616323 26.861331392507175 16-17 22.58213738110012 28.187479430241584 26.415728961238177 22.81465422742011 18-19 22.610033442051286 26.40959331172752 28.66452927789763 22.315843968323566 20-21 22.820867509337013 25.71664121800849 29.125377897935095 22.337113374719408 22-23 22.755715959219227 25.582687013193233 29.019168643487202 22.642428384100334 24-25 22.673734311615906 25.83769262909021 28.944868442671375 22.543704616622513 26-27 22.163089750230526 26.284112971865213 28.865198003249827 22.68759927465444 28-29 22.38538768306385 26.53956535187457 28.769880498287765 22.305166466773812 30-31 22.398081997335037 26.02976307238648 28.68324005499395 22.888914875284534 32-33 22.232274580599647 26.03447723545563 28.748924007270766 22.984324176673958 34-35 22.91601463221295 26.14537441519292 28.482639103602125 22.455971848992007 36-37 22.925349813219952 25.813589912689004 28.45441469700365 22.806645577087394 38-39 22.513984745542572 26.05367482628667 28.571256313677228 22.861084114493526 40-41 22.938690237485925 25.853036548275117 28.475073142027142 22.73320007221182 42-43 22.58267607487338 25.906892652875896 28.357960860077846 23.152470412172885 44-45 22.66095826588811 26.245714307366963 28.504777688252698 22.58854973849223 46-47 23.027710361142667 26.55221492063514 27.771242784621247 22.64883193360095 48-49 22.86988781255581 26.365728553608463 27.85502242939438 22.909361204441343 50-51 22.95337505007352 26.729753850107002 27.707250990362116 22.60962010945736 52-53 23.233872881627402 26.471110979457023 27.636037228866734 22.658978910048837 54-55 22.787112014166286 26.650738435901246 27.68524102973549 22.87690852019698 56-57 22.804781982556676 26.845125310386948 27.64347721555743 22.706615491498955 58-59 22.84237802641264 26.42140773503727 27.574140672926127 23.162073565623956 60-61 22.596617354940065 26.67579672440808 27.743142037268132 22.98444388338372 62-63 22.557832979876558 26.757240467603165 27.925972821315288 22.758953731204993 64-65 22.352561680417438 26.68606115049061 27.7583836766692 23.202993492422753 66-67 22.340935053420775 26.758970930425807 27.70927635806325 23.190817658090168 68-69 22.691455192070887 26.40465344573707 27.895323304256475 23.008568057935573 70-71 22.568062961576256 26.657627312466698 27.46167976299509 23.312629962961957 72-73 22.59546346811535 26.754416028211324 27.36668215515748 23.28343834851584 74-75 22.576346835646216 27.09927318907796 27.121524260384376 23.20285571489144 76-77 22.686741082607618 26.773136550277947 27.406672083619938 23.13345028349449 78-79 22.72189157528285 26.88769856756145 27.416833176553983 22.973576680601717 80-81 22.790389036196288 27.024007087288414 27.18916818738879 22.99643568912651 82-83 22.743521216365213 27.036239579196387 27.170212113707358 23.050027090731042 84-85 22.667322510870598 27.0488888108122 27.13471002058641 23.14907865773079 86-87 22.874344992492794 27.022975594953312 27.06401635981677 23.038663052737125 88-89 22.694051108592845 27.242881204727148 26.78539075443558 23.277676932244432 90-91 22.66812187664908 27.197006543278086 26.938923204318648 23.195948375754185 92-93 22.691020591141303 26.798829366552145 27.153091857318845 23.357058184987707 94-95 22.496432418133338 26.795148984216528 27.17369327303721 23.534725324612925 96-97 22.875542113024043 26.50059950613508 27.01983278583836 23.60402559500252 98-99 22.503809557925948 26.79901723049961 26.793230560232352 23.903942651342096 100-101 22.86457761040466 26.361283442427062 26.961181277924307 23.81295766924397 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 754.0 1 516.0 2 412.0 3 551.0 4 438.0 5 391.0 6 812.0 7 1883.5 8 3087.0 9 3608.0 10 3054.0 11 2370.0 12 2424.0 13 2595.0 14 