FastQCFastQC Report
Thu 26 May 2016
SRR937947_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937947_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569694
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61350.390840507767756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49720.3167496340050991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31110.19819149464800145No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17200.10957549688028367No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16790.10696352282674204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.056.067451
GTATCAA46950.042.250691
TGGTATC11000.034.129222
ATCAACG60050.032.5252573
TATCAAC60550.032.2566762
TCAACGC61050.031.9146524
CAACGCA63050.030.9022925
GTGGTAT12450.029.8745461
AACGCAG65850.029.6566856
ACGCAGA75250.025.7577027
CGCAGAG76350.025.3866048
GTACGCT1000.00164952323.7608493
TATACCG1201.7059139E-423.7608475
GCAGAGT84000.022.6214459
GTACATG49200.022.2914751
GAGTACT53250.022.16901612-13
TACATGG46900.021.6836742
CAGAGTA78750.020.72091510-11
GGACCAT7600.020.631796
GTACTTT56950.020.6032614-15
Produced by FastQC (version 0.11.5)