##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937949_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2914326 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3629137577608 33.0 31.0 34.0 31.0 34.0 2 32.602051726539855 34.0 31.0 34.0 31.0 34.0 3 32.66440233522262 34.0 31.0 34.0 31.0 34.0 4 36.16472934050618 37.0 35.0 37.0 35.0 37.0 5 36.11685000236761 37.0 35.0 37.0 35.0 37.0 6 36.15410218348942 37.0 36.0 37.0 35.0 37.0 7 36.11370759482639 37.0 36.0 37.0 35.0 37.0 8 36.104860952412324 37.0 36.0 37.0 35.0 37.0 9 37.79107999585496 39.0 38.0 39.0 35.0 39.0 10-11 37.76302290821274 39.0 38.0 39.0 35.0 39.0 12-13 37.7079055328745 39.0 37.0 39.0 35.0 39.0 14-15 38.98783029077735 40.0 38.0 41.0 35.5 41.0 16-17 38.927821046787486 40.0 38.0 41.0 35.0 41.0 18-19 38.94195656216909 40.0 38.0 41.0 35.0 41.0 20-21 38.84819491710948 40.0 38.0 41.0 35.0 41.0 22-23 38.68914236087521 40.0 38.0 41.0 34.0 41.0 24-25 38.64606018681506 40.0 38.0 41.0 34.0 41.0 26-27 38.60649632196261 40.0 38.0 41.0 34.0 41.0 28-29 38.551645903718395 40.0 38.0 41.0 34.0 41.0 30-31 38.34701196777574 40.0 38.0 41.0 34.0 41.0 32-33 38.16823152248581 40.0 38.0 41.0 34.0 41.0 34-35 37.959522201702896 40.0 37.0 41.0 33.0 41.0 36-37 37.81693897662787 40.0 37.0 41.0 33.0 41.0 38-39 37.81005316495134 40.0 37.0 41.0 33.0 41.0 40-41 37.745609104815316 40.0 37.0 41.0 33.0 41.0 42-43 37.583242231651504 40.0 37.0 41.0 33.0 41.0 44-45 37.37874949473738 40.0 37.0 41.0 32.5 41.0 46-47 37.0570536721012 40.0 36.5 41.0 31.5 41.0 48-49 36.70014421859462 39.5 36.0 41.0 31.0 41.0 50-51 36.389220354895095 39.0 35.5 41.0 30.0 41.0 52-53 35.930600591697704 39.0 35.0 40.0 29.0 41.0 54-55 35.68203968945135 39.0 35.0 40.0 28.0 41.0 56-57 35.41727486904348 38.0 35.0 40.0 28.0 41.0 58-59 35.04972093032832 38.0 34.0 40.0 27.5 41.0 60-61 34.65844435385746 37.5 34.0 40.0 26.0 41.0 62-63 34.264563230057306 37.0 34.0 40.0 26.0 41.0 64-65 33.843392777609644 36.5 33.0 39.0 25.5 41.0 66-67 33.394860252422006 36.0 33.0 39.0 24.5 40.0 68-69 32.909108658399916 35.0 32.0 38.5 23.0 40.0 70-71 32.3640793789027 35.0 31.5 38.0 22.0 40.0 72-73 31.929463622120515 35.0 31.0 37.0 20.5 39.0 74-75 31.465659126672854 35.0 31.0 36.5 20.0 39.0 76-77 30.219286037320465 33.5 29.5 35.0 19.5 37.5 78-79 30.978148292263803 34.0 31.0 36.0 20.0 38.0 80-81 30.821079385079088 34.0 31.0 35.5 18.5 37.0 82-83 30.42616800591286 34.0 30.5 35.0 17.5 37.0 84-85 30.01540785073461 34.0 30.0 35.0 12.0 36.0 86-87 29.530267032583176 34.0 29.5 35.0 7.0 36.0 88-89 28.99987715856085 34.0 29.0 35.0 3.5 35.5 90-91 28.5984114337243 34.0 29.0 35.0 2.0 35.0 92-93 28.11130772604026 33.0 27.5 35.0 2.0 35.0 94-95 27.597779898336697 33.0 27.0 35.0 2.0 35.0 96-97 26.889784807876676 33.0 25.5 35.0 2.0 35.0 98-99 26.037034806675713 32.5 24.5 34.0 2.0 35.0 100-101 23.45052887014013 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1919027361458987 1101 2 0.1909259589479433 1101 3 0.19549257721192959 1101 4 0.14949742461455884 1101 5 0.10958002129906674 1101 6 0.10006891672103535 1101 7 0.1315709549891082 1101 8 0.07607544104474329 1101 9 0.07858367116946852 1101 10-11 0.09813414849839575 1101 12-13 0.16158167664512035 1101 14-15 0.21092434119417902 1101 16-17 0.19830721089703474 1101 18-19 0.2534613665640535 1101 20-21 0.21471455345013624 1101 22-23 0.22209089822351302 1101 24-25 0.2390048499328543 1101 26-27 0.2812191480240429 1101 28-29 0.2473537745035017 1101 30-31 0.2941463135699962 