FastQCFastQC Report
Thu 26 May 2016
SRR937948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972843
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102910.3461669519715639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85330.2870316394104902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54390.18295618032973823No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35520.11948158715411478No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34750.11689147391907342No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31070.1045127509256291No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30650.10309996188833384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50500.053.4293941
GTATCAA85200.041.816241
ATCAACG107050.032.9211353
TCAACGC109450.032.195454
CAACGCA110150.032.077085
TATCAAC110300.032.0404662
AACGCAG115150.030.7254856
ACGCAGA133200.026.3831467
CGCAGAG134000.026.1901978
ACCGTAC950.001228570524.994568
TGGTATC21800.023.9683192
GTGGTAT23100.023.8544451
GTACATG84500.023.66731
TACATGG82500.023.5489062
GTAAGGT15400.023.1284714
TAAGGTG17050.023.1185255
AGGTAAG15250.023.0495912
GCAGAGT151900.022.634979
TATACCG1703.1572727E-622.3484545
ACATGGG83050.021.960853
Produced by FastQC (version 0.11.5)