FastQCFastQC Report
Thu 26 May 2016
SRR937946_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937946_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548816
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62480.4034049235028564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49980.32269811262280346No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31470.20318746707162116No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16980.10963213189946386No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA16580.10704951395130216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26600.053.3105281
GTATCAA49150.043.326091
ATCAACG61800.034.1478083
TATCAAC62850.033.8041952
TCAACGC63650.033.0806244
CAACGCA64350.032.720775
AACGCAG66900.031.6836346
TGGTATC12250.030.2640382
CGCAGAG76750.027.4811158
ACGCAGA76650.027.4549947
GTGGTAT14500.026.6719741
GCAGAGT84650.024.5228129
GTATAGG4250.022.4687651
TCCGGAC2354.618414E-922.2481143
GTGTAAG7300.022.2379231
GTACATG46400.021.9179421
AGAGTAC77500.021.82117310-11
TACATGG45500.021.7280272
TGACGCG550.002862800821.6112194-95
TAGCCCT10800.021.1244724
Produced by FastQC (version 0.11.5)