2799.0 15 3092.0 16 3283.5 17 3449.0 18 3695.0 19 4142.0 20 4717.5 21 5507.0 22 6689.5 23 8225.0 24 10222.0 25 12887.0 26 16687.5 27 20915.0 28 24611.5 29 28362.0 30 32702.5 31 38172.5 32 45865.0 33 49907.5 34 50974.0 35 53825.0 36 57949.0 37 63082.5 38 67727.0 39 71264.0 40 75525.0 41 81963.0 42 86171.0 43 89405.5 44 93073.0 45 97940.0 46 101621.5 47 104379.5 48 105728.5 49 104455.5 50 103838.5 51 102495.5 52 102856.0 53 106018.0 54 109264.5 55 104342.0 56 95247.5 57 88707.0 58 83130.0 59 74177.5 60 60385.5 61 49641.5 62 41410.0 63 32116.0 64 23754.5 65 17673.5 66 13315.5 67 10383.0 68 7891.5 69 5900.5 70 4647.0 71 3433.5 72 2502.0 73 2054.5 74 1569.0 75 1144.5 76 790.5 77 433.5 78 258.5 79 139.0 80 71.5 81 42.0 82 20.5 83 14.0 84 8.5 85 7.0 86 6.0 87 2.5 88 1.0 89 0.5 90 0.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.00909331706639947 3 0.005407768103881503 4 0.0 5 1.3777753130908286E-4 6 1.0333314848181215E-4 7 0.0 8 6.888876565454143E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.7222191413635358E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016533303757089945 26-27 0.021166073247357857 28-29 3.099994454454365E-4 30-31 8.61109570681768E-5 32-33 0.0033755495170725304 34-35 0.015758305143476356 36-37 0.0074916532649313815 38-39 0.020804407227671518 40-41 0.022698848283171404 42-43 0.01780774592169896 44-45 0.008886650769435846 46-47 0.001963329821154431 48-49 3.2722163685907187E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2388848837725966E-4 68-69 2.066662969636243E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.722219141363536E-4 82-83 8.266651878544972E-4 84-85 0.0035305492397952483 86-87 6.888876565454144E-4 88-89 3.4444382827270716E-5 90-91 0.0034099938998998014 92-93 5.683323166499668E-4 94-95 1.3777753130908286E-4 96-97 2.755550626181657E-4 98-99 2.4111067979089502E-4 100-101 0.0024111067979089504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2903231.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.53182830472026 #Duplication Level Percentage of deduplicated Percentage of total 1 72.31456871935835 35.09558233017794 2 14.779962750870201 14.34597229150787 3 5.315078444630252 7.738514235027448 4 2.4535444508787467 4.762999921121859 5 1.3541786274816137 3.28603823214297 6 0.818638693169647 2.383801950030593 7 0.5224712433658748 1.7749539274030441 8 0.3919726288282998 1.521851865795591 9 0.2940330210786123 1.2842964085414863 >10 1.5648609860149882 13.95034796260336 >50 0.12299308710146692 4.131961000311151 >100 0.061004944719729816 5.3728843938495805 >500 0.003844571650312693 1.2994199850272135 >1k 0.0026342435381052653 2.20570037925084 >5k 1.4239154260028462E-4 0.4915454225706882 >10k+ 7.119577130014231E-5 0.35412969463847765 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10249 0.35302047959669763 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8731 0.30073390646490067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5495 0.18927188363585262 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3429 0.1181097887147113 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3410 0.11745534544099316 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3006 0.10353981477877579 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2944 0.10140426304348502 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.444438282727072E-5 2 0.0 0.0 0.0 0.0 3.444438282727072E-5 3 0.0 0.0 0.0 0.0 3.444438282727072E-5 4 0.0 0.0 0.0 3.444438282727072E-5 3.444438282727072E-5 5 0.0 0.0 0.0 3.444438282727072E-5 6.888876565454144E-5 6 0.0 0.0 0.0 3.444438282727072E-5 1.377775313090829E-4 7 0.0 0.0 0.0 3.444438282727072E-5 1.722219141363536E-4 8 0.0 0.0 0.0 