1101 32-33 0.24755733262586688 1101 34-35 0.23072298357048737 1101 36-37 0.34515313016436977 1101 38-39 0.2706153099549695 1101 40-41 0.3251417937576946 1101 42-43 0.414884479809686 1101 44-45 0.5500676510548956 1101 46-47 0.5180095115204182 1101 48-49 0.5112110934326424 1101 50-51 0.46512840767680785 1101 52-53 0.41694363900557363 1101 54-55 0.4862930378301513 1101 56-57 0.4879664811268327 1101 58-59 0.4888213770374179 1101 60-61 0.6231882272800959 1101 62-63 0.5968868577674584 1101 64-65 0.675156610291225 1101 66-67 0.501602179509149 1101 68-69 0.5284352042339222 1101 70-71 0.6124395091511019 1101 72-73 0.621608983799181 1101 74-75 0.7335689540047525 1101 76-77 0.566770373433247 1101 78-79 0.6592905944232328 1101 80-81 0.7917129275293284 1101 82-83 0.8325418478531326 1101 84-85 0.9187096983711314 1101 86-87 1.0517636862923823 1101 88-89 1.058139801703863 1101 90-91 1.174117745391765 1101 92-93 1.4283156206510732 1101 94-95 1.24812042784718 1101 96-97 1.283751930604101 1101 98-99 1.498773994887049 1101 100-101 1.5206132448527718 1102 1 0.20687460279972214 1102 2 0.2149983796053121 1102 3 0.2406292872364304 1102 4 0.11575783925411542 1102 5 0.134810619052665 1102 6 0.13605593717242215 1102 7 0.1662851055805774 1102 8 0.14652227086305203 1102 9 0.16218008453149935 1102 10-11 0.19191300352325413 1102 12-13 0.18529249830175587 1102 14-15 0.24960062895018353 1102 16-17 0.2569862657614195 1102 18-19 0.29344278556051506 1102 20-21 0.24835722536482763 1102 22-23 0.30800754490191906 1102 24-25 0.3316652242295177 1102 26-27 0.12312267291929402 1102 28-29 0.09397127542964512 1102 30-31 0.08869523934360757 1102 32-33 0.19462067419372175 1102 34-35 0.3292654027602566 1102 36-37 0.29493825902563486 1102 38-39 0.1187049384060117 1102 40-41 0.2646744627695128 1102 42-43 0.24542630937261123 1102 44-45 0.05507463024533621 1102 46-47 0.009293249392499092 1102 48-49 0.01537332873900965 1102 50-51 0.04787652129117248 1102 52-53 0.13296118816215596 1102 54-55 0.13690513125755643 1102 56-57 0.09032932015418282 1102 58-59 0.0679848568960395 1102 60-61 0.11945638077185095 1102 62-63 0.19841542654529576 1102 64-65 0.12213480508918906 1102 66-67 0.028985949642610365 1102 68-69 0.24957778725202218 1102 70-71 0.2839062899502167 1102 72-73 0.1546110830517442 1102 74-75 0.17174528734045325 1102 76-77 0.1613022957137069 1102 78-79 0.15683769506904355 1102 80-81 0.25042954524360894 1102 82-83 0.36496149665018507 1102 84-85 0.391637421887868 1102 86-87 0.4074685522007684 1102 88-89 0.41836171345249795 1102 90-91 0.4204711426315093 1102 92-93 0.5694577381743038 1102 94-95 0.6014577351274575 1102 96-97 0.7009728486504976 1102 98-99 0.8666396556537812 1102 100-101 1.0265827639901026 1103 1 0.2003626306817381 1103 2 0.21393080220595095 1103 3 0.20269866226611555 1103 4 0.11216007841645137 1103 5 0.07424510305275334 1103 6 0.1424562761970023 1103 7 0.19923999481004273 1103 8 0.17198649201908012 1103 9 0.12310750176925467 1103 10-11 0.19298170600418274 1103 12-13 0.23879781493721453 1103 14-15 0.2716746668179226 1103 16-17 0.19726268580186712 1103 18-19 0.23866856442948148 1103 20-21 0.2587252895494814 1103 22-23 0.31207496478985775 1103 24-25 0.2765622292623888 1103 26-27 0.2837748201999659 1103 28-29 0.3250741553884424 1103 30-31 0.2568054898367649 1103 32-33 0.3266595595129047 1103 34-35 0.3289987584285399 1103 36-37 0.3342501091347643 1103 38-39 0.34820638976124485 1103 40-41 0.33307910960640186 1103 