FastQCFastQC Report
Thu 26 May 2016
SRR937949_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937949_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914326
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106460.36529887184892834No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87120.29893704410556676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55180.18934051990065626No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36380.1248316077199325No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35150.1206110778272575No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30380.10424365702395684No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30370.10420934377279686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.055.1882821
GTATCAA86700.042.6644131
ATCAACG108600.033.5172353
TCAACGC110200.032.9443554
TATCAAC111800.032.7273372
CAACGCA112050.032.4004255
AACGCAG115600.031.4465396
TGGTATC20550.027.9792022
ACGCAGA132150.027.3019127
GTGGTAT23250.026.6872331
CGCAGAG134500.026.5774928
GCAGAGT149700.023.7514869
GTACATG83650.023.1085221
TACATGG82100.022.80422
GAGTACT100350.021.6220612-13
CAGAGTA141200.021.42710710-11
ACATGGG85150.021.1506543
AGAGTAC138350.020.90649610-11
TAAGGTG15400.020.6739435
ATAACGC1856.5348813E-620.5488473
Produced by FastQC (version 0.11.5)