FastQCFastQC Report
Thu 26 May 2016
SRR937949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914326
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104280.3578185830960572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88500.3036722727656412No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56740.19469338708160994No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34220.11741994546938127No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA33220.1139886203533853No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30390.10427797027511677No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29630.10167016318695986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.052.553471
GTATCAA84950.042.4991871
ATCAACG108000.033.161433
TCAACGC109200.032.882894
TATCAAC110800.032.3267482
CAACGCA111550.032.1049925
AACGCAG114950.031.1967096
TGGTATC21650.028.0855222
ACGCAGA131550.027.1156397
CGCAGAG132550.026.9110728
TACATGG80350.026.2498912
GTGGTAT24150.025.964931
GTACATG83650.025.9525851
TAAGGTG14750.025.439345
ACATGGG82300.024.6441443
GCAGAGT151350.023.2230959
GTAAGGT15400.021.89824
CATGGGG55650.021.8497054
AGAGTAC135450.021.53061710-11
GAGTACT102000.021.42044412-13
Produced by FastQC (version 0.11.5)