42-43 0.3385178085063032 1103 44-45 0.36229371612906647 1103 46-47 0.19736875986701108 1103 48-49 0.3287533612913691 1103 50-51 0.3536655034923797 1103 52-53 0.3417590135044932 1103 54-55 0.4336640212770888 1103 56-57 0.47672677146940146 1103 58-59 0.4705731695487074 1103 60-61 0.36374102890884785 1103 62-63 0.5200693714563087 1103 64-65 0.5143645542460078 1103 66-67 0.4191532992754645 1103 68-69 0.49472018507587023 1103 70-71 0.32896918200988523 1103 72-73 0.30482197334128713 1103 74-75 0.3319794043139339 1103 76-77 0.3707973591058007 1103 78-79 0.37085339870755263 1103 80-81 0.4802497072053491 1103 82-83 0.5821308664294911 1103 84-85 0.7163658396981596 1103 86-87 0.6976839053138697 1103 88-89 0.5673002614972305 1103 90-91 0.6094137784726072 1103 92-93 0.6819550192271819 1103 94-95 0.47827255420184045 1103 96-97 0.7645578335619021 1103 98-99 0.6471341790067271 1103 100-101 0.6414468416357337 1104 1 0.121813691469292 1104 2 0.076298292535526 1104 3 0.08820959682918783 1104 4 0.022467780193345277 1104 5 -0.05016390026619888 1104 6 -0.0158022898814707 1104 7 0.07345109176995379 1104 8 0.06686673026425183 1104 9 -0.004843220494265665 1104 10-11 0.04630397615110127 1104 12-13 0.09068017428402442 1104 14-15 0.1285631574741899 1104 16-17 0.12219415243949783 1104 18-19 0.12214575075027057 1104 20-21 0.19823400358426113 1104 22-23 0.17728274279384237 1104 24-25 0.13989486818744723 1104 26-27 0.2397550958484942 1104 28-29 0.173512683684784 1104 30-31 0.10002163388229945 1104 32-33 0.10483174277987217 1104 34-35 0.186828843035876 1104 36-37 0.15534441766983065 1104 38-39 0.18612356737035896 1104 40-41 0.22324048851186262 1104 42-43 0.28275769184570976 1104 44-45 0.33422001258142586 1104 46-47 0.2641301974815349 1104 48-49 0.316250816442853 1104 50-51 0.3836486719826624 1104 52-53 0.3266635639303743 1104 54-55 0.38157298435192644 1104 56-57 0.5130635389673088 1104 58-59 0.40069328639867763 1104 60-61 0.48515740873754964 1104 62-63 0.19352861363592666 1104 64-65 0.2796962604544859 1104 66-67 0.49967644911140496 1104 68-69 0.532706606237646 1104 70-71 0.4084946328623502 1104 72-73 0.4188489416336587 1104 74-75 0.2980997768324123 1104 76-77 0.32314619510392717 1104 78-79 0.42411494758521684 1104 80-81 0.37416195609390357 1104 82-83 0.3714519718643423 1104 84-85 0.5016660844770762 1104 86-87 0.489568679307645 1104 88-89 0.4134122064243435 1104 90-91 0.4106406321876115 1104 92-93 0.596867594803264 1104 94-95 0.503148152537154 1104 96-97 0.7006151411831887 1104 98-99 0.8486960058055182 1104 100-101 0.9724284192997779 1105 1 0.11231868520223998 1105 2 0.1190930556828107 1105 3 0.08797198114816496 1105 4 0.04586979236410116 1105 5 0.03667814258427882 1105 6 0.025694089276186105 1105 7 0.040536173845815426 1105 8 -0.013684314372149231 1105 9 -0.002992637351376004 1105 10-11 -0.003958564564641165 1105 12-13 0.05389829608197516 1105 14-15 0.037379929829860714 1105 16-17 0.015167418214211636 1105 18-19 0.04909313655267056 1105 20-21 0.11157945785308954 1105 22-23 0.11512845459152743 1105 24-25 0.07934325524235675 1105 26-27 0.10836704885696946 1105 28-29 0.027707047942023166 1105 30-31 0.06532963258100466 1105 32-33 0.1262321448918371 1105 34-35 0.14678008483511462 1105 36-37 0.1863759740167481 1105 38-39 0.02785306496310369 1105 40-41 0.0756614611610047 1105 42-43 0.09999017619148987 1105 44-45 0.141697276877629 1105 46-47 0.15496816640892064 1105 48-49 0.23127266947822278 1105 50-51 