FastQCFastQC Report
Thu 26 May 2016
SRR937947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569694
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59860.38134821181708023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50950.324585556165724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32880.2094675777571934No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17680.11263341772345438No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA15700.10001949424537522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28450.055.34091
GTATCAA52650.043.7267841
ATCAACG66250.034.2434543
TCAACGC67250.033.734264
TATCAAC67900.033.415592
CAACGCA68450.033.1407475
AACGCAG70700.032.109286
TGGTATC12700.029.5885642
GGCGTAA650.00584941929.2259586
GTGGTAT13850.028.1620711
ACGCAGA82400.027.319537
CGCAGAG84300.026.5347758
GCAGAGT92500.024.0284779
TAAGGTG9550.022.409215
GAGTACT61950.021.42706112-13
AGAGTAC82250.021.13329710-11
CAGAGTA87700.021.0384410-11
GTAAGGT8400.020.9492054
GTACTTT63700.020.7638514-15
AGTACTT62900.020.27289412-13
Produced by FastQC (version 0.11.5)