FastQCFastQC Report
Thu 26 May 2016
SRR937948_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937948_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972843
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106410.35794019394902454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84370.2838024073252439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51010.1715865923629334No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37570.12637734316948457No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA36330.12220625172604137No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31250.1051182319416128No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30420.10232629170124356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.054.394691
GTATCAA92200.042.3087581
ATCAACG112050.034.5084573
TATCAAC112850.034.346962
TCAACGC113950.033.9330644
CAACGCA115650.033.5165145
AACGCAG119550.032.5773126
ACGCAGA136050.028.3405367
CGCAGAG136150.028.2135338
TGGTATC22800.026.2825742
GTGGTAT24700.025.2610631
GCAGAGT151350.025.002719
GAGTACT96500.023.152112-13
AGGACCG10850.022.7939955
GTACATG90050.022.7437461
TAAGGTG16950.022.728055
TACATGG88850.022.2673282
CAGAGTA145550.021.97956310-11
GTACTTT102500.021.6097114-15
ACATGGG89600.021.0731473
Produced by FastQC (version 0.11.5)