0.2089117608701656 1105 52-53 0.2248633678415004 1105 54-55 0.2483953243428232 1105 56-57 0.1577720480698659 1105 58-59 0.22993677355869835 1105 60-61 0.18515832608622418 1105 62-63 0.3271989570062459 1105 64-65 0.2590563627679572 1105 66-67 0.19679196926377074 1105 68-69 0.2534676834466296 1105 70-71 0.42663486728297784 1105 72-73 0.3600796700398341 1105 74-75 0.28778318414283177 1105 76-77 0.4072354088287149 1105 78-79 0.44195894550708914 1105 80-81 0.37684934022269445 1105 82-83 0.5529534344761196 1105 84-85 0.4722227719265355 1105 86-87 0.4872199310858498 1105 88-89 0.3381930831501343 1105 90-91 0.3903857972007394 1105 92-93 0.5025952514338385 1105 94-95 0.4787439928755255 1105 96-97 0.6215623954243625 1105 98-99 0.8291160933250588 1105 100-101 0.911097283725038 1106 1 -0.09042498545798594 1106 2 -0.021036108817298782 1106 3 0.03680798017825282 1106 4 -0.02029432164358269 1106 5 -0.03608892911226036 1106 6 -0.04030157627916253 1106 7 0.051556692197166853 1106 8 -0.08402439849884757 1106 9 -0.12634633078317137 1106 10-11 -0.03595180180256108 1106 12-13 -0.10119540780249281 1106 14-15 -0.2651871802551895 1106 16-17 -0.14715416495300815 1106 18-19 0.018637656370408706 1106 20-21 0.009723000758462774 1106 22-23 0.011881965301085984 1106 24-25 0.061776076448062156 1106 26-27 0.015594174554536266 1106 28-29 -0.03161539918843914 1106 30-31 0.09142661988043699 1106 32-33 0.035793500689102586 1106 34-35 0.07101706875670999 1106 36-37 -0.07569346841444258 1106 38-39 -0.02654104590168771 1106 40-41 0.050388208957755865 1106 42-43 -0.09273380373122819 1106 44-45 0.05600411371269587 1106 46-47 -0.0015801785185445283 1106 48-49 -0.08999232963742543 1106 50-51 -0.0917924423176757 1106 52-53 -0.1476141719954498 1106 54-55 -0.5344317327341628 1106 56-57 -0.2599688099444961 1106 58-59 -0.06859400130860394 1106 60-61 -0.15732862913264256 1106 62-63 -0.07616537970802284 1106 64-65 -0.15158018176914823 1106 66-67 -0.2047761750061312 1106 68-69 -0.33725089548676834 1106 70-71 -0.44318495493298116 1106 72-73 -0.20178878679060475 1106 74-75 -0.2226455481983436 1106 76-77 -0.04508769480742103 1106 78-79 -0.004177556252976444 1106 80-81 -0.27063415072076324 1106 82-83 -0.35985132977805634 1106 84-85 -0.21067589790695962 1106 86-87 0.04459790680533615 1106 88-89 0.01683288849464759 1106 90-91 -0.07389692490852084 1106 92-93 0.01064886059707959 1106 94-95 -0.013561902421347583 1106 96-97 0.043878512882582044 1106 98-99 0.16788443813726772 1106 100-101 0.31532624861373293 1107 1 0.06194804333235737 1107 2 0.07468238116282322 1107 3 0.07386548769375878 1107 4 0.04849391888198795 1107 5 -2.710521929003562E-4 1107 6 0.045248738737683425 1107 7 0.0711266620485489 1107 8 0.09123893099392433 1107 9 0.05138651778229075 1107 10-11 0.054796908232553676 1107 12-13 0.09742519465883959 1107 14-15 0.14798184898372568 1107 16-17 0.15536852444353855 1107 18-19 0.13808686723706387 1107 20-21 0.1567771569848233 1107 22-23 0.12219631535666053 1107 24-25 0.11279404134959492 1107 26-27 0.0707233139270329 1107 28-29 0.13093175808271695 1107 30-31 0.0822824499328334 1107 32-33 0.26768365807412664 1107 34-35 0.2724082217863568 1107 36-37 0.20628146706025774 1107 38-39 0.14808190159608614 1107 40-41 0.3187381406979739 1107 42-43 0.3613216025173287 1107 44-45 0.22297159538344147 1107 46-47 0.24051863774897697 1107 48-49 0.2963064183053703 1107 50-51 0.261793350467876 1107 52-53 0.308234000750943 1107 54-55 0.3817372198667428 