3.444438282727072E-5 1.722219141363536E-4 9 0.0 0.0 0.0 3.444438282727072E-5 1.722219141363536E-4 10-11 0.0 0.0 0.0 3.444438282727072E-5 1.8944410554998895E-4 12-13 0.0 0.0 0.0 3.444438282727072E-5 4.133325939272486E-4 14-15 0.0 0.0 0.0 3.444438282727072E-5 4.822213595817901E-4 16-17 0.0 0.0 0.0 8.61109570681768E-5 5.166657424090608E-4 18-19 0.0 0.0 0.0 1.0333314848181215E-4 6.19998890890873E-4 20-21 0.0 0.0 0.0 1.377775313090829E-4 6.544432737181436E-4 22-23 0.0 0.0 0.0 2.9277725403180116E-4 6.71665465131779E-4 24-25 3.444438282727072E-5 0.0 0.0 4.133325939272486E-4 6.888876565454144E-4 26-27 3.444438282727072E-5 0.0 0.0 7.233320393726852E-4 7.405542307863204E-4 28-29 3.444438282727072E-5 0.0 0.0 0.0028588837746634697 7.922208050272266E-4 30-31 3.444438282727072E-5 0.0 0.0 0.010350537039594852 7.922208050272266E-4 32-33 3.444438282727072E-5 0.0 0.0 0.02684939641385753 8.438873792681326E-4 34-35 3.444438282727072E-5 0.0 0.0 0.05147713013535609 9.299983363363094E-4 36-37 3.444438282727072E-5 0.0 0.0 0.09305150020787184 9.299983363363094E-4 38-39 3.444438282727072E-5 0.0 0.0 0.15412139096062283 9.299983363363094E-4 40-41 3.444438282727072E-5 0.0 0.0 0.21768849946835095 9.644427191635802E-4 42-43 5.1666574240906084E-5 0.0 0.0 0.2831844934144062 9.816649105772156E-4 44-45 6.888876565454144E-5 0.0 0.0 0.3651104579690696 9.988871019908508E-4 46-47 1.377775313090829E-4 0.0 0.0 0.4632597268353775 0.0010849980590590276 48-49 1.377775313090829E-4 0.0 0.0 0.5613917735102718 0.001239997781781746 50-51 1.377775313090829E-4 0.0 0.0 0.6696676909277973 0.0012744421646090167 52-53 1.377775313090829E-4 0.0 0.0 0.7732075057065732 0.0013605531216771935 54-55 1.377775313090829E-4 0.0 0.0 0.8862023035714347 0.0013777753130908288 56-57 1.377775313090829E-4 0.0 0.0 1.0134915203096138 0.001394997504504464 58-59 1.377775313090829E-4 0.0 0.0 1.1465329489799467 0.0014466640787453703 60-61 1.5499972272271824E-4 0.0 0.0 1.2927665762731246 0.0014466640787453703 62-63 1.722219141363536E-4 0.0 0.0 1.4303374412852439 0.0015155528443999116 64-65 1.722219141363536E-4 0.0 0.0 1.577552733488999 0.0015499972272271824 66-67 2.066662969636243E-4 0.0 0.0 1.7453485444320482 0.0015844416100544532 68-69 2.066662969636243E-4 0.0 0.0 1.930642101851351 0.001618885992881724 70-71 2.066662969636243E-4 0.0 0.0 2.11894954276804 0.0016361081842953592 72-73 2.238884883772597E-4 0.0 0.0 2.312974751234056 0.0016877747585362652 74-75 2.4111067979089504E-4 0.0 0.0 2.5072582925712767 0.0017566635241908068 76-77 2.4111067979089504E-4 0.0 0.0 2.7187640253221326 0.0017911079070180775 78-79 2.4111067979089504E-4 0.0 0.0 2.952520829379405 0.0017911079070180775 80-81 2.4111067979089504E-4 0.0 0.0 3.1992459435711456 0.001825552289845348 82-83 2.4111067979089504E-4 0.0 0.0 3.453497155410644 0.0019977742039817017 84-85 2.4111067979089504E-4 0.0 0.0 3.7100044743253293 0.0020322185868089726 86-87 2.4111067979089504E-4 0.0 0.0 3.9756223324978275 0.0021011073524635137 88-89 2.4111067979089504E-4 0.0 0.0 4.2558962755633285 0.0021699961181180553 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4750 0.0 56.90387 1 GTATCAA 8515 0.0 42.56602 1 ATCAACG 10845 0.0 33.11143 3 TATCAAC 11055 0.0 32.657127 2 TCAACGC 11095 0.0 32.57772 4 CAACGCA 11375 0.0 31.734047 5 AACGCAG 11780 0.0 30.723661 6 GTGGTAT 2205 0.0 27.144705 1 CGCAGAG 13340 0.0 27.05958 8 ACGCAGA 13355 0.0 26.993624 7 TGGTATC 2075 0.0 26.327091 2 TAAGGTG 1575 0.0 23.823736 5 GCAGAGT 15165 0.0 23.552591 9 GTACATG 8725 0.0 23.193554 1 TACATGG 8540 0.0 23.0285 2 GAGTACT 9950 0.0 22.817501 12-13 ACATGGG 8490 0.0 22.099129 3 GTAAGGT 1485 0.0 22.069166 4 CAGAGTA 14320 0.0 21.426579 10-11 GTACTTT 10545 0.0 21.282299 14-15 >>END_MODULE