1107 56-57 0.25395311194622394 1107 58-59 0.24594234524067105 1107 60-61 0.31776087929732455 1107 62-63 0.4961344619500565 1107 64-65 0.398672160777636 1107 66-67 0.2796774587828281 1107 68-69 0.34130765183763856 1107 70-71 0.3173986673083178 1107 72-73 0.32938101662208297 1107 74-75 0.38301390886683606 1107 76-77 0.35491117943653805 1107 78-79 0.39134925211173766 1107 80-81 0.5212775302538297 1107 82-83 0.628858960801228 1107 84-85 0.7278794713215397 1107 86-87 0.6442766766484098 1107 88-89 0.585547088911266 1107 90-91 0.5691034271067359 1107 92-93 0.6209036754773258 1107 94-95 0.5742604913776397 1107 96-97 0.7684243027931217 1107 98-99 0.7718730134453686 1107 100-101 1.0002928374733386 1108 1 0.027825037555913923 1108 2 0.0332004214755699 1108 3 0.06645843190823797 1108 4 0.049303000853491596 1108 5 0.08033313267019793 1108 6 0.0746035406972041 1108 7 0.044031804307735456 1108 8 0.08981865985125381 1108 9 0.08682156548331221 1108 10-11 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60-61 -0.22796149933213883 2316 62-63 -0.4800651094474091 2316 64-65 -0.5030266577808362 2316 66-67 -0.42221231891202393 2316 68-69 -0.3286384401046192 2316 70-71 -0.43589732799832603 2316 72-73 -0.3330988103284831 2316 74-75 -0.4023571093748046 2316 76-77 -0.2660603176304157 2316 78-79 -0.18149010798817855 2316 80-81 -0.3057104864471505 2316 82-83 -0.416112388291495 2316 84-85 -0.3264401397475005 2316 86-87 -0.2858520931006723 2316 88-89 -0.3517875332990634 2316 90-91 -0.25291528922083373 2316 92-93 -0.4893071194438612 2316 94-95 -0.6004483200655919 2316 96-97 -0.5183517669590323 2316 98-99 -0.2989580890491048 2316 100-101 -0.22611919556593207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 21.0 5 42.0 6 95.0 7 277.0 8 699.0 9 1343.0 10 2201.0 11 3371.0 12 5381.0 13 7791.0 14 10423.0 15 14044.0 16 17888.0 17 24197.0 18 24566.0 19 18384.0 20 16833.0 21 16478.0 22 17281.0 23 18911.0 24 21750.0 25 25999.0 26 30715.0 27 37431.0 28 45787.0 29 56138.0 30 67932.0 31 84295.0 32 106417.0 33 139218.0 34 193236.0 35 287871.0 36 457772.0 37 660279.0 38 461894.0 39 37351.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14050658711482 18.620806320226357 14.690017520346041 23.548669572312775 2 16.053253459093163 19.625390454213818 39.74177000419367 24.579586082499347 3 19.6872631164634 23.011773958930636 29.451677560092282 27.849285364513683 4 13.304624570011924 16.37943606152368 35.84068352019764 34.47525584826675 5 14.046854760536318 36.8274300223894 34.18812246403541 14.937592753038869 6 33.685627482992636 35.569424971674415 16.459620509167472 14.28532703616548 7 29.659344905134155 32.04456193301642 19.736810500952878 18.55928266089655 8 26.643070498556266 35.320341143630664 18.93172151946512 19.10486683834795 9 26.190141226866796 16.594963212662446 19.658458685702797 37.556436874767954 10-11 25.545649319945674 26.095690736039824 26.666663235341552 21.69199670867295 12-13 26.222975741217695 23.252889347313925 27.32705606716613 23.19707884430225 14-15 22.760013807652264 24.928353931578005 25.462851444896696 26.848780815873035 16-17 22.56053476680814 28.1692417125522 26.43792696539189 22.832296555247762 18-19 22.61896918875925 26.463374378844374 28.653143128119503 22.26451330427687 20-21 22.828537370218704 25.716683720352496 29.152109269862052 22.302669639566748 22-23 22.80096667291168 25.585349751537752 28.982241519994673 22.631442055555897 24-25 22.72051420543648 25.796402813017515 28.953978599709146 22.529104381836863 26-27 22.1575867443711 26.248944422889114 28.86567739435606 22.727791438383726 28-29 22.39574433333814 26.518121171070085 28.73981840054956 22.346316095042216 30-31 22.430023271246938 26.01701045113004 28.706139944536062 22.846826333086963 32-33 22.200850697625583 26.01155880938018 28.756252757938917 23.031337735055317 34-35 22.923148096986278 26.153221270153853 28.505583653908523 22.418046978951345 36-37 22.951278135477164 25.793113813601593 28.462759659470414 22.792848391450836 38-39 22.496572786734074 26.079956126179106 28.541131964484308 22.882339122602517 40-41 22.921445485431896 25.87602525824496 28.478430968804698 22.724098287518444 42-43 22.561208497864005 25.890507419622615 28.382909010921736 23.165375071591647 44-45 22.616922389848646 26.216697495051474 28.56519075309732 22.601189362002557 46-47 23.045602997056612 26.539738519300858 27.755800140409825 22.65885834323271 48-49 22.89117621021121 26.380199058032627 27.863457965924198 22.86516676583196 50-51 22.937018713760917 26.739149978416965 27.713509058355175 22.610322249466943 52-53 23.24986978121185 26.453818138396322 27.62594164139496 22.670370438996873 54-55 22.795064793712168 26.622364828094042 27.708344914055598 22.874225464138192 56-57 22.79899366096998 26.838950069415706 27.686521686317867 22.675534583296447 58-59 22.859762428774268 26.426298910966032 27.559699910030655 23.15423875022904 60-61 22.59743762365638 26.721032581804504 27.735040623458048 22.946489171081065 62-63 22.575614395918645 26.741328869865622 27.916832228103512 22.76622450611222 64-65 22.37052409373557 26.701353932264272 27.75681238131904 23.171309592681123 66-67 22.337154456982507 26.769551518944688 27.712771323455232 23.18052270061757 68-69 22.672519602336187 26.416118883266464 27.903480776482752 23.007880737914597 70-71 22.525482735973945 26.692243764081304 27.46329683089675 23.318976669048006 72-73 22.603871358248874 26.741569062623743 27.405616255696856 23.24894332343053 74-75 22.5466883251908 27.108154681391166 27.187778580707857 23.157378412710177 76-77 22.733575447633516 26.761556531424418 27.42081702596072 23.084050994981347 78-79 22.68963732952319 26.920649920427568 27.41275341193813 22.97695933811111 80-81 22.777067493478768 27.053562298795676 27.16079120867055 23.00857899905501 82-83 22.688379416911193 27.08734047975783 27.191841539952055 23.032438563378925 84-85 22.63257613126079 27.107881092950027 27.12725136522674 23.132291410562445 86-87 22.839152872711303 27.09683562546323 27.008701858409506 23.05530964341596 88-89 22.661615067230844 27.258889667228203 26.83994181155873 23.239553453982218 90-91 22.652770681589764 27.204417224058176 26.97094930239277 23.17186279195929 92-93 22.624265694913394 26.844855746795137 27.17930769441928 23.35157086387219 94-95 22.46895165468791 26.841437780124807 27.229143204981188 23.4604673602061 96-97 22.817423791542847 26.583801294673503 27.09321590879262 23.505559004991035 98-99 22.475968714550124 26.926157197238744 26.785421397606168 23.812452690604964 100-101 22.598861623579516 26.59846564866113 27.135896944954 23.666775782805356 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 718.0 1 489.5 2 402.0 3 535.5 4 433.0 5 404.0 6 814.5 7 1871.5 8 3096.0 9 3657.0 10 3105.5 11 2442.0 12 2459.5 13 2621.0 14 2853.5 15 3043.5 16 3256.0 17 3502.0 18 3770.0 19 4161.5 20 4624.0 21 5465.5 22 6691.0 23 8191.5 24 10178.5 25 12966.5 26 16692.0 27 20898.5 28 24676.5 29 28407.5 30 32767.0 31 38257.0 32 46078.0 33 50187.0 34 50976.0 35 53667.0 36 58206.0 37 63565.0 38 68006.5 39 71636.0 40 75892.5 41 82150.5 42 86700.5 43 89665.0 44 93232.5 45 98762.5 46 102599.5 47 105074.0 48 106370.0 49 104823.0 50 104613.0 51 103847.0 52 104393.5 53 107737.0 54 110838.5 55 105073.5 56 95315.0 57 88403.0 58 83236.5 59 74784.5 60 59933.0 61 49022.0 62 41116.0 63 31525.5 64 23066.0 65 17426.5 66 13166.0 67 10099.0 68 7678.5 69 5811.0 70 4586.5 71 3356.0 72 2412.5 73 1969.0 74 1493.5 75 1067.0 76 743.0 77 397.5 78 214.0 79 134.0 80 79.5 81 44.5 82 23.0 83 13.5 84 10.0 85 5.0 86 3.5 87 1.5 88 0.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014137059477903295 3 0.00627932496227258 4 3.4313251159959456E-5 5 3.4313251159959456E-5 6 0.0 7 0.0 8 3.77445762759554E-4 9 2.7450600927967565E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.088192604396351E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005490120185593513 26-27 0.021806071112154234 28-29 0.0 30-31 0.0 32-33 4.117590139195134E-4 34-35 0.01897522789145758 36-37 0.003448481741575925 38-39 0.0176884809729591 40-41 0.014617444994142727 42-43 0.007857734515630715 44-45 0.0025563372114169795 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7156625579979728E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.146987673993918E-5 84-85 0.0022818312021373036 86-87 2.058795069597567E-4 88-89 5.146987673993918E-5 90-91 2.401927581197162E-4 92-93 2.058795069597567E-4 94-95 0.0 96-97 1.7156625579979728E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2914326.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.33486430181512 #Duplication Level Percentage of deduplicated Percentage of total 1 72.369176195951 34.979543110654404 2 14.659388832633383 14.17119139945757 3 5.282199068820381 7.65943125619822 4 2.482120099566169 4.798917527733544 5 1.3749699795178245 3.3229493689531777 6 0.8494901405917962 2.463599440274058 7 0.5456073188351853 1.84602990025831 8 0.39327266829005547 1.5207024844330062 9 0.2959963690082888 1.287624989686107 >10 1.5510972212812826 13.8258487458979 >50 0.1261853453163022 4.184919370231485 >100 0.06359118822465681 5.512323248546865 >500 0.00405791342202747 1.3528064279902592 >1k 0.002634084151069686 2.215506991512852 >5k 1.423829270848479E-4 0.4997751579518934 >10k+ 7.119146354242394E-5 0.3588305802204865 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10428 0.3578185830960572 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8850 0.3036722727656412 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5674 0.19469338708160994 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3422 0.11741994546938127 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3322 0.1139886203533853 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3039 0.10427797027511677 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2963 0.10167016318695986 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3725300463983783E-4 2 0.0 0.0 0.0 0.0 1.3725300463983783E-4 3 0.0 0.0 0.0 0.0 1.3725300463983783E-4 4 0.0 0.0 0.0 0.0 1.3725300463983783E-4 5 0.0 0.0 0.0 0.0 1.3725300463983783E-4 6 0.0 0.0 0.0 0.0 1.3725300463983783E-4 7 0.0 0.0 0.0 0.0 1.3725300463983783E-4 8 0.0 0.0 0.0 0.0 1.7156625579979728E-4 9 0.0 0.0 0.0 0.0 1.7156625579979728E-4 10-11 0.0 0.0 0.0 0.0 1.7156625579979728E-4 12-13 0.0 0.0 0.0 0.0 2.7450600927967565E-4 14-15 0.0 0.0 0.0 0.0 3.9460238833953373E-4 16-17 0.0 0.0 0.0 5.1469876739939185E-5 4.460722650794729E-4 18-19 1.7156625579979728E-5 0.0 0.0 6.862650231991891E-5 4.803855162394324E-4 20-21 3.4313251159959456E-5 0.0 0.0 1.0293975347987837E-4 5.318553929793716E-4 22-23 3.4313251159959456E-5 0.0 0.0 2.573493836996959E-4 5.66168644139331E-4 24-25 3.4313251159959456E-5 0.0 0.0 4.117590139195135E-4 5.833252697193108E-4 26-27 3.4313251159959456E-5 0.0 0.0 7.892047766790675E-4 5.833252697193108E-4 28-29 3.4313251159959456E-5 0.0 0.0 0.0027965299695366956 5.833252697193108E-4 30-31 6.862650231991891E-5 0.0 0.0 0.009950842836388241 5.833252697193108E-4 32-33 8.578312789989864E-5 0.0 0.0 0.026506986521068678 6.862650231991891E-4 34-35 1.200963790598581E-4 0.0 0.0 0.051675756246898935 6.862650231991891E-4 36-37 1.3725300463983783E-4 0.0 0.0 0.09293744076675019 6.862650231991891E-4 38-39 1.7156625579979728E-4 0.0 0.0 0.1536890519454584 7.205782743591486E-4 40-41 1.88722881379777E-4 0.0 0.0 0.21999940981208005 7.54891525519108E-4 42-43 2.2303613253973648E-4 0.0 0.0 0.28660143031356133 7.54891525519108E-4 44-45 2.401927581197162E-4 0.0 0.0 0.36795814881382527 8.23518027839027E-4 46-47 2.9166263485965534E-4 0.0 0.0 0.4617362642339944 9.093011557389256E-4 48-49 3.088192604396351E-4 0.0 0.0 0.5606956805793175 9.264577813189053E-4 50-51 3.088192604396351E-4 0.0 0.0 0.6698804457703085 0.0010293975347987836 52-53 3.088192604396351E-4 0.0 0.0 0.7724770667385873 0.0010980240371187026 54-55 3.4313251159959456E-4 0.0 0.0 0.8816275186784184 0.001132337288278662 56-57 3.4313251159959456E-4 0.0 0.0 1.006253246891391 0.0011838071650186013 58-59 3.4313251159959456E-4 0.0 0.0 1.1415160829639512 0.001200963790598581 60-61 3.4313251159959456E-4 0.0 0.0 1.2844994005475021 0.0012181204161785606 62-63 3.4313251159959456E-4 0.0 0.0 1.4276028145101132 0.001389686671978358 64-65 3.4313251159959456E-4 0.0 0.0 1.57410324033756 0.0014239999231383175 66-67 3.4313251159959456E-4 0.0 0.0 1.7449489178629982 0.0014411565487182972 68-69 3.4313251159959456E-4 0.0 0.0 1.9283875585641415 0.0014411565487182972 70-71 3.602891371795743E-4 0.0 0.0 2.119855500036715 0.0014926264254582363 72-73 3.77445762759554E-4 0.0 0.0 2.3159385737903033 0.0016127228045180945 74-75 3.77445762759554E-4 0.0 0.0 2.5154529726598875 0.0017156625579979727 76-77 3.77445762759554E-4 0.0 0.0 2.732964671762871 0.001767132434737912 78-79 3.77445762759554E-4 0.0 0.0 2.97077265892697 0.0018529155626378106 80-81 3.77445762759554E-4 0.0 0.0 3.2222544766783123 0.0019043854393777497 82-83 3.77445762759554E-4 0.0 0.0 3.481010703675567 0.001955855316117689 84-85 4.2891563949949316E-4 0.0 0.0 3.7412252438471194 0.001973011941697669 86-87 4.460722650794729E-4 0.0 0.0 4.014118530322277 0.002075951695177547 88-89 4.460722650794729E-4 0.0 0.0 4.306776249465571 0.0021788914486574255 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4845 0.0 52.55347 1 GTATCAA 8495 0.0 42.499187 1 ATCAACG 10800 0.0 33.16143 3 TCAACGC 10920 0.0 32.88289 4 TATCAAC 11080 0.0 32.326748 2 CAACGCA 11155 0.0 32.104992 5 AACGCAG 11495 0.0 31.196709 6 TGGTATC 2165 0.0 28.085522 2 ACGCAGA 13155 0.0 27.115639 7 CGCAGAG 13255 0.0 26.911072 8 TACATGG 8035 0.0 26.249891 2 GTGGTAT 2415 0.0 25.96493 1 GTACATG 8365 0.0 25.952585 1 TAAGGTG 1475 0.0 25.43934 5 ACATGGG 8230 0.0 24.644144 3 GCAGAGT 15135 0.0 23.223095 9 GTAAGGT 1540 0.0 21.8982 4 CATGGGG 5565 0.0 21.849705 4 AGAGTAC 13545 0.0 21.530617 10-11 GAGTACT 10200 0.0 21.420444 12-13 >>END_MODULE